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The map label for this gene is aprN [H]

Identifier: 78222151

GI number: 78222151

Start: 1027000

End: 1028502

Strand: Direct

Name: aprN [H]

Synonym: Gmet_0931

Alternate gene names: 78222151

Gene position: 1027000-1028502 (Clockwise)

Preceding gene: 78222150

Following gene: 78222152

Centisome position: 25.69

GC content: 55.29

Gene sequence:

>1503_bases
ATGAACATACTACGCACCCTTCTTGTCGCTGTTATGGGGGCGATGCTTTGCTTACCACTTGATTCGTTTGCTGCCACGAA
GAAGGTGATCGTCGGATTCACTCGCGCGCCAAAAGATCATGAAAAAAATCTGGTGTATGGCGCAAGAGGCCGAATCCACC
GGACTCACCAGCACATCCGTGCCATTTCCGCGGAACTTCCCGAGGAAGAGATAGCGCGTCTGAAGCAGGACCCTTCGGTT
GCTTATGTTGTTGATAATATTAAATTTACTCTGATTCGACCGGCAGAGCCGCTCAGTGTCCCGTCACCCGAATATGTGGA
TTCGTGGGGTGTTCTGCGGATCGGCTCCAATGTTGCCGCCTCCCGTGGATATAAAGGGGCGGGAATCAAGGTCGCCATTG
TTGACTCCGGAATTGACTACAACCATCCCGACCTCAAGGACAACTATCGGGGTGGATACAATTTTGTAAACAATACAGCA
GATCCCTTTGACGACGATGCCCAGAGCCATGGAACCCATGTGGCGGGGATCATCGCGGCCCGGGACAACGGCACCGGCGT
TGTCGGTGTGGCGCCGGAAGCATCCCTCTACGCGGTAAAGGTCTTCAGTGCCACCGCCGGGGGGGACATGGATACCGTCG
TTGCCGGGATTGAGTGGGCCATTGACAACAAGATGGATGTGATCAACCTCAGCATCGGGTATTCCGGCGACATCTATTCC
ATCTATCCCGACATTTTCAAGCCTCTCAAGGATGTCTGCGACCGGGCCTACCAGGCAGGCATCGTTCTGGTTGCGGCAAC
AGGAAACGACAACCGGGAAACTATTTCCGTTCCGGCGGCGTTCGATTCCGTCATTGCCGTTGCGGCCACTGACCAGAATG
ACCAGCGTGCCGTCTTTAACACTGTCGCTGCATCCAGTTATGGTGCAAAAGTGGAACTGGCCGCGCCGGGCACCTATATC
AAATCCACGGTCAGCGGTGGTGGATACGCACTCCTGAGCGGCACTTCCCAGGCCTCACCCCATGTTGCTGGAGCGGCTGC
GGTTCTTCTTTCCAGCGGCATTGCCGACGCAAACGGTAACGGCAGTCGTGCCGACGAGGTACGGGCAAGGCTGGACGCAA
CCGCCAAGGATTTGGGGGATCCAGGGCGTGACAAGTACTTCGGCTGGGGGTTGGTTGACCTTTCTGCGTCTACGGACCAG
ACCTACAAGTTACATATCGAGAAGAAAAAAGGCATGCCGAAGCTCAGCACACAATCCATAACCCTCGACAAGGGGAGCTA
TTCGTTGCAGGTCAGGAATAACGGCTTGGAGCGGCTGGCGGTAAAGTCTGTCGTTGGAAGCTGCAAGGAATTCGAGCGGG
TCTTTGCCACCGGCTTCACATCTCCTCTTGAGGAACTGAATGCCAGTGTGACTCTCGATGGCTCTCAAACGACGCTTGTT
TTTACCCCGTCCGGTGACATTGGTTCCTCAGCTGACATCACCATCACGAAACTCGCCCGGTAA

Upstream 100 bases:

>100_bases
AGCGGTAATTTTTGATGTTCCGGGATAGGGGAGGGAGTTTCTCCCCTATCCCACTTTTTACCTGTTTACCGGCTGCTTCC
CATTGATTAAGAGGTACACC

Downstream 100 bases:

>100_bases
CGGGTGACGCATAGAGGAAAAATGAGATTAAATCGTTCGCTAACATTTGGATTATTCCTTTTGGGAGTGATGTTCACTCT
TCATCTGCTTCAGACGCCTG

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: Nattokinase [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNILRTLLVAVMGAMLCLPLDSFAATKKVIVGFTRAPKDHEKNLVYGARGRIHRTHQHIRAISAELPEEEIARLKQDPSV
AYVVDNIKFTLIRPAEPLSVPSPEYVDSWGVLRIGSNVAASRGYKGAGIKVAIVDSGIDYNHPDLKDNYRGGYNFVNNTA
DPFDDDAQSHGTHVAGIIAARDNGTGVVGVAPEASLYAVKVFSATAGGDMDTVVAGIEWAIDNKMDVINLSIGYSGDIYS
IYPDIFKPLKDVCDRAYQAGIVLVAATGNDNRETISVPAAFDSVIAVAATDQNDQRAVFNTVAASSYGAKVELAAPGTYI
KSTVSGGGYALLSGTSQASPHVAGAAAVLLSSGIADANGNGSRADEVRARLDATAKDLGDPGRDKYFGWGLVDLSASTDQ
TYKLHIEKKKGMPKLSTQSITLDKGSYSLQVRNNGLERLAVKSVVGSCKEFERVFATGFTSPLEELNASVTLDGSQTTLV
FTPSGDIGSSADITITKLAR

Sequences:

>Translated_500_residues
MNILRTLLVAVMGAMLCLPLDSFAATKKVIVGFTRAPKDHEKNLVYGARGRIHRTHQHIRAISAELPEEEIARLKQDPSV
AYVVDNIKFTLIRPAEPLSVPSPEYVDSWGVLRIGSNVAASRGYKGAGIKVAIVDSGIDYNHPDLKDNYRGGYNFVNNTA
DPFDDDAQSHGTHVAGIIAARDNGTGVVGVAPEASLYAVKVFSATAGGDMDTVVAGIEWAIDNKMDVINLSIGYSGDIYS
IYPDIFKPLKDVCDRAYQAGIVLVAATGNDNRETISVPAAFDSVIAVAATDQNDQRAVFNTVAASSYGAKVELAAPGTYI
KSTVSGGGYALLSGTSQASPHVAGAAAVLLSSGIADANGNGSRADEVRARLDATAKDLGDPGRDKYFGWGLVDLSASTDQ
TYKLHIEKKKGMPKLSTQSITLDKGSYSLQVRNNGLERLAVKSVVGSCKEFERVFATGFTSPLEELNASVTLDGSQTTLV
FTPSGDIGSSADITITKLAR
>Mature_500_residues
MNILRTLLVAVMGAMLCLPLDSFAATKKVIVGFTRAPKDHEKNLVYGARGRIHRTHQHIRAISAELPEEEIARLKQDPSV
AYVVDNIKFTLIRPAEPLSVPSPEYVDSWGVLRIGSNVAASRGYKGAGIKVAIVDSGIDYNHPDLKDNYRGGYNFVNNTA
DPFDDDAQSHGTHVAGIIAARDNGTGVVGVAPEASLYAVKVFSATAGGDMDTVVAGIEWAIDNKMDVINLSIGYSGDIYS
IYPDIFKPLKDVCDRAYQAGIVLVAATGNDNRETISVPAAFDSVIAVAATDQNDQRAVFNTVAASSYGAKVELAAPGTYI
KSTVSGGGYALLSGTSQASPHVAGAAAVLLSSGIADANGNGSRADEVRARLDATAKDLGDPGRDKYFGWGLVDLSASTDQ
TYKLHIEKKKGMPKLSTQSITLDKGSYSLQVRNNGLERLAVKSVVGSCKEFERVFATGFTSPLEELNASVTLDGSQTTLV
FTPSGDIGSSADITITKLAR

Specific function: Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Subtilisin NAT also has fibrinolytic activity [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

Organism=Homo sapiens, GI4506775, Length=249, Percent_Identity=32.5301204819277, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI31317307, Length=290, Percent_Identity=27.5862068965517, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI4505579, Length=157, Percent_Identity=33.1210191082803, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI20336180, Length=375, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI4505577, Length=375, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI27894285, Length=375, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI20336184, Length=375, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI20336182, Length=375, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=5e-11,
Organism=Homo sapiens, GI20336190, Length=375, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=7e-11,
Organism=Homo sapiens, GI20336188, Length=375, Percent_Identity=26.6666666666667, Blast_Score=66, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI71987206, Length=264, Percent_Identity=28.7878787878788, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320775, Length=258, Percent_Identity=33.7209302325581, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319893, Length=272, Percent_Identity=31.25, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6324576, Length=320, Percent_Identity=27.8125, Blast_Score=97, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6324091, Length=322, Percent_Identity=27.9503105590062, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550681, Length=306, Percent_Identity=29.0849673202614, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17136192, Length=265, Percent_Identity=29.0566037735849, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500
- InterPro:   IPR009020
- InterPro:   IPR010259 [H]

Pfam domain/function: PF05922 Inhibitor_I9; PF00082 Peptidase_S8 [H]

EC number: =3.4.21.62 [H]

Molecular weight: Translated: 52924; Mature: 52924

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00136 SUBTILASE_ASP ; PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILRTLLVAVMGAMLCLPLDSFAATKKVIVGFTRAPKDHEKNLVYGARGRIHRTHQHIR
CCHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHH
AISAELPEEEIARLKQDPSVAYVVDNIKFTLIRPAEPLSVPSPEYVDSWGVLRIGSNVAA
HHHHCCCHHHHHHHHCCCCEEEEEECEEEEEECCCCCCCCCCCCHHCCCCEEEECCCHHH
SRGYKGAGIKVAIVDSGIDYNHPDLKDNYRGGYNFVNNTADPFDDDAQSHGTHVAGIIAA
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEEEE
RDNGTGVVGVAPEASLYAVKVFSATAGGDMDTVVAGIEWAIDNKMDVINLSIGYSGDIYS
CCCCCEEEEECCCCCEEEEEEEEECCCCCHHHHEEEEEEEECCCEEEEEEEECCCCCEEE
IYPDIFKPLKDVCDRAYQAGIVLVAATGNDNRETISVPAAFDSVIAVAATDQNDQRAVFN
ECHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCHHHHH
TVAASSYGAKVELAAPGTYIKSTVSGGGYALLSGTSQASPHVAGAAAVLLSSGIADANGN
HHHHCCCCCEEEEECCCCCHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCC
GSRADEVRARLDATAKDLGDPGRDKYFGWGLVDLSASTDQTYKLHIEKKKGMPKLSTQSI
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCEE
TLDKGSYSLQVRNNGLERLAVKSVVGSCKEFERVFATGFTSPLEELNASVTLDGSQTTLV
EEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCEEEE
FTPSGDIGSSADITITKLAR
EECCCCCCCCCCEEEEEECC
>Mature Secondary Structure
MNILRTLLVAVMGAMLCLPLDSFAATKKVIVGFTRAPKDHEKNLVYGARGRIHRTHQHIR
CCHHHHHHHHHHHHHHHCCCHHHHHCCEEEEEECCCCCCCCCCEEEECCCCHHHHHHHHH
AISAELPEEEIARLKQDPSVAYVVDNIKFTLIRPAEPLSVPSPEYVDSWGVLRIGSNVAA
HHHHCCCHHHHHHHHCCCCEEEEEECEEEEEECCCCCCCCCCCCHHCCCCEEEECCCHHH
SRGYKGAGIKVAIVDSGIDYNHPDLKDNYRGGYNFVNNTADPFDDDAQSHGTHVAGIIAA
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEEEE
RDNGTGVVGVAPEASLYAVKVFSATAGGDMDTVVAGIEWAIDNKMDVINLSIGYSGDIYS
CCCCCEEEEECCCCCEEEEEEEEECCCCCHHHHEEEEEEEECCCEEEEEEEECCCCCEEE
IYPDIFKPLKDVCDRAYQAGIVLVAATGNDNRETISVPAAFDSVIAVAATDQNDQRAVFN
ECHHHHHHHHHHHHHHHHCCEEEEEEECCCCCEEEECCCCCCCEEEEEECCCCCCHHHHH
TVAASSYGAKVELAAPGTYIKSTVSGGGYALLSGTSQASPHVAGAAAVLLSSGIADANGN
HHHHCCCCCEEEEECCCCCHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCC
GSRADEVRARLDATAKDLGDPGRDKYFGWGLVDLSASTDQTYKLHIEKKKGMPKLSTQSI
CCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCEEEEEEECCCCCCCCCCCEE
TLDKGSYSLQVRNNGLERLAVKSVVGSCKEFERVFATGFTSPLEELNASVTLDGSQTTLV
EEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCEEEE
FTPSGDIGSSADITITKLAR
EECCCCCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1369081 [H]