Click here to switch to the map view.

The map label for this gene is capL [H]

Identifier: 78223201

GI number: 78223201

Start: 2226688

End: 2227977

Strand: Reverse

Name: capL [H]

Synonym: Gmet_1994

Alternate gene names: 78223201

Gene position: 2227977-2226688 (Counterclockwise)

Preceding gene: 78223203

Following gene: 78223200

Centisome position: 55.74

GC content: 61.01

Gene sequence:

>1290_bases
ATGAATTCACTCAATCGTACGGTTTCCGTCGTGGGCCTCGGCTACGTGGGGCTACCCGTGGCGGTGGCTTTTGGCAAGGT
CAGGAAAACGGTCGGTTTCGACATCAACCCGGTGCGCATCAGAGAGCTCAAGGAAGGACATGACCGGACAGGGGAAGTGG
AAACGGAGGATCTGAAGGCTGCTGATATCCTTTTTACCGATTCCATCGACGAGCTGAAGCAGGCGGATTTTCATATCGTC
GCCGTGCCGACGCCGGTTGACGAGGCCAACCAGCCCGATCTCACCCTGATGCTGAAGGCATCGGAAACAGTGGGCAAGGC
CCTGGAAAAGGGTGACATCGTCGTGTACGAGTCAACGGTCTACCCCGGTGTCACCGAGGATGAGTGCGTGCCGGTGCTGG
AGAGGGTGTCGGGTCTGAAGAGCGGCAGTGACTTCACGGTTGGCTACAGCCCCGAGCGGATCAACCCCGGCGACAAGGAA
CATACCTTTACGAAGATCAAGAAAGTGGTGGCGGGGCAGGATGCGGCGACCCTGGAGACCGTCGCCCAGGTCTATGAGTC
GGTGGTGACGGCCGGGGTTCACCGGGCGGCGAGCATCAAGGTGGCCGAGGCGGCAAAGGTCATCGAGAATACCCAGCGGG
ATCTGAATATCGCCCTCATGAACGAGTTGGCAATTATCTTTGGCCGTCTCGGCATCGACACCAATGATGTGCTGGAGGCG
GCCGGAACCAAGTGGAACTTCCTGAAGTTCCGGCCGGGGCTCGTGGGCGGACACTGCATCGGCGTCGACCCCTACTATCT
GACCCACAAGGCGGAGAAGATCGGTTACATCCCCCAGGTCATTCTGGCGGGCCGCCGGATCAACGACGGCATGGGGAAGT
ATATCGCCCAGCAGGCGGTGAAGGGGATGATCCATGCGGGGCATTGCGTACGTGGCTGCACGGTGACGGTGCTGGGACTC
ACCTTCAAGGAGGACTGCCCCGATCTCCGTAACTCCAAGGTGATCGACATCATTCGCGAGTTGGAGGATTACGGCGTCGA
GGTCCAGGTGCACGACCCCTTGGCCGATGCGGGAGAGGCTTCCCACGAGTATGGCGTCCGGCTCGTCCCCTTCGGGGAGT
TGAGACCTGCGGCTGCGGTGGTTGCCGCGGTTGCGCATCGCGACTACCGGGAACTGTCTGTGGAAGGGGTCAAGGGGCTG
ATGGGGGACGGGCCGGTGCTCGTGGATGTGAAGGGTATCTATGATCCAGCCGCGGTCCGTGCCGCTGGCATCAGGCTCTG
GAGGCTGTAG

Upstream 100 bases:

>100_bases
CGAAATTCTTTACGGATATAGTGCAGTCTGGTAAAAATATATTCATTGTAGCGTGACAGCCGGCAACGCTGTCTCTGACA
ATAACTGACAAGGAGCATTC

Downstream 100 bases:

>100_bases
CGTTACGGGTAACGAATGATTCAGATCGTCCTTTCATCGATAGCTCTGCTCCTGGCTGCGGTCTACGTTTCGTGTACGCT
CTGGCGGAGCGGGCGCACCA

Product: UDP-glucose/GDP-mannose dehydrogenase

Products: UDP-N-acetyl-D-mannosaminuronic-acid; NADH; Proton [C]

Alternate protein names: NA

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MNSLNRTVSVVGLGYVGLPVAVAFGKVRKTVGFDINPVRIRELKEGHDRTGEVETEDLKAADILFTDSIDELKQADFHIV
AVPTPVDEANQPDLTLMLKASETVGKALEKGDIVVYESTVYPGVTEDECVPVLERVSGLKSGSDFTVGYSPERINPGDKE
HTFTKIKKVVAGQDAATLETVAQVYESVVTAGVHRAASIKVAEAAKVIENTQRDLNIALMNELAIIFGRLGIDTNDVLEA
AGTKWNFLKFRPGLVGGHCIGVDPYYLTHKAEKIGYIPQVILAGRRINDGMGKYIAQQAVKGMIHAGHCVRGCTVTVLGL
TFKEDCPDLRNSKVIDIIRELEDYGVEVQVHDPLADAGEASHEYGVRLVPFGELRPAAAVVAAVAHRDYRELSVEGVKGL
MGDGPVLVDVKGIYDPAAVRAAGIRLWRL

Sequences:

>Translated_429_residues
MNSLNRTVSVVGLGYVGLPVAVAFGKVRKTVGFDINPVRIRELKEGHDRTGEVETEDLKAADILFTDSIDELKQADFHIV
AVPTPVDEANQPDLTLMLKASETVGKALEKGDIVVYESTVYPGVTEDECVPVLERVSGLKSGSDFTVGYSPERINPGDKE
HTFTKIKKVVAGQDAATLETVAQVYESVVTAGVHRAASIKVAEAAKVIENTQRDLNIALMNELAIIFGRLGIDTNDVLEA
AGTKWNFLKFRPGLVGGHCIGVDPYYLTHKAEKIGYIPQVILAGRRINDGMGKYIAQQAVKGMIHAGHCVRGCTVTVLGL
TFKEDCPDLRNSKVIDIIRELEDYGVEVQVHDPLADAGEASHEYGVRLVPFGELRPAAAVVAAVAHRDYRELSVEGVKGL
MGDGPVLVDVKGIYDPAAVRAAGIRLWRL
>Mature_429_residues
MNSLNRTVSVVGLGYVGLPVAVAFGKVRKTVGFDINPVRIRELKEGHDRTGEVETEDLKAADILFTDSIDELKQADFHIV
AVPTPVDEANQPDLTLMLKASETVGKALEKGDIVVYESTVYPGVTEDECVPVLERVSGLKSGSDFTVGYSPERINPGDKE
HTFTKIKKVVAGQDAATLETVAQVYESVVTAGVHRAASIKVAEAAKVIENTQRDLNIALMNELAIIFGRLGIDTNDVLEA
AGTKWNFLKFRPGLVGGHCIGVDPYYLTHKAEKIGYIPQVILAGRRINDGMGKYIAQQAVKGMIHAGHCVRGCTVTVLGL
TFKEDCPDLRNSKVIDIIRELEDYGVEVQVHDPLADAGEASHEYGVRLVPFGELRPAAAVVAAVAHRDYRELSVEGVKGL
MGDGPVLVDVKGIYDPAAVRAAGIRLWRL

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0677

COG function: function code M; UDP-N-acetyl-D-mannosaminuronate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI48994968, Length=349, Percent_Identity=30.3724928366762, Blast_Score=144, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008927
- InterPro:   IPR016040
- InterPro:   IPR017476
- InterPro:   IPR014027
- InterPro:   IPR014026
- InterPro:   IPR014028
- InterPro:   IPR001732 [H]

Pfam domain/function: PF00984 UDPG_MGDP_dh; PF03720 UDPG_MGDP_dh_C; PF03721 UDPG_MGDP_dh_N [H]

EC number: 1.1.1.- [C]

Molecular weight: Translated: 46404; Mature: 46404

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSLNRTVSVVGLGYVGLPVAVAFGKVRKTVGFDINPVRIRELKEGHDRTGEVETEDLKA
CCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCE
ADILFTDSIDELKQADFHIVAVPTPVDEANQPDLTLMLKASETVGKALEKGDIVVYESTV
EEEEEECCHHHHHHCCEEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEECCC
YPGVTEDECVPVLERVSGLKSGSDFTVGYSPERINPGDKEHTFTKIKKVVAGQDAATLET
CCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
VAQVYESVVTAGVHRAASIKVAEAAKVIENTQRDLNIALMNELAIIFGRLGIDTNDVLEA
HHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHH
AGTKWNFLKFRPGLVGGHCIGVDPYYLTHKAEKIGYIPQVILAGRRINDGMGKYIAQQAV
CCCCEEEEEECCCCCCCEEECCCCCEEECCHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
KGMIHAGHCVRGCTVTVLGLTFKEDCPDLRNSKVIDIIRELEDYGVEVQVHDPLADAGEA
HHHHHCCCHHCCCEEEEEEEEEHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCC
SHEYGVRLVPFGELRPAAAVVAAVAHRDYRELSVEGVKGLMGDGPVLVDVKGIYDPAAVR
CHHCCEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHH
AAGIRLWRL
HHCEEEECC
>Mature Secondary Structure
MNSLNRTVSVVGLGYVGLPVAVAFGKVRKTVGFDINPVRIRELKEGHDRTGEVETEDLKA
CCCCCCEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCCE
ADILFTDSIDELKQADFHIVAVPTPVDEANQPDLTLMLKASETVGKALEKGDIVVYESTV
EEEEEECCHHHHHHCCEEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHCCCEEEEECCC
YPGVTEDECVPVLERVSGLKSGSDFTVGYSPERINPGDKEHTFTKIKKVVAGQDAATLET
CCCCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHH
VAQVYESVVTAGVHRAASIKVAEAAKVIENTQRDLNIALMNELAIIFGRLGIDTNDVLEA
HHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHH
AGTKWNFLKFRPGLVGGHCIGVDPYYLTHKAEKIGYIPQVILAGRRINDGMGKYIAQQAV
CCCCEEEEEECCCCCCCEEECCCCCEEECCHHHHCCCHHHHHCCCCCCCHHHHHHHHHHH
KGMIHAGHCVRGCTVTVLGLTFKEDCPDLRNSKVIDIIRELEDYGVEVQVHDPLADAGEA
HHHHHCCCHHCCCEEEEEEEEEHHCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCC
SHEYGVRLVPFGELRPAAAVVAAVAHRDYRELSVEGVKGLMGDGPVLVDVKGIYDPAAVR
CHHCCEEEEECCCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHH
AAGIRLWRL
HHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-acetyl-D-mannosamine; NAD [C]

Specific reaction: UDP-N-acetyl-D-mannosamine + NAD = UDP-N-acetyl-D-mannosaminuronic-acid + NADH + Proton [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]