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The map label for this gene is holB [H]

Identifier: 78223522

GI number: 78223522

Start: 2627221

End: 2628177

Strand: Direct

Name: holB [H]

Synonym: Gmet_2319

Alternate gene names: 78223522

Gene position: 2627221-2628177 (Clockwise)

Preceding gene: 78223521

Following gene: 78223523

Centisome position: 65.72

GC content: 66.25

Gene sequence:

>957_bases
GTGCCCTTCTCCCGCGTCATTGGCCAGGATACCCCCGTCAACGTTCTGCGGCGTGCGCTTCGCTCCGGCAAGACGGCCCA
TGCCTACCTGTTCGAGGGAATCGGGGGATGCGGCAAGGGAACCACGGCTCTCGCTTTTGTGGAGGCTGCCTTCTGCGGCC
GGGATGATGGGTGCGGCACCTGCCCCTCCTGCCGGAAGATGGCGACGCTGCAGCACCCCGATCTCCACCCGGTGGAACCC
GACGGGGCGTTCATCAAGATAGACCAGGTCCGGGAACTCCAGCGGGAACTGGTGCTTCGCCCCATCGAGGCGCCGCTCAA
GGCATGCATCATCAGGGATGCGGACCGGCTCAATCCGGCAGCAGGCAACGCCCTCCTGAAGACCCTGGAGGAACCGCCGG
GCAATGCCCTGATGATCCTCCTCACCACGAACCTGGACGGGGTCCTTCCCACGATCCGCTCCCGCTGCCAGCTCCTGCGC
TTTGCGCCCCTTCCCGAGGAGCTCATCGAGGAGCGACTGGTGGCAGCCGGACGGGACCGGGCCGCAGCACGCCTTGCCGC
ATCCCTTGCGGGTGGAAGCCTGGGGAAAGCCCTTGGGGATGAGGAAGCGGACACGGTGGACCGGCAAGCGCTCCTGGAAC
GGCTCAGGACCCTCTCCCTCAGCGACACGGCCTCCCTCTTCGCCGCTGCCGAAGAGATGGCCGCCAACCGCGAACAGGCC
CTGAAGCAGTTGGACGTGCTCGCGTCGCTTCTGCGCGACATCCTCCTCATCCAGGGGGGGAGCGATGCCGTGGTCAACCG
CGACATCATGCCATTTCTGGAAAGCGAGGCGGCGCGCCTGTCGCGGGAAAAGAATCTCGAACGAATAAGCAACACCATGG
AGGCCCGCCAGGCCCTCATGCGCAACGCCAACCCGCGGCTCACCCTCGACGTGCTCCTGATGCGTCTGGCGGGGTAA

Upstream 100 bases:

>100_bases
CGTGGTTCTGGACGGCAGTGGCGGCATCGAAGAGACCGAGGCCCTCGTGACGGAGGCGATCCTTGCCCGCCTTCCCGCAT
CCCCTGCAAGGAGGTAGGCG

Downstream 100 bases:

>100_bases
ACGCCGCTTGTATTTTTTTCGGAGGACAACTTGGTAAAGATTGTGAAGGCCCAGTTCCACACCGCCGGCAAACTCTACGA
CTTCGGGGCGGGCGATCTCG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MPFSRVIGQDTPVNVLRRALRSGKTAHAYLFEGIGGCGKGTTALAFVEAAFCGRDDGCGTCPSCRKMATLQHPDLHPVEP
DGAFIKIDQVRELQRELVLRPIEAPLKACIIRDADRLNPAAGNALLKTLEEPPGNALMILLTTNLDGVLPTIRSRCQLLR
FAPLPEELIEERLVAAGRDRAAARLAASLAGGSLGKALGDEEADTVDRQALLERLRTLSLSDTASLFAAAEEMAANREQA
LKQLDVLASLLRDILLIQGGSDAVVNRDIMPFLESEAARLSREKNLERISNTMEARQALMRNANPRLTLDVLLMRLAG

Sequences:

>Translated_318_residues
MPFSRVIGQDTPVNVLRRALRSGKTAHAYLFEGIGGCGKGTTALAFVEAAFCGRDDGCGTCPSCRKMATLQHPDLHPVEP
DGAFIKIDQVRELQRELVLRPIEAPLKACIIRDADRLNPAAGNALLKTLEEPPGNALMILLTTNLDGVLPTIRSRCQLLR
FAPLPEELIEERLVAAGRDRAAARLAASLAGGSLGKALGDEEADTVDRQALLERLRTLSLSDTASLFAAAEEMAANREQA
LKQLDVLASLLRDILLIQGGSDAVVNRDIMPFLESEAARLSREKNLERISNTMEARQALMRNANPRLTLDVLLMRLAG
>Mature_317_residues
PFSRVIGQDTPVNVLRRALRSGKTAHAYLFEGIGGCGKGTTALAFVEAAFCGRDDGCGTCPSCRKMATLQHPDLHPVEPD
GAFIKIDQVRELQRELVLRPIEAPLKACIIRDADRLNPAAGNALLKTLEEPPGNALMILLTTNLDGVLPTIRSRCQLLRF
APLPEELIEERLVAAGRDRAAARLAASLAGGSLGKALGDEEADTVDRQALLERLRTLSLSDTASLFAAAEEMAANREQAL
KQLDVLASLLRDILLIQGGSDAVVNRDIMPFLESEAARLSREKNLERISNTMEARQALMRNANPRLTLDVLLMRLAG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=310, Percent_Identity=31.9354838709677, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786676, Length=258, Percent_Identity=31.7829457364341, Blast_Score=85, Evalue=7e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004622 [H]

Pfam domain/function: NA

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 34461; Mature: 34330

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFSRVIGQDTPVNVLRRALRSGKTAHAYLFEGIGGCGKGTTALAFVEAAFCGRDDGCGT
CCCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCC
CPSCRKMATLQHPDLHPVEPDGAFIKIDQVRELQRELVLRPIEAPLKACIIRDADRLNPA
CHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCH
AGNALLKTLEEPPGNALMILLTTNLDGVLPTIRSRCQLLRFAPLPEELIEERLVAAGRDR
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHH
AAARLAASLAGGSLGKALGDEEADTVDRQALLERLRTLSLSDTASLFAAAEEMAANREQA
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHH
LKQLDVLASLLRDILLIQGGSDAVVNRDIMPFLESEAARLSREKNLERISNTMEARQALM
HHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNANPRLTLDVLLMRLAG
HCCCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PFSRVIGQDTPVNVLRRALRSGKTAHAYLFEGIGGCGKGTTALAFVEAAFCGRDDGCGT
CCHHHCCCCCCHHHHHHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCC
CPSCRKMATLQHPDLHPVEPDGAFIKIDQVRELQRELVLRPIEAPLKACIIRDADRLNPA
CHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCHHHCCCH
AGNALLKTLEEPPGNALMILLTTNLDGVLPTIRSRCQLLRFAPLPEELIEERLVAAGRDR
HHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHH
AAARLAASLAGGSLGKALGDEEADTVDRQALLERLRTLSLSDTASLFAAAEEMAANREQA
HHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHH
LKQLDVLASLLRDILLIQGGSDAVVNRDIMPFLESEAARLSREKNLERISNTMEARQALM
HHHHHHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNANPRLTLDVLLMRLAG
HCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]