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The map label for this gene is 78222446

Identifier: 78222446

GI number: 78222446

Start: 1388516

End: 1390093

Strand: Reverse

Name: 78222446

Synonym: Gmet_1232

Alternate gene names: NA

Gene position: 1390093-1388516 (Counterclockwise)

Preceding gene: 78222457

Following gene: 78222445

Centisome position: 34.77

GC content: 59.25

Gene sequence:

>1578_bases
ATGGAAAAGCGATTCATACGGTCACTCCTGAAGCCTTCAGCCTACCCGGAACACACGGACTCCGTGGAGCTCGTCCAGAC
CCATGTCTCCTACATCTTTCTGACTGACCGCTTCGCCTACAAGGTCAAAAAGCCGGTGGATTTCGGCTTCCTCAATTTTT
CAACCATAGATCGACGCCGCTTTTACTGCAACGAAGAAGTTCGCCTCAATCGACGCCTCTGCCCTGACATCTACCTCGGC
GTGGAGGAAGTGAGGGAAACGCCTGGCGGGGCCGGATTTTCCGGCGATGGACGGGTCATCGATTACGCGGTCAAGATGAA
GCGGCTCCCGGCGCAACGGATGCTCGACCGACTGCTGAGTGCGGGTGAGGTTTCCGTGGCTGATATCCGAGCCATTGCCC
GGGTAATCGGCGAATTTCACCGCCGCGCCGGACGGAGCAGGGAGATCGACAGCTACGGCGGAGCCGACGTCATTCGAGCC
AACTGGGAAGAAAATTTCCGTCAGACCCGCCAGTTTGCGGGAGAAACCCTGAGCGGCCGGGCTCTTCGCTTCATCGAAGA
ATGGGTCGAACGCTTCATGAAAGAAAATGCCGTCATCTTCGCCCAACGCGTTGCCGGCGGCTTCATCCGGGACTGCGACG
GCGACATCCACCTGGAAAACATCTGCCTGGATTCCGACGGCAGTATCTGCATCTTTGACTGCATCGAATTCAATACCCGT
TTCCGCTACAGCGACACGGCCGCCGACATCGCCTTCCTCCTGATGGATTTCGACGTTAACCGCAGATCTGACCTCTGCTC
GCCGTTCCTGGATGAATACATACGGGCAACCGGCGATGACGGAGTCACCGACGTCCTCGATTTCTACAAGGTCTATCGGG
CCTGCGTCAGGGGAAAGGTGGAGAGCATGCGGCTTCTCGACCCGCAGATTCCGCCGGCGGACAAGGCCGCAGCCAAGGAA
CGCTCCCGGCGCTATTTCCGTCTTGCACGCGGCTACATCGCCCGCCGGGCTTTCCCGCCGTCGCTCATCCTGACCTGTGG
CCTCACGGGAACCGGCAAGAGCTACACGGCGCGGGAACTGGCCTTCGACCTTGGGCTGGAAATAGTCAGTTCCGATGTGG
TAAGAAAGGAGCTGACAGGTTCAGCACCGGAAGAGCACCATTGGGACCGCTACAGAGAAGGCATCTACACGGAGTCTGTC
ACCGCCCGCACCTACGGAGAGCTCTTCAAGCGGGCCGAGACGGCCCTGAATGCGGGACGCAGCATCATCGTGGACGCCAC
CTTCCGCCGCAGAACCGATCGTGACCTCTTTCGTCAACTGGCCGATCGTCACGGTTCCCGTTTCCATCTGCTCCACGCCG
TCTGCCCGGAAGAAGCCGTCAGGCAGCGTCTGGAGGCCAGGAGAAACCAGTGTGATGAACCTTCGGACGGCCGGTGGGAT
ATCTACCTTTTACAGAAAGAGGAGTTCGAAGCCCCTGCCGACGACGAATCAGGCACGATCAGGATCGACACCTCCAAACC
GGTCACCGAGCTGCTCGAAACGCTCCTTGACGCCATGGGGATACTGCCGTGTGGATGA

Upstream 100 bases:

>100_bases
ATAACCCTGACCCGTCAAATGAATATAAGGAAGCGCAAACCTTGCGCGGAGCGGCGTTTATGGTACTGTGTATGGTAACT
TTCCGCCCTGTGGGGACGGC

Downstream 100 bases:

>100_bases
AATTGCGGGTTGCCCTCTTCAAACGGTTGAGACTGAGCGAATCGTGGGTGATTTTCTTCATCCTGGGCTTCGTCATGATG
AACTATCCGTTCATCCAGAT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MEKRFIRSLLKPSAYPEHTDSVELVQTHVSYIFLTDRFAYKVKKPVDFGFLNFSTIDRRRFYCNEEVRLNRRLCPDIYLG
VEEVRETPGGAGFSGDGRVIDYAVKMKRLPAQRMLDRLLSAGEVSVADIRAIARVIGEFHRRAGRSREIDSYGGADVIRA
NWEENFRQTRQFAGETLSGRALRFIEEWVERFMKENAVIFAQRVAGGFIRDCDGDIHLENICLDSDGSICIFDCIEFNTR
FRYSDTAADIAFLLMDFDVNRRSDLCSPFLDEYIRATGDDGVTDVLDFYKVYRACVRGKVESMRLLDPQIPPADKAAAKE
RSRRYFRLARGYIARRAFPPSLILTCGLTGTGKSYTARELAFDLGLEIVSSDVVRKELTGSAPEEHHWDRYREGIYTESV
TARTYGELFKRAETALNAGRSIIVDATFRRRTDRDLFRQLADRHGSRFHLLHAVCPEEAVRQRLEARRNQCDEPSDGRWD
IYLLQKEEFEAPADDESGTIRIDTSKPVTELLETLLDAMGILPCG

Sequences:

>Translated_525_residues
MEKRFIRSLLKPSAYPEHTDSVELVQTHVSYIFLTDRFAYKVKKPVDFGFLNFSTIDRRRFYCNEEVRLNRRLCPDIYLG
VEEVRETPGGAGFSGDGRVIDYAVKMKRLPAQRMLDRLLSAGEVSVADIRAIARVIGEFHRRAGRSREIDSYGGADVIRA
NWEENFRQTRQFAGETLSGRALRFIEEWVERFMKENAVIFAQRVAGGFIRDCDGDIHLENICLDSDGSICIFDCIEFNTR
FRYSDTAADIAFLLMDFDVNRRSDLCSPFLDEYIRATGDDGVTDVLDFYKVYRACVRGKVESMRLLDPQIPPADKAAAKE
RSRRYFRLARGYIARRAFPPSLILTCGLTGTGKSYTARELAFDLGLEIVSSDVVRKELTGSAPEEHHWDRYREGIYTESV
TARTYGELFKRAETALNAGRSIIVDATFRRRTDRDLFRQLADRHGSRFHLLHAVCPEEAVRQRLEARRNQCDEPSDGRWD
IYLLQKEEFEAPADDESGTIRIDTSKPVTELLETLLDAMGILPCG
>Mature_525_residues
MEKRFIRSLLKPSAYPEHTDSVELVQTHVSYIFLTDRFAYKVKKPVDFGFLNFSTIDRRRFYCNEEVRLNRRLCPDIYLG
VEEVRETPGGAGFSGDGRVIDYAVKMKRLPAQRMLDRLLSAGEVSVADIRAIARVIGEFHRRAGRSREIDSYGGADVIRA
NWEENFRQTRQFAGETLSGRALRFIEEWVERFMKENAVIFAQRVAGGFIRDCDGDIHLENICLDSDGSICIFDCIEFNTR
FRYSDTAADIAFLLMDFDVNRRSDLCSPFLDEYIRATGDDGVTDVLDFYKVYRACVRGKVESMRLLDPQIPPADKAAAKE
RSRRYFRLARGYIARRAFPPSLILTCGLTGTGKSYTARELAFDLGLEIVSSDVVRKELTGSAPEEHHWDRYREGIYTESV
TARTYGELFKRAETALNAGRSIIVDATFRRRTDRDLFRQLADRHGSRFHLLHAVCPEEAVRQRLEARRNQCDEPSDGRWD
IYLLQKEEFEAPADDESGTIRIDTSKPVTELLETLLDAMGILPCG

Specific function: Some isolated peptides of this protein are able to bind to human U937 monocytoblastic and A549 epithelial cell lines [H]

COG id: COG2187

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011009
- InterPro:   IPR010488 [H]

Pfam domain/function: PF06414 Zeta_toxin [H]

EC number: NA

Molecular weight: Translated: 60133; Mature: 60133

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKRFIRSLLKPSAYPEHTDSVELVQTHVSYIFLTDRFAYKVKKPVDFGFLNFSTIDRRR
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEECHHHHHCCCCCCCCCEEHHHHHHHH
FYCNEEVRLNRRLCPDIYLGVEEVRETPGGAGFSGDGRVIDYAVKMKRLPAQRMLDRLLS
HHCCCHHHHHHHCCCHHHHCHHHHHCCCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHC
AGEVSVADIRAIARVIGEFHRRAGRSREIDSYGGADVIRANWEENFRQTRQFAGETLSGR
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCHH
ALRFIEEWVERFMKENAVIFAQRVAGGFIRDCDGDIHLENICLDSDGSICIFDCIEFNTR
HHHHHHHHHHHHHHCCCEEEEHHHCCCHHCCCCCCEEEEEEEECCCCCEEEEEEECCCCC
FRYSDTAADIAFLLMDFDVNRRSDLCSPFLDEYIRATGDDGVTDVLDFYKVYRACVRGKV
EEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCH
ESMRLLDPQIPPADKAAAKERSRRYFRLARGYIARRAFPPSLILTCGLTGTGKSYTAREL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHH
AFDLGLEIVSSDVVRKELTGSAPEEHHWDRYREGIYTESVTARTYGELFKRAETALNAGR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SIIVDATFRRRTDRDLFRQLADRHGSRFHLLHAVCPEEAVRQRLEARRNQCDEPSDGRWD
EEEEECHHHHCCCHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHHHHCCCCCCCCCEE
IYLLQKEEFEAPADDESGTIRIDTSKPVTELLETLLDAMGILPCG
EEEEEHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKRFIRSLLKPSAYPEHTDSVELVQTHVSYIFLTDRFAYKVKKPVDFGFLNFSTIDRRR
CCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHEEEECHHHHHCCCCCCCCCEEHHHHHHHH
FYCNEEVRLNRRLCPDIYLGVEEVRETPGGAGFSGDGRVIDYAVKMKRLPAQRMLDRLLS
HHCCCHHHHHHHCCCHHHHCHHHHHCCCCCCCCCCCCCEEHHHHHHHHCCHHHHHHHHHC
AGEVSVADIRAIARVIGEFHRRAGRSREIDSYGGADVIRANWEENFRQTRQFAGETLSGR
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCCHH
ALRFIEEWVERFMKENAVIFAQRVAGGFIRDCDGDIHLENICLDSDGSICIFDCIEFNTR
HHHHHHHHHHHHHHCCCEEEEHHHCCCHHCCCCCCEEEEEEEECCCCCEEEEEEECCCCC
FRYSDTAADIAFLLMDFDVNRRSDLCSPFLDEYIRATGDDGVTDVLDFYKVYRACVRGKV
EEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCH
ESMRLLDPQIPPADKAAAKERSRRYFRLARGYIARRAFPPSLILTCGLTGTGKSYTAREL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHHH
AFDLGLEIVSSDVVRKELTGSAPEEHHWDRYREGIYTESVTARTYGELFKRAETALNAGR
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
SIIVDATFRRRTDRDLFRQLADRHGSRFHLLHAVCPEEAVRQRLEARRNQCDEPSDGRWD
EEEEECHHHHCCCHHHHHHHHHCCCCEEEEHHHHCCHHHHHHHHHHHHHCCCCCCCCCEE
IYLLQKEEFEAPADDESGTIRIDTSKPVTELLETLLDAMGILPCG
EEEEEHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]