Click here to switch to the map view.

The map label for this gene is yvkC [H]

Identifier: 78223307

GI number: 78223307

Start: 2364923

End: 2366752

Strand: Direct

Name: yvkC [H]

Synonym: Gmet_2100

Alternate gene names: 78223307

Gene position: 2364923-2366752 (Clockwise)

Preceding gene: 78223304

Following gene: 78223308

Centisome position: 59.16

GC content: 59.62

Gene sequence:

>1830_bases
ATGAAATTTCCAAAACCACATGATGTCAGGACAATCCCTGGAACAGAAGGTTGGGAGAGAATGTATCCGTACCAGTACCA
GTTCGTCACTGACGACCCGGAACGGATGACGTACGAGGCGCAATCCTTCTGGTTTTATGATGGACTCCACTATCCCGAAC
CGATCTACCCCTTCGACACGATCTGGGATGAGGCCTGGTACCTGGGGTTGTCCCAGTACAACAACCGCATCTTCCAGGTG
CCGCCGGTGCGCGGCGTCGACCACCGGATCATCAACGGCTACGTGTATATCTCGCCGGTTCCGGTAACAGACCCGGAAGA
GGTAGGGAGCCGCGTACCCAACTTCATGGAGCGCGCGGGCTATTACTACAAAAACTGGAATGAGCTGGAGGCCAAGTGGG
AACTGAAGATGAAAGGGATCATCAAGCAGATCGAAGAACTGCCGATCCCCAGCCTCCCCGATCTTGAAGATATCAGCGTG
GTCACTGAAGGAATCGGCGAATCAAAGGGATTCCATCTGATCAAAGCCTACGACGACCTGATCAACCTGGGTATCCAGTG
CTGGCAGTACCACTTCGAATTCCTCAACCTCGGCTATGCCGCCTACGTCTTTTTTCTCGACTTCGTGCAAAAGCTCTTCC
CGAGCATCCCGCTGCAGCGGGTGACCCAGATGATCGCCGGTATCGACGTGATCATGTACCGCTCCGACGACGAGCTGAAA
AAACTGGCCAAGAAAGCCATCGAACTGGGGGTCGACCTGATCCTGACCAACTGCGGGGAGTGGCAGGATGCCGAGACCGC
GCTGAAGGCGCATCCGAAGGGGAGGACCTGGCTGGATGCCCTGGAAGAGGCCCGGTATCCCTGGTTTCACGTCTCCACCG
GCACCGGCTGGTCCCACAGCGACAAGTCGTGGAACGACGACCTGAACATTCCGCTTTCCGGCATCGCCACCTATATCAAT
CAGCTCAGGCAGGGGATGGACATAGAACGGCCGATGGAAAAGGTGCGAGCCGAACGGGACCGGATCACCGAGGAGTACCG
CGACCTCATTACATCGGACGAGGACCGCAAGACCTTCGACGAACTGCTCGGCACCGCCAAGACGGTCTTCCCCTATGTGG
AGAACCACCTCTTCTACGTCGAGCACTGGTTCCACACCGTGTTCTGGAGAAAAATGCGCGAAGTGGCGGCCATCATGAAA
GAGCACGGCATGTTCGAGGACGTGGAGGATGTCTGGTATCTGCGCCGGGATGAAATCAAGCAGGGGCTGTGGGACATGGT
CACCGCCTGGGCAACCGGCGTCAAGCCCCGGGGCACCGCGGTATGGCCCAAGGAGATCGCCTGGCGCAAAGGGGTGATGA
AAAAGTTCCAGGAGTGGAGTCCGCCGCCGGCCGTCGGTATCCCCCCCGAAGTGATCCGGGAGCCGTTCACCATCGTGCTC
TGGGGGGTCACCAACTCCTCCATCTCGGACTGGTCCGAAGTACAGGACATCTCCGATCCGGACAGCATCACCACCCTGAA
AGGGTTTGCCGGCAGCCCCGGCGTGGTGGAGGGGAAAGCCCGGGTCTGCCGCAGCGCGGACGACATCCGCCTGCTGCAGG
AGGGAGAAATTCTAGTGGCCCCCACCACCTCGCCCTCCTGGGCACCGGCCTTTGCCAGGATCAAGGGGGCAATCACCGAT
GTGGGGGGAGTCATGAGCCACGCGGCAATCGTCTGCAGGGAATACGGCATGCCGGCGGTGGTCGGCACCGGCCACGCGAC
GAAGATCATCCGGACCGGCATGATGCTCCGGGTCGACGGCGCCACCGGCGTCGTCACCATAGACCGCTGA

Upstream 100 bases:

>100_bases
CCCAAATGTCACACTCACCACAAACCAGAACCTTCGTCGACGGCAACGAGGCCTCGCATCACTAACCATGGAGCATCAAC
GTACATTCGGGAGACACGCG

Downstream 100 bases:

>100_bases
GAAAACCGGACAGAGGAGGCAATGGACATGAGCACTGCAGTGAACATGCATGACAAAAGGTGGGGAAACAAGTACGGAAC
CGTCCGTTCGGCAGGGAAGG

Product: hypothetical protein

Products: AMP; H+; Phosphoenolpyruvate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 609; Mature: 609

Protein sequence:

>609_residues
MKFPKPHDVRTIPGTEGWERMYPYQYQFVTDDPERMTYEAQSFWFYDGLHYPEPIYPFDTIWDEAWYLGLSQYNNRIFQV
PPVRGVDHRIINGYVYISPVPVTDPEEVGSRVPNFMERAGYYYKNWNELEAKWELKMKGIIKQIEELPIPSLPDLEDISV
VTEGIGESKGFHLIKAYDDLINLGIQCWQYHFEFLNLGYAAYVFFLDFVQKLFPSIPLQRVTQMIAGIDVIMYRSDDELK
KLAKKAIELGVDLILTNCGEWQDAETALKAHPKGRTWLDALEEARYPWFHVSTGTGWSHSDKSWNDDLNIPLSGIATYIN
QLRQGMDIERPMEKVRAERDRITEEYRDLITSDEDRKTFDELLGTAKTVFPYVENHLFYVEHWFHTVFWRKMREVAAIMK
EHGMFEDVEDVWYLRRDEIKQGLWDMVTAWATGVKPRGTAVWPKEIAWRKGVMKKFQEWSPPPAVGIPPEVIREPFTIVL
WGVTNSSISDWSEVQDISDPDSITTLKGFAGSPGVVEGKARVCRSADDIRLLQEGEILVAPTTSPSWAPAFARIKGAITD
VGGVMSHAAIVCREYGMPAVVGTGHATKIIRTGMMLRVDGATGVVTIDR

Sequences:

>Translated_609_residues
MKFPKPHDVRTIPGTEGWERMYPYQYQFVTDDPERMTYEAQSFWFYDGLHYPEPIYPFDTIWDEAWYLGLSQYNNRIFQV
PPVRGVDHRIINGYVYISPVPVTDPEEVGSRVPNFMERAGYYYKNWNELEAKWELKMKGIIKQIEELPIPSLPDLEDISV
VTEGIGESKGFHLIKAYDDLINLGIQCWQYHFEFLNLGYAAYVFFLDFVQKLFPSIPLQRVTQMIAGIDVIMYRSDDELK
KLAKKAIELGVDLILTNCGEWQDAETALKAHPKGRTWLDALEEARYPWFHVSTGTGWSHSDKSWNDDLNIPLSGIATYIN
QLRQGMDIERPMEKVRAERDRITEEYRDLITSDEDRKTFDELLGTAKTVFPYVENHLFYVEHWFHTVFWRKMREVAAIMK
EHGMFEDVEDVWYLRRDEIKQGLWDMVTAWATGVKPRGTAVWPKEIAWRKGVMKKFQEWSPPPAVGIPPEVIREPFTIVL
WGVTNSSISDWSEVQDISDPDSITTLKGFAGSPGVVEGKARVCRSADDIRLLQEGEILVAPTTSPSWAPAFARIKGAITD
VGGVMSHAAIVCREYGMPAVVGTGHATKIIRTGMMLRVDGATGVVTIDR
>Mature_609_residues
MKFPKPHDVRTIPGTEGWERMYPYQYQFVTDDPERMTYEAQSFWFYDGLHYPEPIYPFDTIWDEAWYLGLSQYNNRIFQV
PPVRGVDHRIINGYVYISPVPVTDPEEVGSRVPNFMERAGYYYKNWNELEAKWELKMKGIIKQIEELPIPSLPDLEDISV
VTEGIGESKGFHLIKAYDDLINLGIQCWQYHFEFLNLGYAAYVFFLDFVQKLFPSIPLQRVTQMIAGIDVIMYRSDDELK
KLAKKAIELGVDLILTNCGEWQDAETALKAHPKGRTWLDALEEARYPWFHVSTGTGWSHSDKSWNDDLNIPLSGIATYIN
QLRQGMDIERPMEKVRAERDRITEEYRDLITSDEDRKTFDELLGTAKTVFPYVENHLFYVEHWFHTVFWRKMREVAAIMK
EHGMFEDVEDVWYLRRDEIKQGLWDMVTAWATGVKPRGTAVWPKEIAWRKGVMKKFQEWSPPPAVGIPPEVIREPFTIVL
WGVTNSSISDWSEVQDISDPDSITTLKGFAGSPGVVEGKARVCRSADDIRLLQEGEILVAPTTSPSWAPAFARIKGAITD
VGGVMSHAAIVCREYGMPAVVGTGHATKIIRTGMMLRVDGATGVVTIDR

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=221, Percent_Identity=27.1493212669683, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17564524, Length=380, Percent_Identity=24.4736842105263, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2 [C]

Molecular weight: Translated: 69976; Mature: 69976

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFPKPHDVRTIPGTEGWERMYPYQYQFVTDDPERMTYEAQSFWFYDGLHYPEPIYPFDT
CCCCCCCCCEECCCCCCHHHCCCEEEEEECCCHHHHEEHHHHEEEECCCCCCCCCCCHHH
IWDEAWYLGLSQYNNRIFQVPPVRGVDHRIINGYVYISPVPVTDPEEVGSRVPNFMERAG
HHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHCHHHHHHCC
YYYKNWNELEAKWELKMKGIIKQIEELPIPSLPDLEDISVVTEGIGESKGFHLIKAYDDL
CEECCCCHHCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEHHHHHHHH
INLGIQCWQYHFEFLNLGYAAYVFFLDFVQKLFPSIPLQRVTQMIAGIDVIMYRSDDELK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCHHHH
KLAKKAIELGVDLILTNCGEWQDAETALKAHPKGRTWLDALEEARYPWFHVSTGTGWSHS
HHHHHHHHHCHHHEEECCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCC
DKSWNDDLNIPLSGIATYINQLRQGMDIERPMEKVRAERDRITEEYRDLITSDEDRKTFD
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELLGTAKTVFPYVENHLFYVEHWFHTVFWRKMREVAAIMKEHGMFEDVEDVWYLRRDEIK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QGLWDMVTAWATGVKPRGTAVWPKEIAWRKGVMKKFQEWSPPPAVGIPPEVIREPFTIVL
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEE
WGVTNSSISDWSEVQDISDPDSITTLKGFAGSPGVVEGKARVCRSADDIRLLQEGEILVA
EECCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHCCCHHHEEEECCCEEEE
PTTSPSWAPAFARIKGAITDVGGVMSHAAIVCREYGMPAVVGTGHATKIIRTGMMLRVDG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCEEEECC
ATGVVTIDR
CCEEEEECC
>Mature Secondary Structure
MKFPKPHDVRTIPGTEGWERMYPYQYQFVTDDPERMTYEAQSFWFYDGLHYPEPIYPFDT
CCCCCCCCCEECCCCCCHHHCCCEEEEEECCCHHHHEEHHHHEEEECCCCCCCCCCCHHH
IWDEAWYLGLSQYNNRIFQVPPVRGVDHRIINGYVYISPVPVTDPEEVGSRVPNFMERAG
HHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHCHHHHHHCC
YYYKNWNELEAKWELKMKGIIKQIEELPIPSLPDLEDISVVTEGIGESKGFHLIKAYDDL
CEECCCCHHCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCEEHHHHHHHH
INLGIQCWQYHFEFLNLGYAAYVFFLDFVQKLFPSIPLQRVTQMIAGIDVIMYRSDDELK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEECCCHHHH
KLAKKAIELGVDLILTNCGEWQDAETALKAHPKGRTWLDALEEARYPWFHVSTGTGWSHS
HHHHHHHHHCHHHEEECCCCCCCHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCCCC
DKSWNDDLNIPLSGIATYINQLRQGMDIERPMEKVRAERDRITEEYRDLITSDEDRKTFD
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
ELLGTAKTVFPYVENHLFYVEHWFHTVFWRKMREVAAIMKEHGMFEDVEDVWYLRRDEIK
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
QGLWDMVTAWATGVKPRGTAVWPKEIAWRKGVMKKFQEWSPPPAVGIPPEVIREPFTIVL
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCEEEEE
WGVTNSSISDWSEVQDISDPDSITTLKGFAGSPGVVEGKARVCRSADDIRLLQEGEILVA
EECCCCCCCCHHHHHCCCCCCCEEEECCCCCCCCCCCCHHHHHCCCHHHEEEECCCEEEE
PTTSPSWAPAFARIKGAITDVGGVMSHAAIVCREYGMPAVVGTGHATKIIRTGMMLRVDG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHCCCEEEECC
ATGVVTIDR
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.028 {ATP}} 0.083 {pyruvate}} [C]

Substrates: ATP; H2O; Pyruvate [C]

Specific reaction: ATP + H2O + Pyruvate --> AMP + (2) H+ + Phosphoenolpyruvate + Phosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]