Click here to switch to the map view.

The map label for this gene is cotA [H]

Identifier: 78223362

GI number: 78223362

Start: 2426431

End: 2428557

Strand: Reverse

Name: cotA [H]

Synonym: Gmet_2155

Alternate gene names: 78223362

Gene position: 2428557-2426431 (Counterclockwise)

Preceding gene: 78223363

Following gene: 78223361

Centisome position: 60.75

GC content: 63.75

Gene sequence:

>2127_bases
ATGGACGGACGATCATTGTGGAGAGGAGCAGTTGCAGCCGGCATTACCCTGGCGCTCGGCATCGGGAACGCCTCCGCCAG
CTTCCTTGTCCCCCAGACGGCGCTGCCGGGGAAGAATGTCCCCAAGTATCGGGATCCGCTTCCCACCTTCGCGGGATCAC
GGGTGCCGGCCACGTCGGGGTACACGGTTTCCTTCGAGGAATTCAAACAGCAGGTCCTCCCGGCGTCGGGCGGCTTTGGC
CCAACGACCGTCTGGGGGTACAAGATCAACGGCAACGGCCCCCTCTGGCCGGGATATACCGTTGAGGCGACAAAGGGGAG
CCCGGCCACGGCGACGTATGTGAACAACCTGGCAGGGCCGGGAGGGACACCGCCGCTGCTGCAGAAGTATCTGACGGTGG
ACCAGACGATCCACTGGGCCGATCCGTTCAAGCTCAGGTGCATGTTTACTCCCATGGCCGCCGGGTGTTTCGACCCCTAT
ACCGGTCCGGTGCCGGCGGTCGCTCACCTCCATGGCGGCGAGGACAAGTCGGACTTCGACGGCGGACCGGAGCAGTGGTT
CACGCCGAACGGGATGCGTGGCCCGGCCTACAGGACCTACACCTCGGCAGGCGCGCCGCCAGCCCCGGCAAACGGAGCGG
TCTACAACTACCCGAACGGCCAGGAAGCAACAACCCTCTGGTTCCATGACCACGCCCTCGGCACGACCCGGCTCAACGTC
TATGGCGGCCTGGCAGCCTTCTACTTCCTTCGGGACGACCGCGATACCGGCCGAGCGGATAACCCGATCCGCCTTCCCGC
CGGCGACCAGGAGACCGAGATCCTCATCCAGGACCGGCAGTTCGACACCGCCGGCCAACTCCTCTTCCCCGACGGCTTCC
CGTCCGGGCTGAACGGCGCGCCACCTAACCCCACGGTTCATCCGTTCTGGATTCCCGAATTTTTCGGCGACGTCATCGTG
GTGAACGGGAAATCGTGGCCCTATCAGAACGTTGAGCCGAGGCGCTATCGCCTCCGTCTCCTCAACGGCTCCAACGCCCG
TTTCTATAACCTGGCCTTCATTGATGGGGTCCTGCGCAGCGCACCCATCTGGCAGATCGGCACCGATGGCGGGTTACTCG
ACGCGCCGGTCCCTATTGTCTATCCTGGCCGCCTGCTGCTGGCACCGGGTGAGCGGGCCGATGTCATCGTCGATTTTACC
CCCTTTGCCGGGAAAACCGTTACTCTCACGAACAATGCCAAGGCCCCCTTCCCGAGCGGCATGGCCGCTGACCCGCAGAC
CACCGCCCAGATAATGCAGTTCAGGGTAGGGACGACAGTCACCGGCGACACTGACGGAACCTGTGACCCGGCAGCCTCCA
AGAAGAGCGGCAATAAATGCGTCCTCCGGACAACACCCATGGTGAAACTGGCGACCGCCAAGGGAACCACAGCCCCCGGT
GTGACCGTTGCCACGCGGCGGCAACTGGTGCTGAGGGAAATAGAAGGGGGAGGCGGGCCGCTGGAGGTGCTCCTCAACAA
CACCAAGTGGAGCGGCATCATGGAGAGCACGATGGGCACCACGAATACCCCGGTTGCCGGAAGCACGCAGCTGGTTGATT
ACTGGCTCACCGAACTTCCCACCCTGGGCTCAACCGAGGTCTGGGAGATCATCAACACGACGGGCGATGCCCACCCGATC
CACCTCCACCTCGTGCAGTTCCAGATCATGAACCGTCAGCCCTACCAGGCGAACAAGTACCTGTCGGACTGGATGGCGGC
TTTGACCGCAGCAGGGAAAGGCCCGGGAGACGGCCCTCCCAACCCGTACGCCACTGTCCTCACGGATACCCTCTCCATCG
GCGGGCAGAACCAGGTGATAGGCGGCAACCTGCTCGTCACCCCCTATCTTCAGGGCTCACCCGTGCCGGCAGCTCCGAAC
GAGCAGGGCTGGAAGGATACGGTGGTCATGATGCCGGGAGAGGTTACCCGGATCGCCGTGCGTTTCTCGCCCCAGGGCAA
CCCGATCGGCGGTTCCGTCGGCTACTCCTTCGACGCCACCGATGGCCCCGGTTACGTCTGGCATTGCCATATCCTCGACC
ACGAGGACAACGAGATGATGCGGCCGTATATCCCCGTCAAGCCCTAA

Upstream 100 bases:

>100_bases
GATCAATGCCATGACGGCACCGGCAGAAAATTGCCGGCTCCCCAGGGACGGGGATTTTATGGAAGTGCGGCACATACTGT
CAGCGAAAAAGGAGTCAACC

Downstream 100 bases:

>100_bases
AGCAAGACGAACAAGTACTGTGTCGCGATGCACGAAACCCGCCCTTCGGGGCGGGTTTTCGTTACCTCGACGGTGTTCGC
GAAAACAACGGAAATTCCAG

Product: multicopper oxidase, type 2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MDGRSLWRGAVAAGITLALGIGNASASFLVPQTALPGKNVPKYRDPLPTFAGSRVPATSGYTVSFEEFKQQVLPASGGFG
PTTVWGYKINGNGPLWPGYTVEATKGSPATATYVNNLAGPGGTPPLLQKYLTVDQTIHWADPFKLRCMFTPMAAGCFDPY
TGPVPAVAHLHGGEDKSDFDGGPEQWFTPNGMRGPAYRTYTSAGAPPAPANGAVYNYPNGQEATTLWFHDHALGTTRLNV
YGGLAAFYFLRDDRDTGRADNPIRLPAGDQETEILIQDRQFDTAGQLLFPDGFPSGLNGAPPNPTVHPFWIPEFFGDVIV
VNGKSWPYQNVEPRRYRLRLLNGSNARFYNLAFIDGVLRSAPIWQIGTDGGLLDAPVPIVYPGRLLLAPGERADVIVDFT
PFAGKTVTLTNNAKAPFPSGMAADPQTTAQIMQFRVGTTVTGDTDGTCDPAASKKSGNKCVLRTTPMVKLATAKGTTAPG
VTVATRRQLVLREIEGGGGPLEVLLNNTKWSGIMESTMGTTNTPVAGSTQLVDYWLTELPTLGSTEVWEIINTTGDAHPI
HLHLVQFQIMNRQPYQANKYLSDWMAALTAAGKGPGDGPPNPYATVLTDTLSIGGQNQVIGGNLLVTPYLQGSPVPAAPN
EQGWKDTVVMMPGEVTRIAVRFSPQGNPIGGSVGYSFDATDGPGYVWHCHILDHEDNEMMRPYIPVKP

Sequences:

>Translated_708_residues
MDGRSLWRGAVAAGITLALGIGNASASFLVPQTALPGKNVPKYRDPLPTFAGSRVPATSGYTVSFEEFKQQVLPASGGFG
PTTVWGYKINGNGPLWPGYTVEATKGSPATATYVNNLAGPGGTPPLLQKYLTVDQTIHWADPFKLRCMFTPMAAGCFDPY
TGPVPAVAHLHGGEDKSDFDGGPEQWFTPNGMRGPAYRTYTSAGAPPAPANGAVYNYPNGQEATTLWFHDHALGTTRLNV
YGGLAAFYFLRDDRDTGRADNPIRLPAGDQETEILIQDRQFDTAGQLLFPDGFPSGLNGAPPNPTVHPFWIPEFFGDVIV
VNGKSWPYQNVEPRRYRLRLLNGSNARFYNLAFIDGVLRSAPIWQIGTDGGLLDAPVPIVYPGRLLLAPGERADVIVDFT
PFAGKTVTLTNNAKAPFPSGMAADPQTTAQIMQFRVGTTVTGDTDGTCDPAASKKSGNKCVLRTTPMVKLATAKGTTAPG
VTVATRRQLVLREIEGGGGPLEVLLNNTKWSGIMESTMGTTNTPVAGSTQLVDYWLTELPTLGSTEVWEIINTTGDAHPI
HLHLVQFQIMNRQPYQANKYLSDWMAALTAAGKGPGDGPPNPYATVLTDTLSIGGQNQVIGGNLLVTPYLQGSPVPAAPN
EQGWKDTVVMMPGEVTRIAVRFSPQGNPIGGSVGYSFDATDGPGYVWHCHILDHEDNEMMRPYIPVKP
>Mature_708_residues
MDGRSLWRGAVAAGITLALGIGNASASFLVPQTALPGKNVPKYRDPLPTFAGSRVPATSGYTVSFEEFKQQVLPASGGFG
PTTVWGYKINGNGPLWPGYTVEATKGSPATATYVNNLAGPGGTPPLLQKYLTVDQTIHWADPFKLRCMFTPMAAGCFDPY
TGPVPAVAHLHGGEDKSDFDGGPEQWFTPNGMRGPAYRTYTSAGAPPAPANGAVYNYPNGQEATTLWFHDHALGTTRLNV
YGGLAAFYFLRDDRDTGRADNPIRLPAGDQETEILIQDRQFDTAGQLLFPDGFPSGLNGAPPNPTVHPFWIPEFFGDVIV
VNGKSWPYQNVEPRRYRLRLLNGSNARFYNLAFIDGVLRSAPIWQIGTDGGLLDAPVPIVYPGRLLLAPGERADVIVDFT
PFAGKTVTLTNNAKAPFPSGMAADPQTTAQIMQFRVGTTVTGDTDGTCDPAASKKSGNKCVLRTTPMVKLATAKGTTAPG
VTVATRRQLVLREIEGGGGPLEVLLNNTKWSGIMESTMGTTNTPVAGSTQLVDYWLTELPTLGSTEVWEIINTTGDAHPI
HLHLVQFQIMNRQPYQANKYLSDWMAALTAAGKGPGDGPPNPYATVLTDTLSIGGQNQVIGGNLLVTPYLQGSPVPAAPN
EQGWKDTVVMMPGEVTRIAVRFSPQGNPIGGSVGYSFDATDGPGYVWHCHILDHEDNEMMRPYIPVKP

Specific function: Involved in brown pigmentation during sporogenesis [H]

COG id: COG2132

COG function: function code Q; Putative multicopper oxidases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To S.antibioticus phenoxazinone synthase (phsA) [H]

Homologues:

Organism=Escherichia coli, GI1786314, Length=425, Percent_Identity=27.5294117647059, Blast_Score=88, Evalue=2e-18,
Organism=Escherichia coli, GI1789394, Length=198, Percent_Identity=34.3434343434343, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001117
- InterPro:   IPR011706
- InterPro:   IPR011707
- InterPro:   IPR008972 [H]

Pfam domain/function: PF00394 Cu-oxidase; PF07731 Cu-oxidase_2; PF07732 Cu-oxidase_3 [H]

EC number: NA

Molecular weight: Translated: 75887; Mature: 75887

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGRSLWRGAVAAGITLALGIGNASASFLVPQTALPGKNVPKYRDPLPTFAGSRVPATSG
CCCHHHHHHHHHHCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YTVSFEEFKQQVLPASGGFGPTTVWGYKINGNGPLWPGYTVEATKGSPATATYVNNLAGP
CEEEHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHCCCC
GGTPPLLQKYLTVDQTIHWADPFKLRCMFTPMAAGCFDPYTGPVPAVAHLHGGEDKSDFD
CCCHHHHHHHHHHCCEEEECCCEEEEEEECCHHCCCCCCCCCCCCEEEEECCCCCCCCCC
GGPEQWFTPNGMRGPAYRTYTSAGAPPAPANGAVYNYPNGQEATTLWFHDHALGTTRLNV
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCEEEEEE
YGGLAAFYFLRDDRDTGRADNPIRLPAGDQETEILIQDRQFDTAGQLLFPDGFPSGLNGA
HCCEEEEEEEECCCCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCC
PPNPTVHPFWIPEFFGDVIVVNGKSWPYQNVEPRRYRLRLLNGSNARFYNLAFIDGVLRS
CCCCCCCEEECHHHHCCEEEECCCCCCCCCCCCCEEEEEEEECCCCEEEEEHHHHHHHHC
APIWQIGTDGGLLDAPVPIVYPGRLLLAPGERADVIVDFTPFAGKTVTLTNNAKAPFPSG
CCEEEECCCCCEECCCCCEEECCEEEECCCCCCCEEEEECCCCCCEEEEECCCCCCCCCC
MAADPQTTAQIMQFRVGTTVTGDTDGTCDPAASKKSGNKCVLRTTPMVKLATAKGTTAPG
CCCCCHHHHHHHHEECCCEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCC
VTVATRRQLVLREIEGGGGPLEVLLNNTKWSGIMESTMGTTNTPVAGSTQLVDYWLTELP
EEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCC
TLGSTEVWEIINTTGDAHPIHLHLVQFQIMNRQPYQANKYLSDWMAALTAAGKGPGDGPP
CCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
NPYATVLTDTLSIGGQNQVIGGNLLVTPYLQGSPVPAAPNEQGWKDTVVMMPGEVTRIAV
CCCEEEEEEHHHCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEE
RFSPQGNPIGGSVGYSFDATDGPGYVWHCHILDHEDNEMMRPYIPVKP
EECCCCCCCCCCCCCEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MDGRSLWRGAVAAGITLALGIGNASASFLVPQTALPGKNVPKYRDPLPTFAGSRVPATSG
CCCHHHHHHHHHHCEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YTVSFEEFKQQVLPASGGFGPTTVWGYKINGNGPLWPGYTVEATKGSPATATYVNNLAGP
CEEEHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCEEEEECCCCCCCHHHHHHCCCC
GGTPPLLQKYLTVDQTIHWADPFKLRCMFTPMAAGCFDPYTGPVPAVAHLHGGEDKSDFD
CCCHHHHHHHHHHCCEEEECCCEEEEEEECCHHCCCCCCCCCCCCEEEEECCCCCCCCCC
GGPEQWFTPNGMRGPAYRTYTSAGAPPAPANGAVYNYPNGQEATTLWFHDHALGTTRLNV
CCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCEEEEEE
YGGLAAFYFLRDDRDTGRADNPIRLPAGDQETEILIQDRQFDTAGQLLFPDGFPSGLNGA
HCCEEEEEEEECCCCCCCCCCCEECCCCCCCCEEEEECCCCCCCCCEEECCCCCCCCCCC
PPNPTVHPFWIPEFFGDVIVVNGKSWPYQNVEPRRYRLRLLNGSNARFYNLAFIDGVLRS
CCCCCCCEEECHHHHCCEEEECCCCCCCCCCCCCEEEEEEEECCCCEEEEEHHHHHHHHC
APIWQIGTDGGLLDAPVPIVYPGRLLLAPGERADVIVDFTPFAGKTVTLTNNAKAPFPSG
CCEEEECCCCCEECCCCCEEECCEEEECCCCCCCEEEEECCCCCCEEEEECCCCCCCCCC
MAADPQTTAQIMQFRVGTTVTGDTDGTCDPAASKKSGNKCVLRTTPMVKLATAKGTTAPG
CCCCCHHHHHHHHEECCCEEECCCCCCCCCCCCCCCCCEEEEEECCCEEEEECCCCCCCC
VTVATRRQLVLREIEGGGGPLEVLLNNTKWSGIMESTMGTTNTPVAGSTQLVDYWLTELP
EEHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHCC
TLGSTEVWEIINTTGDAHPIHLHLVQFQIMNRQPYQANKYLSDWMAALTAAGKGPGDGPP
CCCCHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
NPYATVLTDTLSIGGQNQVIGGNLLVTPYLQGSPVPAAPNEQGWKDTVVMMPGEVTRIAV
CCCEEEEEEHHHCCCCCEEECCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEE
RFSPQGNPIGGSVGYSFDATDGPGYVWHCHILDHEDNEMMRPYIPVKP
EECCCCCCCCCCCCCEEECCCCCCEEEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969499; 9455482; 9384377; 2821284; 3135411 [H]