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The map label for this gene is pdxJ

Identifier: 78223092

GI number: 78223092

Start: 2099875

End: 2100594

Strand: Reverse

Name: pdxJ

Synonym: Gmet_1885

Alternate gene names: 78223092

Gene position: 2100594-2099875 (Counterclockwise)

Preceding gene: 78223093

Following gene: 78223091

Centisome position: 52.55

GC content: 58.75

Gene sequence:

>720_bases
GTGGCAAAGCTTGGCGTAAATATTGACCACGTGGCAACAATCAGGCAGGCGAGGGGAGGAGTGGAACCCGATCCCGTGGC
TGCCGCCGCTCTGGCGGAGTTGGCCGGAGCCGACGGGATTACCATTCACCTGCGGGAAGACCGCCGTCATATCCAGGACC
GGGACCTGAAACTCCTTCGCCAAACCGTGAAGACCAAGCTCAATCTGGAGATGGCGGCAACGGCAGAGATGGTTGCCATA
GCCCTCTCCGTAAAGCCCGACATGTGCACTCTCGTTCCCGAAAAGCGTCAGGAGCTCACTACCGAAGGTGGACTGGATGT
GCGAATTGCCATGCAGGGGATTGCTGAAGCGGTGGAAAGGCTCCAGAATGGCGGCATCGCGGTGAGCCTCTTTGTCGATC
CCGACCCCGACCAGGTGAAGGCTTCCAGCAAGGTTGGATCCGACTACATCGAGATACACACCGGGGCCTTTGCCGACGCA
AAGGATTGGAAGTCCGAGCAGGATGAGTTGGAGCGGATTGGGAACGCCATTAAGCTTGGCGCCAAGCTCGGTCTCGGTAT
CAATGCCGGTCATGGCCTTAATTACACCAACATCAGGAAGGTTGCGGCCCTGGGGGGCATCGAGGAGTATAACATCGGCC
ATTCCATCATCTCCCGGGCGGTCCTTGTGGGACTCGACCGGGCAGTGCGCGACATGGTGGATCTCATCAAGTACGCCTGA

Upstream 100 bases:

>100_bases
GCTGTTGCGGATCATGCTGGAAGGTCAGGACAAATACCAGATTACCGGGTGGGCCAAGGAGATTGCCGACCTGGTTGAGA
AGAAGATCGGAGGGAAGTAG

Downstream 100 bases:

>100_bases
GGTATCCGTGATTTTCGGCACCGGCATTGATATCGTCGACATAACCCGCTTCGAGCGTTTTCTGGAAGAGAGGAATACGC
GTCTGTTCGAGCGCCTGTTC

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MAKLGVNIDHVATIRQARGGVEPDPVAAAALAELAGADGITIHLREDRRHIQDRDLKLLRQTVKTKLNLEMAATAEMVAI
ALSVKPDMCTLVPEKRQELTTEGGLDVRIAMQGIAEAVERLQNGGIAVSLFVDPDPDQVKASSKVGSDYIEIHTGAFADA
KDWKSEQDELERIGNAIKLGAKLGLGINAGHGLNYTNIRKVAALGGIEEYNIGHSIISRAVLVGLDRAVRDMVDLIKYA

Sequences:

>Translated_239_residues
MAKLGVNIDHVATIRQARGGVEPDPVAAAALAELAGADGITIHLREDRRHIQDRDLKLLRQTVKTKLNLEMAATAEMVAI
ALSVKPDMCTLVPEKRQELTTEGGLDVRIAMQGIAEAVERLQNGGIAVSLFVDPDPDQVKASSKVGSDYIEIHTGAFADA
KDWKSEQDELERIGNAIKLGAKLGLGINAGHGLNYTNIRKVAALGGIEEYNIGHSIISRAVLVGLDRAVRDMVDLIKYA
>Mature_238_residues
AKLGVNIDHVATIRQARGGVEPDPVAAAALAELAGADGITIHLREDRRHIQDRDLKLLRQTVKTKLNLEMAATAEMVAIA
LSVKPDMCTLVPEKRQELTTEGGLDVRIAMQGIAEAVERLQNGGIAVSLFVDPDPDQVKASSKVGSDYIEIHTGAFADAK
DWKSEQDELERIGNAIKLGAKLGLGINAGHGLNYTNIRKVAALGGIEEYNIGHSIISRAVLVGLDRAVRDMVDLIKYA

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family

Homologues:

Organism=Escherichia coli, GI1788917, Length=233, Percent_Identity=58.3690987124464, Blast_Score=280, Evalue=6e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PDXJ_GEOMG (Q39UG0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384839.1
- ProteinModelPortal:   Q39UG0
- SMR:   Q39UG0
- STRING:   Q39UG0
- GeneID:   3739161
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1885
- NMPDR:   fig|269799.3.peg.2148
- eggNOG:   COG0854
- HOGENOM:   HBG299314
- OMA:   AGHGLNY
- PhylomeDB:   Q39UG0
- ProtClustDB:   PRK05265
- BioCyc:   GMET269799:GMET_1885-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00279
- InterPro:   IPR013785
- InterPro:   IPR004569
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00559

Pfam domain/function: PF03740 PdxJ; SSF63892 PyrdxlP_synth_PdxJ

EC number: =2.6.99.2

Molecular weight: Translated: 25689; Mature: 25558

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: ACT_SITE 43-43 ACT_SITE 70-70 ACT_SITE 191-191 BINDING 7-7 BINDING 18-18 BINDING 45-45 BINDING 50-50 BINDING 100-100 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLGVNIDHVATIRQARGGVEPDPVAAAALAELAGADGITIHLREDRRHIQDRDLKLLR
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHH
QTVKTKLNLEMAATAEMVAIALSVKPDMCTLVPEKRQELTTEGGLDVRIAMQGIAEAVER
HHHHHHHCHHHHHHHHHHHEEEECCCCCEECCCHHHHHHHCCCCCEEEHHHHHHHHHHHH
LQNGGIAVSLFVDPDPDQVKASSKVGSDYIEIHTGAFADAKDWKSEQDELERIGNAIKLG
HHCCCEEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHH
AKLGLGINAGHGLNYTNIRKVAALGGIEEYNIGHSIISRAVLVGLDRAVRDMVDLIKYA
HHHCCCCCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AKLGVNIDHVATIRQARGGVEPDPVAAAALAELAGADGITIHLREDRRHIQDRDLKLLR
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHH
QTVKTKLNLEMAATAEMVAIALSVKPDMCTLVPEKRQELTTEGGLDVRIAMQGIAEAVER
HHHHHHHCHHHHHHHHHHHEEEECCCCCEECCCHHHHHHHCCCCCEEEHHHHHHHHHHHH
LQNGGIAVSLFVDPDPDQVKASSKVGSDYIEIHTGAFADAKDWKSEQDELERIGNAIKLG
HHCCCEEEEEEECCCHHHHHHHHHCCCCEEEEECCCCCCCHHHCCHHHHHHHHHHHHHHH
AKLGLGINAGHGLNYTNIRKVAALGGIEEYNIGHSIISRAVLVGLDRAVRDMVDLIKYA
HHHCCCCCCCCCCCHHHHHHHHHHCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA