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The map label for this gene is fadA [H]

Identifier: 78223402

GI number: 78223402

Start: 2484408

End: 2485616

Strand: Reverse

Name: fadA [H]

Synonym: Gmet_2197

Alternate gene names: 78223402

Gene position: 2485616-2484408 (Counterclockwise)

Preceding gene: 78223403

Following gene: 78223401

Centisome position: 62.18

GC content: 57.07

Gene sequence:

>1209_bases
ATGAAAAATGAAGCATACATCTATGACGCGATACGTACGCCGCGAGGCAAGGGAAAGAGTGATGGATCGCTGCACGAGGT
AAAGCCGGTTACCTTGCTGGGTGACCTCTTGAATGAGTTGAAGGTGCGTAACAATCTGGACACATCTCAGGTGGAAGACA
TCGTCATTGGGTGCGTCACCCCCCTGGGTGATCAGGGGGGGAATATCGCCAAAACCGCGGCGTTGTATGCCGGATGGGAC
GATGTGGTTGCCGGCTTCCAGTTGAACCGGTTCTGCGCTTCGGGATTGGAGGCGGTGAACCTGGCGGCGATGAAGGTGAT
ATCCGGATGGGAAGATCTTGTGGTGGCGGGTGGCGTGGAATCCATGTCGCGGGTAACCATCAGTTCCGATGGAGGTGCCC
TTGGCCTGGACCCGGAAACGAGCCTTAAGACCGGCTTTATCCCTCAAGGGGTATCTGCGGATCTGATAGCTACACTGGAG
GGATTTTCCCGCGAAGATGTGGATGCCTTTGCGCTGGAGTCCCAGAAGAAGGCGGCGGAGGCTTCCGAGAAAGGCCATTT
CTCTAAATCGCTGGTTCCGGTGAAGGACAGAAACGGCTTGACGATCCTTGGCCACGACGAGTTCATCCGCAAAAGTACGA
CCATGGAAGGTCTGGCCCAACTGAAACCCTCCTTCGAGGGGGTTGGCGCCGTCGGTTTCGATACGGTTGCGTTACGCAAA
TATACGCAACTCAAGGGGATCAACCATGTCCATACGCCGGGGAACTCGTCGGGTATTGTTGATGGAGCCGCTGCGGTGCT
GGTAGGCAGTTCCGCCAAGGGGAAGGAGTTGGGACTCACGCCGCGTGGACGCGTGGTGGCCGGGGTGATCACCAGTTGCG
AACCGACAATTATGCTCACCGGTCCGACGGCAGCTACTCGCAAGGTACTGCAGAAAGCGGGGCTGAAGATCAGCGACATC
GATCTGTTCGAAGTTAATGAAGCCTTCGCCGCAGTCGTCATGAAATTCCGGAAAGACCTGGACATACCGGCAGAAAAGAT
CAACGTGAACGGCGGGGCAATCGCGATGGGACATCCCCTTGGGGCCAGTGGTGCCATGATATTAGGAACCGTTCTGGATG
AACTGGAGCGGCGCAGGCTGCGCTATGGCCTGTGCACCCTCTGCGTCGGTGGCGGAATCGGGATAGCGACCATTGTCGAA
CGGCTGTGA

Upstream 100 bases:

>100_bases
GTCGTGCAAAGGCCGCGGAGCAGGCACTCGGAGATGCGACCTACCATCGGGAAATCGTAGCGCAGTTGATGGGACTCTAA
TTTAGCAAGAGGGGATCTGT

Downstream 100 bases:

>100_bases
CAGCGGCACACAATCAAACAATAAAAGGTGGGACATATATGAGCGCATACAGATATGAAAGGGATGGAGAGGGTATCGTA
ACCGTTACCATCAATCCCGC

Product: acetyl-CoA acetyltransferase

Products: CoA; 3-oxoacyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKNEAYIYDAIRTPRGKGKSDGSLHEVKPVTLLGDLLNELKVRNNLDTSQVEDIVIGCVTPLGDQGGNIAKTAALYAGWD
DVVAGFQLNRFCASGLEAVNLAAMKVISGWEDLVVAGGVESMSRVTISSDGGALGLDPETSLKTGFIPQGVSADLIATLE
GFSREDVDAFALESQKKAAEASEKGHFSKSLVPVKDRNGLTILGHDEFIRKSTTMEGLAQLKPSFEGVGAVGFDTVALRK
YTQLKGINHVHTPGNSSGIVDGAAAVLVGSSAKGKELGLTPRGRVVAGVITSCEPTIMLTGPTAATRKVLQKAGLKISDI
DLFEVNEAFAAVVMKFRKDLDIPAEKINVNGGAIAMGHPLGASGAMILGTVLDELERRRLRYGLCTLCVGGGIGIATIVE
RL

Sequences:

>Translated_402_residues
MKNEAYIYDAIRTPRGKGKSDGSLHEVKPVTLLGDLLNELKVRNNLDTSQVEDIVIGCVTPLGDQGGNIAKTAALYAGWD
DVVAGFQLNRFCASGLEAVNLAAMKVISGWEDLVVAGGVESMSRVTISSDGGALGLDPETSLKTGFIPQGVSADLIATLE
GFSREDVDAFALESQKKAAEASEKGHFSKSLVPVKDRNGLTILGHDEFIRKSTTMEGLAQLKPSFEGVGAVGFDTVALRK
YTQLKGINHVHTPGNSSGIVDGAAAVLVGSSAKGKELGLTPRGRVVAGVITSCEPTIMLTGPTAATRKVLQKAGLKISDI
DLFEVNEAFAAVVMKFRKDLDIPAEKINVNGGAIAMGHPLGASGAMILGTVLDELERRRLRYGLCTLCVGGGIGIATIVE
RL
>Mature_402_residues
MKNEAYIYDAIRTPRGKGKSDGSLHEVKPVTLLGDLLNELKVRNNLDTSQVEDIVIGCVTPLGDQGGNIAKTAALYAGWD
DVVAGFQLNRFCASGLEAVNLAAMKVISGWEDLVVAGGVESMSRVTISSDGGALGLDPETSLKTGFIPQGVSADLIATLE
GFSREDVDAFALESQKKAAEASEKGHFSKSLVPVKDRNGLTILGHDEFIRKSTTMEGLAQLKPSFEGVGAVGFDTVALRK
YTQLKGINHVHTPGNSSGIVDGAAAVLVGSSAKGKELGLTPRGRVVAGVITSCEPTIMLTGPTAATRKVLQKAGLKISDI
DLFEVNEAFAAVVMKFRKDLDIPAEKINVNGGAIAMGHPLGASGAMILGTVLDELERRRLRYGLCTLCVGGGIGIATIVE
RL

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4501853, Length=389, Percent_Identity=38.0462724935733, Blast_Score=222, Evalue=6e-58,
Organism=Homo sapiens, GI148539872, Length=400, Percent_Identity=38, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI167614485, Length=364, Percent_Identity=34.3406593406593, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI4557237, Length=411, Percent_Identity=32.1167883211679, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI4504327, Length=443, Percent_Identity=31.1512415349887, Blast_Score=148, Evalue=1e-35,
Organism=Homo sapiens, GI194353979, Length=89, Percent_Identity=49.438202247191, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1787663, Length=421, Percent_Identity=40.3800475059382, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI48994986, Length=407, Percent_Identity=40.2948402948403, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1788554, Length=417, Percent_Identity=38.3693045563549, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI87082165, Length=415, Percent_Identity=36.6265060240964, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI1788683, Length=426, Percent_Identity=26.5258215962441, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI133906874, Length=372, Percent_Identity=38.7096774193548, Blast_Score=219, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI25147385, Length=419, Percent_Identity=31.5035799522673, Blast_Score=170, Evalue=9e-43,
Organism=Caenorhabditis elegans, GI17535921, Length=414, Percent_Identity=31.6425120772947, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI17551802, Length=441, Percent_Identity=27.891156462585, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17535917, Length=404, Percent_Identity=26.7326732673267, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6322031, Length=404, Percent_Identity=34.6534653465347, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6325229, Length=420, Percent_Identity=30.4761904761905, Blast_Score=143, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24655093, Length=415, Percent_Identity=38.5542168674699, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI17648125, Length=414, Percent_Identity=34.2995169082126, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24640423, Length=420, Percent_Identity=30.4761904761905, Blast_Score=161, Evalue=6e-40,
Organism=Drosophila melanogaster, GI17137578, Length=444, Percent_Identity=30.1801801801802, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: 2.3.1.16

Molecular weight: Translated: 42173; Mature: 42173

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEAYIYDAIRTPRGKGKSDGSLHEVKPVTLLGDLLNELKVRNNLDTSQVEDIVIGCVT
CCCCEEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEEC
PLGDQGGNIAKTAALYAGWDDVVAGFQLNRFCASGLEAVNLAAMKVISGWEDLVVAGGVE
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHEEEECCCC
SMSRVTISSDGGALGLDPETSLKTGFIPQGVSADLIATLEGFSREDVDAFALESQKKAAE
HHEEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ASEKGHFSKSLVPVKDRNGLTILGHDEFIRKSTTMEGLAQLKPSFEGVGAVGFDTVALRK
HHHCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
YTQLKGINHVHTPGNSSGIVDGAAAVLVGSSAKGKELGLTPRGRVVAGVITSCEPTIMLT
HHHHCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEE
GPTAATRKVLQKAGLKISDIDLFEVNEAFAAVVMKFRKDLDIPAEKINVNGGAIAMGHPL
CCCHHHHHHHHHCCCEEECCEEEHHHHHHHHHHHHHHHCCCCCHHHEECCCCEEEECCCC
GASGAMILGTVLDELERRRLRYGLCTLCVGGGIGIATIVERL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC
>Mature Secondary Structure
MKNEAYIYDAIRTPRGKGKSDGSLHEVKPVTLLGDLLNELKVRNNLDTSQVEDIVIGCVT
CCCCEEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEEC
PLGDQGGNIAKTAALYAGWDDVVAGFQLNRFCASGLEAVNLAAMKVISGWEDLVVAGGVE
CCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHEEEECCCC
SMSRVTISSDGGALGLDPETSLKTGFIPQGVSADLIATLEGFSREDVDAFALESQKKAAE
HHEEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHH
ASEKGHFSKSLVPVKDRNGLTILGHDEFIRKSTTMEGLAQLKPSFEGVGAVGFDTVALRK
HHHCCCCCCCCCEEECCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
YTQLKGINHVHTPGNSSGIVDGAAAVLVGSSAKGKELGLTPRGRVVAGVITSCEPTIMLT
HHHHCCCCEECCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCEEEEE
GPTAATRKVLQKAGLKISDIDLFEVNEAFAAVVMKFRKDLDIPAEKINVNGGAIAMGHPL
CCCHHHHHHHHHCCCEEECCEEEHHHHHHHHHHHHHHHCCCCCHHHEECCCCEEEECCCC
GASGAMILGTVLDELERRRLRYGLCTLCVGGGIGIATIVERL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acetyl-CoA

Specific reaction: acyl-CoA + acetyl-CoA = CoA + 3-oxoacyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]