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The map label for this gene is yjeA [H]

Identifier: 78223046

GI number: 78223046

Start: 2052325

End: 2053248

Strand: Direct

Name: yjeA [H]

Synonym: Gmet_1839

Alternate gene names: 78223046

Gene position: 2052325-2053248 (Clockwise)

Preceding gene: 78223045

Following gene: 78223051

Centisome position: 51.34

GC content: 59.31

Gene sequence:

>924_bases
ATGACGGCAAACTGGCGCCTTGCCCGCCGCCATGGAGCGCTCCGGGCACGGGCCCGCATCATACAGGAAATAAGACGCTT
TTTTATTGAAGGGGGGTATCTCGAAGTGGAGACCCCCCTTCGCATTCCTGCTTGTGCGCCCGAGTCCCACATTGACCCGG
TGCCGGCGGACGGCTGGTTCCTCCAGACCTCTCCCGAAATCTGTATGAAACGGATGTTGGCGGCGGGTTACGAGCGGTTA
TTCCAGATCTCCCACTGCTGGCGCGCCGGTGAGCGGGGAAGAATGCATGTCCCGGAGTTCACCATGCTTGAGTGGTATCG
GGCAGGTACAGACTATCGGGGGGTGATGGATGAAACCGAGTTGCTGATAAGGTTCGTGGCTGAAGGGGCGGGATTCGGCG
GGCCACTTTCGTTCCGTGGCAAGGAGATCGACATTACCGCTGTGTGGGAGCGGATCACCGTGCGTGACGCGTTTGTCAGA
TATGGTGGAATAACCATGGATGAGGCCCTTGAAAGGGACATCTTCGACGAGATGATGGTCGAACAAATCGAACCGCGTCT
CGGCATCGGCCGTCCCACGTTCATCCATGACTACCCTTCCTGCCGCAGCGCCCTGGCGCGGCTAAAGAAAGACGATCCAG
CCGTTGCAGAGCGATTCGAGCTTTATCTTGGTGGTGTAGAGATTGCCAATGCCTTTTCTGAGCTGACCGACCCAGTGGAG
CAGCGCGCCCGCTTTGAAAGCGAAAACGCCTTCCGTACCTCCCGGGGGCAGACGTCCACTCCCCTCCCCGAGAAGTTCCT
TGCCGAACTGACTGCCATGCCCCAGGCGGCCGGAATCGCCCTCGGCATCGACCGGCTCGTGATGGTGTTGCTTGATGCCG
ACACCATTGACGACGTGGTGGCGTTTCCCCCTGAGGAACTGTAA

Upstream 100 bases:

>100_bases
GGCTACGTCCTGCGCGTACCCCCCTTCATAGAGGAAGGCGAGATGATTGTGGTCGACACCCGCACCGGTGATTACTCCAC
GAGGGTCAAGGGGTAAGCCG

Downstream 100 bases:

>100_bases
CTCCCCTCCCCCTCACCGATCCTGGTATTCGACAATCTCTCCCCGGTAGTTCCGTAACAGCCACGTATCACCCCGCCGCT
CCACGGCATCGGGGAGAAGC

Product: tRNA synthetase, class II (D, K and N)

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTANWRLARRHGALRARARIIQEIRRFFIEGGYLEVETPLRIPACAPESHIDPVPADGWFLQTSPEICMKRMLAAGYERL
FQISHCWRAGERGRMHVPEFTMLEWYRAGTDYRGVMDETELLIRFVAEGAGFGGPLSFRGKEIDITAVWERITVRDAFVR
YGGITMDEALERDIFDEMMVEQIEPRLGIGRPTFIHDYPSCRSALARLKKDDPAVAERFELYLGGVEIANAFSELTDPVE
QRARFESENAFRTSRGQTSTPLPEKFLAELTAMPQAAGIALGIDRLVMVLLDADTIDDVVAFPPEEL

Sequences:

>Translated_307_residues
MTANWRLARRHGALRARARIIQEIRRFFIEGGYLEVETPLRIPACAPESHIDPVPADGWFLQTSPEICMKRMLAAGYERL
FQISHCWRAGERGRMHVPEFTMLEWYRAGTDYRGVMDETELLIRFVAEGAGFGGPLSFRGKEIDITAVWERITVRDAFVR
YGGITMDEALERDIFDEMMVEQIEPRLGIGRPTFIHDYPSCRSALARLKKDDPAVAERFELYLGGVEIANAFSELTDPVE
QRARFESENAFRTSRGQTSTPLPEKFLAELTAMPQAAGIALGIDRLVMVLLDADTIDDVVAFPPEEL
>Mature_306_residues
TANWRLARRHGALRARARIIQEIRRFFIEGGYLEVETPLRIPACAPESHIDPVPADGWFLQTSPEICMKRMLAAGYERLF
QISHCWRAGERGRMHVPEFTMLEWYRAGTDYRGVMDETELLIRFVAEGAGFGGPLSFRGKEIDITAVWERITVRDAFVRY
GGITMDEALERDIFDEMMVEQIEPRLGIGRPTFIHDYPSCRSALARLKKDDPAVAERFELYLGGVEIANAFSELTDPVEQ
RARFESENAFRTSRGQTSTPLPEKFLAELTAMPQAAGIALGIDRLVMVLLDADTIDDVVAFPPEEL

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=325, Percent_Identity=28.6153846153846, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI5031815, Length=325, Percent_Identity=28.6153846153846, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI87082379, Length=319, Percent_Identity=37.6175548589342, Blast_Score=196, Evalue=1e-51,
Organism=Escherichia coli, GI1789256, Length=321, Percent_Identity=32.0872274143302, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1790571, Length=316, Percent_Identity=31.9620253164557, Blast_Score=158, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI71994340, Length=327, Percent_Identity=30.5810397553517, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17535925, Length=327, Percent_Identity=30.5810397553517, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17535927, Length=327, Percent_Identity=30.5810397553517, Blast_Score=132, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320242, Length=328, Percent_Identity=29.8780487804878, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24640851, Length=333, Percent_Identity=29.7297297297297, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI24640849, Length=333, Percent_Identity=29.7297297297297, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 34815; Mature: 34684

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANWRLARRHGALRARARIIQEIRRFFIEGGYLEVETPLRIPACAPESHIDPVPADGWF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEE
LQTSPEICMKRMLAAGYERLFQISHCWRAGERGRMHVPEFTMLEWYRAGTDYRGVMDETE
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHH
LLIRFVAEGAGFGGPLSFRGKEIDITAVWERITVRDAFVRYGGITMDEALERDIFDEMMV
HHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EQIEPRLGIGRPTFIHDYPSCRSALARLKKDDPAVAERFELYLGGVEIANAFSELTDPVE
HHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
QRARFESENAFRTSRGQTSTPLPEKFLAELTAMPQAAGIALGIDRLVMVLLDADTIDDVV
HHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHH
AFPPEEL
CCCCCCC
>Mature Secondary Structure 
TANWRLARRHGALRARARIIQEIRRFFIEGGYLEVETPLRIPACAPESHIDPVPADGWF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEE
LQTSPEICMKRMLAAGYERLFQISHCWRAGERGRMHVPEFTMLEWYRAGTDYRGVMDETE
EECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCCCHHHHH
LLIRFVAEGAGFGGPLSFRGKEIDITAVWERITVRDAFVRYGGITMDEALERDIFDEMMV
HHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
EQIEPRLGIGRPTFIHDYPSCRSALARLKKDDPAVAERFELYLGGVEIANAFSELTDPVE
HHHCCCCCCCCCCEECCCHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHH
QRARFESENAFRTSRGQTSTPLPEKFLAELTAMPQAAGIALGIDRLVMVLLDADTIDDVV
HHHHCCCCCCHHCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHH
AFPPEEL
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA