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The map label for this gene is birA [H]

Identifier: 78223194

GI number: 78223194

Start: 2216811

End: 2217827

Strand: Reverse

Name: birA [H]

Synonym: Gmet_1987

Alternate gene names: 78223194

Gene position: 2217827-2216811 (Counterclockwise)

Preceding gene: 78223195

Following gene: 78223193

Centisome position: 55.48

GC content: 59.1

Gene sequence:

>1017_bases
GTGAGCGGAGAGTTGCCACGCCCCGACGCAATCGGCAGCAAGGAGATCGACCGGAAGATTCTGGAAATCTTCAGGAGCCG
TGACGGGGGCGTTGTTTCCGGCGAGGAATTGAGCCGGTCTCTGGATGTATCACGCACGGCTGTCTGGAAGCATATCAAGA
CCCTTAATGGGCTCGGCTACCGCATCGAGGCAGTCCCCTCCCGGGGGTATCGTCTCGTATCAACTCCCGATATCCTCACC
CCCGCAGAACTGTCGGTCGGACTTAACGTAGCCAGGATCGGCACATCTCTTGTTTTTGTCGGCGAAACTGACTCAACTAA
CACCCTTGCCTATCGTCTTGCCGAAGATGGTGCCGTCGAAGGAACCGTGGTCATCGCCGACGCACAGAACCGCGGCAGGG
GGCGGCTCGGGAGGCAGTGGGAGTCGCCCGCCGGGGTCAATCTCTATTGTTCCATTATTCTTCGCCCGCCCATAATGCCT
CTTCACGCGCCCCAGCTCACCTTCCTGTCGGCCGTTGCGGTGGCAGAGGCCATTGAGCGTTCTGCCGGGCTTGCACCCGT
CATCAAATGGCCCAATGATGTACTCGTGAACGGTTTCAAGGTTGCCGGCATGCTCAACGAGATGAGCGCCGAAACCGAAC
GGATCGATTTCCTTGTCCTTGGCATCGGGGTGAATATCAATATGCAGCGGGATCAGTTCCCGACCGATCTGCGCCATCCC
GCAAGTTCCCTGGCCATTGAGGCGGGGCATGAGGTGTCCAGGCTCTCATTTGCCCGGGCGCTTCTCGAGTCTCTTGATGT
GCATTACTCACAGTACCGTTCAGAGGGTTACGCTCCGCTCCGCCAGGCATGGCTTGGCCGGAGCGCCGTCATGGGAAGAA
GGGTCCGGGTAAGCGGCAATCAGGGGGATATGACGGGGACTGTCGAGGGGATTGACGAGATCGGCGCTCTGTTGCTGAGA
ACCGCTCATGGCACCCTTGAGCGGGTTCTGGCAGGGGATGTGACAATTGAAGGGTAA

Upstream 100 bases:

>100_bases
CAGTCAGAGGAATTGCCGAGGCAGGGGTTGACATCATTTCCGTGGGGGCGCTTACCCATTCTGCCCGAGCCATGGACATC
TCGATGCTTCTGGAGGCTGA

Downstream 100 bases:

>100_bases
GGTGGCGCCGTGCTTTCTGTTCTGGAGGTAAACCCATGCTTCTCGTCATAGACGTGGGCAACACGAATATCGTTCTCGGC
ATCTATGACGGTCAGAGGCT

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 338; Mature: 337

Protein sequence:

>338_residues
MSGELPRPDAIGSKEIDRKILEIFRSRDGGVVSGEELSRSLDVSRTAVWKHIKTLNGLGYRIEAVPSRGYRLVSTPDILT
PAELSVGLNVARIGTSLVFVGETDSTNTLAYRLAEDGAVEGTVVIADAQNRGRGRLGRQWESPAGVNLYCSIILRPPIMP
LHAPQLTFLSAVAVAEAIERSAGLAPVIKWPNDVLVNGFKVAGMLNEMSAETERIDFLVLGIGVNINMQRDQFPTDLRHP
ASSLAIEAGHEVSRLSFARALLESLDVHYSQYRSEGYAPLRQAWLGRSAVMGRRVRVSGNQGDMTGTVEGIDEIGALLLR
TAHGTLERVLAGDVTIEG

Sequences:

>Translated_338_residues
MSGELPRPDAIGSKEIDRKILEIFRSRDGGVVSGEELSRSLDVSRTAVWKHIKTLNGLGYRIEAVPSRGYRLVSTPDILT
PAELSVGLNVARIGTSLVFVGETDSTNTLAYRLAEDGAVEGTVVIADAQNRGRGRLGRQWESPAGVNLYCSIILRPPIMP
LHAPQLTFLSAVAVAEAIERSAGLAPVIKWPNDVLVNGFKVAGMLNEMSAETERIDFLVLGIGVNINMQRDQFPTDLRHP
ASSLAIEAGHEVSRLSFARALLESLDVHYSQYRSEGYAPLRQAWLGRSAVMGRRVRVSGNQGDMTGTVEGIDEIGALLLR
TAHGTLERVLAGDVTIEG
>Mature_337_residues
SGELPRPDAIGSKEIDRKILEIFRSRDGGVVSGEELSRSLDVSRTAVWKHIKTLNGLGYRIEAVPSRGYRLVSTPDILTP
AELSVGLNVARIGTSLVFVGETDSTNTLAYRLAEDGAVEGTVVIADAQNRGRGRLGRQWESPAGVNLYCSIILRPPIMPL
HAPQLTFLSAVAVAEAIERSAGLAPVIKWPNDVLVNGFKVAGMLNEMSAETERIDFLVLGIGVNINMQRDQFPTDLRHPA
SSLAIEAGHEVSRLSFARALLESLDVHYSQYRSEGYAPLRQAWLGRSAVMGRRVRVSGNQGDMTGTVEGIDEIGALLLRT
AHGTLERVLAGDVTIEG

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=310, Percent_Identity=31.2903225806452, Blast_Score=135, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 36575; Mature: 36444

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGELPRPDAIGSKEIDRKILEIFRSRDGGVVSGEELSRSLDVSRTAVWKHIKTLNGLGY
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHCHHHHHHHHHHHHHCCCCE
RIEAVPSRGYRLVSTPDILTPAELSVGLNVARIGTSLVFVGETDSTNTLAYRLAEDGAVE
EEEECCCCCEEEECCCCCCCCCHHHHCCEEEEECCEEEEEECCCCCCEEEEEEECCCCCC
GTVVIADAQNRGRGRLGRQWESPAGVNLYCSIILRPPIMPLHAPQLTFLSAVAVAEAIER
CEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
SAGLAPVIKWPNDVLVNGFKVAGMLNEMSAETERIDFLVLGIGVNINMQRDQFPTDLRHP
HCCCCEEEECCCHHHCCCCCEEHHHHHHCCCHHHEEEEEEEECEEEEECCCCCCCHHHCC
ASSLAIEAGHEVSRLSFARALLESLDVHYSQYRSEGYAPLRQAWLGRSAVMGRRVRVSGN
HHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCEEEEECC
QGDMTGTVEGIDEIGALLLRTAHGTLERVLAGDVTIEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC
>Mature Secondary Structure 
SGELPRPDAIGSKEIDRKILEIFRSRDGGVVSGEELSRSLDVSRTAVWKHIKTLNGLGY
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHHCHHHHHHHHHHHHHCCCCE
RIEAVPSRGYRLVSTPDILTPAELSVGLNVARIGTSLVFVGETDSTNTLAYRLAEDGAVE
EEEECCCCCEEEECCCCCCCCCHHHHCCEEEEECCEEEEEECCCCCCEEEEEEECCCCCC
GTVVIADAQNRGRGRLGRQWESPAGVNLYCSIILRPPIMPLHAPQLTFLSAVAVAEAIER
CEEEEEECCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
SAGLAPVIKWPNDVLVNGFKVAGMLNEMSAETERIDFLVLGIGVNINMQRDQFPTDLRHP
HCCCCEEEECCCHHHCCCCCEEHHHHHHCCCHHHEEEEEEEECEEEEECCCCCCCHHHCC
ASSLAIEAGHEVSRLSFARALLESLDVHYSQYRSEGYAPLRQAWLGRSAVMGRRVRVSGN
HHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHCCCEEEEECC
QGDMTGTVEGIDEIGALLLRTAHGTLERVLAGDVTIEG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]