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The map label for this gene is capI [H]

Identifier: 78223533

GI number: 78223533

Start: 2637313

End: 2638323

Strand: Reverse

Name: capI [H]

Synonym: Gmet_2330

Alternate gene names: 78223533

Gene position: 2638323-2637313 (Counterclockwise)

Preceding gene: 78223534

Following gene: 78223532

Centisome position: 66.0

GC content: 61.23

Gene sequence:

>1011_bases
ATGTCCACGGTTCTCGTCACCGGCGCCGCCGGCTTCATCGGATTTCACCTCTCCCAGAGACTCCTCGCTCGGGGCGACCG
CGTGGTGGGTCTCGACAATCTCAACGACTACTACGATGTAAATCTGAAGCTGGACCGGCTTCGCCAGTTGGAGGGGCGGG
AGGGGTTCCGTTTTATCCGGGCGAGCCTGGCTGACCGACCGGCCCTGGAAGAGCTCTTTAGCGGCGAGCGCTTCGACGCG
GTGGTGAACCTAGCGGCCCAGGCCGGGGTCCGTTATTCCCTCAAGAATCCCCACGCCTACGTGGAGAGCAATCTGGTCGG
CTTTATGAACATCCTGGAAGGGTGCCGCCACCACGGCGTGAAGCATCTGGTCTACGCCTCGTCCAGCTCCGTCTACGGGG
CCAACACGGCCATGCCCTTTTCAGTCCACCACAACGTGGACCATCCGGTCTCCCTCTACGCGGCCACCAAGAAGGCCAAC
GAACTCATGGCCCACACCTATTCGAGCCTCTACGGACTTCCTACTACGGGATTGCGGTTCTTCACGGTTTACGGACCCTG
GGGGCGGCCCGACATGGCGCTCTTCCTCTTTACCAAGGCTATCCTTGAGGGGCGTCCCATAGACGTCTACAACCACGGCA
AAATGCAGCGGGACTTCACCTACATCGACGACATCGTCGAGGGGGTCATGCGGGTCATGGACCGGACCGCCGAGCCCAAC
CCATCCTGGAGCGGCGACCATCCCGACCCGGGCACCAGCTACGCCCCCTACCGGATCTACAACATCGGCAACAACAGCCC
GGTGGAGCTCTTGACGTTCATCGAGACCATCGAGAAGTGCATCGGCAAGCCGGCGGAGAAGAACTTCCTCCCCATTCAGG
CGGGGGACGTGCCGGCCACCTACGCCGACGTGGACGATCTGATGAACGATGTGGGTTTCAAACCGGCCACTCCCATCGGG
GAAGGGATACGGCGGTTTGTTGAGTGGTATCGGGAGTATTACCATGTCTGA

Upstream 100 bases:

>100_bases
AGGCATCGAAAAGCTCCTGACGCCCCCCTTTCCATCGGCCGGGTGATGTGTTACACTGCCTCCTCTTCAATAGCCCGATA
AATCAGAAGGATAGCGCACC

Downstream 100 bases:

>100_bases
GGTCAGGAAAGGAGAAGGGATGACTGTTCTCGTTACTGGCGGTGCCGGGTACATCGGGAGCCATGTGGTCCGACAGCTTT
CCGAGGCCGGCTATACCGTG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSTVLVTGAAGFIGFHLSQRLLARGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFRFIRASLADRPALEELFSGERFDA
VVNLAAQAGVRYSLKNPHAYVESNLVGFMNILEGCRHHGVKHLVYASSSSVYGANTAMPFSVHHNVDHPVSLYAATKKAN
ELMAHTYSSLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVYNHGKMQRDFTYIDDIVEGVMRVMDRTAEPN
PSWSGDHPDPGTSYAPYRIYNIGNNSPVELLTFIETIEKCIGKPAEKNFLPIQAGDVPATYADVDDLMNDVGFKPATPIG
EGIRRFVEWYREYYHV

Sequences:

>Translated_336_residues
MSTVLVTGAAGFIGFHLSQRLLARGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFRFIRASLADRPALEELFSGERFDA
VVNLAAQAGVRYSLKNPHAYVESNLVGFMNILEGCRHHGVKHLVYASSSSVYGANTAMPFSVHHNVDHPVSLYAATKKAN
ELMAHTYSSLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVYNHGKMQRDFTYIDDIVEGVMRVMDRTAEPN
PSWSGDHPDPGTSYAPYRIYNIGNNSPVELLTFIETIEKCIGKPAEKNFLPIQAGDVPATYADVDDLMNDVGFKPATPIG
EGIRRFVEWYREYYHV
>Mature_335_residues
STVLVTGAAGFIGFHLSQRLLARGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFRFIRASLADRPALEELFSGERFDAV
VNLAAQAGVRYSLKNPHAYVESNLVGFMNILEGCRHHGVKHLVYASSSSVYGANTAMPFSVHHNVDHPVSLYAATKKANE
LMAHTYSSLYGLPTTGLRFFTVYGPWGRPDMALFLFTKAILEGRPIDVYNHGKMQRDFTYIDDIVEGVMRVMDRTAEPNP
SWSGDHPDPGTSYAPYRIYNIGNNSPVELLTFIETIEKCIGKPAEKNFLPIQAGDVPATYADVDDLMNDVGFKPATPIGE
GIRRFVEWYREYYHV

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=26.9794721407625, Blast_Score=129, Evalue=5e-30,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=26.0355029585799, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI56237023, Length=351, Percent_Identity=25.9259259259259, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI56118217, Length=351, Percent_Identity=25.9259259259259, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI189083684, Length=351, Percent_Identity=25.9259259259259, Blast_Score=108, Evalue=9e-24,
Organism=Escherichia coli, GI1788353, Length=354, Percent_Identity=27.683615819209, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI48994969, Length=353, Percent_Identity=27.7620396600567, Blast_Score=107, Evalue=9e-25,
Organism=Escherichia coli, GI1786974, Length=349, Percent_Identity=23.7822349570201, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788589, Length=249, Percent_Identity=26.1044176706827, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=349, Percent_Identity=25.5014326647564, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71982038, Length=351, Percent_Identity=25.3561253561254, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17568069, Length=348, Percent_Identity=27.2988505747126, Blast_Score=107, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI17539532, Length=335, Percent_Identity=24.4776119402985, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115532424, Length=350, Percent_Identity=22.5714285714286, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=137, Percent_Identity=35.036496350365, Blast_Score=76, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19923002, Length=352, Percent_Identity=26.7045454545455, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=24.9258160237389, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37695; Mature: 37564

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVLVTGAAGFIGFHLSQRLLARGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFRFIR
CCEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECEEHHHHHHHCCCCHHHHHH
ASLADRPALEELFSGERFDAVVNLAAQAGVRYSLKNPHAYVESNLVGFMNILEGCRHHGV
HHHCCCHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
KHLVYASSSSVYGANTAMPFSVHHNVDHPVSLYAATKKANELMAHTYSSLYGLPTTGLRF
EEEEEECCCCCCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
FTVYGPWGRPDMALFLFTKAILEGRPIDVYNHGKMQRDFTYIDDIVEGVMRVMDRTAEPN
EEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
PSWSGDHPDPGTSYAPYRIYNIGNNSPVELLTFIETIEKCIGKPAEKNFLPIQAGDVPAT
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCH
YADVDDLMNDVGFKPATPIGEGIRRFVEWYREYYHV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
STVLVTGAAGFIGFHLSQRLLARGDRVVGLDNLNDYYDVNLKLDRLRQLEGREGFRFIR
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCCCEEEECEEHHHHHHHCCCCHHHHHH
ASLADRPALEELFSGERFDAVVNLAAQAGVRYSLKNPHAYVESNLVGFMNILEGCRHHGV
HHHCCCHHHHHHHCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCC
KHLVYASSSSVYGANTAMPFSVHHNVDHPVSLYAATKKANELMAHTYSSLYGLPTTGLRF
EEEEEECCCCCCCCCCCCCEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
FTVYGPWGRPDMALFLFTKAILEGRPIDVYNHGKMQRDFTYIDDIVEGVMRVMDRTAEPN
EEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
PSWSGDHPDPGTSYAPYRIYNIGNNSPVELLTFIETIEKCIGKPAEKNFLPIQAGDVPAT
CCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCH
YADVDDLMNDVGFKPATPIGEGIRRFVEWYREYYHV
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]