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The map label for this gene is nadB [H]

Identifier: 78223161

GI number: 78223161

Start: 2180498

End: 2182093

Strand: Direct

Name: nadB [H]

Synonym: Gmet_1954

Alternate gene names: 78223161

Gene position: 2180498-2182093 (Clockwise)

Preceding gene: 78223158

Following gene: 78223162

Centisome position: 54.55

GC content: 56.64

Gene sequence:

>1596_bases
ATGAAATTAGCGACTGATTTTCTTGTTATCGGCAGCGGTATCGCCGGGCTCACCTTTGCTCTCCAGGCAGCACGACACGG
CACCGTGGCCATCGTCACCAAACGCGAAGTGACGGAATCCGCCACCAACTATGCGCAGGGGGGCATCGCCACGGTCTTTT
CCCAGGAAGACACCTTTGATGCCCATGTAGAGGATACTCTTGTGGCTGGCGCAGGCATCTGCCATGAAGATGTGGTGCGG
ATGGTGGTCGAAGAAGGCCCCAAGGTCATCCGCAACCTCATCGAGTGGGGGGTCCAGTTTACCCGCAGTGGCGAGGCATT
TGACCTGACCCGCGAGGGTGGGCACAGCCAGCGACGCATCCTCCACGCTGACGATGTCACGGGCCGCGAAATTGAGCGGG
CACTGGTGGCGGCGGTCCGCGATAACCCCAACATACAGCTCTACGAGCATCACATTGCCATTGATTTGATCACCATGTCA
AAGGTGACCCACAAGCGAGTCAAGCCGGACCGTTGCCTCGGCGCTCATGTTCTGGACATCGCTACCAACAGCGTGAAGAC
CTTCAGGGCAAAAATCACCCTTCTCGCTACCGGAGGCGCCGGCAAGGTCTACCTCTACACCTGCAACCCCGACGTGGCCA
CCGGCGACGGCGTAGCCATGGCGTACCGGGCCGGCGCTACCGTCGCCAACATGGAATTCATGCAATTTCATCCCACCACC
CTCTACCATCCCCATGCAAAATCTTTTCTCATTTCCGAAGCGGTACGGGGCGAGGGAGCCATCCTGCGCCGCCGTGACGG
AACTGCGTTCATGGAGAAATACCACAAGCTGAAGGATCTGGCCCCCCGTGACATCGTGGCGAGGGCCATCGACAATGAGA
TGAAGACCCATGGCGACGACTGTGTCTACCTGGATATCACCCACAAAGACCCGGAATACGTCCGCACCCGCTTCCCCAAT
ATCTACCAGACATGTCTGGAATTCGGATTGGACATGACTAAAGAACCGCTGCCTGTGGTTCCTGCGGCCCACTACCTTTG
CGGCGGTGTGGCGGTGGACATGAACGGTGAAACTGACCTAAGCCGTCTCTATGCTATCGGTGAGGTAGCTTTCACCGGTC
TCCACGGCGCAAATCGCCTGGCAAGCAACTCCCTCCTTGAAGCGGCAGTCTTAGCGGGACGTGCCTACAAACGGGCGATT
GAGGAACTGCAACAGAACCATTTCGAGTTTCCGGAAATCCCCGAGTGGGATGCGGGAACTGCCACCGACAGTGACGAAAT
GGTCGTCGTCTCCCAAAACTGGGACGAAATTCGGCGGTTTATGTGGAATTACGTGGGAATTGTCCGTTCGGACAAGCGTC
TCGAGCGTGCACAGCACCGCATCCGCTTGATCCAGGAAGAAATTGATGACTACTACTGGGATTTCACCATCACCTCTGAC
CTTATTGAGCTGCGAAACATCGCCACGGTGGCGGAACTGATCGTCAAATGCGCCCTGCAACGGAAGGAATCCCGCGGCCT
CCACTACACCATCGATTATCTGGAACGTGACGACGTGCACTGGAAGAAAGATACTTTTTTGAAAAAACAGTTCTGA

Upstream 100 bases:

>100_bases
CCATCCTTAAATACCTTGACAAAGGGGGGAGGCAGGAGAATAATCGGCATTCTTCATAAATACTTACACATCCTTTACCC
AATAATGAAGGAGAAGCCCC

Downstream 100 bases:

>100_bases
GGAACCTCTTGCATGACGATAGACGAACTGAGGCAGGAAATTGACCGGATGGACAGCGAGCTTCTCCGGATCTTCAACCG
GCGGGCTTCCCTGGCGCTGG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 531; Mature: 531

Protein sequence:

>531_residues
MKLATDFLVIGSGIAGLTFALQAARHGTVAIVTKREVTESATNYAQGGIATVFSQEDTFDAHVEDTLVAGAGICHEDVVR
MVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRILHADDVTGREIERALVAAVRDNPNIQLYEHHIAIDLITMS
KVTHKRVKPDRCLGAHVLDIATNSVKTFRAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATVANMEFMQFHPTT
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGDDCVYLDITHKDPEYVRTRFPN
IYQTCLEFGLDMTKEPLPVVPAAHYLCGGVAVDMNGETDLSRLYAIGEVAFTGLHGANRLASNSLLEAAVLAGRAYKRAI
EELQQNHFEFPEIPEWDAGTATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERAQHRIRLIQEEIDDYYWDFTITSD
LIELRNIATVAELIVKCALQRKESRGLHYTIDYLERDDVHWKKDTFLKKQF

Sequences:

>Translated_531_residues
MKLATDFLVIGSGIAGLTFALQAARHGTVAIVTKREVTESATNYAQGGIATVFSQEDTFDAHVEDTLVAGAGICHEDVVR
MVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRILHADDVTGREIERALVAAVRDNPNIQLYEHHIAIDLITMS
KVTHKRVKPDRCLGAHVLDIATNSVKTFRAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATVANMEFMQFHPTT
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGDDCVYLDITHKDPEYVRTRFPN
IYQTCLEFGLDMTKEPLPVVPAAHYLCGGVAVDMNGETDLSRLYAIGEVAFTGLHGANRLASNSLLEAAVLAGRAYKRAI
EELQQNHFEFPEIPEWDAGTATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERAQHRIRLIQEEIDDYYWDFTITSD
LIELRNIATVAELIVKCALQRKESRGLHYTIDYLERDDVHWKKDTFLKKQF
>Mature_531_residues
MKLATDFLVIGSGIAGLTFALQAARHGTVAIVTKREVTESATNYAQGGIATVFSQEDTFDAHVEDTLVAGAGICHEDVVR
MVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRILHADDVTGREIERALVAAVRDNPNIQLYEHHIAIDLITMS
KVTHKRVKPDRCLGAHVLDIATNSVKTFRAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATVANMEFMQFHPTT
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGDDCVYLDITHKDPEYVRTRFPN
IYQTCLEFGLDMTKEPLPVVPAAHYLCGGVAVDMNGETDLSRLYAIGEVAFTGLHGANRLASNSLLEAAVLAGRAYKRAI
EELQQNHFEFPEIPEWDAGTATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERAQHRIRLIQEEIDDYYWDFTITSD
LIELRNIATVAELIVKCALQRKESRGLHYTIDYLERDDVHWKKDTFLKKQF

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=536, Percent_Identity=31.9029850746269, Blast_Score=221, Evalue=2e-57,
Organism=Escherichia coli, GI1788928, Length=517, Percent_Identity=53.57833655706, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1790597, Length=548, Percent_Identity=31.7518248175182, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1786942, Length=567, Percent_Identity=31.7460317460317, Blast_Score=214, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI17505833, Length=535, Percent_Identity=31.9626168224299, Blast_Score=215, Evalue=5e-56,
Organism=Caenorhabditis elegans, GI17550100, Length=564, Percent_Identity=30.6737588652482, Blast_Score=211, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI71986328, Length=435, Percent_Identity=25.0574712643678, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322701, Length=543, Percent_Identity=32.2283609576427, Blast_Score=230, Evalue=3e-61,
Organism=Saccharomyces cerevisiae, GI6322416, Length=548, Percent_Identity=30.8394160583942, Blast_Score=228, Evalue=3e-60,
Organism=Drosophila melanogaster, GI17137288, Length=557, Percent_Identity=30.3411131059246, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24655642, Length=557, Percent_Identity=30.3411131059246, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24655647, Length=557, Percent_Identity=30.3411131059246, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24663005, Length=535, Percent_Identity=29.5327102803738, Blast_Score=193, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59683; Mature: 59683

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLATDFLVIGSGIAGLTFALQAARHGTVAIVTKREVTESATNYAQGGIATVFSQEDTFD
CCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCCC
AHVEDTLVAGAGICHEDVVRMVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRI
CHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCEECCCCCEEEECCCCCCCCCEE
LHADDVTGREIERALVAAVRDNPNIQLYEHHIAIDLITMSKVTHKRVKPDRCLGAHVLDI
EECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEHHHHHHHCCCCCHHHCCHHEEH
ATNSVKTFRAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATVANMEFMQFHPTT
HHCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCEECEEEEEECCCE
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGDD
EECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
CVYLDITHKDPEYVRTRFPNIYQTCLEFGLDMTKEPLPVVPAAHYLCGGVAVDMNGETDL
EEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEECCCCCHH
SRLYAIGEVAFTGLHGANRLASNSLLEAAVLAGRAYKRAIEELQQNHFEFPEIPEWDAGT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
ATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERAQHRIRLIQEEIDDYYWDFTITSD
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEEEHHH
LIELRNIATVAELIVKCALQRKESRGLHYTIDYLERDDVHWKKDTFLKKQF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MKLATDFLVIGSGIAGLTFALQAARHGTVAIVTKREVTESATNYAQGGIATVFSQEDTFD
CCCCCCEEEECCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHCCCCEEEEECCCCCCC
AHVEDTLVAGAGICHEDVVRMVVEEGPKVIRNLIEWGVQFTRSGEAFDLTREGGHSQRRI
CHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHCCEECCCCCEEEECCCCCCCCCEE
LHADDVTGREIERALVAAVRDNPNIQLYEHHIAIDLITMSKVTHKRVKPDRCLGAHVLDI
EECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEEHHHHHHHCCCCCHHHCCHHEEH
ATNSVKTFRAKITLLATGGAGKVYLYTCNPDVATGDGVAMAYRAGATVANMEFMQFHPTT
HHCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEEEEECCCCEECEEEEEECCCE
LYHPHAKSFLISEAVRGEGAILRRRDGTAFMEKYHKLKDLAPRDIVARAIDNEMKTHGDD
EECCCHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCC
CVYLDITHKDPEYVRTRFPNIYQTCLEFGLDMTKEPLPVVPAAHYLCGGVAVDMNGETDL
EEEEEEECCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCEEEECCCCCHH
SRLYAIGEVAFTGLHGANRLASNSLLEAAVLAGRAYKRAIEELQQNHFEFPEIPEWDAGT
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
ATDSDEMVVVSQNWDEIRRFMWNYVGIVRSDKRLERAQHRIRLIQEEIDDYYWDFTITSD
CCCCCCEEEEECCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHEEEEEEHHH
LIELRNIATVAELIVKCALQRKESRGLHYTIDYLERDDVHWKKDTFLKKQF
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]