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The map label for this gene is abfD [H]

Identifier: 78223420

GI number: 78223420

Start: 2506050

End: 2507501

Strand: Reverse

Name: abfD [H]

Synonym: Gmet_2215

Alternate gene names: 78223420

Gene position: 2507501-2506050 (Counterclockwise)

Preceding gene: 78223421

Following gene: 78223419

Centisome position: 62.73

GC content: 54.34

Gene sequence:

>1452_bases
ATGGCACTAAGAGATGGGAATTCCTACCGGGAAAGCCTTCGGGCGCTCAATATCAAAGTCTATGCCTTTGGAGAGAAGAT
TGACAGCATAGTAGATCACCCATTGTTCCAGCCCCATATCAATGCGGCTGCATTGACGTTCGACTTGGCCCATGATCCGA
CCACGGAAGCGCTCGTCACAGCCACCTCACACCTGACGGGGAGTAAAATCAGCCGCTTCACCCATATCCACCAGAGCACC
GACGATCTCATAAAAAAGGTGAAGATGTTGCGGCTTATTGCAGGGAAGACGGGAAGTTGCTACCAGCGCTGTGTGGGGTG
GGATGCCCTGAACGCTAACTATACGGTAACCTATGAGATGGACCAGGAGCTTGGTACCGACTATCACCAGCGTTTTAGGC
GTTACCTCGAATATATACAGGACAATGACCTGATGGTGGCGGGAGCAATGACCGATCCCAAGGGGGACAGGGGGCTGCCT
CCGGCAAAACAGAAAGACCCGGACATGTTCGTGCACGTGGTGGCAAAGAATGACAAGGGGATAGTCATTCGTGGGGCAAA
GGTTCACCAGACCGGAATTGTCAATTCCCATGAAATGCTGATTATGCCAACCATGGCCATGGGGGAGGAGGACGGCGACT
ATGCGGTTGCCTGTGCTCTCCCCACGGATTCCCCCGGTGTCATCCATATCTTTGGTCGTCAAACCAACGATACACGCCGT
CTGGAAAAGGGAGACCTTGATCAGGGTAATGCTGAGTATGGAACTGTCGGAGGCGAGGCTTTGACCATACTTGAAGATGT
CTTCGTCCCGTGGGAACGCGTCTTCATGTGCGGAGAGTACAAGTATGCGGGGCTGCTGGTTGAGCGTTTCGCGAGCTATC
ATCGACAGAACTATGGTGGATGCAAGGCAGGCGTGAGCGATGTGATCATCGGCGCAACTACCGCTATGGCAGAGTACAAC
GGAGCAGCCAAGGCTTCCCACGTGCGTGACAAGATCGTGGAGATGGTCCACCTCACCGAGACCCTTTATTGCGGTTCCAT
CGCCTGCTCCTGTGAGGGTGCTCCCACGCCGTCAGGGGCCTATTTCGTCAATCCCCTGCTGGCCAATACGGTTAAGCAGA
ACGTGACCCGTTTCATCTATGAGATTGCACGCCTTTCCCACGATATTTCCGGTGGCTGCATGGCAACCATGCCTTCGGAG
AAGGATCTGCACCACGATGAGATCGGCAAATATGTAGAGAAGTATTTCCGGGGGGTGGACGAAGCTCCCACTGAAGAGCG
CATGCGGATGGCCCGGCTCGTTGAAAATATGACGGGCGGCACGGCTTTGGTGGAAAGCATGCATGGTGCCGGCTCTCCCC
AGGCGCAGAGAGTCATGATCCTCCGCCAGGCAAATCTCGGCCATAAGGTAAAGCTTGCCAAGAAACTGGCCGGCATAAAG
GAAGAAAAATAG

Upstream 100 bases:

>100_bases
CGTTCGGTATATGATGGCCAGTCGATGTGTGGACTCGTAGCAGCGGTAGACAGAAAATCTTATTTTTCAAATACTCGGGA
ACATTTGGGGGGAGGTAAAG

Downstream 100 bases:

>100_bases
CGGGGCCATGGGGCATTTCAGGGTCTGTGTCAAGTATTGCGGCAACTGCAACCCTGAGGTGGATACGCCCGGGATTGTCA
GGAAGGTACAGGCGCTTGCC

Product: vinylacetyl-CoA delta-isomerase/4-hydroxybutyryl-CoA dehydratase

Products: NA

Alternate protein names: 4-BUDH; Gamma-aminobutyrate metabolism dehydratase/isomerase [H]

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MALRDGNSYRESLRALNIKVYAFGEKIDSIVDHPLFQPHINAAALTFDLAHDPTTEALVTATSHLTGSKISRFTHIHQST
DDLIKKVKMLRLIAGKTGSCYQRCVGWDALNANYTVTYEMDQELGTDYHQRFRRYLEYIQDNDLMVAGAMTDPKGDRGLP
PAKQKDPDMFVHVVAKNDKGIVIRGAKVHQTGIVNSHEMLIMPTMAMGEEDGDYAVACALPTDSPGVIHIFGRQTNDTRR
LEKGDLDQGNAEYGTVGGEALTILEDVFVPWERVFMCGEYKYAGLLVERFASYHRQNYGGCKAGVSDVIIGATTAMAEYN
GAAKASHVRDKIVEMVHLTETLYCGSIACSCEGAPTPSGAYFVNPLLANTVKQNVTRFIYEIARLSHDISGGCMATMPSE
KDLHHDEIGKYVEKYFRGVDEAPTEERMRMARLVENMTGGTALVESMHGAGSPQAQRVMILRQANLGHKVKLAKKLAGIK
EEK

Sequences:

>Translated_483_residues
MALRDGNSYRESLRALNIKVYAFGEKIDSIVDHPLFQPHINAAALTFDLAHDPTTEALVTATSHLTGSKISRFTHIHQST
DDLIKKVKMLRLIAGKTGSCYQRCVGWDALNANYTVTYEMDQELGTDYHQRFRRYLEYIQDNDLMVAGAMTDPKGDRGLP
PAKQKDPDMFVHVVAKNDKGIVIRGAKVHQTGIVNSHEMLIMPTMAMGEEDGDYAVACALPTDSPGVIHIFGRQTNDTRR
LEKGDLDQGNAEYGTVGGEALTILEDVFVPWERVFMCGEYKYAGLLVERFASYHRQNYGGCKAGVSDVIIGATTAMAEYN
GAAKASHVRDKIVEMVHLTETLYCGSIACSCEGAPTPSGAYFVNPLLANTVKQNVTRFIYEIARLSHDISGGCMATMPSE
KDLHHDEIGKYVEKYFRGVDEAPTEERMRMARLVENMTGGTALVESMHGAGSPQAQRVMILRQANLGHKVKLAKKLAGIK
EEK
>Mature_482_residues
ALRDGNSYRESLRALNIKVYAFGEKIDSIVDHPLFQPHINAAALTFDLAHDPTTEALVTATSHLTGSKISRFTHIHQSTD
DLIKKVKMLRLIAGKTGSCYQRCVGWDALNANYTVTYEMDQELGTDYHQRFRRYLEYIQDNDLMVAGAMTDPKGDRGLPP
AKQKDPDMFVHVVAKNDKGIVIRGAKVHQTGIVNSHEMLIMPTMAMGEEDGDYAVACALPTDSPGVIHIFGRQTNDTRRL
EKGDLDQGNAEYGTVGGEALTILEDVFVPWERVFMCGEYKYAGLLVERFASYHRQNYGGCKAGVSDVIIGATTAMAEYNG
AAKASHVRDKIVEMVHLTETLYCGSIACSCEGAPTPSGAYFVNPLLANTVKQNVTRFIYEIARLSHDISGGCMATMPSEK
DLHHDEIGKYVEKYFRGVDEAPTEERMRMARLVENMTGGTALVESMHGAGSPQAQRVMILRQANLGHKVKLAKKLAGIKE
EK

Specific function: Catalyzes the reversible conversion of 4-hydroxybutyryl- CoA to crotonyl-CoA. The mechanism of the reaction seems to go through three steps:(1) the FAD-dependent oxidation of 4- hydroxybutyryl-CoA to 4-hydroxycrotonyl-CoA; (2) the hydroxyl group is substi

COG id: COG2368

COG function: function code Q; Aromatic ring hydroxylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR009100
- InterPro:   IPR004925 [H]

Pfam domain/function: PF03241 HpaB [H]

EC number: =4.2.1.120; =5.3.3.3 [H]

Molecular weight: Translated: 53359; Mature: 53227

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRDGNSYRESLRALNIKVYAFGEKIDSIVDHPLFQPHINAAALTFDLAHDPTTEALVT
CCCCCCHHHHHHHHHEEEEEEEECHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHH
ATSHLTGSKISRFTHIHQSTDDLIKKVKMLRLIAGKTGSCYQRCVGWDALNANYTVTYEM
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEC
DQELGTDYHQRFRRYLEYIQDNDLMVAGAMTDPKGDRGLPPAKQKDPDMFVHVVAKNDKG
CHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCE
IVIRGAKVHQTGIVNSHEMLIMPTMAMGEEDGDYAVACALPTDSPGVIHIFGRQTNDTRR
EEEECCEEEECCCCCCCCEEEEEHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHH
LEKGDLDQGNAEYGTVGGEALTILEDVFVPWERVFMCGEYKYAGLLVERFASYHRQNYGG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHEEECCCHHHHHHHHHHHHHHHCCCCC
CKAGVSDVIIGATTAMAEYNGAAKASHVRDKIVEMVHLTETLYCGSIACSCEGAPTPSGA
CCCCHHHHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
YFVNPLLANTVKQNVTRFIYEIARLSHDISGGCMATMPSEKDLHHDEIGKYVEKYFRGVD
EEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCC
EAPTEERMRMARLVENMTGGTALVESMHGAGSPQAQRVMILRQANLGHKVKLAKKLAGIK
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHEEEEEEECCCCCHHHHHHHHCCCC
EEK
CCC
>Mature Secondary Structure 
ALRDGNSYRESLRALNIKVYAFGEKIDSIVDHPLFQPHINAAALTFDLAHDPTTEALVT
CCCCCHHHHHHHHHEEEEEEEECHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCHHHHH
ATSHLTGSKISRFTHIHQSTDDLIKKVKMLRLIAGKTGSCYQRCVGWDALNANYTVTYEM
HHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCEEEEEEC
DQELGTDYHQRFRRYLEYIQDNDLMVAGAMTDPKGDRGLPPAKQKDPDMFVHVVAKNDKG
CHHHCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCE
IVIRGAKVHQTGIVNSHEMLIMPTMAMGEEDGDYAVACALPTDSPGVIHIFGRQTNDTRR
EEEECCEEEECCCCCCCCEEEEEHHHCCCCCCCEEEEEECCCCCCCEEEEECCCCCCHHH
LEKGDLDQGNAEYGTVGGEALTILEDVFVPWERVFMCGEYKYAGLLVERFASYHRQNYGG
CCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHEEECCCHHHHHHHHHHHHHHHCCCCC
CKAGVSDVIIGATTAMAEYNGAAKASHVRDKIVEMVHLTETLYCGSIACSCEGAPTPSGA
CCCCHHHHHEEHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCC
YFVNPLLANTVKQNVTRFIYEIARLSHDISGGCMATMPSEKDLHHDEIGKYVEKYFRGVD
EEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCC
EAPTEERMRMARLVENMTGGTALVESMHGAGSPQAQRVMILRQANLGHKVKLAKKLAGIK
CCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHEEEEEEECCCCCHHHHHHHHCCCC
EEK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11041350; 8344309 [H]