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The map label for this gene is tmk [H]

Identifier: 78223521

GI number: 78223521

Start: 2626564

End: 2627217

Strand: Direct

Name: tmk [H]

Synonym: Gmet_2318

Alternate gene names: 78223521

Gene position: 2626564-2627217 (Clockwise)

Preceding gene: 78223500

Following gene: 78223522

Centisome position: 65.71

GC content: 66.97

Gene sequence:

>654_bases
ATGGGTTTTTTCATCACATTCGAAGGAATCGAGGGATGCGGCAAGTCGACCCAGGTCCGACTGGTCGCCGAACGGCTCAG
CCGGGCCGGCCACGGGGTGGTGGTGACCCGCGAACCGGGAGGATGCCCCATCGCCGACGCCATCAGGGCAATTCTTCTGG
ACGCCCGCAACAGCGCCATGGTGCCCATGGCCGAACTCCTCCTCTACGCGGCGGCCCGGGCCCAGCACGTGGCGGAGGTG
ATCGAACCGGCCCTGGCAGCGGGGAAGATCGTCCTCTGCGATCGCTTCACCGACTCCACCATGGCCTACCAGGGATTCGG
CCGCAAGCTGGACCGGGAACTGATTGCCCAGCTCAACAGCCTGGCCGCCGGCAGCATCAGACCGGATCTGACCCTCCTTC
TGGACTGTTCCGTGGAGACGGGGCTCGCCCGGGCCATGGCCCGGATCAACACAAGCAGCGCGGCCCGGGAGGAGCGGTTC
GAGCAGGAGTCCATCCTCTTCCACGAGCGGATCAGGGACGGCTTCCTCTCCCTCGCCGCTGCCGAACCGGGGCGGTTCGT
GGTTCTGGACGGCAGTGGCGGCATCGAAGAGACCGAGGCCCTCGTGACGGAGGCGATCCTTGCCCGCCTTCCCGCATCCC
CTGCAAGGAGGTAG

Upstream 100 bases:

>100_bases
ATCAATAATTATGCGACTTGTCGGGGATTTTTGCCTTGATTTACCCTTGCCACTTCCCTATAATTCAGCTTTTACAAAAT
GGAAGGGCAATGCAACCCCC

Downstream 100 bases:

>100_bases
GCGGTGCCCTTCTCCCGCGTCATTGGCCAGGATACCCCCGTCAACGTTCTGCGGCGTGCGCTTCGCTCCGGCAAGACGGC
CCATGCCTACCTGTTCGAGG

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MGFFITFEGIEGCGKSTQVRLVAERLSRAGHGVVVTREPGGCPIADAIRAILLDARNSAMVPMAELLLYAAARAQHVAEV
IEPALAAGKIVLCDRFTDSTMAYQGFGRKLDRELIAQLNSLAAGSIRPDLTLLLDCSVETGLARAMARINTSSAAREERF
EQESILFHERIRDGFLSLAAAEPGRFVVLDGSGGIEETEALVTEAILARLPASPARR

Sequences:

>Translated_217_residues
MGFFITFEGIEGCGKSTQVRLVAERLSRAGHGVVVTREPGGCPIADAIRAILLDARNSAMVPMAELLLYAAARAQHVAEV
IEPALAAGKIVLCDRFTDSTMAYQGFGRKLDRELIAQLNSLAAGSIRPDLTLLLDCSVETGLARAMARINTSSAAREERF
EQESILFHERIRDGFLSLAAAEPGRFVVLDGSGGIEETEALVTEAILARLPASPARR
>Mature_216_residues
GFFITFEGIEGCGKSTQVRLVAERLSRAGHGVVVTREPGGCPIADAIRAILLDARNSAMVPMAELLLYAAARAQHVAEVI
EPALAAGKIVLCDRFTDSTMAYQGFGRKLDRELIAQLNSLAAGSIRPDLTLLLDCSVETGLARAMARINTSSAAREERFE
QESILFHERIRDGFLSLAAAEPGRFVVLDGSGGIEETEALVTEAILARLPASPARR

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=183, Percent_Identity=43.7158469945355, Blast_Score=137, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 23280; Mature: 23149

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFFITFEGIEGCGKSTQVRLVAERLSRAGHGVVVTREPGGCPIADAIRAILLDARNSAM
CCEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCC
VPMAELLLYAAARAQHVAEVIEPALAAGKIVLCDRFTDSTMAYQGFGRKLDRELIAQLNS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LAAGSIRPDLTLLLDCSVETGLARAMARINTSSAAREERFEQESILFHERIRDGFLSLAA
HHCCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEPGRFVVLDGSGGIEETEALVTEAILARLPASPARR
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GFFITFEGIEGCGKSTQVRLVAERLSRAGHGVVVTREPGGCPIADAIRAILLDARNSAM
CEEEEECCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCC
VPMAELLLYAAARAQHVAEVIEPALAAGKIVLCDRFTDSTMAYQGFGRKLDRELIAQLNS
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LAAGSIRPDLTLLLDCSVETGLARAMARINTSSAAREERFEQESILFHERIRDGFLSLAA
HHCCCCCCCEEEEEECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
AEPGRFVVLDGSGGIEETEALVTEAILARLPASPARR
CCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA