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The map label for this gene is pgk

Identifier: 78223154

GI number: 78223154

Start: 2175397

End: 2176593

Strand: Direct

Name: pgk

Synonym: Gmet_1947

Alternate gene names: 78223154

Gene position: 2175397-2176593 (Clockwise)

Preceding gene: 78223153

Following gene: 78223155

Centisome position: 54.42

GC content: 54.89

Gene sequence:

>1197_bases
ATGGCAATCCGCTATATCGACGAAATCGAAAGTCTCAAGGGGAAAAAGGTATTTATCAGGGTCGACTTCAACGTTCCTCT
GGATGAACATCAGAATATAACCGAAGACACCCGGATCCGGGCCGTCCTTCCCACTATCAACTTTGCCCTTGATGCGGGCG
CCAAGGTAATTCTTGCATCCCACTTGGGTCGCCCAAAGGGTGAGCGAAAACCGAAGTATTCCATGGCTCCGGCGGCCAAG
CGCCTCTCGCGCCTGCTCAACAAGGAAGTGCAACTGGCTCCCGACTGCATCGGCGATGAAGTCAAGAAGATGATCGACGC
CATGAAACCGGGCGAAGTCCTTCTACTCGAAAACGTCCGTTTCTACGAGGGCGAAGAGAAAAATGACGCCGATTTCGCCA
AGGCCCTGGCCAACGACTGTGAAATATACATCAACGACGCCTTTGCCGTCTCCCACAGGGCCCACGCATCCGTCGAAGCC
ATCACGAAGTTTTTCCCGGTCGTCGCTGCCGGTTTCCTCATGAACAATGAGATTAATTATTTCGAGAAGGCGATGCAAAA
GCCCATCAGGCCCTTGGTCGCCATTCTCGGCGGGGCCAAGGTTTCCGGAAAACTTGAGGTATTGGAAAGCCTGGTCAACA
AGGTAGACAAGATTATCATTGGCGGCGGCATGGCCTTCACGTTCCTCAAAGCCCTCGGCTATAACGTCGGCAAATCGCTG
GTGGAGGAAGAACTGCTGGAAATCGCCCTCACTACCTACACCAAGGCCCGTGAAAAAGGCGTGAAATTCTATCTACCGGT
CGACTGCGTTGCGGCCGACCGCTTCAACCCCGAGGCTGAAACCAAAGTGACGACCATTCAGGAAATCCCCGAGGAGTGGA
TGGCACTCGACATCGGACCGGCAACCGTGACCCTCTTCACGGAAGCGCTCCAGAACGCCAAAACCATTATCTGGAATGGT
CCCATGGGCGTATTCGAGATGGATGCCTTCTCCCGCGGAACCTTTGCCATGGTTTCGGCCGTGGCCAACTCCTATGCTCT
CACCATCGTCGGAGGAGGAGACACCGATTCAGCCGTCCATCGCGCGGGGGAATACGCGAAGATCAGCTACATTTCCACAG
GCGGCGGCGCGTTCCTGGAACTCCTCGAAGGGAAGCATCTTCCCGGCATCAAAGTGCTGGAAGAAAACGGCAAGTAG

Upstream 100 bases:

>100_bases
CCCACAGGGGGGATTATTCCCCCCTTTTTTTGTCTTCAGCCCGGCATCCGTTGCCTCTGGCACATGGTTTTCATTATCAC
AACGGTTTCAGGAGGGTACC

Downstream 100 bases:

>100_bases
ATAACAAACGGGGTGACGACATGAGAACACCGATTATTGCTGGCAATTGGAAACTCTTCAAAAAGAGCAGCGAAGCACAA
GACTTCGTTGCCGAACTGAT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAIRYIDEIESLKGKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVILASHLGRPKGERKPKYSMAPAAK
RLSRLLNKEVQLAPDCIGDEVKKMIDAMKPGEVLLLENVRFYEGEEKNDADFAKALANDCEIYINDAFAVSHRAHASVEA
ITKFFPVVAAGFLMNNEINYFEKAMQKPIRPLVAILGGAKVSGKLEVLESLVNKVDKIIIGGGMAFTFLKALGYNVGKSL
VEEELLEIALTTYTKAREKGVKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGPATVTLFTEALQNAKTIIWNG
PMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDSAVHRAGEYAKISYISTGGGAFLELLEGKHLPGIKVLEENGK

Sequences:

>Translated_398_residues
MAIRYIDEIESLKGKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVILASHLGRPKGERKPKYSMAPAAK
RLSRLLNKEVQLAPDCIGDEVKKMIDAMKPGEVLLLENVRFYEGEEKNDADFAKALANDCEIYINDAFAVSHRAHASVEA
ITKFFPVVAAGFLMNNEINYFEKAMQKPIRPLVAILGGAKVSGKLEVLESLVNKVDKIIIGGGMAFTFLKALGYNVGKSL
VEEELLEIALTTYTKAREKGVKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGPATVTLFTEALQNAKTIIWNG
PMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDSAVHRAGEYAKISYISTGGGAFLELLEGKHLPGIKVLEENGK
>Mature_397_residues
AIRYIDEIESLKGKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVILASHLGRPKGERKPKYSMAPAAKR
LSRLLNKEVQLAPDCIGDEVKKMIDAMKPGEVLLLENVRFYEGEEKNDADFAKALANDCEIYINDAFAVSHRAHASVEAI
TKFFPVVAAGFLMNNEINYFEKAMQKPIRPLVAILGGAKVSGKLEVLESLVNKVDKIIIGGGMAFTFLKALGYNVGKSLV
EEELLEIALTTYTKAREKGVKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGPATVTLFTEALQNAKTIIWNGP
MGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDSAVHRAGEYAKISYISTGGGAFLELLEGKHLPGIKVLEENGK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=46.3414634146341, Blast_Score=348, Evalue=4e-96,
Organism=Homo sapiens, GI31543397, Length=410, Percent_Identity=43.6585365853659, Blast_Score=338, Evalue=5e-93,
Organism=Escherichia coli, GI1789294, Length=390, Percent_Identity=45.1282051282051, Blast_Score=317, Evalue=8e-88,
Organism=Caenorhabditis elegans, GI17508823, Length=409, Percent_Identity=46.4547677261614, Blast_Score=352, Evalue=2e-97,
Organism=Saccharomyces cerevisiae, GI6319857, Length=404, Percent_Identity=48.7623762376238, Blast_Score=354, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24581203, Length=404, Percent_Identity=45.7920792079208, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI17136394, Length=403, Percent_Identity=45.1612903225806, Blast_Score=323, Evalue=2e-88,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_GEOMG (Q39U98)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384901.1
- ProteinModelPortal:   Q39U98
- SMR:   Q39U98
- STRING:   Q39U98
- GeneID:   3741598
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1947
- NMPDR:   fig|269799.3.peg.839
- eggNOG:   COG0126
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- PhylomeDB:   Q39U98
- ProtClustDB:   PRK00073
- BioCyc:   GMET269799:GMET_1947-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 43579; Mature: 43448

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 120-120 BINDING 153-153 BINDING 204-204 BINDING 326-326

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIRYIDEIESLKGKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVILAS
CCCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHCCCCCEEHHH
HLGRPKGERKPKYSMAPAAKRLSRLLNKEVQLAPDCIGDEVKKMIDAMKPGEVLLLENVR
HCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCHHCCHHHHHHHHHCCCCCEEEEECEE
FYEGEEKNDADFAKALANDCEIYINDAFAVSHRAHASVEAITKFFPVVAAGFLMNNEINY
EECCCCCCHHHHHHHHHCCCEEEEECCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FEKAMQKPIRPLVAILGGAKVSGKLEVLESLVNKVDKIIIGGGMAFTFLKALGYNVGKSL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHH
VEEELLEIALTTYTKAREKGVKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGP
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHCCHHHHEEECCC
ATVTLFTEALQNAKTIIWNGPMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDSAVH
HHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCHHHHH
RAGEYAKISYISTGGGAFLELLEGKHLPGIKVLEENGK
HCCCCEEEEEEECCCHHHHHHHCCCCCCCCEEECCCCC
>Mature Secondary Structure 
AIRYIDEIESLKGKKVFIRVDFNVPLDEHQNITEDTRIRAVLPTINFALDAGAKVILAS
CCCHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECHHHHHHCCCCCEEHHH
HLGRPKGERKPKYSMAPAAKRLSRLLNKEVQLAPDCIGDEVKKMIDAMKPGEVLLLENVR
HCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHCCCHHCCHHHHHHHHHCCCCCEEEEECEE
FYEGEEKNDADFAKALANDCEIYINDAFAVSHRAHASVEAITKFFPVVAAGFLMNNEINY
EECCCCCCHHHHHHHHHCCCEEEEECCEEECHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
FEKAMQKPIRPLVAILGGAKVSGKLEVLESLVNKVDKIIIGGGMAFTFLKALGYNVGKSL
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHHH
VEEELLEIALTTYTKAREKGVKFYLPVDCVAADRFNPEAETKVTTIQEIPEEWMALDIGP
HHHHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCCCCHHHHHHHHHCCHHHHEEECCC
ATVTLFTEALQNAKTIIWNGPMGVFEMDAFSRGTFAMVSAVANSYALTIVGGGDTDSAVH
HHHHHHHHHHHCCCEEEECCCCCEEEECCCCCCHHHHHHHHHCCEEEEEEECCCCHHHHH
RAGEYAKISYISTGGGAFLELLEGKHLPGIKVLEENGK
HCCCCEEEEEEECCCHHHHHHHCCCCCCCCEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA