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The map label for this gene is kstD [H]

Identifier: 78223430

GI number: 78223430

Start: 2516692

End: 2518302

Strand: Direct

Name: kstD [H]

Synonym: Gmet_2225

Alternate gene names: 78223430

Gene position: 2516692-2518302 (Clockwise)

Preceding gene: 78223429

Following gene: 78223431

Centisome position: 62.96

GC content: 55.25

Gene sequence:

>1611_bases
ATGATAATCAAGAACTTACCAAAGAAATGCGATATTTTAATCATTGGTTCGGGTGCGGCCGGACTTACGTTTGCGCTCGC
CGTAAAAAAGTTTAAACCGAATTTGCACGTTCACGTGGTGGAAAAGACCGAGAGTGTCGGCGGGTGTACGGCATACAGCG
GCGGAGGAGTCTGGCTCCCCGGCCACCGGTTCATGGCAGACCCTTCCCAGGACACCGAAGCGGCCCGCCGGTATGTCAAA
AATGTTTACCCGGAGATTGACGAAAAATGCCTCGAAGGATTCCTTGCTGATGCACCAAACCTGTTGGATTTTTGGATCGC
CAATGGTGTCGAGATGGAGAAATCCATCGGCTACCCCGATTACTACATGGAGAAGGAAGGCTCCGCCAGGGAGAGATCGG
TTTTCCCCGGAGTCTACAAGGGGCCAAAGAAATACCGCTCCCTGGTCCGCAAAACCCCCGCCTATTATGTCCCCTTTACC
CTTAATGAGGCTATGACCTGGGGTGTGCACCGTTTCGGGCACTGGAACAAGACTCTGTTGACCAAACGAGCCGTTGCCGG
ACATATGACCATGGGAAAGGCATTCATTGCTTTCCTGCTGGAGGCGTGCATCGAGGCCGGTGTTGATCTCTCCCTCAACA
GCACTACCGAAAGGCTGGAAATAGAGAATGGCACCGTGACCGGGGCGATAATAAACGGTCAGCGGATAACGGCGCCGGTA
GTCATGATGGCAAACGGCGGTTTCTCCCACAACCCTGAACTGATGAAGCGGATCGACGCCGAGAGACCCATTCTCTCCGT
AGCCCCCGAGGAGTGCGATACCGGCAACGGAGGGCTGGGCCTTGCACTGGAAGCCGGGCTCAAGATCGGCAATCCGTACT
GCTGGTGGTCACCTGTCTTCAAAACCTACGATGACCGGATCGAGGAAAAACCGGGACCGGATCTCTGGGCCTATCACAGT
GCTCTCTATGACCGTTGCTGGCCTGGAGGCATCATGGTCGATGGCAACGGCAAACGGTTTACCAATGAATCAGCCTGTTA
CAATACTGTCGGCGGAGTGCTTGCCCAGGGAAAGGATCCGGCACTTAACAACGTATGGCTCATCTGGGGCGACTACTACG
TCAAAAACTACGTCAGGGGAATCGTCTCTTACCTGCAACCTGCCAAGAGCTACATGAACAAATCAAAGACACTCGAAGAT
CTGGCGGACAAAATCGGAATCCCTGCGGCAAACCTCAGGGAAACGGTGGAGCATTGGAACTCCATGGCAGGAAAGCAAAA
AGACGACGATTTCCACCGGGGAGAGAGTCCGTACGACAATTATATGGGAGACAAGTTCAGAAACGGCCACCCCAATATCG
AGAAGGTCGAAGGGCCATACCAGGCGGTGCGGATGCATGCTGGTACGCTCGGAACGAAGATGGGGCCGATCACCGATGAG
TACGGTCGCACTCAGCTGGAAAACGGCCAATTCGTGACCGGACTCTACGCCTCGGGAAACGCTGCCGCTTCCTTCTTCGG
GAACTTCTACCCCGGCGCCGGCGCAACCCTGGGACAGGGGTGCGTCTTAGCGTATCGTGCCGCGCGTCATGCCTGCGGCC
AAGGGGATTGA

