Click here to switch to the map view.

The map label for this gene is ppnK

Identifier: 78222161

GI number: 78222161

Start: 1044743

End: 1045594

Strand: Direct

Name: ppnK

Synonym: Gmet_0941

Alternate gene names: 78222161

Gene position: 1044743-1045594 (Clockwise)

Preceding gene: 78222157

Following gene: 78222162

Centisome position: 26.14

GC content: 63.38

Gene sequence:

>852_bases
ATGAAAAAAATCGCCATCTTCGCCAAGGTTCACGACCCCCGTTGCCAGGGAGTGGCAGGGGAACTGATCACCTGGCTCGA
GCAGCGGAGGATCGTTCCGCTCGTTGAAGCCCATTTTGCCCGCCATCTGGGGCGATCGGGCGTTACCTCCGAAGAGATTC
CCGACCTCGCTGACATGGCGGTGGTCCTGGGGGGGGACGGCACCCTCATCTCGGCCGCCCGGCTCCTGGGGGGGCGGGAA
ATCCCCATTCTCGGCGTGAACCTGGGGAGCCTCGGCTTTCTGACCGAGGTCACCCTGGACGAGCTCTATCCAGCCCTTGA
AGCATGCCTCGGCGGCGACTACCGGGTTTCGGAGCGGATGATGCTTGCCGCCACCGTGGAGCGGGGCGACGACATCGTCT
TCTCCCACCGGGTTCTGAACGACGCGGTGATCAACAAGGGAGCCCTTGCGCGGATCGTCGACATGGAAAGTCTGGTCAAC
GGCCACTACCTCACCACCTACAAGGCCGACGGCCTCATCATTTCGACCCCCACCGGCTCCACCGGCTACTGCCTCTCGGC
CAATGGCCCCATCGTCCACCCGGACCTGGAATGCCTCACCATCACCCCCATCTGCCCCCATACCCTCACCAACCGTCCCA
TCGTCCTGGAGGCCAGCGCTGAGGTAACCATCAGGCTCATCTCCAAGAATGAGGATGTCTACCTCACCCTCGACGGCCAG
GTGGGGATGGAGCTCAAGTGCGGCGACATCATCCGGGTGCGCCGGGCCGAGCACCGCACGCGCCTCGTTATGTCGCGGAG
CAAGGACTATTTCGAGGTTCTCAGGACCAAGCTCAAGTGGGGTGAGCGGTAG

Upstream 100 bases:

>100_bases
GGAGAAGGCAGTACCCCCAAATGGCGGAATTCCATTGGACATGCCCCCTGATCTATGCTAGTAAATTCCATACTTATACC
ACAATAACAGGGTCGGCACG

Downstream 100 bases:

>100_bases
TGCTCATCGACCTCTCCATCAGAAACTTCGCCATCATCGATACTCTCCATGTTCCGTTCCAGCCGGGGCTTACGGTCTTT
ACCGGCGAGACCGGCGCGGG

Product: ATP-NAD/AcoX kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKKIAIFAKVHDPRCQGVAGELITWLEQRRIVPLVEAHFARHLGRSGVTSEEIPDLADMAVVLGGDGTLISAARLLGGRE
IPILGVNLGSLGFLTEVTLDELYPALEACLGGDYRVSERMMLAATVERGDDIVFSHRVLNDAVINKGALARIVDMESLVN
GHYLTTYKADGLIISTPTGSTGYCLSANGPIVHPDLECLTITPICPHTLTNRPIVLEASAEVTIRLISKNEDVYLTLDGQ
VGMELKCGDIIRVRRAEHRTRLVMSRSKDYFEVLRTKLKWGER

Sequences:

>Translated_283_residues
MKKIAIFAKVHDPRCQGVAGELITWLEQRRIVPLVEAHFARHLGRSGVTSEEIPDLADMAVVLGGDGTLISAARLLGGRE
IPILGVNLGSLGFLTEVTLDELYPALEACLGGDYRVSERMMLAATVERGDDIVFSHRVLNDAVINKGALARIVDMESLVN
GHYLTTYKADGLIISTPTGSTGYCLSANGPIVHPDLECLTITPICPHTLTNRPIVLEASAEVTIRLISKNEDVYLTLDGQ
VGMELKCGDIIRVRRAEHRTRLVMSRSKDYFEVLRTKLKWGER
>Mature_283_residues
MKKIAIFAKVHDPRCQGVAGELITWLEQRRIVPLVEAHFARHLGRSGVTSEEIPDLADMAVVLGGDGTLISAARLLGGRE
IPILGVNLGSLGFLTEVTLDELYPALEACLGGDYRVSERMMLAATVERGDDIVFSHRVLNDAVINKGALARIVDMESLVN
GHYLTTYKADGLIISTPTGSTGYCLSANGPIVHPDLECLTITPICPHTLTNRPIVLEASAEVTIRLISKNEDVYLTLDGQ
VGMELKCGDIIRVRRAEHRTRLVMSRSKDYFEVLRTKLKWGER

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=262, Percent_Identity=31.2977099236641, Blast_Score=124, Evalue=8e-29,
Organism=Escherichia coli, GI1788968, Length=286, Percent_Identity=34.6153846153846, Blast_Score=182, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320794, Length=242, Percent_Identity=32.6446280991736, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322509, Length=242, Percent_Identity=30.5785123966942, Blast_Score=127, Evalue=3e-30,
Organism=Saccharomyces cerevisiae, GI6325068, Length=212, Percent_Identity=32.5471698113208, Blast_Score=123, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28573828, Length=254, Percent_Identity=33.4645669291339, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28573832, Length=254, Percent_Identity=33.4645669291339, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28573826, Length=254, Percent_Identity=33.4645669291339, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI28573830, Length=254, Percent_Identity=33.4645669291339, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI161077047, Length=254, Percent_Identity=33.4645669291339, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI20129957, Length=279, Percent_Identity=29.7491039426523, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281363323, Length=279, Percent_Identity=29.7491039426523, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI281363321, Length=279, Percent_Identity=29.7491039426523, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653422, Length=279, Percent_Identity=29.7491039426523, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24653424, Length=279, Percent_Identity=29.7491039426523, Blast_Score=115, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_GEOMG (Q39X41)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383908.1
- ProteinModelPortal:   Q39X41
- SMR:   Q39X41
- STRING:   Q39X41
- GeneID:   3738511
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0941
- NMPDR:   fig|269799.3.peg.1016
- eggNOG:   COG0061
- HOGENOM:   HBG713904
- OMA:   FESFMID
- PhylomeDB:   Q39X41
- ProtClustDB:   CLSK828714
- BioCyc:   GMET269799:GMET_0941-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 31072; Mature: 31072

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIAIFAKVHDPRCQGVAGELITWLEQRRIVPLVEAHFARHLGRSGVTSEEIPDLADMA
CCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHEE
VVLGGDGTLISAARLLGGREIPILGVNLGSLGFLTEVTLDELYPALEACLGGDYRVSERM
EEECCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCEE
MLAATVERGDDIVFSHRVLNDAVINKGALARIVDMESLVNGHYLTTYKADGLIISTPTGS
EEEEEECCCCCEEEEHHHHHHHHHCCCHHHHHEEHHHHHCCCEEEEEECCCEEEECCCCC
TGYCLSANGPIVHPDLECLTITPICPHTLTNRPIVLEASAEVTIRLISKNEDVYLTLDGQ
CCEEEECCCCEECCCCCEEEECCCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEEECCC
VGMELKCGDIIRVRRAEHRTRLVMSRSKDYFEVLRTKLKWGER
CCCEEECCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKIAIFAKVHDPRCQGVAGELITWLEQRRIVPLVEAHFARHLGRSGVTSEEIPDLADMA
CCEEEEEEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCHHHEE
VVLGGDGTLISAARLLGGREIPILGVNLGSLGFLTEVTLDELYPALEACLGGDYRVSERM
EEECCCCHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEECCCEE
MLAATVERGDDIVFSHRVLNDAVINKGALARIVDMESLVNGHYLTTYKADGLIISTPTGS
EEEEEECCCCCEEEEHHHHHHHHHCCCHHHHHEEHHHHHCCCEEEEEECCCEEEECCCCC
TGYCLSANGPIVHPDLECLTITPICPHTLTNRPIVLEASAEVTIRLISKNEDVYLTLDGQ
CCEEEECCCCEECCCCCEEEECCCCCCCCCCCCEEEEECCEEEEEEEECCCCEEEEECCC
VGMELKCGDIIRVRRAEHRTRLVMSRSKDYFEVLRTKLKWGER
CCCEEECCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA