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The map label for this gene is czcS [H]

Identifier: 78222246

GI number: 78222246

Start: 1142610

End: 1144043

Strand: Direct

Name: czcS [H]

Synonym: Gmet_1027

Alternate gene names: 78222246

Gene position: 1142610-1144043 (Clockwise)

Preceding gene: 78222245

Following gene: 78222247

Centisome position: 28.58

GC content: 56.49

Gene sequence:

>1434_bases
ATGAGAGGGATGCCGAACTCCATACAAGGAAGGCTCTTCCTCTCGATTGTCACGTTTACGTCTCTGTTGTTCATCGCGGT
CGGCGTCTTCATCTACCGCGAGGTAAACGGGATCGTTATCGGCGCGGTTGACAGGACCCTTCACTCCAAAGTACAGGTGA
TTACCGGGCTCCTCCATGAGGAGGACCACACCATTGAACTGGAGTTGGAGGAGGTTGTTTCCGGGGAATATTCCATCCCG
CGGTCCGGGCACTACTTCAAGGTGATGATGAACGGGAAGCTCCTCGCCGCATCGCCTTCGCTGGTTAACGACAGCTTCGA
TCTCGGCGCCGGGGCACCTGAACCTTCTGACGAAGAACTCGGAGAAGATATCTCTACCTCCGTGGGGCCTGCCGGCGAGC
CGATTCGGGTGCTTCGGCACAATCTCAGCGCCTTTGGCATGACGTTCACCGTCTTTGTGGCGGAGAGCCTCAGCGACAGC
CTCGATATGATCAGCACCTTCAGACGTTTTCTCCTTCTGGTCATCCCTTCGGGGATCGTGATCGCCTCTCTCATCTGCCT
GTGGATAGCACGGCAGTCGCTGGCCCCCCTCGCGATATTTTCCGGCAGGATCGAGACCATCACCCACAAAAACCTGGGGG
AAAGGATCGATGCCGAATCGGAGACCCGGGAGTTGGCCGGACTGGCCGGCTCATTCAATAAGATGCTCGATCGGTTGCAA
AAGGTGTTCGAGAGCGAGAAAAGACTGATCGCGGATGCTTCCCATGAGCTCAAGACCCCGGTTTCGGTGATCAAGGTCCA
GTGTGACGTGGTGCTGCAAAGGGAACGGACGCCGGAAGAGTACATTGATGCCATACAGACCATCAGGACGGTCTCCGATA
CTATCAGTATGATCGTGAGGGACCTTCTCTCCCTCGCCCGCCTTGACTCAGGTGTCTTTTCGCAGGGATTTGCGGTCGTA
TCCCTTAACGCCTGCATCGGGAGAGCGCTGGACATGACGAAGCCTTTTGCCGAAAAGAGACACCTTCAGGTTATCGCAGC
ACTGGGAGACGACGTCGCCATCCTGGGAAATGGTGAGAGTCTGACGGAGGCGTTTCTCAACATTCTCGAAAACGGCGTGA
AATATAACAGGGAATACGGCATGTTGGAGATCACCGTCGTCGGGAGTGGCGCGCAAGCGGTTGTCTCCATAAAAGACACC
GGCGTCGGTATGGAAAAGGAGGATCAGGCACGGATATTCGACCGATTCTACCGGGCCGACACTGCACGAAACTCCGATGG
CACCGGGCTCGGCCTCAGTATAGCCAAAGCGATCATCGATGCCCACGGCGGTGCGATCACGCTGGCAAGCGAACCGGGAA
AGGGAGCCACCTTTACCGTAATCTTGCCGGTCAATCACCACACGGTCGAAAAGGGAATACTACCGGGGGCCTGA

Upstream 100 bases:

>100_bases
ACGTCTATGTGAACCACCTGCGAAACAAGCTGGATAAGGGCTTTGACCGACAGCTCATCCACACGGTGCGGGGCGCGGGC
TACATCCTGAAAGGCGATGC

Downstream 100 bases:

>100_bases
CACCGGACAAGTGGCCAGGTGCTCCGAAGACGGCGTGCGTACCGCGCCTTAACCAATGCCTCCCGTTCTCCCGCGAAAAC
CTCTTTACCAGAGCGCAGGA

Product: histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRGMPNSIQGRLFLSIVTFTSLLFIAVGVFIYREVNGIVIGAVDRTLHSKVQVITGLLHEEDHTIELELEEVVSGEYSIP
RSGHYFKVMMNGKLLAASPSLVNDSFDLGAGAPEPSDEELGEDISTSVGPAGEPIRVLRHNLSAFGMTFTVFVAESLSDS
LDMISTFRRFLLLVIPSGIVIASLICLWIARQSLAPLAIFSGRIETITHKNLGERIDAESETRELAGLAGSFNKMLDRLQ
KVFESEKRLIADASHELKTPVSVIKVQCDVVLQRERTPEEYIDAIQTIRTVSDTISMIVRDLLSLARLDSGVFSQGFAVV
SLNACIGRALDMTKPFAEKRHLQVIAALGDDVAILGNGESLTEAFLNILENGVKYNREYGMLEITVVGSGAQAVVSIKDT
GVGMEKEDQARIFDRFYRADTARNSDGTGLGLSIAKAIIDAHGGAITLASEPGKGATFTVILPVNHHTVEKGILPGA

Sequences:

>Translated_477_residues
MRGMPNSIQGRLFLSIVTFTSLLFIAVGVFIYREVNGIVIGAVDRTLHSKVQVITGLLHEEDHTIELELEEVVSGEYSIP
RSGHYFKVMMNGKLLAASPSLVNDSFDLGAGAPEPSDEELGEDISTSVGPAGEPIRVLRHNLSAFGMTFTVFVAESLSDS
LDMISTFRRFLLLVIPSGIVIASLICLWIARQSLAPLAIFSGRIETITHKNLGERIDAESETRELAGLAGSFNKMLDRLQ
KVFESEKRLIADASHELKTPVSVIKVQCDVVLQRERTPEEYIDAIQTIRTVSDTISMIVRDLLSLARLDSGVFSQGFAVV
SLNACIGRALDMTKPFAEKRHLQVIAALGDDVAILGNGESLTEAFLNILENGVKYNREYGMLEITVVGSGAQAVVSIKDT
GVGMEKEDQARIFDRFYRADTARNSDGTGLGLSIAKAIIDAHGGAITLASEPGKGATFTVILPVNHHTVEKGILPGA
>Mature_477_residues
MRGMPNSIQGRLFLSIVTFTSLLFIAVGVFIYREVNGIVIGAVDRTLHSKVQVITGLLHEEDHTIELELEEVVSGEYSIP
RSGHYFKVMMNGKLLAASPSLVNDSFDLGAGAPEPSDEELGEDISTSVGPAGEPIRVLRHNLSAFGMTFTVFVAESLSDS
LDMISTFRRFLLLVIPSGIVIASLICLWIARQSLAPLAIFSGRIETITHKNLGERIDAESETRELAGLAGSFNKMLDRLQ
KVFESEKRLIADASHELKTPVSVIKVQCDVVLQRERTPEEYIDAIQTIRTVSDTISMIVRDLLSLARLDSGVFSQGFAVV
SLNACIGRALDMTKPFAEKRHLQVIAALGDDVAILGNGESLTEAFLNILENGVKYNREYGMLEITVVGSGAQAVVSIKDT
GVGMEKEDQARIFDRFYRADTARNSDGTGLGLSIAKAIIDAHGGAITLASEPGKGATFTVILPVNHHTVEKGILPGA

Specific function: Member of the two-component regulatory system CzcS/CzcR involved in the control of cobalt, zinc and cadmium homeostasis. Probably activates CzcR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=361, Percent_Identity=27.9778393351801, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1788393, Length=279, Percent_Identity=30.4659498207885, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788279, Length=329, Percent_Identity=27.0516717325228, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1788549, Length=280, Percent_Identity=31.4285714285714, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1790346, Length=268, Percent_Identity=29.4776119402985, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790436, Length=218, Percent_Identity=29.8165137614679, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI1789149, Length=264, Percent_Identity=28.030303030303, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786912, Length=219, Percent_Identity=31.9634703196347, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI145693157, Length=249, Percent_Identity=27.3092369477912, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788713, Length=257, Percent_Identity=26.0700389105058, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1789403, Length=286, Percent_Identity=26.5734265734266, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=24.1071428571429, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787894, Length=259, Percent_Identity=24.7104247104247, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1790551, Length=290, Percent_Identity=24.1379310344828, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI48994928, Length=232, Percent_Identity=23.7068965517241, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789808, Length=241, Percent_Identity=23.6514522821577, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI87081816, Length=253, Percent_Identity=23.7154150197628, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87082128, Length=246, Percent_Identity=24.7967479674797, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1790861, Length=278, Percent_Identity=25.8992805755396, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51639; Mature: 51639

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGMPNSIQGRLFLSIVTFTSLLFIAVGVFIYREVNGIVIGAVDRTLHSKVQVITGLLHE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCC
EDHTIELELEEVVSGEYSIPRSGHYFKVMMNGKLLAASPSLVNDSFDLGAGAPEPSDEEL
CCCEEEEEHHHHHCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHH
GEDISTSVGPAGEPIRVLRHNLSAFGMTFTVFVAESLSDSLDMISTFRRFLLLVIPSGIV
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IASLICLWIARQSLAPLAIFSGRIETITHKNLGERIDAESETRELAGLAGSFNKMLDRLQ
HHHHHHHHHHHHCCCCCHHHCCCHHEEHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KVFESEKRLIADASHELKTPVSVIKVQCDVVLQRERTPEEYIDAIQTIRTVSDTISMIVR
HHHHHHHHHHHCCCHHHHCCHHHHEEHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DLLSLARLDSGVFSQGFAVVSLNACIGRALDMTKPFAEKRHLQVIAALGDDVAILGNGES
HHHHHHHHCCCHHHCCEEEEEHHHHHHHHHHHCCHHHHHHHHEEEEECCCCEEEEECCHH
LTEAFLNILENGVKYNREYGMLEITVVGSGAQAVVSIKDTGVGMEKEDQARIFDRFYRAD
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHC
TARNSDGTGLGLSIAKAIIDAHGGAITLASEPGKGATFTVILPVNHHTVEKGILPGA
CCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCHHCCCCCCC
>Mature Secondary Structure
MRGMPNSIQGRLFLSIVTFTSLLFIAVGVFIYREVNGIVIGAVDRTLHSKVQVITGLLHE
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCC
EDHTIELELEEVVSGEYSIPRSGHYFKVMMNGKLLAASPSLVNDSFDLGAGAPEPSDEEL
CCCEEEEEHHHHHCCCCCCCCCCCEEEEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHH
GEDISTSVGPAGEPIRVLRHNLSAFGMTFTVFVAESLSDSLDMISTFRRFLLLVIPSGIV
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
IASLICLWIARQSLAPLAIFSGRIETITHKNLGERIDAESETRELAGLAGSFNKMLDRLQ
HHHHHHHHHHHHCCCCCHHHCCCHHEEHHCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KVFESEKRLIADASHELKTPVSVIKVQCDVVLQRERTPEEYIDAIQTIRTVSDTISMIVR
HHHHHHHHHHHCCCHHHHCCHHHHEEHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHH
DLLSLARLDSGVFSQGFAVVSLNACIGRALDMTKPFAEKRHLQVIAALGDDVAILGNGES
HHHHHHHHCCCHHHCCEEEEEHHHHHHHHHHHCCHHHHHHHHEEEEECCCCEEEEECCHH
LTEAFLNILENGVKYNREYGMLEITVVGSGAQAVVSIKDTGVGMEKEDQARIFDRFYRAD
HHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHC
TARNSDGTGLGLSIAKAIIDAHGGAITLASEPGKGATFTVILPVNHHTVEKGILPGA
CCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9044283 [H]