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The map label for this gene is gap [H]

Identifier: 78223153

GI number: 78223153

Start: 2174289

End: 2175284

Strand: Direct

Name: gap [H]

Synonym: Gmet_1946

Alternate gene names: 78223153

Gene position: 2174289-2175284 (Clockwise)

Preceding gene: 78223135

Following gene: 78223154

Centisome position: 54.39

GC content: 54.92

Gene sequence:

>996_bases
ATGGCTCTAAGGGTTGCAATCAACGGCTTCGGTAGAATCGGGCGTTCCGTACTCCGTGCGGCAATCAAGGAGAAAGGTAT
CGAGTTTGTCGCCATCAACGACCTCACCGATGCCAAGACCCTAGCACACCTGCTCAAGTATGACTCTGTTCACGGACCGT
TTCCCGGCAAAGTCGAAGCCGGCAATGGTGAAATCATAGTCAACGGCAAACCGATCAAGATCCTTGCCGTAAGGAATCCT
GAGGAACTCCCCTGGAAGAAGGAAAAGATCGACGTGGTCCTCGAGTCAACCGGTCTGTTCACCTCCCGTGAAAAGGCCGA
ACTTCACCTGAAAGCAGGCGCAAAAAAAATTATCATCTCCGCTCCTGCCACCAACGAGGACATCACCATCGTCATGGGTG
TCAACAGCAACCTCTATGACCCCAAGAAGCACAACATCATCTCAAATGCTTCCTGCACCACCAACTGCCTGGCGCCGGTT
GCCAAGGTGTTGCACGAAACCTTCGGCATCGAAAAAGGCCTGGTGACGACGGTTCACTCCTACACCAACGACCAGCAGAT
CCTTGACCTTCCCCATAAAGACCTGCGCCGGGCCCGCGCGGCCGCCATGTCCATGATTCCGACCTCCACTGGCGCTGCCA
AGGCAGTCGCCCTTGTGCTGCCCGAGTTGAAGGGGAAACTGGATGGCATGGCAATTCGCGTCCCGACCCCCAACGTCTCG
GTCGTTGACCTCGTTGTTACCCTTTCCAAGAAGGCAGATGCCGACAAAGTAAACGCGGCCCTGAAAAAGGCCTCCAAGGG
TTCCCTCAAGGGTGTTCTGGGCTTTTCGGAGGAACCTCTCGTCTCCATCGACTTCAATGGCAATCCCCTCTCCTCCATCG
TTGATGCCGGCTGCACCAAGGTGATCGAGGGGAACATGGTCAAGGTTCTTTCCTGGTATGACAACGAAACAGGCTTCTCC
CACCGCGTCGTTGATCTCATGAAACTCATCGCATAA

Upstream 100 bases:

>100_bases
GGACAAAAAGGTTGACTCGTAACAAATTACTTTATTATGATGTCAAAGTTTACAATTAAAGCCGTCCAAGGCTAACATAT
CAAACCAACAGGAGGGTTCA

Downstream 100 bases:

>100_bases
CAATTACTCAAACCCACAGGGGGGATTATTCCCCCCTTTTTTTGTCTTCAGCCCGGCATCCGTTGCCTCTGGCACATGGT
TTTCATTATCACAACGGTTT

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MALRVAINGFGRIGRSVLRAAIKEKGIEFVAINDLTDAKTLAHLLKYDSVHGPFPGKVEAGNGEIIVNGKPIKILAVRNP
EELPWKKEKIDVVLESTGLFTSREKAELHLKAGAKKIIISAPATNEDITIVMGVNSNLYDPKKHNIISNASCTTNCLAPV
AKVLHETFGIEKGLVTTVHSYTNDQQILDLPHKDLRRARAAAMSMIPTSTGAAKAVALVLPELKGKLDGMAIRVPTPNVS
VVDLVVTLSKKADADKVNAALKKASKGSLKGVLGFSEEPLVSIDFNGNPLSSIVDAGCTKVIEGNMVKVLSWYDNETGFS
HRVVDLMKLIA

Sequences:

>Translated_331_residues
MALRVAINGFGRIGRSVLRAAIKEKGIEFVAINDLTDAKTLAHLLKYDSVHGPFPGKVEAGNGEIIVNGKPIKILAVRNP
EELPWKKEKIDVVLESTGLFTSREKAELHLKAGAKKIIISAPATNEDITIVMGVNSNLYDPKKHNIISNASCTTNCLAPV
AKVLHETFGIEKGLVTTVHSYTNDQQILDLPHKDLRRARAAAMSMIPTSTGAAKAVALVLPELKGKLDGMAIRVPTPNVS
VVDLVVTLSKKADADKVNAALKKASKGSLKGVLGFSEEPLVSIDFNGNPLSSIVDAGCTKVIEGNMVKVLSWYDNETGFS
HRVVDLMKLIA
>Mature_330_residues
ALRVAINGFGRIGRSVLRAAIKEKGIEFVAINDLTDAKTLAHLLKYDSVHGPFPGKVEAGNGEIIVNGKPIKILAVRNPE
ELPWKKEKIDVVLESTGLFTSREKAELHLKAGAKKIIISAPATNEDITIVMGVNSNLYDPKKHNIISNASCTTNCLAPVA
KVLHETFGIEKGLVTTVHSYTNDQQILDLPHKDLRRARAAAMSMIPTSTGAAKAVALVLPELKGKLDGMAIRVPTPNVSV
VDLVVTLSKKADADKVNAALKKASKGSLKGVLGFSEEPLVSIDFNGNPLSSIVDAGCTKVIEGNMVKVLSWYDNETGFSH
RVVDLMKLIA

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7657116, Length=330, Percent_Identity=51.2121212121212, Blast_Score=341, Evalue=6e-94,
Organism=Homo sapiens, GI7669492, Length=331, Percent_Identity=51.963746223565, Blast_Score=336, Evalue=2e-92,
Organism=Escherichia coli, GI1788079, Length=332, Percent_Identity=53.9156626506024, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1789295, Length=330, Percent_Identity=47.2727272727273, Blast_Score=320, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI17534677, Length=334, Percent_Identity=50.8982035928144, Blast_Score=324, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI17534679, Length=334, Percent_Identity=50.8982035928144, Blast_Score=323, Evalue=8e-89,
Organism=Caenorhabditis elegans, GI32566163, Length=335, Percent_Identity=50.4477611940299, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI17568413, Length=335, Percent_Identity=50.4477611940299, Blast_Score=315, Evalue=2e-86,
Organism=Saccharomyces cerevisiae, GI6322409, Length=331, Percent_Identity=52.2658610271903, Blast_Score=337, Evalue=2e-93,
Organism=Saccharomyces cerevisiae, GI6322468, Length=331, Percent_Identity=51.3595166163142, Blast_Score=330, Evalue=2e-91,
Organism=Saccharomyces cerevisiae, GI6321631, Length=331, Percent_Identity=51.0574018126888, Blast_Score=328, Evalue=6e-91,
Organism=Drosophila melanogaster, GI85725000, Length=329, Percent_Identity=51.063829787234, Blast_Score=324, Evalue=5e-89,
Organism=Drosophila melanogaster, GI22023983, Length=329, Percent_Identity=51.063829787234, Blast_Score=324, Evalue=5e-89,
Organism=Drosophila melanogaster, GI17933600, Length=329, Percent_Identity=50.4559270516717, Blast_Score=321, Evalue=4e-88,
Organism=Drosophila melanogaster, GI18110149, Length=329, Percent_Identity=50.4559270516717, Blast_Score=321, Evalue=4e-88,
Organism=Drosophila melanogaster, GI19922412, Length=325, Percent_Identity=49.2307692307692, Blast_Score=310, Evalue=8e-85,

Paralogues:

None

Copy number: 220 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1840 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 740 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Min

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 35512; Mature: 35381

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALRVAINGFGRIGRSVLRAAIKEKGIEFVAINDLTDAKTLAHLLKYDSVHGPFPGKVEA
CEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEC
GNGEIIVNGKPIKILAVRNPEELPWKKEKIDVVLESTGLFTSREKAELHLKAGAKKIIIS
CCCEEEECCCCEEEEEECCCCCCCCCCHHEEEEEECCCCCCCCCCCEEEEECCCEEEEEE
APATNEDITIVMGVNSNLYDPKKHNIISNASCTTNCLAPVAKVLHETFGIEKGLVTTVHS
CCCCCCCEEEEEECCCCCCCCHHCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHEEHH
YTNDQQILDLPHKDLRRARAAAMSMIPTSTGAAKAVALVLPELKGKLDGMAIRVPTPNVS
CCCCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VVDLVVTLSKKADADKVNAALKKASKGSLKGVLGFSEEPLVSIDFNGNPLSSIVDAGCTK
CEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHCCHH
VIEGNMVKVLSWYDNETGFSHRVVDLMKLIA
HHCCCEEEEEEEECCCCCCHHHHHHHHHHHC
>Mature Secondary Structure 
ALRVAINGFGRIGRSVLRAAIKEKGIEFVAINDLTDAKTLAHLLKYDSVHGPFPGKVEA
EEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCCCCCCCEEEC
GNGEIIVNGKPIKILAVRNPEELPWKKEKIDVVLESTGLFTSREKAELHLKAGAKKIIIS
CCCEEEECCCCEEEEEECCCCCCCCCCHHEEEEEECCCCCCCCCCCEEEEECCCEEEEEE
APATNEDITIVMGVNSNLYDPKKHNIISNASCTTNCLAPVAKVLHETFGIEKGLVTTVHS
CCCCCCCEEEEEECCCCCCCCHHCCEECCCCCCHHHHHHHHHHHHHHHCCCCCHHHEEHH
YTNDQQILDLPHKDLRRARAAAMSMIPTSTGAAKAVALVLPELKGKLDGMAIRVPTPNVS
CCCCCCEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCC
VVDLVVTLSKKADADKVNAALKKASKGSLKGVLGFSEEPLVSIDFNGNPLSSIVDAGCTK
CEEEEEECCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCHHHHHHHCCHH
VIEGNMVKVLSWYDNETGFSHRVVDLMKLIA
HHCCCEEEEEEEECCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684782; 2227448; 2656407; 7408868; 193030; 3586018; 9175858 [H]