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The map label for this gene is 78222899

Identifier: 78222899

GI number: 78222899

Start: 1896894

End: 1898180

Strand: Direct

Name: 78222899

Synonym: Gmet_1690

Alternate gene names: NA

Gene position: 1896894-1898180 (Clockwise)

Preceding gene: 78222898

Following gene: 78222900

Centisome position: 47.45

GC content: 64.26

Gene sequence:

>1287_bases
ATGAAGCTCCTCTTCGTCTCTCCCGGATGGCCGCGGGGGAGGCTCTGGGGGGAGCTGGGCTTCAAGTTTCCTTCCCTTTC
CCTTGCCGCGCTGGCGGCCGTCACCCCCGAATCGTGGGACGTGGCCCTTTGCGACGAGAATATCGAGCCGCTCGATCTGA
ATTCCGACGCCGATCTCGTGGCAATTACCGCCATGACTCCCCAGGCACCCCAGGCCTACCGGATCGCTGCCGCGTTCCGC
GCCCGGGGGAAGCGGGTGGTCATGGGGGGCTTCCACGCCAGCAACCTGCCGGAAGAGGCGCTCCAGCACGTGGATGCCGT
GGTGGTGGGAGAGGGTGAGGTGGTCTGGCCCCAACTCCTGGCCGACCTTGAGCGGGGCGAGCTGCAGCGCATCTACCGGG
CCGCAACCGCACTCGCCATGGAAGCGATTCCCGTGGCCCGTCGTGAGCTATTCCAGGGGAAGCGGTACCTCTTCACCAAT
ACCCTCCAGACCACCCGGGGCTGTCCCTTTGATTGTGAATTCTGCTCCGTTACCGCCTTTTACGGCCGTAGGTACCGCAA
GCGGCCGGTGGATAACGTTCTGGCCGAGCTGGAACTCCTCCGCCGCCGGAACTCCTTCGCCTTTTTCGTGGATGACAACC
TGGTGGCCGACCGCACCTACGCCCTCTCCCTCTTTGCCGGCATGAAGGGGATGGGGTTCAAGTGGCTTTCCCACGCCCCC
ATCGATTTCGCCCACGACCGGGAGTTCCTGCGGGCCGCCGGCGAGGCCGGCTGCGTCGGGATGTTCGTCGGCTTCGAGTC
CCTGAACCAGGACGCCCTTGCCGCCATGGGGAAGGTGACCAACACGGCCCGTTCCTACCTGGAGGATGCCCAAGCCTTCC
GTGACAACGGCATCGGCATTCTCGGCTCGTTCGTCCTCGGCTGCGACAACGACACTCCCGCCGTCTTCGAGGGGCTCCTC
CGCTTCTGCGAGGAGGCGCGGCTAGAGGCGGCGATTTTCCCGATTCTTACCCCCTATCCCGGCACCGAGGTCCGACGGCG
GCTTGAGGCGGAAGGCCGGATCATCTCCAGCGACTGGCAGGACTACGACATGGAGCACGTCACTTTCCTCCCGCGTGGGA
TGTCCGTTGACGAGCTTCAGCAGGGGTATGACTGGCTGAACCGTTCCTTTTACTCCTTACCATCCATGTACCGGAGAATC
TTCAAGGCCCACCGCTCGGTCCAGGTATTCGCCCCCATGAATTTCGGATTCCGCAGCGCTATCCGCCGGAAGGGGAGGGT
CGCGTGA

Upstream 100 bases:

>100_bases
TGGAGAAGCAGTACGGCGTCGTTGTTCCCGATGCTGCCACCGGGACCAAGGTCTTTCAGTCGGTCCGTACCGTGGCCGAC
TTTGTCGCGGAAAACCGTAA

Downstream 100 bases:

>100_bases
TCTGCTTCATGTTTCCCGGCCAACCCCTGACCTTTGAAACAACGGTTCCCGACGATCCCGATTTCCGGCAGGCAACGGCA
CTGACCCTGGACCGGACTGG

Product: radical SAM family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MKLLFVSPGWPRGRLWGELGFKFPSLSLAALAAVTPESWDVALCDENIEPLDLNSDADLVAITAMTPQAPQAYRIAAAFR
ARGKRVVMGGFHASNLPEEALQHVDAVVVGEGEVVWPQLLADLERGELQRIYRAATALAMEAIPVARRELFQGKRYLFTN
TLQTTRGCPFDCEFCSVTAFYGRRYRKRPVDNVLAELELLRRRNSFAFFVDDNLVADRTYALSLFAGMKGMGFKWLSHAP
IDFAHDREFLRAAGEAGCVGMFVGFESLNQDALAAMGKVTNTARSYLEDAQAFRDNGIGILGSFVLGCDNDTPAVFEGLL
RFCEEARLEAAIFPILTPYPGTEVRRRLEAEGRIISSDWQDYDMEHVTFLPRGMSVDELQQGYDWLNRSFYSLPSMYRRI
FKAHRSVQVFAPMNFGFRSAIRRKGRVA

Sequences:

>Translated_428_residues
MKLLFVSPGWPRGRLWGELGFKFPSLSLAALAAVTPESWDVALCDENIEPLDLNSDADLVAITAMTPQAPQAYRIAAAFR
ARGKRVVMGGFHASNLPEEALQHVDAVVVGEGEVVWPQLLADLERGELQRIYRAATALAMEAIPVARRELFQGKRYLFTN
TLQTTRGCPFDCEFCSVTAFYGRRYRKRPVDNVLAELELLRRRNSFAFFVDDNLVADRTYALSLFAGMKGMGFKWLSHAP
IDFAHDREFLRAAGEAGCVGMFVGFESLNQDALAAMGKVTNTARSYLEDAQAFRDNGIGILGSFVLGCDNDTPAVFEGLL
RFCEEARLEAAIFPILTPYPGTEVRRRLEAEGRIISSDWQDYDMEHVTFLPRGMSVDELQQGYDWLNRSFYSLPSMYRRI
FKAHRSVQVFAPMNFGFRSAIRRKGRVA
>Mature_428_residues
MKLLFVSPGWPRGRLWGELGFKFPSLSLAALAAVTPESWDVALCDENIEPLDLNSDADLVAITAMTPQAPQAYRIAAAFR
ARGKRVVMGGFHASNLPEEALQHVDAVVVGEGEVVWPQLLADLERGELQRIYRAATALAMEAIPVARRELFQGKRYLFTN
TLQTTRGCPFDCEFCSVTAFYGRRYRKRPVDNVLAELELLRRRNSFAFFVDDNLVADRTYALSLFAGMKGMGFKWLSHAP
IDFAHDREFLRAAGEAGCVGMFVGFESLNQDALAAMGKVTNTARSYLEDAQAFRDNGIGILGSFVLGCDNDTPAVFEGLL
RFCEEARLEAAIFPILTPYPGTEVRRRLEAEGRIISSDWQDYDMEHVTFLPRGMSVDELQQGYDWLNRSFYSLPSMYRRI
FKAHRSVQVFAPMNFGFRSAIRRKGRVA

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 48077; Mature: 48077

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLFVSPGWPRGRLWGELGFKFPSLSLAALAAVTPESWDVALCDENIEPLDLNSDADLV
CEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEE
AITAMTPQAPQAYRIAAAFRARGKRVVMGGFHASNLPEEALQHVDAVVVGEGEVVWPQLL
EEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCEEEECCCCEEHHHHH
ADLERGELQRIYRAATALAMEAIPVARRELFQGKRYLFTNTLQTTRGCPFDCEFCSVTAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCCHHHHHHHH
YGRRYRKRPVDNVLAELELLRRRNSFAFFVDDNLVADRTYALSLFAGMKGMGFKWLSHAP
HHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCEECCHHHHHHHHHCCCCCCCHHHCCCC
IDFAHDREFLRAAGEAGCVGMFVGFESLNQDALAAMGKVTNTARSYLEDAQAFRDNGIGI
CCHHHHHHHHHHCCCCCEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LGSFVLGCDNDTPAVFEGLLRFCEEARLEAAIFPILTPYPGTEVRRRLEAEGRIISSDWQ
EEHEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEECCCC
DYDMEHVTFLPRGMSVDELQQGYDWLNRSFYSLPSMYRRIFKAHRSVQVFAPMNFGFRSA
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
IRRKGRVA
HHHCCCCC
>Mature Secondary Structure
MKLLFVSPGWPRGRLWGELGFKFPSLSLAALAAVTPESWDVALCDENIEPLDLNSDADLV
CEEEEECCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCEE
AITAMTPQAPQAYRIAAAFRARGKRVVMGGFHASNLPEEALQHVDAVVVGEGEVVWPQLL
EEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCEEEECCCCEEHHHHH
ADLERGELQRIYRAATALAMEAIPVARRELFQGKRYLFTNTLQTTRGCPFDCEFCSVTAF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHCCCCCCCCHHHHHHHH
YGRRYRKRPVDNVLAELELLRRRNSFAFFVDDNLVADRTYALSLFAGMKGMGFKWLSHAP
HHHHHHCCCHHHHHHHHHHHHHCCCEEEEEECCEECCHHHHHHHHHCCCCCCCHHHCCCC
IDFAHDREFLRAAGEAGCVGMFVGFESLNQDALAAMGKVTNTARSYLEDAQAFRDNGIGI
CCHHHHHHHHHHCCCCCEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LGSFVLGCDNDTPAVFEGLLRFCEEARLEAAIFPILTPYPGTEVRRRLEAEGRIISSDWQ
EEHEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEECCCCCHHHHHHHHHCCCEEECCCC
DYDMEHVTFLPRGMSVDELQQGYDWLNRSFYSLPSMYRRIFKAHRSVQVFAPMNFGFRSA
CCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHH
IRRKGRVA
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]