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The map label for this gene is zraS [H]

Identifier: 78223200

GI number: 78223200

Start: 2224582

End: 2226672

Strand: Reverse

Name: zraS [H]

Synonym: Gmet_1993

Alternate gene names: 78223200

Gene position: 2226672-2224582 (Counterclockwise)

Preceding gene: 78223201

Following gene: 78223199

Centisome position: 55.7

GC content: 57.39

Gene sequence:

>2091_bases
ATGATTCAGATCGTCCTTTCATCGATAGCTCTGCTCCTGGCTGCGGTCTACGTTTCGTGTACGCTCTGGCGGAGCGGGCG
CACCATCGCCGCTTATCTGTCCGTGGTGGCCATTGCGGTCTCGGCGTCCGTTGAATTCTGCGATTTGGCCGCAGTGGCAG
GGTACTTCGGTGATCCTCTCGATTGGAAGCGTTACAGCCTCATTGCCGAAGGGGTTGTGCCGGCGGCATGGCTCGCCTTC
AGCCTCACCCATGCCCGCACCGTGGCTGTTCGGTTCGTCACGATCGTCCAGAGGATTTTCCTGACGGCAAGCCTGATATT
TCCCGCCGCTGTCCTCGCACTCCCGCGCTCAATGTTTTTCTACTCGCCCGACTTTGATTCCGAGCGGATACTTTTCCTCT
CAAACGCCGGATATGTCTTCTACTGCGGGCTCCTGGTCTACGTTGTGATCTCGCTTATCAATCTGGAGGCCACGTTCAGC
AACGCTTCGCTTTCGTCGCGGTGGAAGATCAAGTTCGACTTCATCGGTGCGGGAAGTTTCCTGGCCGTGCTCATATTTTA
TTACAGCCAGGGGCTCCTCTACCGGACTATAAACATGAACCTGATGCCGCTACGGTCCCTGGTCTTCATCCTTGCCTTGG
GGATGATGCTCTATTCCCTGCTGGTTCGCGGCAACGGGGTCAGGATCGCCGTCTCCAGAAGCATGGCGTACAAGTCAGTG
GTCCTCTTTGCGGTGGGTCTCTACCTGGTTGCTCTGGGGATTATGGGGGAGGGACTCCGGTATTTTGGGGAAGGACACCA
GAAGACACTTGCACTTGGGGGGGCGTTTATCGTCGGCATCTGCCTCCTGGTGGTGCTCCTTTCAGAGAGCGCAAAGCGCA
AGGTCAAGGTCTTTATCCACAAGAATTTCTACCAGAGCAAGTACGACTATCGTACCCAGTGGCTTCAGTTCACCGATCGC
CTGGCTTCGGCACGGCAGGGGGAGGAACTGATGGCGGCCATTCTTTCTGGCTACTGCGCGATATTCGCCATGGGGTGCGG
TGGACTGTTTGTTCGCAATGCCGATAGCAACGAGTTCAACTGGGCAGCCGGCCACGAACTCCCGAGCACTGGCGCCGTCC
TGAGACCGGATGATCCGGTCCTGACTCCCATGATCGAACGGTCATGGGTTATCAATCTGCGGGAGGACTCGCCCGTTTGC
CTTCCCGAGCAGGAAACCTATTTCGCCGCCAATGGCATTCAGTTCCTGGTGCCGCTTTTCGGCCCCGACAGGTTGGAGGC
CGTGCTGGTCCTCGGCAGGCCCGTCAACAAGAATGAACAGTATCTTTACGAGGACTACGACCTGATGAAGACCCTTGCCC
GTCAGGCGGCGTCGGCGCTTATGAACTACCGTCTTTCCGAACAACTGGCCCGTTCGAGGGAACTGGAAGTCATGGGCCGG
GTTTCGGCCTTCATTATCCACGACCTCAAGAATTTGGTCTACACGCTGTCGCTGACAGTGGACAACGGTCGGGAGCATAT
TGCCGACCCTGAGTTTCAGGACGACTTGCTTGACACCCTTTCGAACACGGTCAACCGCATGAAGGTGCTGATCTCGCGCC
TAAAAAACCTGCCGGAGAAACGGTCGCTGGAGCTTGAGCTGGTCGACCTCCACCGTCTCGCCGTCGACACTGCGGCCATG
GTGCCGGGAAACGGGGGGATTTCAGTCACCGGTAGCGATACTTCCGCGGAGGTGGATCGAGAGGAGATTCAGAAAGTGGT
GATGAATCTTCTGGTGAACGCCCTTGAGGCAACGGACGGCAAGGGGCCCGTGATGGTCGAGGTCGGCGCCGGAAGCGCCC
CATTCATCCGGGTCAGTGATTCCGGCTGCGGGATACCGGAAGAGTTCCTTCGGACGCAACTCTTCTCGCCGTTCAAGACC
ACCAAGAAAAAAGGGCTCGGCATTGGCCTCTATCAATGTCGGCAGATCGTCGAGGCCCACGGCGGACGAATAGACGTGGC
AAGTACCGTTGGGGTTGGTACGGTTTTCACCGTATGGTTTAACTGCGGTGATCAGGGTCGGATCGGGATAACCGAACCGG
AATTCATCTGA

Upstream 100 bases:

>100_bases
GGACGGGCCGGTGCTCGTGGATGTGAAGGGTATCTATGATCCAGCCGCGGTCCGTGCCGCTGGCATCAGGCTCTGGAGGC
TGTAGCGTTACGGGTAACGA

Downstream 100 bases:

>100_bases
GGTGCAATTGATGGAAAAGCTGCTCATAGTTGATGACAACGAGGATATCCGCCGTCAGCTTCGATGGGGGATTGGGAAGG
AGTATTCCCTTCATCTGGCC

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 696; Mature: 696

Protein sequence:

>696_residues
MIQIVLSSIALLLAAVYVSCTLWRSGRTIAAYLSVVAIAVSASVEFCDLAAVAGYFGDPLDWKRYSLIAEGVVPAAWLAF
SLTHARTVAVRFVTIVQRIFLTASLIFPAAVLALPRSMFFYSPDFDSERILFLSNAGYVFYCGLLVYVVISLINLEATFS
NASLSSRWKIKFDFIGAGSFLAVLIFYYSQGLLYRTINMNLMPLRSLVFILALGMMLYSLLVRGNGVRIAVSRSMAYKSV
VLFAVGLYLVALGIMGEGLRYFGEGHQKTLALGGAFIVGICLLVVLLSESAKRKVKVFIHKNFYQSKYDYRTQWLQFTDR
LASARQGEELMAAILSGYCAIFAMGCGGLFVRNADSNEFNWAAGHELPSTGAVLRPDDPVLTPMIERSWVINLREDSPVC
LPEQETYFAANGIQFLVPLFGPDRLEAVLVLGRPVNKNEQYLYEDYDLMKTLARQAASALMNYRLSEQLARSRELEVMGR
VSAFIIHDLKNLVYTLSLTVDNGREHIADPEFQDDLLDTLSNTVNRMKVLISRLKNLPEKRSLELELVDLHRLAVDTAAM
VPGNGGISVTGSDTSAEVDREEIQKVVMNLLVNALEATDGKGPVMVEVGAGSAPFIRVSDSGCGIPEEFLRTQLFSPFKT
TKKKGLGIGLYQCRQIVEAHGGRIDVASTVGVGTVFTVWFNCGDQGRIGITEPEFI

Sequences:

>Translated_696_residues
MIQIVLSSIALLLAAVYVSCTLWRSGRTIAAYLSVVAIAVSASVEFCDLAAVAGYFGDPLDWKRYSLIAEGVVPAAWLAF
SLTHARTVAVRFVTIVQRIFLTASLIFPAAVLALPRSMFFYSPDFDSERILFLSNAGYVFYCGLLVYVVISLINLEATFS
NASLSSRWKIKFDFIGAGSFLAVLIFYYSQGLLYRTINMNLMPLRSLVFILALGMMLYSLLVRGNGVRIAVSRSMAYKSV
VLFAVGLYLVALGIMGEGLRYFGEGHQKTLALGGAFIVGICLLVVLLSESAKRKVKVFIHKNFYQSKYDYRTQWLQFTDR
LASARQGEELMAAILSGYCAIFAMGCGGLFVRNADSNEFNWAAGHELPSTGAVLRPDDPVLTPMIERSWVINLREDSPVC
LPEQETYFAANGIQFLVPLFGPDRLEAVLVLGRPVNKNEQYLYEDYDLMKTLARQAASALMNYRLSEQLARSRELEVMGR
VSAFIIHDLKNLVYTLSLTVDNGREHIADPEFQDDLLDTLSNTVNRMKVLISRLKNLPEKRSLELELVDLHRLAVDTAAM
VPGNGGISVTGSDTSAEVDREEIQKVVMNLLVNALEATDGKGPVMVEVGAGSAPFIRVSDSGCGIPEEFLRTQLFSPFKT
TKKKGLGIGLYQCRQIVEAHGGRIDVASTVGVGTVFTVWFNCGDQGRIGITEPEFI
>Mature_696_residues
MIQIVLSSIALLLAAVYVSCTLWRSGRTIAAYLSVVAIAVSASVEFCDLAAVAGYFGDPLDWKRYSLIAEGVVPAAWLAF
SLTHARTVAVRFVTIVQRIFLTASLIFPAAVLALPRSMFFYSPDFDSERILFLSNAGYVFYCGLLVYVVISLINLEATFS
NASLSSRWKIKFDFIGAGSFLAVLIFYYSQGLLYRTINMNLMPLRSLVFILALGMMLYSLLVRGNGVRIAVSRSMAYKSV
VLFAVGLYLVALGIMGEGLRYFGEGHQKTLALGGAFIVGICLLVVLLSESAKRKVKVFIHKNFYQSKYDYRTQWLQFTDR
LASARQGEELMAAILSGYCAIFAMGCGGLFVRNADSNEFNWAAGHELPSTGAVLRPDDPVLTPMIERSWVINLREDSPVC
LPEQETYFAANGIQFLVPLFGPDRLEAVLVLGRPVNKNEQYLYEDYDLMKTLARQAASALMNYRLSEQLARSRELEVMGR
VSAFIIHDLKNLVYTLSLTVDNGREHIADPEFQDDLLDTLSNTVNRMKVLISRLKNLPEKRSLELELVDLHRLAVDTAAM
VPGNGGISVTGSDTSAEVDREEIQKVVMNLLVNALEATDGKGPVMVEVGAGSAPFIRVSDSGCGIPEEFLRTQLFSPFKT
TKKKGLGIGLYQCRQIVEAHGGRIDVASTVGVGTVFTVWFNCGDQGRIGITEPEFI

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=226, Percent_Identity=30.5309734513274, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788549, Length=228, Percent_Identity=28.9473684210526, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 76750; Mature: 76750

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQIVLSSIALLLAAVYVSCTLWRSGRTIAAYLSVVAIAVSASVEFCDLAAVAGYFGDPL
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
DWKRYSLIAEGVVPAAWLAFSLTHARTVAVRFVTIVQRIFLTASLIFPAAVLALPRSMFF
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEE
YSPDFDSERILFLSNAGYVFYCGLLVYVVISLINLEATFSNASLSSRWKIKFDFIGAGSF
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEEEECCHHH
LAVLIFYYSQGLLYRTINMNLMPLRSLVFILALGMMLYSLLVRGNGVRIAVSRSMAYKSV
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
VLFAVGLYLVALGIMGEGLRYFGEGHQKTLALGGAFIVGICLLVVLLSESAKRKVKVFIH
HHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
KNFYQSKYDYRTQWLQFTDRLASARQGEELMAAILSGYCAIFAMGCGGLFVRNADSNEFN
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
WAAGHELPSTGAVLRPDDPVLTPMIERSWVINLREDSPVCLPEQETYFAANGIQFLVPLF
EECCCCCCCCCCEECCCCCCCCHHCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEEECC
GPDRLEAVLVLGRPVNKNEQYLYEDYDLMKTLARQAASALMNYRLSEQLARSRELEVMGR
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAFIIHDLKNLVYTLSLTVDNGREHIADPEFQDDLLDTLSNTVNRMKVLISRLKNLPEK
HHHHHHHHHHHHEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RSLELELVDLHRLAVDTAAMVPGNGGISVTGSDTSAEVDREEIQKVVMNLLVNALEATDG
HCCEEEEEHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
KGPVMVEVGAGSAPFIRVSDSGCGIPEEFLRTQLFSPFKTTKKKGLGIGLYQCRQIVEAH
CCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHC
GGRIDVASTVGVGTVFTVWFNCGDQGRIGITEPEFI
CCEEEEHHHCCCCEEEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MIQIVLSSIALLLAAVYVSCTLWRSGRTIAAYLSVVAIAVSASVEFCDLAAVAGYFGDPL
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
DWKRYSLIAEGVVPAAWLAFSLTHARTVAVRFVTIVQRIFLTASLIFPAAVLALPRSMFF
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHEE
YSPDFDSERILFLSNAGYVFYCGLLVYVVISLINLEATFSNASLSSRWKIKFDFIGAGSF
ECCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEEEEECCHHH
LAVLIFYYSQGLLYRTINMNLMPLRSLVFILALGMMLYSLLVRGNGVRIAVSRSMAYKSV
HHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHH
VLFAVGLYLVALGIMGEGLRYFGEGHQKTLALGGAFIVGICLLVVLLSESAKRKVKVFIH
HHHHHHHHHHHHHHHHCCHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
KNFYQSKYDYRTQWLQFTDRLASARQGEELMAAILSGYCAIFAMGCGGLFVRNADSNEFN
CCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
WAAGHELPSTGAVLRPDDPVLTPMIERSWVINLREDSPVCLPEQETYFAANGIQFLVPLF
EECCCCCCCCCCEECCCCCCCCHHCCCCEEEEECCCCCCCCCCCCCEEEECCCEEEEECC
GPDRLEAVLVLGRPVNKNEQYLYEDYDLMKTLARQAASALMNYRLSEQLARSRELEVMGR
CCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VSAFIIHDLKNLVYTLSLTVDNGREHIADPEFQDDLLDTLSNTVNRMKVLISRLKNLPEK
HHHHHHHHHHHHEEEEEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
RSLELELVDLHRLAVDTAAMVPGNGGISVTGSDTSAEVDREEIQKVVMNLLVNALEATDG
HCCEEEEEHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
KGPVMVEVGAGSAPFIRVSDSGCGIPEEFLRTQLFSPFKTTKKKGLGIGLYQCRQIVEAH
CCCEEEEECCCCCCEEEECCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHC
GGRIDVASTVGVGTVFTVWFNCGDQGRIGITEPEFI
CCEEEEHHHCCCCEEEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 2666400; 11243806 [H]