BacMap

Definition	Geobacter metallireducens GS-15 chromosome, complete genome.
Accession	NC_007517
Length	3,997,420

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The map label for this gene is paaF [C]

Identifier: 78223429

GI number: 78223429

Start: 2515870

End: 2516652

Strand: Direct

Name: paaF [C]

Synonym: Gmet_2224

Alternate gene names: 78223429

Gene position: 2515870-2516652 (Clockwise)

Preceding gene: 78223427

Following gene: 78223430

Centisome position: 62.94

GC content: 53.51

Gene sequence:

>783_bases
ATGGGATTTCAGGAATTGCTTTACGAGAAAACAGAGGGAGTCGGGGTAATCACCCTGAACAGACCAGATCGGCTGAACGC
TCTCAACCGCACCATCCTGTTGGAGTTGATCCAGGTGTTGCAGGAAGCGACTACCGATAACGAGGTACGGGTTGTACTTA
TCACCGGCGCGGGAAAAGGGTTCTGTGCCGGGGGTGACCTCAAGGGCCATCCGAGTTTTGAAACATCGGACCCGCTGGTT
CGCGAGGGATACGTCAAGGAATCCCATCAGGCGATACTCCTTCTGCACCACATGCCTAAGCCAGTTGTCGCAGCGGTCAA
CGGGGTAGCCGCCGGGGCGGGGATGAATATCGCTCTATCCTGCGACATCCGGCTTGCTTCCGATACCGCTGTCTTTACCG
AATCGTTTATCAAGGCCGGAATCATGACAGATATGGGTGGCAGCTACTTCCTTCCCAGAATCGTCGGTGTGGGACGTGCC
ATTGAAATGATCCTCACTGCCGAGAAGATAGATGCCGCAGAGGCTTGCCGTATAGGTCTGGTCAACAAGGTCTTCCCCGA
CGCAGAATTCAGAACCGCAGCCCTCAGTTATGCGAAGGAACTGGCCAAGGGGCCGAGACAGGCCTACAAAATGGCAAAAT
GGGCCATTTATACAGGATTGCAACTCGACCTGGAGGATGCCTTGAAACACGAAGAACTGGGGCAGGCGCTGCTGATTGGA
ACTGAGGATTCGAGCAATGCAATCAAGGCCTTTATTGAAAAACGAACTCCGGTTTTCAAATAG

Upstream 100 bases:

>100_bases
TAGTTACAGTTTCTGCTCGCACGATCGGTATATGGCACGAAAGCTGCGATAATAAATAGAGTGCAGCAGCATCAAAATAC
AAAAAATGAAAGGATTCATC

Downstream 100 bases:

>100_bases
GAATAAATTCTAAAGATCTCAAAACAACAGGAGAAAATCATGATAATCAAGAACTTACCAAAGAAATGCGATATTTTAAT
CATTGGTTCGGGTGCGGCCG

Product: short chain enoyl-CoA hydratase/enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGFQELLYEKTEGVGVITLNRPDRLNALNRTILLELIQVLQEATTDNEVRVVLITGAGKGFCAGGDLKGHPSFETSDPLV
REGYVKESHQAILLLHHMPKPVVAAVNGVAAGAGMNIALSCDIRLASDTAVFTESFIKAGIMTDMGGSYFLPRIVGVGRA
IEMILTAEKIDAAEACRIGLVNKVFPDAEFRTAALSYAKELAKGPRQAYKMAKWAIYTGLQLDLEDALKHEELGQALLIG
TEDSSNAIKAFIEKRTPVFK

Sequences:

>Translated_260_residues
MGFQELLYEKTEGVGVITLNRPDRLNALNRTILLELIQVLQEATTDNEVRVVLITGAGKGFCAGGDLKGHPSFETSDPLV
REGYVKESHQAILLLHHMPKPVVAAVNGVAAGAGMNIALSCDIRLASDTAVFTESFIKAGIMTDMGGSYFLPRIVGVGRA
IEMILTAEKIDAAEACRIGLVNKVFPDAEFRTAALSYAKELAKGPRQAYKMAKWAIYTGLQLDLEDALKHEELGQALLIG
TEDSSNAIKAFIEKRTPVFK
>Mature_259_residues
GFQELLYEKTEGVGVITLNRPDRLNALNRTILLELIQVLQEATTDNEVRVVLITGAGKGFCAGGDLKGHPSFETSDPLVR
EGYVKESHQAILLLHHMPKPVVAAVNGVAAGAGMNIALSCDIRLASDTAVFTESFIKAGIMTDMGGSYFLPRIVGVGRAI
EMILTAEKIDAAEACRIGLVNKVFPDAEFRTAALSYAKELAKGPRQAYKMAKWAIYTGLQLDLEDALKHEELGQALLIGT
EDSSNAIKAFIEKRTPVFK

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=251, Percent_Identity=34.2629482071713, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI45643119, Length=266, Percent_Identity=30.8270676691729, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI260275230, Length=266, Percent_Identity=30.8270676691729, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI260274832, Length=266, Percent_Identity=30.8270676691729, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI4502327, Length=263, Percent_Identity=34.6007604562738, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=29.8804780876494, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI70995211, Length=182, Percent_Identity=33.5164835164835, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI213417737, Length=230, Percent_Identity=28.695652173913, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI157694516, Length=230, Percent_Identity=28.695652173913, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI37594469, Length=187, Percent_Identity=31.5508021390374, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI37594471, Length=187, Percent_Identity=31.5508021390374, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI4757968, Length=240, Percent_Identity=29.1666666666667, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI49355787, Length=240, Percent_Identity=29.1666666666667, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI25453481, Length=240, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI51538573, Length=240, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI4757966, Length=240, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI51538581, Length=240, Percent_Identity=29.1666666666667, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI116235487, Length=255, Percent_Identity=28.2352941176471, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI68989263, Length=210, Percent_Identity=28.0952380952381, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI221136756, Length=231, Percent_Identity=28.1385281385281, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI221136753, Length=231, Percent_Identity=28.1385281385281, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI221307494, Length=231, Percent_Identity=28.1385281385281, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=30.2857142857143, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694520, Length=205, Percent_Identity=27.3170731707317, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI62530384, Length=222, Percent_Identity=27.9279279279279, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=256, Percent_Identity=37.5, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI1788597, Length=270, Percent_Identity=33.7037037037037, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=35.9375, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI221142681, Length=249, Percent_Identity=34.136546184739, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1788682, Length=195, Percent_Identity=28.7179487179487, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI87082183, Length=263, Percent_Identity=25.8555133079848, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790281, Length=173, Percent_Identity=26.5895953757225, Blast_Score=71, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=248, Percent_Identity=35.4838709677419, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI17540714, Length=231, Percent_Identity=35.0649350649351, Blast_Score=129, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17554946, Length=249, Percent_Identity=32.5301204819277, Blast_Score=120, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17560910, Length=256, Percent_Identity=26.953125, Blast_Score=107, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=254, Percent_Identity=31.496062992126, Blast_Score=103, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17549921, Length=209, Percent_Identity=33.9712918660287, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17540306, Length=240, Percent_Identity=30.8333333333333, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=204, Percent_Identity=30.3921568627451, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=186, Percent_Identity=28.494623655914, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI25144160, Length=193, Percent_Identity=29.5336787564767, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17558304, Length=183, Percent_Identity=30.0546448087432, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25144157, Length=193, Percent_Identity=29.5336787564767, Blast_Score=83, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI25144276, Length=216, Percent_Identity=28.2407407407407, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17508953, Length=216, Percent_Identity=28.2407407407407, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17508951, Length=216, Percent_Identity=28.2407407407407, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=183, Percent_Identity=25.6830601092896, Blast_Score=79, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=36.8421052631579, Blast_Score=144, Evalue=5e-35,
Organism=Drosophila melanogaster, GI20129971, Length=258, Percent_Identity=33.7209302325581, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24653477, Length=258, Percent_Identity=33.7209302325581, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI19920382, Length=256, Percent_Identity=32.03125, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24654903, Length=249, Percent_Identity=28.9156626506024, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21357171, Length=263, Percent_Identity=28.8973384030418, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24650670, Length=208, Percent_Identity=31.25, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19922422, Length=258, Percent_Identity=26.3565891472868, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571729, Length=152, Percent_Identity=30.9210526315789, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28571730, Length=152, Percent_Identity=30.9210526315789, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24583077, Length=182, Percent_Identity=31.3186813186813, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24583079, Length=182, Percent_Identity=31.3186813186813, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI19921000, Length=182, Percent_Identity=31.3186813186813, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28147; Mature: 28016

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFQELLYEKTEGVGVITLNRPDRLNALNRTILLELIQVLQEATTDNEVRVVLITGAGKG
CCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FCAGGDLKGHPSFETSDPLVREGYVKESHQAILLLHHMPKPVVAAVNGVAAGAGMNIALS
EECCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEE
CDIRLASDTAVFTESFIKAGIMTDMGGSYFLPRIVGVGRAIEMILTAEKIDAAEACRIGL
EEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VNKVFPDAEFRTAALSYAKELAKGPRQAYKMAKWAIYTGLQLDLEDALKHEELGQALLIG
HHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEECHHHHHHHHHCCCEEEEE
TEDSSNAIKAFIEKRTPVFK
CCCCHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GFQELLYEKTEGVGVITLNRPDRLNALNRTILLELIQVLQEATTDNEVRVVLITGAGKG
CHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
FCAGGDLKGHPSFETSDPLVREGYVKESHQAILLLHHMPKPVVAAVNGVAAGAGMNIALS
EECCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEEEE
CDIRLASDTAVFTESFIKAGIMTDMGGSYFLPRIVGVGRAIEMILTAEKIDAAEACRIGL
EEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VNKVFPDAEFRTAALSYAKELAKGPRQAYKMAKWAIYTGLQLDLEDALKHEELGQALLIG
HHHHCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCEECHHHHHHHHHCCCEEEEE
TEDSSNAIKAFIEKRTPVFK
CCCCHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA