BacMap

Definition	Geobacter metallireducens GS-15 chromosome, complete genome.
Accession	NC_007517
Length	3,997,420

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The map label for this gene is crt [H]

Identifier: 78223264

GI number: 78223264

Start: 2311305

End: 2312141

Strand: Reverse

Name: crt [H]

Synonym: Gmet_2057

Alternate gene names: 78223264

Gene position: 2312141-2311305 (Counterclockwise)

Preceding gene: 78223265

Following gene: 78223263

Centisome position: 57.84

GC content: 62.37

Gene sequence:

>837_bases
ATGAACGGTGAAGCATTAACTATTGCGGCTGTGCCGCTGTTGCAGGATTCATCCCTGGAGCTGCACGGGGGGGTAGCAGT
CCTGACCTTCCAGCGGGACGATGTGCGCAACGCCCTGACCGGGACAGCCCTTGTCGATGATCTCGTGCGGACGGTGCAGT
GGGCCAACACCTCGACGGAAGTTTCGGTGCTGGTCATCACGGGCGCAGGGGCAGCATTTTCTTCCGGCGGCAATGTCAAG
GAGATGCAGGAAAAGAGCGGCATCTTTGCCGGCGGTCCCATTGATGTCCAGAACCAGTACCGGCGCGGCATTCAGCAGGT
GCCGCTGGCCCTCCACAAGGCAGAGATCCCGGTCATTGCCGCCGTCAATGGGCCGGCCATGGGGGCCGGTCTCGACCTGG
CCTGCATGTGCGATATCCGCATCGCCTCGGACAAGGCTTTGATTGGTGGAACCTTCATCAATCTCGGCATCATCCCGGGG
GATGGGGGGGCCTGGTTCCTTCCCCGACTTGTAGGGTACCAGCGGGCCGCGGAGATGTTGTTCACCGGAAGGATCATGAA
GGCTGCGGAGGCGCTGCAACTGGGCCTGTTCCTGGAGGTCGTGGAACCGGAGGAGTTGCTCCCGCGGGCACGGGAGCTGG
CCGGTCAGATTGCCGCCAAACCTCCCCAGGCATTGCGGCTGATGAAACGTCTGCTGAAACTGGCCCAACGCAACGACCTG
CCGGATTTCCTCGATGCGACCGCCTGTTTCCAGGCCATGGCCCACCATTCAGACGATCATCTGGAGGCGGTCACGGCCTT
TCTGGAAAAACGGGCGCCCCGCTACAGCGGACGGTAG

Upstream 100 bases:

>100_bases
GACAGGGAATTGCGACGATCTTCGAACGCGTGTAAGTGAACAATGGGAGGTGGCATGGAGATCATGTCACCTCTTTTTTT
ATGGGAGTTTGATATCCAGC

Downstream 100 bases:

>100_bases
TGGCCGCCAGTGTTGAATATTCAGCGGCCGGGAGCGCGATTATGACCTCTGAGATTATCGATGTTCACATGCATTGTTTT
GTGGGCCGCCAGCAGGAGAC

Product: short chain enoyl-CoA hydratase/enoyl-CoA hydratase

Products: NA

Alternate protein names: Crotonase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MNGEALTIAAVPLLQDSSLELHGGVAVLTFQRDDVRNALTGTALVDDLVRTVQWANTSTEVSVLVITGAGAAFSSGGNVK
EMQEKSGIFAGGPIDVQNQYRRGIQQVPLALHKAEIPVIAAVNGPAMGAGLDLACMCDIRIASDKALIGGTFINLGIIPG
DGGAWFLPRLVGYQRAAEMLFTGRIMKAAEALQLGLFLEVVEPEELLPRARELAGQIAAKPPQALRLMKRLLKLAQRNDL
PDFLDATACFQAMAHHSDDHLEAVTAFLEKRAPRYSGR

Sequences:

>Translated_278_residues
MNGEALTIAAVPLLQDSSLELHGGVAVLTFQRDDVRNALTGTALVDDLVRTVQWANTSTEVSVLVITGAGAAFSSGGNVK
EMQEKSGIFAGGPIDVQNQYRRGIQQVPLALHKAEIPVIAAVNGPAMGAGLDLACMCDIRIASDKALIGGTFINLGIIPG
DGGAWFLPRLVGYQRAAEMLFTGRIMKAAEALQLGLFLEVVEPEELLPRARELAGQIAAKPPQALRLMKRLLKLAQRNDL
PDFLDATACFQAMAHHSDDHLEAVTAFLEKRAPRYSGR
>Mature_278_residues
MNGEALTIAAVPLLQDSSLELHGGVAVLTFQRDDVRNALTGTALVDDLVRTVQWANTSTEVSVLVITGAGAAFSSGGNVK
EMQEKSGIFAGGPIDVQNQYRRGIQQVPLALHKAEIPVIAAVNGPAMGAGLDLACMCDIRIASDKALIGGTFINLGIIPG
DGGAWFLPRLVGYQRAAEMLFTGRIMKAAEALQLGLFLEVVEPEELLPRARELAGQIAAKPPQALRLMKRLLKLAQRNDL
PDFLDATACFQAMAHHSDDHLEAVTAFLEKRAPRYSGR

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=255, Percent_Identity=31.7647058823529, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI31542718, Length=258, Percent_Identity=32.1705426356589, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI4502327, Length=263, Percent_Identity=31.1787072243346, Blast_Score=94, Evalue=9e-20,
Organism=Homo sapiens, GI68989263, Length=247, Percent_Identity=30.7692307692308, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI45643119, Length=250, Percent_Identity=26.8, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI260275230, Length=250, Percent_Identity=26.8, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI260274832, Length=250, Percent_Identity=26.8, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI20127408, Length=152, Percent_Identity=32.2368421052632, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI70995211, Length=250, Percent_Identity=27.6, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI150378541, Length=264, Percent_Identity=28.030303030303, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI116235487, Length=200, Percent_Identity=27.5, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI157694516, Length=217, Percent_Identity=26.7281105990783, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI213417737, Length=217, Percent_Identity=26.7281105990783, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI62530384, Length=249, Percent_Identity=26.5060240963855, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI37594469, Length=193, Percent_Identity=27.979274611399, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI37594471, Length=193, Percent_Identity=27.979274611399, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI221307494, Length=103, Percent_Identity=35.9223300970874, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI221136756, Length=129, Percent_Identity=32.5581395348837, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI221136753, Length=129, Percent_Identity=32.5581395348837, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI261878539, Length=140, Percent_Identity=35, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI295842266, Length=223, Percent_Identity=26.0089686098655, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=38.4313725490196, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=33.852140077821, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1788597, Length=267, Percent_Identity=29.9625468164794, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI221142681, Length=225, Percent_Identity=34.2222222222222, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788682, Length=148, Percent_Identity=39.1891891891892, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI87082183, Length=259, Percent_Identity=27.4131274131274, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790281, Length=196, Percent_Identity=30.6122448979592, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=255, Percent_Identity=33.3333333333333, Blast_Score=115, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17554946, Length=256, Percent_Identity=31.640625, Blast_Score=111, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=234, Percent_Identity=28.6324786324786, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17535521, Length=257, Percent_Identity=27.2373540856031, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17558304, Length=189, Percent_Identity=31.2169312169312, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=229, Percent_Identity=27.9475982532751, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=221, Percent_Identity=27.6018099547511, Blast_Score=81, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17560910, Length=205, Percent_Identity=30.7317073170732, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=198, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17508951, Length=198, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17508953, Length=198, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=201, Percent_Identity=29.8507462686567, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17549921, Length=220, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=200, Percent_Identity=26, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=200, Percent_Identity=26, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129971, Length=260, Percent_Identity=35.3846153846154, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653477, Length=260, Percent_Identity=35.3846153846154, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24653139, Length=260, Percent_Identity=30.3846153846154, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24654903, Length=219, Percent_Identity=26.4840182648402, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357171, Length=212, Percent_Identity=30.188679245283, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24650670, Length=207, Percent_Identity=27.0531400966184, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920382, Length=226, Percent_Identity=29.2035398230088, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI28571729, Length=146, Percent_Identity=29.4520547945205, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28571730, Length=146, Percent_Identity=29.4520547945205, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI19922422, Length=266, Percent_Identity=25.9398496240602, Blast_Score=66, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24583165, Length=233, Percent_Identity=27.4678111587983, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.55 [H]

Molecular weight: Translated: 29719; Mature: 29719

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGEALTIAAVPLLQDSSLELHGGVAVLTFQRDDVRNALTGTALVDDLVRTVQWANTSTE
CCCCEEEEEEEEEECCCCEEEECCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VSVLVITGAGAAFSSGGNVKEMQEKSGIFAGGPIDVQNQYRRGIQQVPLALHKAEIPVIA
EEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCEEE
AVNGPAMGAGLDLACMCDIRIASDKALIGGTFINLGIIPGDGGAWFLPRLVGYQRAAEML
EECCCCCCCCCCEEEEEEEEECCCCEEECEEEEEEEEEECCCCCCHHHHHHHHHHHHHHH
FTGRIMKAAEALQLGLFLEVVEPEELLPRARELAGQIAAKPPQALRLMKRLLKLAQRNDL
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PDFLDATACFQAMAHHSDDHLEAVTAFLEKRAPRYSGR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNGEALTIAAVPLLQDSSLELHGGVAVLTFQRDDVRNALTGTALVDDLVRTVQWANTSTE
CCCCEEEEEEEEEECCCCEEEECCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCE
VSVLVITGAGAAFSSGGNVKEMQEKSGIFAGGPIDVQNQYRRGIQQVPLALHKAEIPVIA
EEEEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHCCCCEEE
AVNGPAMGAGLDLACMCDIRIASDKALIGGTFINLGIIPGDGGAWFLPRLVGYQRAAEML
EECCCCCCCCCCEEEEEEEEECCCCEEECEEEEEEEEEECCCCCCHHHHHHHHHHHHHHH
FTGRIMKAAEALQLGLFLEVVEPEELLPRARELAGQIAAKPPQALRLMKRLLKLAQRNDL
HHHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCC
PDFLDATACFQAMAHHSDDHLEAVTAFLEKRAPRYSGR
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8655474; 11466286 [H]