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The map label for this gene is rhtB [H]

Identifier: 78222018

GI number: 78222018

Start: 878243

End: 878863

Strand: Reverse

Name: rhtB [H]

Synonym: Gmet_0798

Alternate gene names: 78222018

Gene position: 878863-878243 (Counterclockwise)

Preceding gene: 78222019

Following gene: 78222017

Centisome position: 21.99

GC content: 65.54

Gene sequence:

>621_bases
ATGACCCTCTATACCCTGCTCTGTTTCCTCGGCGCCTCCATCGCCCTGACCGTGGCCCCCGGCCCTGACAACATCTTCGT
CATGACCCAGGGGGTCGCCCGGGGGAGGAAGCCGGCCATCGTCACGGCCCTCGGCATGTGCAGCGGCGTCACCGTCCACA
CCACGGCCGCGGCCTTCGGGATCTCGGCGGTGTTCTATTCCTCGGCCATTGCCTTCACGGTGGTGAAGTCCGCCGGCGCG
GCATACCTCCTCTACCTGGCCTTCAAAACCCTGAAGGAACGGTCCGCCATCAGGCTCTCGGCGGCCGACGACCTCCCCGC
GGCGGCCCTGTTCCGGCGGGGATTCATCATGAACGTGCTCAACCCCAAGGTGGCCATGTTCTTCCTGGCGTTCCTCCCCC
AGTTCGTGGCCCCCGCCGCCGGCCGCGTCCCCCTCCAGATGCTGCTCTTGGGACTCATATTCATGGTGCAGTCCGTGGTA
ATCTTCTGCCTGCTCGGCTACTTCGCCGGGAGCATCGGCAGCTTCATCCTAGCCCGCCCCCGGATCGCACGGTATTTTGA
CTGGTTGACCGCGGGGGTTTTCCTGTCCCTGGGGGTAAGGCTGGCCCTGGCGGAACGGTAA

Upstream 100 bases:

>100_bases
ACAAGGAGGGGGAACCCTCAATAGCTTTGTGCCGCGACACGATTCGGAAACAAGCACTTTAACAGCGCGCTCCGACTGTC
TTCCGCGGAAGGGCCCCCCC

Downstream 100 bases:

>100_bases
TTTTCCCCTGTTCCGGAAATGAATCCTCCCCCTCCAGGGATTTAAATAATAATATTTTCATAAAAAATGGTTTCATGATA
CAGAAAGACGCCTTGCGATT

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 205

Protein sequence:

>206_residues
MTLYTLLCFLGASIALTVAPGPDNIFVMTQGVARGRKPAIVTALGMCSGVTVHTTAAAFGISAVFYSSAIAFTVVKSAGA
AYLLYLAFKTLKERSAIRLSAADDLPAAALFRRGFIMNVLNPKVAMFFLAFLPQFVAPAAGRVPLQMLLLGLIFMVQSVV
IFCLLGYFAGSIGSFILARPRIARYFDWLTAGVFLSLGVRLALAER

Sequences:

>Translated_206_residues
MTLYTLLCFLGASIALTVAPGPDNIFVMTQGVARGRKPAIVTALGMCSGVTVHTTAAAFGISAVFYSSAIAFTVVKSAGA
AYLLYLAFKTLKERSAIRLSAADDLPAAALFRRGFIMNVLNPKVAMFFLAFLPQFVAPAAGRVPLQMLLLGLIFMVQSVV
IFCLLGYFAGSIGSFILARPRIARYFDWLTAGVFLSLGVRLALAER
>Mature_205_residues
TLYTLLCFLGASIALTVAPGPDNIFVMTQGVARGRKPAIVTALGMCSGVTVHTTAAAFGISAVFYSSAIAFTVVKSAGAA
YLLYLAFKTLKERSAIRLSAADDLPAAALFRRGFIMNVLNPKVAMFFLAFLPQFVAPAAGRVPLQMLLLGLIFMVQSVVI
FCLLGYFAGSIGSFILARPRIARYFDWLTAGVFLSLGVRLALAER

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=207, Percent_Identity=30.4347826086957, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1788099, Length=202, Percent_Identity=28.7128712871287, Blast_Score=77, Evalue=6e-16,
Organism=Escherichia coli, GI1786522, Length=115, Percent_Identity=29.5652173913043, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI48994978, Length=215, Percent_Identity=25.5813953488372, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22000; Mature: 21868

Theoretical pI: Translated: 10.67; Mature: 10.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLYTLLCFLGASIALTVAPGPDNIFVMTQGVARGRKPAIVTALGMCSGVTVHTTAAAFG
CHHHHHHHHHCCCEEEEEECCCCCEEEEECCHHCCCCCHHHHHHHHHCCCCHHHHHHHHH
ISAVFYSSAIAFTVVKSAGAAYLLYLAFKTLKERSAIRLSAADDLPAAALFRRGFIMNVL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
NPKVAMFFLAFLPQFVAPAAGRVPLQMLLLGLIFMVQSVVIFCLLGYFAGSIGSFILARP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RIARYFDWLTAGVFLSLGVRLALAER
HHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLYTLLCFLGASIALTVAPGPDNIFVMTQGVARGRKPAIVTALGMCSGVTVHTTAAAFG
HHHHHHHHHCCCEEEEEECCCCCEEEEECCHHCCCCCHHHHHHHHHCCCCHHHHHHHHH
ISAVFYSSAIAFTVVKSAGAAYLLYLAFKTLKERSAIRLSAADDLPAAALFRRGFIMNVL
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHHH
NPKVAMFFLAFLPQFVAPAAGRVPLQMLLLGLIFMVQSVVIFCLLGYFAGSIGSFILARP
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
RIARYFDWLTAGVFLSLGVRLALAER
HHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]