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The map label for this gene is prfB [H]

Identifier: 78222830

GI number: 78222830

Start: 1820172

End: 1820495

Strand: Direct

Name: prfB [H]

Synonym: Gmet_1621

Alternate gene names: 78222830

Gene position: 1820172-1820495 (Clockwise)

Preceding gene: 78222827

Following gene: 78222831

Centisome position: 45.53

GC content: 58.95

Gene sequence:

>324_bases
ATGATCGAGATCAATGAGGCAGATCTTAAATTCGAGTTCTATCGCGGTTCGGGACCAGGGGGCCAGCACCGCAATGTGAC
TGAAAGCGGCGTCCGCATCAGGCATGTTCCAACCGGTGTCGTGGTCCAGGCCTGCGAAAGCCGTTCCCAGGCCCGCAACC
GCGAGATTGCCCTGGAGCGACTGCGCCAGATACTGGAGCGCCGTGAACGGCGTGTAAAAAAACGGGTTGCCACCACGGTT
CCCCGCGGCCAGAAAGAGAAGCGGATCGAAGAGAAGAAACGGCGCTCCCTGACCAAGCGCCTGAGAACTCCTTCAGAGGA
TTAG

Upstream 100 bases:

>100_bases
CCGCTTGGTAATGGATTTATTATTGATGTTCGTCCTGTTGTCTGTCAACCTTGGACATTTACGAGTCTGATTCCGATTCA
GTTGGGCCGGGGGAACCGCC

Downstream 100 bases:

>100_bases
CCGTATGCGTATTTGGCTAATTCCTTGACCTTGCGGCGTTTCTCGGGTATTCTTGCCCACCACTTCACCCCACGGAGCCC
CCATGACCCGACCCACCTGG

Product: class I peptide chain release factor

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 107; Mature: 107

Protein sequence:

>107_residues
MIEINEADLKFEFYRGSGPGGQHRNVTESGVRIRHVPTGVVVQACESRSQARNREIALERLRQILERRERRVKKRVATTV
PRGQKEKRIEEKKRRSLTKRLRTPSED

Sequences:

>Translated_107_residues
MIEINEADLKFEFYRGSGPGGQHRNVTESGVRIRHVPTGVVVQACESRSQARNREIALERLRQILERRERRVKKRVATTV
PRGQKEKRIEEKKRRSLTKRLRTPSED
>Mature_107_residues
MIEINEADLKFEFYRGSGPGGQHRNVTESGVRIRHVPTGVVVQACESRSQARNREIALERLRQILERRERRVKKRVATTV
PRGQKEKRIEEKKRRSLTKRLRTPSED

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=59, Percent_Identity=52.5423728813559, Blast_Score=68, Evalue=1e-12,
Organism=Homo sapiens, GI34577120, Length=75, Percent_Identity=42.6666666666667, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI2367172, Length=73, Percent_Identity=49.3150684931507, Blast_Score=75, Evalue=7e-16,
Organism=Escherichia coli, GI1787462, Length=59, Percent_Identity=50.8474576271186, Blast_Score=69, Evalue=7e-14,
Organism=Drosophila melanogaster, GI19921226, Length=61, Percent_Identity=57.3770491803279, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 12548; Mature: 12548

Theoretical pI: Translated: 11.72; Mature: 11.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEINEADLKFEFYRGSGPGGQHRNVTESGVRIRHVPTGVVVQACESRSQARNREIALER
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
LRQILERRERRVKKRVATTVPRGQKEKRIEEKKRRSLTKRLRTPSED
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MIEINEADLKFEFYRGSGPGGQHRNVTESGVRIRHVPTGVVVQACESRSQARNREIALER
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHH
LRQILERRERRVKKRVATTVPRGQKEKRIEEKKRRSLTKRLRTPSED
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA