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The map label for this gene is mlaD [H]

Identifier: 78222006

GI number: 78222006

Start: 867761

End: 868213

Strand: Reverse

Name: mlaD [H]

Synonym: Gmet_0786

Alternate gene names: 78222006

Gene position: 868213-867761 (Counterclockwise)

Preceding gene: 78222007

Following gene: 78222005

Centisome position: 21.72

GC content: 60.26

Gene sequence:

>453_bases
ATGAAGAAAGTCACTCTCGAACTCATTGTCGGCATTTTCGTGGCCATCGGCATCCTCTGCCTCGGGTGGCTCTCGGTGAA
GCTGGGCAAGATGGAGCTGGTCGGCGGGGACCACTACGAGGTCTACGCCGAATTCGACTCCGTGTCGGGACTCAAGGAGG
GGGCAGCGGTGGAGATTGCCGGGGTGGAGGTCGGCCGGGTCGACAGGATCGCTCTCGATCCGAAGTTCAATGACCGGGCA
CGGGTCTCCATGCGGATCGCCAACGGGGTAAAGCTCCAGGACGATGTCATCGCCTCGGTACGCACCAGCGGCATCATCGG
CGACAAATTCATCCTGCTGAAGCCCGGCGGCTCCGATAACTTTCTGGCCGACAACGGCAGGATCAGGGAGACCGAATCGG
CAGTTGATCTGGAGGAACTGCTGAGCAAGTACATCCACGGCAAGGTGGAGTGA

Upstream 100 bases:

>100_bases
GGAGGAGATCCGGCGCTCGGAGCACCCGGTGGTGCGGCAGTTCATCAGCGGCAGTCTCGAAGGACCCATCAAGTTCATCT
GACACAGGCAGGGGATTGTT

Downstream 100 bases:

>100_bases
CTGAGCCGCATTCCAGGAGGGAAGGAAATGAGAACACTTCGCGCAGTGGCACTGGCATGTCTGACGGCACTCCCGCTATC
CGGGGCGCTGGCCGCCGAAC

Product: virulence factor MCE-like protein-related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 150; Mature: 150

Protein sequence:

>150_residues
MKKVTLELIVGIFVAIGILCLGWLSVKLGKMELVGGDHYEVYAEFDSVSGLKEGAAVEIAGVEVGRVDRIALDPKFNDRA
RVSMRIANGVKLQDDVIASVRTSGIIGDKFILLKPGGSDNFLADNGRIRETESAVDLEELLSKYIHGKVE

Sequences:

>Translated_150_residues
MKKVTLELIVGIFVAIGILCLGWLSVKLGKMELVGGDHYEVYAEFDSVSGLKEGAAVEIAGVEVGRVDRIALDPKFNDRA
RVSMRIANGVKLQDDVIASVRTSGIIGDKFILLKPGGSDNFLADNGRIRETESAVDLEELLSKYIHGKVE
>Mature_150_residues
MKKVTLELIVGIFVAIGILCLGWLSVKLGKMELVGGDHYEVYAEFDSVSGLKEGAAVEIAGVEVGRVDRIALDPKFNDRA
RVSMRIANGVKLQDDVIASVRTSGIIGDKFILLKPGGSDNFLADNGRIRETESAVDLEELLSKYIHGKVE

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG1463

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, periplasmic component

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein; Periplasmic side (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaD family [H]

Homologues:

Organism=Escherichia coli, GI1789584, Length=154, Percent_Identity=33.7662337662338, Blast_Score=74, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003399 [H]

Pfam domain/function: PF02470 MCE [H]

EC number: NA

Molecular weight: Translated: 16260; Mature: 16260

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVTLELIVGIFVAIGILCLGWLSVKLGKMELVGGDHYEVYAEFDSVSGLKEGAAVEIA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEE
GVEVGRVDRIALDPKFNDRARVSMRIANGVKLQDDVIASVRTSGIIGDKFILLKPGGSDN
CCEECCEEEEEECCCCCCCEEEEEEECCCCEECHHHHHHHHHCCCCCCEEEEEECCCCCC
FLADNGRIRETESAVDLEELLSKYIHGKVE
EEECCCCEECCCHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MKKVTLELIVGIFVAIGILCLGWLSVKLGKMELVGGDHYEVYAEFDSVSGLKEGAAVEIA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEE
GVEVGRVDRIALDPKFNDRARVSMRIANGVKLQDDVIASVRTSGIIGDKFILLKPGGSDN
CCEECCEEEEEECCCCCCCEEEEEEECCCCEECHHHHHHHHHCCCCCCEEEEEECCCCCC
FLADNGRIRETESAVDLEELLSKYIHGKVE
EEECCCCEECCCHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]