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The map label for this gene is pilJ [H]

Identifier: 78222019

GI number: 78222019

Start: 879101

End: 880267

Strand: Reverse

Name: pilJ [H]

Synonym: Gmet_0799

Alternate gene names: 78222019

Gene position: 880267-879101 (Counterclockwise)

Preceding gene: 78222020

Following gene: 78222018

Centisome position: 22.02

GC content: 58.87

Gene sequence:

>1167_bases
TTGAACCTTTATCTTCACCTGAATATCCGAACACGGATCGTGCTGCTTTGCATCTGCTACAGCACCTGCATCATCCTTGC
AGTCATAGCTGGGCGCTCGTTATCGACGGCAGAGGCAATCGCATCCACAACCGTTTTTGTGCTGCTCGGGGCCTTATTCA
GCTCCCTCCTTTTCCGTACGGTCAACGATGCGCTCAAGCGCATCCAGGGCTACCTGGCCACATTGACCGGGGGAGACCTG
ATGCAAACTATTGCGCCCAAGCGCAACAACGAGATCAGCAGTATCATCCGTTCCATCGCCAGCCTTCAGGCAACCATCCG
CGAAATCGTCTCCCAGATATCGCAGACGTCGGAAGAGGTTGCCATGGCCTCGCGGCAACTCCAGTCCAACGCCGACCAGA
TTGCCGCCGGGACGGACAATGCCGCCTCCCAGACCAACACAGTGGCCGTAGCTAGTGAAGAGATGGCAGCCACTTCAGGC
GACATCGCTAACAATTGCATGAGAGCAGCGGAAAACTCCACCCGCGCTGTCAACACCGCCCGATCGGGAGCCGAAGTTGT
CCGCCAGGCCACGGATTGCATGGAGCGCATCGCCAGCCGGGTTAGAGACGCGGCAAAGACCGTCGAAGAGTTGGGCTCCC
GGTCCGATCAGATCGGACAGATCATTGGGACAATCCAGGACATCGCCGATCAGACAAACCTGCTGGCGCTCAACGCGGCC
ATCGAAGCCGCCCGAGCGGGGGAGCAGGGCCGAGGGTTCGCTGTTGTGGCAGACGAAGTCCGTGCCCTCGCGGAGCGCAC
CACTCGCGCCACCCGCGAAATTGGTGAAATGATTAAGACCATTCAGAATGAAACAAAGGGAGCTGTTTCAGCAATCGACG
AAGGAGTGGCAGAGGTCGAGAGGGGGGCCGAATACTCGGATAAGTCCGGCCAGTCTCTGGAACAGATACTCCAACAGATC
AACGACGTCACCATGCAGATCAACCAGATCGCCACCGCCGCAGAGCAACAGACCGCCACGACCGGGGAAATATCATCCAA
CATCCAGCAGATCACCGCGGTGGTCCAGCAGACCGCCCGGGGTGCCACGGAAACAGCCACCGCGGCGGCCACGCTTTCAA
GACAGGCTGACGAACTTCAGGGCCTGGTGGGGCATTTCAGACTGTGA

Upstream 100 bases:

>100_bases
CAAGGTGATCAAAAGAATGGCCTACGGCTTCCGGGATCACGAATACTTCTTTCTCAAGATCAGAGCCGCTTTCCCCGGAA
TTCCCGGATGAACCTTTTTT

Downstream 100 bases:

>100_bases
GATTCCGTTTAAGAGTTATGCACATCCCCTTGTGATGGCATGGAAAAATTGGTGAATACTATTGGGGCTCTGGTTTATGC
GACCAGGGCTTTTTTTCTAA

Product: methyl-accepting chemotaxis sensory transducer

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNLYLHLNIRTRIVLLCICYSTCIILAVIAGRSLSTAEAIASTTVFVLLGALFSSLLFRTVNDALKRIQGYLATLTGGDL
MQTIAPKRNNEISSIIRSIASLQATIREIVSQISQTSEEVAMASRQLQSNADQIAAGTDNAASQTNTVAVASEEMAATSG
DIANNCMRAAENSTRAVNTARSGAEVVRQATDCMERIASRVRDAAKTVEELGSRSDQIGQIIGTIQDIADQTNLLALNAA
IEAARAGEQGRGFAVVADEVRALAERTTRATREIGEMIKTIQNETKGAVSAIDEGVAEVERGAEYSDKSGQSLEQILQQI
NDVTMQINQIATAAEQQTATTGEISSNIQQITAVVQQTARGATETATAAATLSRQADELQGLVGHFRL