Upstream 100 bases:

>100_bases
TGAGGATTCGAGCAATGCAATCAAGGCCTTTATTGAAAAACGAACTCCGGTTTTCAAATAGGAATAAATTCTAAAGATCT
CAAAACAACAGGAGAAAATC

Downstream 100 bases:

>100_bases
AACCACTGGGCCGCAAATTTTATAAACCGATGATAACAGGAGGAACAGATGGGAAAACTCGCCGGAAAGGTGGCAATTAT
TACCGGTGGATCGCGCGGCA

Product: fumarate reductase/succinate dehydrogenase flavoprotein-like

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MIIKNLPKKCDILIIGSGAAGLTFALAVKKFKPNLHVHVVEKTESVGGCTAYSGGGVWLPGHRFMADPSQDTEAARRYVK
NVYPEIDEKCLEGFLADAPNLLDFWIANGVEMEKSIGYPDYYMEKEGSARERSVFPGVYKGPKKYRSLVRKTPAYYVPFT
LNEAMTWGVHRFGHWNKTLLTKRAVAGHMTMGKAFIAFLLEACIEAGVDLSLNSTTERLEIENGTVTGAIINGQRITAPV
VMMANGGFSHNPELMKRIDAERPILSVAPEECDTGNGGLGLALEAGLKIGNPYCWWSPVFKTYDDRIEEKPGPDLWAYHS
ALYDRCWPGGIMVDGNGKRFTNESACYNTVGGVLAQGKDPALNNVWLIWGDYYVKNYVRGIVSYLQPAKSYMNKSKTLED
LADKIGIPAANLRETVEHWNSMAGKQKDDDFHRGESPYDNYMGDKFRNGHPNIEKVEGPYQAVRMHAGTLGTKMGPITDE
YGRTQLENGQFVTGLYASGNAAASFFGNFYPGAGATLGQGCVLAYRAARHACGQGD

Sequences:

>Translated_536_residues
MIIKNLPKKCDILIIGSGAAGLTFALAVKKFKPNLHVHVVEKTESVGGCTAYSGGGVWLPGHRFMADPSQDTEAARRYVK
NVYPEIDEKCLEGFLADAPNLLDFWIANGVEMEKSIGYPDYYMEKEGSARERSVFPGVYKGPKKYRSLVRKTPAYYVPFT
LNEAMTWGVHRFGHWNKTLLTKRAVAGHMTMGKAFIAFLLEACIEAGVDLSLNSTTERLEIENGTVTGAIINGQRITAPV
VMMANGGFSHNPELMKRIDAERPILSVAPEECDTGNGGLGLALEAGLKIGNPYCWWSPVFKTYDDRIEEKPGPDLWAYHS
ALYDRCWPGGIMVDGNGKRFTNESACYNTVGGVLAQGKDPALNNVWLIWGDYYVKNYVRGIVSYLQPAKSYMNKSKTLED
LADKIGIPAANLRETVEHWNSMAGKQKDDDFHRGESPYDNYMGDKFRNGHPNIEKVEGPYQAVRMHAGTLGTKMGPITDE
YGRTQLENGQFVTGLYASGNAAASFFGNFYPGAGATLGQGCVLAYRAARHACGQGD
>Mature_536_residues
MIIKNLPKKCDILIIGSGAAGLTFALAVKKFKPNLHVHVVEKTESVGGCTAYSGGGVWLPGHRFMADPSQDTEAARRYVK
NVYPEIDEKCLEGFLADAPNLLDFWIANGVEMEKSIGYPDYYMEKEGSARERSVFPGVYKGPKKYRSLVRKTPAYYVPFT
LNEAMTWGVHRFGHWNKTLLTKRAVAGHMTMGKAFIAFLLEACIEAGVDLSLNSTTERLEIENGTVTGAIINGQRITAPV
VMMANGGFSHNPELMKRIDAERPILSVAPEECDTGNGGLGLALEAGLKIGNPYCWWSPVFKTYDDRIEEKPGPDLWAYHS
ALYDRCWPGGIMVDGNGKRFTNESACYNTVGGVLAQGKDPALNNVWLIWGDYYVKNYVRGIVSYLQPAKSYMNKSKTLED
LADKIGIPAANLRETVEHWNSMAGKQKDDDFHRGESPYDNYMGDKFRNGHPNIEKVEGPYQAVRMHAGTLGTKMGPITDE
YGRTQLENGQFVTGLYASGNAAASFFGNFYPGAGATLGQGCVLAYRAARHACGQGD

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 58974; Mature: 58974

Theoretical pI: Translated: 7.32; Mature: 7.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIKNLPKKCDILIIGSGAAGLTFALAVKKFKPNLHVHVVEKTESVGGCTAYSGGGVWLP
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCEECC
GHRFMADPSQDTEAARRYVKNVYPEIDEKCLEGFLADAPNLLDFWIANGVEMEKSIGYPD
CCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCC
YYMEKEGSARERSVFPGVYKGPKKYRSLVRKTPAYYVPFTLNEAMTWGVHRFGHWNKTLL
CEECCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHH
TKRAVAGHMTMGKAFIAFLLEACIEAGVDLSLNSTTERLEIENGTVTGAIINGQRITAPV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCEEEEEEECCCEECCCE
VMMANGGFSHNPELMKRIDAERPILSVAPEECDTGNGGLGLALEAGLKIGNPYCWWSPVF
EEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
KTYDDRIEEKPGPDLWAYHSALYDRCWPGGIMVDGNGKRFTNESACYNTVGGVLAQGKDP
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCC
ALNNVWLIWGDYYVKNYVRGIVSYLQPAKSYMNKSKTLEDLADKIGIPAANLRETVEHWN
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SMAGKQKDDDFHRGESPYDNYMGDKFRNGHPNIEKVEGPYQAVRMHAGTLGTKMGPITDE
HHCCCCCCCHHHCCCCCHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCH
YGRTQLENGQFVTGLYASGNAAASFFGNFYPGAGATLGQGCVLAYRAARHACGQGD
HCCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIIKNLPKKCDILIIGSGAAGLTFALAVKKFKPNLHVHVVEKTESVGGCTAYSGGGVWLP
CCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEECCCCEECC
GHRFMADPSQDTEAARRYVKNVYPEIDEKCLEGFLADAPNLLDFWIANGVEMEKSIGYPD
CCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCC
YYMEKEGSARERSVFPGVYKGPKKYRSLVRKTPAYYVPFTLNEAMTWGVHRFGHWNKTLL
CEECCCCCCHHHCCCCCCCCCHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCCCCHHHH
TKRAVAGHMTMGKAFIAFLLEACIEAGVDLSLNSTTERLEIENGTVTGAIINGQRITAPV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECCCEEEEEEECCCEECCCE
VMMANGGFSHNPELMKRIDAERPILSVAPEECDTGNGGLGLALEAGLKIGNPYCWWSPVF
EEEECCCCCCCHHHHHHHCCCCCEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHH
KTYDDRIEEKPGPDLWAYHSALYDRCWPGGIMVDGNGKRFTNESACYNTVGGVLAQGKDP
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHCCCCCC
ALNNVWLIWGDYYVKNYVRGIVSYLQPAKSYMNKSKTLEDLADKIGIPAANLRETVEHWN
CCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
SMAGKQKDDDFHRGESPYDNYMGDKFRNGHPNIEKVEGPYQAVRMHAGTLGTKMGPITDE
HHCCCCCCCHHHCCCCCHHHHCCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCCCCCCCCH
YGRTQLENGQFVTGLYASGNAAASFFGNFYPGAGATLGQGCVLAYRAARHACGQGD
HCCCCCCCCCEEEEEEECCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]