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The map label for this gene is gph [H]

Identifier: 78222157

GI number: 78222157

Start: 1039143

End: 1039763

Strand: Direct

Name: gph [H]

Synonym: Gmet_0937

Alternate gene names: 78222157

Gene position: 1039143-1039763 (Clockwise)

Preceding gene: 78222156

Following gene: 78222161

Centisome position: 26.0

GC content: 61.03

Gene sequence:

>621_bases
GTGCGTTACAAGGCAGTAATCTACGATTGTGATGGGGTGATGTTCGACTCCTTCGAGGCGAATCTCGCCTTCTACCAGCG
GATCATGGGGATGATGGGGCGCAAGCCCCTCGACCGGTCCGACGAGGAGCAGATGCGCATCCTCCATACCTATGCTAACC
GGGAGGTCCTCGCTCATTTTTTCCCCGACCCGAGGGAGTGGGAAGAGGCGGTCCGCTGTGCCGGCCGGATCGACTACCGG
GACCTCGTCCCTCTGATGGTCATGGAACAGGGGTTTCGCGAAACCCTCGATGTGCTCAAGGGTCGTGTTGAGCTGGCCGT
CTGCACCAATCGGTCAACCTCCATGGACACCGTTCTGGAAAGCTTCGACCTCGCTTCCTATTTCGGCCTCGTCATGACCG
CCTCGAAGGTGGCCAACCCGAAGCCCCACCCGGAGCCGCTTCTGAGGGTTGTTGAACACTTCGGCATCAAACCCGGTGAG
GCCCTCTTTGTGGGGGACTCGGCCGTGGACTCCATGGCCGCGGAAGCAGCCGGCGTGCCTTTTGTCGCCTACAAGGCAGA
CCTCCCCGCCATGACGTGCATCGACCACCATGGCGATCTGGTCGCCCTTGTGCGATCCTGA

Upstream 100 bases:

>100_bases
ATCTCCTCCCCGTGGAGTGGGAGGAGTATCTGGATTCGTTGGAAAGCGAACCGTCCGACGGTGAAGGGTAACCTCCGCCA
GCGGGCGGCATGGGGTAAGC

Downstream 100 bases:

>100_bases
ACCGCTCCCACATGGTTCCCTAACACTAAACACCTAACACCTCTGCCAGCCGCAATACCTCGTCCTGGGGGCGTTTCTCG
CCTTTGATGACGTCTTCCAG

Product: HAD family hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MRYKAVIYDCDGVMFDSFEANLAFYQRIMGMMGRKPLDRSDEEQMRILHTYANREVLAHFFPDPREWEEAVRCAGRIDYR
DLVPLMVMEQGFRETLDVLKGRVELAVCTNRSTSMDTVLESFDLASYFGLVMTASKVANPKPHPEPLLRVVEHFGIKPGE
ALFVGDSAVDSMAAEAAGVPFVAYKADLPAMTCIDHHGDLVALVRS

Sequences:

>Translated_206_residues
MRYKAVIYDCDGVMFDSFEANLAFYQRIMGMMGRKPLDRSDEEQMRILHTYANREVLAHFFPDPREWEEAVRCAGRIDYR
DLVPLMVMEQGFRETLDVLKGRVELAVCTNRSTSMDTVLESFDLASYFGLVMTASKVANPKPHPEPLLRVVEHFGIKPGE
ALFVGDSAVDSMAAEAAGVPFVAYKADLPAMTCIDHHGDLVALVRS
>Mature_206_residues
MRYKAVIYDCDGVMFDSFEANLAFYQRIMGMMGRKPLDRSDEEQMRILHTYANREVLAHFFPDPREWEEAVRCAGRIDYR
DLVPLMVMEQGFRETLDVLKGRVELAVCTNRSTSMDTVLESFDLASYFGLVMTASKVANPKPHPEPLLRVVEHFGIKPGE
ALFVGDSAVDSMAAEAAGVPFVAYKADLPAMTCIDHHGDLVALVRS

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=92, Percent_Identity=45.6521739130435, Blast_Score=77, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23157; Mature: 23157

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
7.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYKAVIYDCDGVMFDSFEANLAFYQRIMGMMGRKPLDRSDEEQMRILHTYANREVLAHF
CCCEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHC
FPDPREWEEAVRCAGRIDYRDLVPLMVMEQGFRETLDVLKGRVELAVCTNRSTSMDTVLE
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHH
SFDLASYFGLVMTASKVANPKPHPEPLLRVVEHFGIKPGEALFVGDSAVDSMAAEAAGVP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCC
FVAYKADLPAMTCIDHHGDLVALVRS
EEEEECCCCCEEEECCCCCEEEEECC
>Mature Secondary Structure
MRYKAVIYDCDGVMFDSFEANLAFYQRIMGMMGRKPLDRSDEEQMRILHTYANREVLAHF
CCCEEEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCHHHHHHC
FPDPREWEEAVRCAGRIDYRDLVPLMVMEQGFRETLDVLKGRVELAVCTNRSTSMDTVLE
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHH
SFDLASYFGLVMTASKVANPKPHPEPLLRVVEHFGIKPGEALFVGDSAVDSMAAEAAGVP
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHCCCC
FVAYKADLPAMTCIDHHGDLVALVRS
EEEEECCCCCEEEECCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]