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The map label for this gene is cspR [H]

Identifier: 78223582

GI number: 78223582

Start: 2689561

End: 2690037

Strand: Direct

Name: cspR [H]

Synonym: Gmet_2379

Alternate gene names: 78223582

Gene position: 2689561-2690037 (Clockwise)

Preceding gene: 78223578

Following gene: 78223583

Centisome position: 67.28

GC content: 59.33

Gene sequence:

>477_bases
ATGCCTGAAAAGCCACCATTCCACATCGTCCTCATCGAGCCGGAAATCCCCCCTAACACCGGCAACATCGCCCGCCTCTG
CGGCGCAACCGGCACCGTGCTTCACCTGGTGGGGAAACTGGGCTTTTCCACTGACGACCGCTATCTCAAGCGGGCGGGGC
TCGACTACTGGAGCGAGGTGGAGATCCACTACTGGGACGACCTTGCAGCGCTTCAGCACGCCTATCCGGAGGGCCGATTC
ATCTATACGAGCAAGAAAGCGGCGAAAACACACATCGAAGCGGCGTTCAAGGAGGGGGACTTCATCGTTTTCGGGAAGGA
GACGGTGGGGCTGCCTGAGGAACTTATCGAGGCCAATCGGGAAAACACCGTGAGAATCCCCATTTTCGGCAAGGTCCGCA
GCCTCAACCTCTCAACCTCGGCGGGAATCGTTCTTTACGAGGCACTCCGCCAGACCGGGAGGCTCGAAGGATGCTGA

Upstream 100 bases:

>100_bases
GAGACTGCGAAGAAGCGTTAATAATAGACCCGTCGACCGGAAAATGCAAGACCGGCGACCACCGTCACCCGTATACGGCA
AGCCGTCAGAAGGAAAGAAG

Downstream 100 bases:

>100_bases
AGCTCGGCATCATCTACATGAACCTGGCGCTTCTCTTCTACACTTACGCCGTATTCAACGGCAGGAGGGAGGGGCTTCAC
CGCAAGCACCTCATAGTGTT

Product: tRNA/rRNA methyltransferase (SpoU)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MPEKPPFHIVLIEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEVEIHYWDDLAALQHAYPEGRF
IYTSKKAAKTHIEAAFKEGDFIVFGKETVGLPEELIEANRENTVRIPIFGKVRSLNLSTSAGIVLYEALRQTGRLEGC

Sequences:

>Translated_158_residues
MPEKPPFHIVLIEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEVEIHYWDDLAALQHAYPEGRF
IYTSKKAAKTHIEAAFKEGDFIVFGKETVGLPEELIEANRENTVRIPIFGKVRSLNLSTSAGIVLYEALRQTGRLEGC
>Mature_157_residues
PEKPPFHIVLIEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEVEIHYWDDLAALQHAYPEGRFI
YTSKKAAKTHIEAAFKEGDFIVFGKETVGLPEELIEANRENTVRIPIFGKVRSLNLSTSAGIVLYEALRQTGRLEGC

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=148, Percent_Identity=43.2432432432432, Blast_Score=128, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17627; Mature: 17496

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEKPPFHIVLIEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEV
CCCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCEE
EIHYWDDLAALQHAYPEGRFIYTSKKAAKTHIEAAFKEGDFIVFGKETVGLPEELIEANR
EEEECCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHCCC
ENTVRIPIFGKVRSLNLSTSAGIVLYEALRQTGRLEGC
CCEEEEEEECCEEEECCCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PEKPPFHIVLIEPEIPPNTGNIARLCGATGTVLHLVGKLGFSTDDRYLKRAGLDYWSEV
CCCCCEEEEEECCCCCCCCCCHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCEE
EIHYWDDLAALQHAYPEGRFIYTSKKAAKTHIEAAFKEGDFIVFGKETVGLPEELIEANR
EEEECCHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHCCC
ENTVRIPIFGKVRSLNLSTSAGIVLYEALRQTGRLEGC
CCEEEEEEECCEEEECCCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]