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The map label for this gene is yjbJ [H]

Identifier: 78223160

GI number: 78223160

Start: 2179681

End: 2180292

Strand: Reverse

Name: yjbJ [H]

Synonym: Gmet_1953

Alternate gene names: 78223160

Gene position: 2180292-2179681 (Counterclockwise)

Preceding gene: 78223164

Following gene: 78223159

Centisome position: 54.54

GC content: 56.05

Gene sequence:

>612_bases
ATGCCGAAAGTTGCCAGTATTCTGGCTCTTGCCCTCTTGCTCCTGGGGGGAGTGGGGTACTTTGACGCCAACGCAGACAT
TTACCGGTACGAAGATGAAGAGGGGGTCGTTCATTTTACCGACGCCCCCACCGATAAACGGTTCAAGATTTTCATGAGGG
ATATCAAGCGGGACCGGAAGCTTCGCACCACGTTTAAGCTTGCACGCTTTACCCGTGACCCTGCCGAATTCGAGCCGATC
ATCACCCAGTGCGCCCAGGAATTCGGCGTTGACAGATCGCTGGTGAAGGCTGTAATCCATGCCGAATCGGGATACAACCC
CAATGCCGTCTCCCCCAAGGGGGCAAGTGGGCTCATGCAGCTCATGCCCAAGACGGCCCGGGATCTGAAAGTGGCTAACA
GCCTCGACCCGCGGGAGAACATTCGCGGTGGTGTCCGCTACCTTCGCTTCCTGCTCGATACGTTCCGGGGTGATGAGAGC
TTGGCCCTGGCAGCCTATAATGCCGGACTTTCACGGGTAGCCCAGTATAACGGCATTCCCCCTTATCAGGAAACGCGCAC
CTATGTCGACAGGGTGCTTACTTACAGAAAATCCTACCAGACCACCAATTGA

Upstream 100 bases:

>100_bases
ACCTTTGAGATGACCGTTGGGGTTGCATTGCCTTCTGAGTTGGTCTATATTTTGAATGGCTTTCGGCAGGGACGCCGATC
AGCGCTGTTTGGGAGGAAAC

Downstream 100 bases:

>100_bases
GAATCAGCGTACATGCCTGACACTAACGCCAAGACCATCTGGAATATCCCGAACATTCTCACTCTCCTGCGAATCGCTCT
GATACCCGTTTTGGCGGTGC

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MPKVASILALALLLLGGVGYFDANADIYRYEDEEGVVHFTDAPTDKRFKIFMRDIKRDRKLRTTFKLARFTRDPAEFEPI
ITQCAQEFGVDRSLVKAVIHAESGYNPNAVSPKGASGLMQLMPKTARDLKVANSLDPRENIRGGVRYLRFLLDTFRGDES
LALAAYNAGLSRVAQYNGIPPYQETRTYVDRVLTYRKSYQTTN

Sequences:

>Translated_203_residues
MPKVASILALALLLLGGVGYFDANADIYRYEDEEGVVHFTDAPTDKRFKIFMRDIKRDRKLRTTFKLARFTRDPAEFEPI
ITQCAQEFGVDRSLVKAVIHAESGYNPNAVSPKGASGLMQLMPKTARDLKVANSLDPRENIRGGVRYLRFLLDTFRGDES
LALAAYNAGLSRVAQYNGIPPYQETRTYVDRVLTYRKSYQTTN
>Mature_202_residues
PKVASILALALLLLGGVGYFDANADIYRYEDEEGVVHFTDAPTDKRFKIFMRDIKRDRKLRTTFKLARFTRDPAEFEPII
TQCAQEFGVDRSLVKAVIHAESGYNPNAVSPKGASGLMQLMPKTARDLKVANSLDPRENIRGGVRYLRFLLDTFRGDESL
ALAAYNAGLSRVAQYNGIPPYQETRTYVDRVLTYRKSYQTTN

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=133, Percent_Identity=36.0902255639098, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 22952; Mature: 22820

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKVASILALALLLLGGVGYFDANADIYRYEDEEGVVHFTDAPTDKRFKIFMRDIKRDRK
CCHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHH
LRTTFKLARFTRDPAEFEPIITQCAQEFGVDRSLVKAVIHAESGYNPNAVSPKGASGLMQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LMPKTARDLKVANSLDPRENIRGGVRYLRFLLDTFRGDESLALAAYNAGLSRVAQYNGIP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCC
PYQETRTYVDRVLTYRKSYQTTN
CHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PKVASILALALLLLGGVGYFDANADIYRYEDEEGVVHFTDAPTDKRFKIFMRDIKRDRK
CHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHH
LRTTFKLARFTRDPAEFEPIITQCAQEFGVDRSLVKAVIHAESGYNPNAVSPKGASGLMQ
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
LMPKTARDLKVANSLDPRENIRGGVRYLRFLLDTFRGDESLALAAYNAGLSRVAQYNGIP
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCC
PYQETRTYVDRVLTYRKSYQTTN
CHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]