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The map label for this gene is pyrB

Identifier: 78222978

GI number: 78222978

Start: 1983928

End: 1984863

Strand: Direct

Name: pyrB

Synonym: Gmet_1769

Alternate gene names: 78222978

Gene position: 1983928-1984863 (Clockwise)

Preceding gene: 78222977

Following gene: 304570571

Centisome position: 49.63

GC content: 59.51

Gene sequence:

>936_bases
ATGGGATTCAAGCACAAGGACATCATAGCGCTGAAAGACCTCAGCAAGGATGAGATTGAACTGCTGATCTCGACCGCCGA
AAACATGCGGGAGATCAACAGCCGCGATATCAAGAAGGTGCCGACCCTGCGCGGCAAGACCATCATCAATCTGTTCTACG
AGTCATCGACCCGTACCCGTACCTCCTTCGAGATCGCGGGAAAGCGGCTGTCGGCCGACACCGTCAACATTACCGCATCC
ACGAGTTCGGCAACCAAGGGGGAGACGCTCCTCGATACCGCAAAAAACCTGGAAGCGATGAAACCGGACATCGTCGTCAT
GCGCCACGCCGTTTCCGGCTCCCACTACTTCCTGGCCAAACGGCTCTCCTGCTCCATCATCAACGCCGGTGACGGCGCCC
ATGAGCACCCCTCCCAGGGGCTTCTCGACATGCTGACCATCAAGGACCGCTACGGCCGGCTTGACGGACTCAAGGTGGCC
ATTGTCGGCGATATCACCCATAGCCGGGTGGCACGCTCCGATATCGCAGGTTTCACCATGATGGGATCCCGGGTGTTTCT
GGCGGGGCCTCCCACCATGATGCCTCCGGGGGTGGAGCGGCTCGGCAACGTCACTGTCTGCGCCACCATGAAGGAAGCGA
TCCAGGATGCCGACGTAATCATAATGCTCCGCATCCAGCAGGAGCGGCAGGGGAAAACCCTGATACCCAATACCCGTGAG
TATTCCCGCTATTACGGCCTTAACCCCGAAAACCTCAAGTGGGCGAAGCCCGACGCCATGGTCATGCACCCCGGCCCCAT
CAACCGCGGTGTGGAACTCTCTTCCTACGTGGCGGACGGCGCCAATTCCCACGTTCTCAAACAGGTGGAAAACGGCGTGG
CGGTGCGGATGGCGATGCTCTATCACGTGAGCGGGGGAACGCTGGTTACGGAGTGA

Upstream 100 bases:

>100_bases
AAGTGGTTCTGGAAAAGTGATTTGCGTCGGGGGCAACCCAGGCGGTTACTTTTGACAGGGCAGGCACGGGGCCTAGCCCT
ACGCGAGATGGGGGGAAGCG

Downstream 100 bases:

>100_bases
TTCAATGCTTGTAGGGGCGAATCTCGCATTCGCCCGATTTGGGCGATCACAAGGATCGTCCCTACACCAGGACGCGAGGT
GATCCATGAATTTGTTGATA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MGFKHKDIIALKDLSKDEIELLISTAENMREINSRDIKKVPTLRGKTIINLFYESSTRTRTSFEIAGKRLSADTVNITAS
TSSATKGETLLDTAKNLEAMKPDIVVMRHAVSGSHYFLAKRLSCSIINAGDGAHEHPSQGLLDMLTIKDRYGRLDGLKVA
IVGDITHSRVARSDIAGFTMMGSRVFLAGPPTMMPPGVERLGNVTVCATMKEAIQDADVIIMLRIQQERQGKTLIPNTRE
YSRYYGLNPENLKWAKPDAMVMHPGPINRGVELSSYVADGANSHVLKQVENGVAVRMAMLYHVSGGTLVTE

Sequences:

>Translated_311_residues
MGFKHKDIIALKDLSKDEIELLISTAENMREINSRDIKKVPTLRGKTIINLFYESSTRTRTSFEIAGKRLSADTVNITAS
TSSATKGETLLDTAKNLEAMKPDIVVMRHAVSGSHYFLAKRLSCSIINAGDGAHEHPSQGLLDMLTIKDRYGRLDGLKVA
IVGDITHSRVARSDIAGFTMMGSRVFLAGPPTMMPPGVERLGNVTVCATMKEAIQDADVIIMLRIQQERQGKTLIPNTRE
YSRYYGLNPENLKWAKPDAMVMHPGPINRGVELSSYVADGANSHVLKQVENGVAVRMAMLYHVSGGTLVTE
>Mature_310_residues
GFKHKDIIALKDLSKDEIELLISTAENMREINSRDIKKVPTLRGKTIINLFYESSTRTRTSFEIAGKRLSADTVNITAST
SSATKGETLLDTAKNLEAMKPDIVVMRHAVSGSHYFLAKRLSCSIINAGDGAHEHPSQGLLDMLTIKDRYGRLDGLKVAI
VGDITHSRVARSDIAGFTMMGSRVFLAGPPTMMPPGVERLGNVTVCATMKEAIQDADVIIMLRIQQERQGKTLIPNTREY
SRYYGLNPENLKWAKPDAMVMHPGPINRGVELSSYVADGANSHVLKQVENGVAVRMAMLYHVSGGTLVTE

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=309, Percent_Identity=34.6278317152104, Blast_Score=174, Evalue=6e-44,
Organism=Homo sapiens, GI38788445, Length=169, Percent_Identity=31.3609467455621, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI2367364, Length=316, Percent_Identity=33.2278481012658, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1790703, Length=321, Percent_Identity=23.98753894081, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=322, Percent_Identity=31.3664596273292, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322331, Length=313, Percent_Identity=31.9488817891374, Blast_Score=147, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=32.484076433121, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_GEOMG (Q39US4)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_384725.1
- HSSP:   P0A786
- ProteinModelPortal:   Q39US4
- SMR:   Q39US4
- STRING:   Q39US4
- GeneID:   3741445
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_1769
- NMPDR:   fig|269799.3.peg.2086
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- PhylomeDB:   Q39US4
- ProtClustDB:   PRK00856
- BioCyc:   GMET269799:GMET_1769-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 34142; Mature: 34011

Theoretical pI: Translated: 9.46; Mature: 9.46

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFKHKDIIALKDLSKDEIELLISTAENMREINSRDIKKVPTLRGKTIINLFYESSTRTR
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEECCCCCCE
TSFEIAGKRLSADTVNITASTSSATKGETLLDTAKNLEAMKPDIVVMRHAVSGSHYFLAK
EEHEECCCEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEE
RLSCSIINAGDGAHEHPSQGLLDMLTIKDRYGRLDGLKVAIVGDITHSRVARSDIAGFTM
ECEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHCCEEE
MGSRVFLAGPPTMMPPGVERLGNVTVCATMKEAIQDADVIIMLRIQQERQGKTLIPNTRE
ECCEEEEECCCCCCCCCHHHHCCEEEEEEHHHHHCCCCEEEEEEEECCCCCCCCCCCCHH
YSRYYGLNPENLKWAKPDAMVMHPGPINRGVELSSYVADGANSHVLKQVENGVAVRMAML
HHHHCCCCCCCCEECCCCEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHCCHHEEEEEE
YHVSGGTLVTE
EEECCCEEECC
>Mature Secondary Structure 
GFKHKDIIALKDLSKDEIELLISTAENMREINSRDIKKVPTLRGKTIINLFYESSTRTR
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEEECCCCCCE
TSFEIAGKRLSADTVNITASTSSATKGETLLDTAKNLEAMKPDIVVMRHAVSGSHYFLAK
EEHEECCCEECCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEE
RLSCSIINAGDGAHEHPSQGLLDMLTIKDRYGRLDGLKVAIVGDITHSRVARSDIAGFTM
ECEEEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHCCEEE
MGSRVFLAGPPTMMPPGVERLGNVTVCATMKEAIQDADVIIMLRIQQERQGKTLIPNTRE
ECCEEEEECCCCCCCCCHHHHCCEEEEEEHHHHHCCCCEEEEEEEECCCCCCCCCCCCHH
YSRYYGLNPENLKWAKPDAMVMHPGPINRGVELSSYVADGANSHVLKQVENGVAVRMAML
HHHHCCCCCCCCEECCCCEEEECCCCCCCCCCHHHHHHCCCCHHHHHHHHCCHHEEEEEE
YHVSGGTLVTE
EEECCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA