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The map label for this gene is fadH [C]

Identifier: 78223407

GI number: 78223407

Start: 2490779

End: 2492845

Strand: Reverse

Name: fadH [C]

Synonym: Gmet_2202

Alternate gene names: 78223407

Gene position: 2492845-2490779 (Counterclockwise)

Preceding gene: 78223408

Following gene: 78223406

Centisome position: 62.36

GC content: 58.68

Gene sequence:

>2067_bases
ATGAAGCTTTTTGAACCGCTTACAATACGCGGCATGACCGTAAAGAACAGAATCGTCATGTCATCCATGGGGGTAGGGCT
CGGTTATTCAAACAAGCGGGTAATCGATTTCTACGTGGAACGAGCTAAAGGAGGCGTTGGCACTATCATCGTCGGTGCAG
GAATTCCGGACCTGTTCATGAATAATGATGTATGGGACAAGCCGGGCGGCGTAGAGACCTTCATCAAGCGCCTGAAGTCG
CTTACGGCTGCGGTGAATGATGCTGGTGCCAAGATTGGCATCCAGTTCATCTACGGTAACCGTTATCCTCTCGGTCTGGA
TTTGACCAGCGGCGAGCTAGCCGCCCCTTCCGCCAGGGTTGAGCCCAATCCATCCCGTCACGCCTTTGTAAAACCGGGCG
AGCAGTTACGCGAACTTACCTTGACGGAAATCTCGTCCATCATCGACAAGATCGCCAAGGCCGCGGCCGGCGCCAGGGAA
GCAGGATTCAATTTCGTGGAGCTGCACAATGGACACGGCATGCTCCATTGCCAGTTTTTTTCTCCTACCACCAATCAGCG
CACGGACCAGTATGGCGGCGACCCGCAGAGACGCATGGCATTCGGGATTGAAACCGTGCGCGCCATGCGTAATGCAGTAG
GTGAAGATTTCCCCCTCTTTGTCCGACACGGTGTGGTAGATGCGGTGCCGGACGGAGCAAGCCTGGCAGAAGGCGTAGCC
TTTGCCGTTGAGTTGGAGAAGGCCGGTGCCGATGTGCTGAACGTGTCTCTGGCTACCCCCCCTATTTGCGGCGGCTATGT
GCCGACCGGCGCTGACCCGGTGGCGACCCATGTCCATCTCGCAGAGGCGGTTAAACGGGCTGTCGGCATCCCGGTTATCG
GGGTCGGGAGGATCAAGACCCCTGACGTTGCCGAATCCATTCTGGCCCAGGGAAAGGTTGACCTGGTTGCCATCGGTCGC
CAGCTATTCGCCGATCCCCACTGGCCGCTGAAAGCAGCCGAAGGACGTATCGCCGAGATCGTGCCGTGCATTGACTGCCA
TGAGTGTTATGAGCGGGCAACCGCGGGGGAGGGTGTGGAGTGTACGGTCAACTATGCTGCCGGCCGGGAAGACGAAAGGA
GGATCAGGCCCGCTGAAAGGAAAAGGCGGGTTCTTGTGGTGGGGGGGGGGCCGGCAGGTATGGAAGCTGCCCGGATTGCA
GCCATGCGGGGGCATGATGTGACGTTGCAGGAAAGGAGCAGACAGTTGGGCGGCGCCATGCTGTTACAGGCCATGGTTCC
CTCAAAGGCGGAAGTGGAAAAACTGACGGCGTGTCAAACCGCCCAGGTCTCAAATGAAGGGGTTCGCCTCATGCTCGGGC
AGCCGGCCGGGCCTGGGCATTTGGGTATCGATGGGACGGATGTCGTTGTTGTCGCCATAGGAGCGAAACCGATCAAACCT
GACATTCCGGGTATAAACAGGGAGAATGTCGTCAGCAGTGGTGCCATTCGCCTCATGATGTCCGGTATTTCCGGCCCTGG
CGCCGGGACGATCCGGACCGGTTGGCGCGGCCAACTCATCCGGTTTGGCGGGAGTTTTCTCGACAGACCCCTGTCGCTCT
CCCTGCGCAAGCGGCTGGCGGGACTCGGCATTCCCGTCATGTTCGGCAAGAGTGTTGCCATCATGGGGGGGGGGATGATG
GCCTGCCAACTTGCGGATATGCTGGCTGAACATGGCCGGGAAGTGGCCATCGTGACGCAGGATGAAGAATTGGCTGCGGA
TATGGTCTCCACCCTCAGATTCAAGCTGATGAACAGGCTGTCACAACAGCGTGTGACCATTGTCAGGGGTATTGCCGGAT
TTGAGGAAGTGACGGAGCGTGGTCTGGTCATCCGTGATGCCGGTGGAAACAGGATGACGATCCCTGCCGCTACCGTCGTA
CCGATGCTCGGTTTCACGAATGACAGTGAAGCGGTAGAGGCGCTGCGCCGGCAGATACCGGAGCTCTTTGCAGCCGGTGA
TTGCTCCGAGCCGCTCAACCTCCTCCATGCCATTCATGACGGCGGCAGGATCGGACGAGAGATCTAG

Upstream 100 bases:

>100_bases
TGAATCTGGCGGACAGGTGAAAAACGGCGCTACCGTCTTGCACGGTACATCATGTAAATAAAGGACAAATCTCGAAAAAG
GTCTGCAGGAGGATGGTGAC

Downstream 100 bases:

>100_bases
GGCGGGGTAGCCCTGTTACCCCGTAAAGGAAATACCGTGCTGCAGATGCGTACAGACCTGATGGCGGTGGCGTGCGGTCG
GCGCTCCGCCGATCTCTATA

Product: 2,4-dienoyl-CoA reductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MKLFEPLTIRGMTVKNRIVMSSMGVGLGYSNKRVIDFYVERAKGGVGTIIVGAGIPDLFMNNDVWDKPGGVETFIKRLKS
LTAAVNDAGAKIGIQFIYGNRYPLGLDLTSGELAAPSARVEPNPSRHAFVKPGEQLRELTLTEISSIIDKIAKAAAGARE
AGFNFVELHNGHGMLHCQFFSPTTNQRTDQYGGDPQRRMAFGIETVRAMRNAVGEDFPLFVRHGVVDAVPDGASLAEGVA
FAVELEKAGADVLNVSLATPPICGGYVPTGADPVATHVHLAEAVKRAVGIPVIGVGRIKTPDVAESILAQGKVDLVAIGR
QLFADPHWPLKAAEGRIAEIVPCIDCHECYERATAGEGVECTVNYAAGREDERRIRPAERKRRVLVVGGGPAGMEAARIA
AMRGHDVTLQERSRQLGGAMLLQAMVPSKAEVEKLTACQTAQVSNEGVRLMLGQPAGPGHLGIDGTDVVVVAIGAKPIKP
DIPGINRENVVSSGAIRLMMSGISGPGAGTIRTGWRGQLIRFGGSFLDRPLSLSLRKRLAGLGIPVMFGKSVAIMGGGMM
ACQLADMLAEHGREVAIVTQDEELAADMVSTLRFKLMNRLSQQRVTIVRGIAGFEEVTERGLVIRDAGGNRMTIPAATVV
PMLGFTNDSEAVEALRRQIPELFAAGDCSEPLNLLHAIHDGGRIGREI

Sequences:

>Translated_688_residues
MKLFEPLTIRGMTVKNRIVMSSMGVGLGYSNKRVIDFYVERAKGGVGTIIVGAGIPDLFMNNDVWDKPGGVETFIKRLKS
LTAAVNDAGAKIGIQFIYGNRYPLGLDLTSGELAAPSARVEPNPSRHAFVKPGEQLRELTLTEISSIIDKIAKAAAGARE
AGFNFVELHNGHGMLHCQFFSPTTNQRTDQYGGDPQRRMAFGIETVRAMRNAVGEDFPLFVRHGVVDAVPDGASLAEGVA
FAVELEKAGADVLNVSLATPPICGGYVPTGADPVATHVHLAEAVKRAVGIPVIGVGRIKTPDVAESILAQGKVDLVAIGR
QLFADPHWPLKAAEGRIAEIVPCIDCHECYERATAGEGVECTVNYAAGREDERRIRPAERKRRVLVVGGGPAGMEAARIA
AMRGHDVTLQERSRQLGGAMLLQAMVPSKAEVEKLTACQTAQVSNEGVRLMLGQPAGPGHLGIDGTDVVVVAIGAKPIKP
DIPGINRENVVSSGAIRLMMSGISGPGAGTIRTGWRGQLIRFGGSFLDRPLSLSLRKRLAGLGIPVMFGKSVAIMGGGMM
ACQLADMLAEHGREVAIVTQDEELAADMVSTLRFKLMNRLSQQRVTIVRGIAGFEEVTERGLVIRDAGGNRMTIPAATVV
PMLGFTNDSEAVEALRRQIPELFAAGDCSEPLNLLHAIHDGGRIGREI
>Mature_688_residues
MKLFEPLTIRGMTVKNRIVMSSMGVGLGYSNKRVIDFYVERAKGGVGTIIVGAGIPDLFMNNDVWDKPGGVETFIKRLKS
LTAAVNDAGAKIGIQFIYGNRYPLGLDLTSGELAAPSARVEPNPSRHAFVKPGEQLRELTLTEISSIIDKIAKAAAGARE
AGFNFVELHNGHGMLHCQFFSPTTNQRTDQYGGDPQRRMAFGIETVRAMRNAVGEDFPLFVRHGVVDAVPDGASLAEGVA
FAVELEKAGADVLNVSLATPPICGGYVPTGADPVATHVHLAEAVKRAVGIPVIGVGRIKTPDVAESILAQGKVDLVAIGR
QLFADPHWPLKAAEGRIAEIVPCIDCHECYERATAGEGVECTVNYAAGREDERRIRPAERKRRVLVVGGGPAGMEAARIA
AMRGHDVTLQERSRQLGGAMLLQAMVPSKAEVEKLTACQTAQVSNEGVRLMLGQPAGPGHLGIDGTDVVVVAIGAKPIKP
DIPGINRENVVSSGAIRLMMSGISGPGAGTIRTGWRGQLIRFGGSFLDRPLSLSLRKRLAGLGIPVMFGKSVAIMGGGMM
ACQLADMLAEHGREVAIVTQDEELAADMVSTLRFKLMNRLSQQRVTIVRGIAGFEEVTERGLVIRDAGGNRMTIPAATVV
PMLGFTNDSEAVEALRRQIPELFAAGDCSEPLNLLHAIHDGGRIGREI

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=505, Percent_Identity=35.2475247524752, Blast_Score=252, Evalue=4e-68,
Organism=Escherichia coli, GI1787939, Length=361, Percent_Identity=29.3628808864266, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17559802, Length=236, Percent_Identity=29.2372881355932, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17564188, Length=202, Percent_Identity=26.7326732673267, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17540738, Length=209, Percent_Identity=26.7942583732057, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI72001454, Length=210, Percent_Identity=27.6190476190476, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17565138, Length=326, Percent_Identity=24.8466257668712, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17559804, Length=206, Percent_Identity=25.7281553398058, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6321973, Length=286, Percent_Identity=26.2237762237762, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 73290; Mature: 73290

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFEPLTIRGMTVKNRIVMSSMGVGLGYSNKRVIDFYVERAKGGVGTIIVGAGIPDLFM
CCCCCCCEEECEEEHHEEEEHHCCCCCCCCCCEEEEEEHHHCCCCCEEEEEECCCCHHHC
NNDVWDKPGGVETFIKRLKSLTAAVNDAGAKIGIQFIYGNRYPLGLDLTSGELAAPSARV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCC
EPNPSRHAFVKPGEQLRELTLTEISSIIDKIAKAAAGAREAGFNFVELHNGHGMLHCQFF
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCEEEEEEE
SPTTNQRTDQYGGDPQRRMAFGIETVRAMRNAVGEDFPLFVRHGVVDAVPDGASLAEGVA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHCCCCCHHHHCCEE
FAVELEKAGADVLNVSLATPPICGGYVPTGADPVATHVHLAEAVKRAVGIPVIGVGRIKT
EEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCC
PDVAESILAQGKVDLVAIGRQLFADPHWPLKAAEGRIAEIVPCIDCHECYERATAGEGVE
CHHHHHHHHCCCEEHEEHHHHHHCCCCCCCCCCCCCCEEHCCCCCHHHHHHHHCCCCCCE
CTVNYAAGREDERRIRPAERKRRVLVVGGGPAGMEAARIAAMRGHDVTLQERSRQLGGAM
EEEEECCCCCHHHHCCCHHHCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
LLQAMVPSKAEVEKLTACQTAQVSNEGVRLMLGQPAGPGHLGIDGTDVVVVAIGAKPIKP
HHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCC
DIPGINRENVVSSGAIRLMMSGISGPGAGTIRTGWRGQLIRFGGSFLDRPLSLSLRKRLA
CCCCCCHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCHHEECCCHHHCCCCHHHHHHHHH
GLGIPVMFGKSVAIMGGGMMACQLADMLAEHGREVAIVTQDEELAADMVSTLRFKLMNRL
HCCCEEHCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
SQQRVTIVRGIAGFEEVTERGLVIRDAGGNRMTIPAATVVPMLGFTNDSEAVEALRRQIP
HHHHHHHHHHHHCHHHHHHCCEEEEECCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHH
ELFAAGDCSEPLNLLHAIHDGGRIGREI
HHHHCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKLFEPLTIRGMTVKNRIVMSSMGVGLGYSNKRVIDFYVERAKGGVGTIIVGAGIPDLFM
CCCCCCCEEECEEEHHEEEEHHCCCCCCCCCCEEEEEEHHHCCCCCEEEEEECCCCHHHC
NNDVWDKPGGVETFIKRLKSLTAAVNDAGAKIGIQFIYGNRYPLGLDLTSGELAAPSARV
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCC
EPNPSRHAFVKPGEQLRELTLTEISSIIDKIAKAAAGAREAGFNFVELHNGHGMLHCQFF
CCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCCEEEEEEE
SPTTNQRTDQYGGDPQRRMAFGIETVRAMRNAVGEDFPLFVRHGVVDAVPDGASLAEGVA
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCHHCCCCCHHHHCCEE
FAVELEKAGADVLNVSLATPPICGGYVPTGADPVATHVHLAEAVKRAVGIPVIGVGRIKT
EEEEECCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCC
PDVAESILAQGKVDLVAIGRQLFADPHWPLKAAEGRIAEIVPCIDCHECYERATAGEGVE
CHHHHHHHHCCCEEHEEHHHHHHCCCCCCCCCCCCCCEEHCCCCCHHHHHHHHCCCCCCE
CTVNYAAGREDERRIRPAERKRRVLVVGGGPAGMEAARIAAMRGHDVTLQERSRQLGGAM
EEEEECCCCCHHHHCCCHHHCCEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHH
LLQAMVPSKAEVEKLTACQTAQVSNEGVRLMLGQPAGPGHLGIDGTDVVVVAIGAKPIKP
HHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCC
DIPGINRENVVSSGAIRLMMSGISGPGAGTIRTGWRGQLIRFGGSFLDRPLSLSLRKRLA
CCCCCCHHHHHCCCHHEEEEECCCCCCCCCCCCCCCCHHEECCCHHHCCCCHHHHHHHHH
GLGIPVMFGKSVAIMGGGMMACQLADMLAEHGREVAIVTQDEELAADMVSTLRFKLMNRL
HCCCEEHCCCEEEEECCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHHH
SQQRVTIVRGIAGFEEVTERGLVIRDAGGNRMTIPAATVVPMLGFTNDSEAVEALRRQIP
HHHHHHHHHHHHCHHHHHHCCEEEEECCCCEEEECHHHHHHHHCCCCCHHHHHHHHHHHH
ELFAAGDCSEPLNLLHAIHDGGRIGREI
HHHHCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]