Sequences:

>Translated_388_residues
MNLYLHLNIRTRIVLLCICYSTCIILAVIAGRSLSTAEAIASTTVFVLLGALFSSLLFRTVNDALKRIQGYLATLTGGDL
MQTIAPKRNNEISSIIRSIASLQATIREIVSQISQTSEEVAMASRQLQSNADQIAAGTDNAASQTNTVAVASEEMAATSG
DIANNCMRAAENSTRAVNTARSGAEVVRQATDCMERIASRVRDAAKTVEELGSRSDQIGQIIGTIQDIADQTNLLALNAA
IEAARAGEQGRGFAVVADEVRALAERTTRATREIGEMIKTIQNETKGAVSAIDEGVAEVERGAEYSDKSGQSLEQILQQI
NDVTMQINQIATAAEQQTATTGEISSNIQQITAVVQQTARGATETATAAATLSRQADELQGLVGHFRL
>Mature_388_residues
MNLYLHLNIRTRIVLLCICYSTCIILAVIAGRSLSTAEAIASTTVFVLLGALFSSLLFRTVNDALKRIQGYLATLTGGDL
MQTIAPKRNNEISSIIRSIASLQATIREIVSQISQTSEEVAMASRQLQSNADQIAAGTDNAASQTNTVAVASEEMAATSG
DIANNCMRAAENSTRAVNTARSGAEVVRQATDCMERIASRVRDAAKTVEELGSRSDQIGQIIGTIQDIADQTNLLALNAA
IEAARAGEQGRGFAVVADEVRALAERTTRATREIGEMIKTIQNETKGAVSAIDEGVAEVERGAEYSDKSGQSLEQILQQI
NDVTMQINQIATAAEQQTATTGEISSNIQQITAVVQQTARGATETATAAATLSRQADELQGLVGHFRL

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI1788194, Length=354, Percent_Identity=33.0508474576271, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788195, Length=296, Percent_Identity=31.7567567567568, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1787690, Length=333, Percent_Identity=32.7327327327327, Blast_Score=128, Evalue=5e-31,
Organism=Escherichia coli, GI2367378, Length=328, Percent_Identity=30.1829268292683, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1789453, Length=296, Percent_Identity=28.3783783783784, Blast_Score=108, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 41234; Mature: 41234

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLYLHLNIRTRIVLLCICYSTCIILAVIAGRSLSTAEAIASTTVFVLLGALFSSLLFRT
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VNDALKRIQGYLATLTGGDLMQTIAPKRNNEISSIIRSIASLQATIREIVSQISQTSEEV
HHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMASRQLQSNADQIAAGTDNAASQTNTVAVASEEMAATSGDIANNCMRAAENSTRAVNTA
HHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
RSGAEVVRQATDCMERIASRVRDAAKTVEELGSRSDQIGQIIGTIQDIADQTNLLALNAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IEAARAGEQGRGFAVVADEVRALAERTTRATREIGEMIKTIQNETKGAVSAIDEGVAEVE
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGAEYSDKSGQSLEQILQQINDVTMQINQIATAAEQQTATTGEISSNIQQITAVVQQTAR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
GATETATAAATLSRQADELQGLVGHFRL
CCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLYLHLNIRTRIVLLCICYSTCIILAVIAGRSLSTAEAIASTTVFVLLGALFSSLLFRT
CCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VNDALKRIQGYLATLTGGDLMQTIAPKRNNEISSIIRSIASLQATIREIVSQISQTSEEV
HHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AMASRQLQSNADQIAAGTDNAASQTNTVAVASEEMAATSGDIANNCMRAAENSTRAVNTA
HHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHCCCHHHHHHH
RSGAEVVRQATDCMERIASRVRDAAKTVEELGSRSDQIGQIIGTIQDIADQTNLLALNAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IEAARAGEQGRGFAVVADEVRALAERTTRATREIGEMIKTIQNETKGAVSAIDEGVAEVE
HHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RGAEYSDKSGQSLEQILQQINDVTMQINQIATAAEQQTATTGEISSNIQQITAVVQQTAR
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHC
GATETATAAATLSRQADELQGLVGHFRL
CCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]