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The map label for this gene is cheR [C]

Identifier: 78222000

GI number: 78222000

Start: 858077

End: 860740

Strand: Reverse

Name: cheR [C]

Synonym: Gmet_0780

Alternate gene names: 78222000

Gene position: 860740-858077 (Counterclockwise)

Preceding gene: 78222003

Following gene: 78221999

Centisome position: 21.53

GC content: 58.75

Gene sequence:

>2664_bases
ATGACAAAAAAGTCCACCAAGACCTCCGTTGCGGGGAAAAAGCCCCCCACCCCCGCCCCTTCAGCTCCCGAACCTGAGCG
GAAAAGTGATGAAACGCATTTCCCCATCGTCGCCATCGGGGCGTCCGCCGGGGGGCTGGAGGCCCTGACACAGTTCCTTG
AGCACGTCCCCCAGAACAGCGGCCTGGCCTTTGTGGTGATCCAGCATCTCGACCCGGACCATATCGCGCTCCTGCCCGAA
CTGCTGCAGCGGGTCACCAGCATGAAGGTAACGGCGGTCAAGGACCGGAGTCGAGTCAGGCCTGACTCCGTCTACGTCAT
CCCCCCCAATCGCGACATGTCGATCCTCCACGGGGTGCTGCACCTGTTCGAGGCCTCCGAACCCCGGGGCCAGCGCCTCC
CCATCGACCACTTCTTCCGCTCCCTTGCCGAAGACTGCAAGGAGCGGAGCATCGGCGTAATCCTTTCCGGCATGGGATCG
GACGGCACCTTAGGGCTGAGGGCCATCAAGGAGAAGGGGGGGCTCACCCTGGTGCAGGACCCCGACTCGGCCAAGTTCAG
CGGCATGCCCAACAGCGCCATCAGTTTCGGTCTTGCTGACATAGTTGCCCGGGCCGAGAATATCCCCGGGAAAATCATGG
ACTTTCTCCAGCACACCCCCGCACCTTCGCAGCAGAAGACGAAACTGGAGGACAAGGAGCTGAGTGCCCTCGACAAGATC
GTCATCCTGCTCCGGAGCGAGACGGGGAACGATTTCTCCCTTTACAAGAAGAGTACCCTCTACCGCCGGATAGAGCGGCG
AATGGGGATTCACCAGATAGATTCCATCGCCGGCTACGTGCGCTACATCCAGGCGAATCACCAGGAACTGGAACTGCTCT
TCAAGGAGTTCCTCATTGGCGTGACCAGTTTTTTCAGGGACCCTGCCGCGTGGGAAAACCTAAAAAACGAGGTAATTCCG
GATTTGCTGGCCAGCCACCCGGAGGGGGGGGTACTGCGGGCATGGTCGGCCGGGTGCTCCACCGGCGAAGAGGCCTATTC
CCTGGCCATCATCTTCAGGGAAGTCCTGGAGCAGGTCAAACCCAAGGCCCATTTCAGCCTGCAAGTCTTTGCCACCGATC
TCGACCGGGACGCCATAGACAAGGCCCGGCTGGGGCTCTTTCTCCCCAACATTGCCAGTGATGTTTCCGCCGAGCGGCTG
AACCGTTATTTCGTCAAGGATGAAGACGGGTACCGGATCAAGAAGGAAATCCGTGAAATGGTCACGTTCGCCACCCAGAA
CGTTATCACCGATCCCCCCTTCACCAAGCTCGACATTCTCATCTGCCGCAATCTGCTGATCTATCTGGACGTTGAAATGC
AAAAGAAACTCCTCCCCCTCTTCCACTACGTTCTCAACCCGGGAGGGGTGCTGTTTCTCGGCAGTGCCGAGACCCTCGGC
GCCAGTGCCGAGCTTTTCACGGCCGTCAATGCCAAATTGAGGCTTTTCAGACGGGACAACGGCGTCAGCCGGGCGAGTCT
GGCGTCGTTCCCCTCAACGTTCCCCCTTGCGACAGCCTCCCACAAGGAGCTCTCGCCCGTGAAACCCGAGATCAACTTCC
AGGTCCTGGCCGACCGAATCCTGCTGCAAAAGCACTCCCCCCCCGCTGTTCTCGTGAACGAGGAAGGGGACATCGTCTAC
ATCAGCGCCCGGATCGGTAAATATCTCGAGCCGCCGGTGGGGAAAGCGAACATTAACGTTTTCGCCATGGCCCGGGAGGG
GTTACGCCCCGAACTGCTCGCCGCATTCCACAAGGCAATGCGGCAGAATGCGCCGGTTATCACGCGGAACATCCGGATCA
GCGGCAACGGGCATCCCCAAACCCTCGACCTTATTGTCCAGAAGATGGAAGAGCCGGAACTGCAGGGGATGGCCATGATC
ATTTTCAAGGATCTGGAGCCCTGTCAGGAATCTCCGGTCAAGAAACGCGGCGTCAAGTCATCGGATGCCGTAACCGCCCG
GATTGGCGAACTGGAACTGGAACTGCAGCAGACCCAGGAGAAGCTCCGGATTACCCTTGAGGAATTGCAGACCACGCGGG
AGGAGATGCAAACCTCACAGGAAGAGTTGAAAGCCTCGAATGAGGAGTTACAATCTATAAACGAGGAGTTGCAGTCCACC
AACGAAGAGCTGACCACCTCCAAGGAGGAGATGCAGTCCATGAACGAGGAACTGCAGACGGTGAACTCCGAGCAGCAGGC
CAAGGTGGATGAACTGTCGCACCTGAACGACGACATGCGGAACTTCCTCAACAGCACCGAGGTGGCCACCATCTTCCTGG
ACAGCAAGCTGAACATCCGCCGCTTCGCCAACGCCTCCAGCAAGCTGTTCAAGCTGATCCCAAGCGATGTGGGGCGCCCC
CTCACCGACATCGTCAACGATCTCAGCTACCCTGAACTGACCGACGATGCCGAGGCAGTGCTCCGGACGCTGGTCTATTC
GGAAAAGCAGGTGACCACCAACGACGGACGTTGGTTCTCGGTTCGGATCATGCCGTACCGCACGGTGAGTGATGTGATCG
ACGGGGTGGTAATCACCATGACCGACATCACGCCATTCAAGAAGCTGGAAGAGGAACTCAGGGATGAGGTCAAAAAACTG
CTGCAGGGGCGGAAAGAAGAATGA

Upstream 100 bases:

>100_bases
CGACAGGGATTTGCTATCGCTATCCACACCTGTTATCTTGCCAATCACCACTGCTGCGGATATTATTTTTAACTCTAACG
AAACGTTACGGAGACCATAG

Downstream 100 bases:

>100_bases
TGCCCCACCTGGCGCGCCGCAGGACGTAACGCTCCGCCGCGGCGCGGAGAGACGCCTCATGGAGCTTCCCCTTCAAAGCG
TTGTCCCCAGGACGGAGGGG

Product: MCP methyltransferase/methylesterase, CheR/CheB with PAS/PAC sensor

Products: S-adenosyl-L-homocysteine; protein L-glutamate methyl ester; Tsrglu-Me; Trgglu-Me; Targlu-Me; Tapglu-Me + [C]

Alternate protein names: ORF19 [H]

Number of amino acids: Translated: 887; Mature: 886

Protein sequence:

>887_residues
MTKKSTKTSVAGKKPPTPAPSAPEPERKSDETHFPIVAIGASAGGLEALTQFLEHVPQNSGLAFVVIQHLDPDHIALLPE
LLQRVTSMKVTAVKDRSRVRPDSVYVIPPNRDMSILHGVLHLFEASEPRGQRLPIDHFFRSLAEDCKERSIGVILSGMGS
DGTLGLRAIKEKGGLTLVQDPDSAKFSGMPNSAISFGLADIVARAENIPGKIMDFLQHTPAPSQQKTKLEDKELSALDKI
VILLRSETGNDFSLYKKSTLYRRIERRMGIHQIDSIAGYVRYIQANHQELELLFKEFLIGVTSFFRDPAAWENLKNEVIP
DLLASHPEGGVLRAWSAGCSTGEEAYSLAIIFREVLEQVKPKAHFSLQVFATDLDRDAIDKARLGLFLPNIASDVSAERL
NRYFVKDEDGYRIKKEIREMVTFATQNVITDPPFTKLDILICRNLLIYLDVEMQKKLLPLFHYVLNPGGVLFLGSAETLG
ASAELFTAVNAKLRLFRRDNGVSRASLASFPSTFPLATASHKELSPVKPEINFQVLADRILLQKHSPPAVLVNEEGDIVY
ISARIGKYLEPPVGKANINVFAMAREGLRPELLAAFHKAMRQNAPVITRNIRISGNGHPQTLDLIVQKMEEPELQGMAMI
IFKDLEPCQESPVKKRGVKSSDAVTARIGELELELQQTQEKLRITLEELQTTREEMQTSQEELKASNEELQSINEELQST
NEELTTSKEEMQSMNEELQTVNSEQQAKVDELSHLNDDMRNFLNSTEVATIFLDSKLNIRRFANASSKLFKLIPSDVGRP
LTDIVNDLSYPELTDDAEAVLRTLVYSEKQVTTNDGRWFSVRIMPYRTVSDVIDGVVITMTDITPFKKLEEELRDEVKKL
LQGRKEE

Sequences:

>Translated_887_residues
MTKKSTKTSVAGKKPPTPAPSAPEPERKSDETHFPIVAIGASAGGLEALTQFLEHVPQNSGLAFVVIQHLDPDHIALLPE
LLQRVTSMKVTAVKDRSRVRPDSVYVIPPNRDMSILHGVLHLFEASEPRGQRLPIDHFFRSLAEDCKERSIGVILSGMGS
DGTLGLRAIKEKGGLTLVQDPDSAKFSGMPNSAISFGLADIVARAENIPGKIMDFLQHTPAPSQQKTKLEDKELSALDKI
VILLRSETGNDFSLYKKSTLYRRIERRMGIHQIDSIAGYVRYIQANHQELELLFKEFLIGVTSFFRDPAAWENLKNEVIP
DLLASHPEGGVLRAWSAGCSTGEEAYSLAIIFREVLEQVKPKAHFSLQVFATDLDRDAIDKARLGLFLPNIASDVSAERL
NRYFVKDEDGYRIKKEIREMVTFATQNVITDPPFTKLDILICRNLLIYLDVEMQKKLLPLFHYVLNPGGVLFLGSAETLG
ASAELFTAVNAKLRLFRRDNGVSRASLASFPSTFPLATASHKELSPVKPEINFQVLADRILLQKHSPPAVLVNEEGDIVY
ISARIGKYLEPPVGKANINVFAMAREGLRPELLAAFHKAMRQNAPVITRNIRISGNGHPQTLDLIVQKMEEPELQGMAMI
IFKDLEPCQESPVKKRGVKSSDAVTARIGELELELQQTQEKLRITLEELQTTREEMQTSQEELKASNEELQSINEELQST
NEELTTSKEEMQSMNEELQTVNSEQQAKVDELSHLNDDMRNFLNSTEVATIFLDSKLNIRRFANASSKLFKLIPSDVGRP
LTDIVNDLSYPELTDDAEAVLRTLVYSEKQVTTNDGRWFSVRIMPYRTVSDVIDGVVITMTDITPFKKLEEELRDEVKKL
LQGRKEE
>Mature_886_residues
TKKSTKTSVAGKKPPTPAPSAPEPERKSDETHFPIVAIGASAGGLEALTQFLEHVPQNSGLAFVVIQHLDPDHIALLPEL
LQRVTSMKVTAVKDRSRVRPDSVYVIPPNRDMSILHGVLHLFEASEPRGQRLPIDHFFRSLAEDCKERSIGVILSGMGSD
GTLGLRAIKEKGGLTLVQDPDSAKFSGMPNSAISFGLADIVARAENIPGKIMDFLQHTPAPSQQKTKLEDKELSALDKIV
ILLRSETGNDFSLYKKSTLYRRIERRMGIHQIDSIAGYVRYIQANHQELELLFKEFLIGVTSFFRDPAAWENLKNEVIPD
LLASHPEGGVLRAWSAGCSTGEEAYSLAIIFREVLEQVKPKAHFSLQVFATDLDRDAIDKARLGLFLPNIASDVSAERLN
RYFVKDEDGYRIKKEIREMVTFATQNVITDPPFTKLDILICRNLLIYLDVEMQKKLLPLFHYVLNPGGVLFLGSAETLGA
SAELFTAVNAKLRLFRRDNGVSRASLASFPSTFPLATASHKELSPVKPEINFQVLADRILLQKHSPPAVLVNEEGDIVYI
SARIGKYLEPPVGKANINVFAMAREGLRPELLAAFHKAMRQNAPVITRNIRISGNGHPQTLDLIVQKMEEPELQGMAMII
FKDLEPCQESPVKKRGVKSSDAVTARIGELELELQQTQEKLRITLEELQTTREEMQTSQEELKASNEELQSINEELQSTN
EELTTSKEEMQSMNEELQTVNSEQQAKVDELSHLNDDMRNFLNSTEVATIFLDSKLNIRRFANASSKLFKLIPSDVGRPL
TDIVNDLSYPELTDDAEAVLRTLVYSEKQVTTNDGRWFSVRIMPYRTVSDVIDGVVITMTDITPFKKLEEELRDEVKKLL
QGRKEE

Specific function: Methylation Of The Membrane-Bound Methyl-Accepting Chemotaxis Proteins (Mcp) To Form Gamma-Glutamyl Methyl Ester Residues In Mcp. [C]

COG id: COG1352

COG function: function code NT; Methylase of chemotaxis methyl-accepting proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 cheR-type methyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1788193, Length=262, Percent_Identity=29.0076335877863, Blast_Score=107, Evalue=4e-24,
Organism=Escherichia coli, GI1788192, Length=201, Percent_Identity=30.3482587064677, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022642
- InterPro:   IPR000780
- InterPro:   IPR022641
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR000673 [H]

Pfam domain/function: PF01339 CheB_methylest; PF01739 CheR; PF03705 CheR_N; PF08448 PAS_4 [H]

EC number: 2.1.1.80 [C]

Molecular weight: Translated: 99296; Mature: 99165

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS50113 PAC ; PS00092 N6_MTASE ; PS50123 CHER ; PS50122 CHEB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKSTKTSVAGKKPPTPAPSAPEPERKSDETHFPIVAIGASAGGLEALTQFLEHVPQNS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCC
GLAFVVIQHLDPDHIALLPELLQRVTSMKVTAVKDRSRVRPDSVYVIPPNRDMSILHGVL
CEEEEEEECCCCHHHHHHHHHHHHHHHCEEEEECHHCCCCCCEEEEECCCCCHHHHHHHH
HLFEASEPRGQRLPIDHFFRSLAEDCKERSIGVILSGMGSDGTLGLRAIKEKGGLTLVQD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEC
PDSAKFSGMPNSAISFGLADIVARAENIPGKIMDFLQHTPAPSQQKTKLEDKELSALDKI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHH
VILLRSETGNDFSLYKKSTLYRRIERRMGIHQIDSIAGYVRYIQANHQELELLFKEFLIG
EEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VTSFFRDPAAWENLKNEVIPDLLASHPEGGVLRAWSAGCSTGEEAYSLAIIFREVLEQVK
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
PKAHFSLQVFATDLDRDAIDKARLGLFLPNIASDVSAERLNRYFVKDEDGYRIKKEIREM
CCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHH
VTFATQNVITDPPFTKLDILICRNLLIYLDVEMQKKLLPLFHYVLNPGGVLFLGSAETLG
HHHHHHHCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCHHCC
ASAELFTAVNAKLRLFRRDNGVSRASLASFPSTFPLATASHKELSPVKPEINFQVLADRI
CCHHHHHHHHHHHEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LLQKHSPPAVLVNEEGDIVYISARIGKYLEPPVGKANINVFAMAREGLRPELLAAFHKAM
HHHCCCCCEEEEECCCCEEEEEHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
RQNAPVITRNIRISGNGHPQTLDLIVQKMEEPELQGMAMIIFKDLEPCQESPVKKRGVKS
HCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCHHHCCCCC
SDAVTARIGELELELQQTQEKLRITLEELQTTREEMQTSQEELKASNEELQSINEELQST
CCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEELTTSKEEMQSMNEELQTVNSEQQAKVDELSHLNDDMRNFLNSTEVATIFLDSKLNIR
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCHH
RFANASSKLFKLIPSDVGRPLTDIVNDLSYPELTDDAEAVLRTLVYSEKQVTTNDGRWFS
HHHCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEE
VRIMPYRTVSDVIDGVVITMTDITPFKKLEEELRDEVKKLLQGRKEE
EEEEECCHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TKKSTKTSVAGKKPPTPAPSAPEPERKSDETHFPIVAIGASAGGLEALTQFLEHVPQNS
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCC
GLAFVVIQHLDPDHIALLPELLQRVTSMKVTAVKDRSRVRPDSVYVIPPNRDMSILHGVL
CEEEEEEECCCCHHHHHHHHHHHHHHHCEEEEECHHCCCCCCEEEEECCCCCHHHHHHHH
HLFEASEPRGQRLPIDHFFRSLAEDCKERSIGVILSGMGSDGTLGLRAIKEKGGLTLVQD
HHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHCCCEEEEEC
PDSAKFSGMPNSAISFGLADIVARAENIPGKIMDFLQHTPAPSQQKTKLEDKELSALDKI
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHCCCHHHHHHHHHH
VILLRSETGNDFSLYKKSTLYRRIERRMGIHQIDSIAGYVRYIQANHQELELLFKEFLIG
EEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VTSFFRDPAAWENLKNEVIPDLLASHPEGGVLRAWSAGCSTGEEAYSLAIIFREVLEQVK
HHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHCC
PKAHFSLQVFATDLDRDAIDKARLGLFLPNIASDVSAERLNRYFVKDEDGYRIKKEIREM
CCCCEEEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHH
VTFATQNVITDPPFTKLDILICRNLLIYLDVEMQKKLLPLFHYVLNPGGVLFLGSAETLG
HHHHHHHCCCCCCCHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCEEEEECCHHCC
ASAELFTAVNAKLRLFRRDNGVSRASLASFPSTFPLATASHKELSPVKPEINFQVLADRI
CCHHHHHHHHHHHEEEECCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LLQKHSPPAVLVNEEGDIVYISARIGKYLEPPVGKANINVFAMAREGLRPELLAAFHKAM
HHHCCCCCEEEEECCCCEEEEEHHCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHH
RQNAPVITRNIRISGNGHPQTLDLIVQKMEEPELQGMAMIIFKDLEPCQESPVKKRGVKS
HCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCHHHCCCHHHCCCCC
SDAVTARIGELELELQQTQEKLRITLEELQTTREEMQTSQEELKASNEELQSINEELQST
CCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NEELTTSKEEMQSMNEELQTVNSEQQAKVDELSHLNDDMRNFLNSTEVATIFLDSKLNIR
HHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCCHH
RFANASSKLFKLIPSDVGRPLTDIVNDLSYPELTDDAEAVLRTLVYSEKQVTTNDGRWFS
HHHCHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCEEE
VRIMPYRTVSDVIDGVVITMTDITPFKKLEEELRDEVKKLLQGRKEE
EEEEECCHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Ca2+; Mg2+ [C]

Kcat value (1/min): 10 [C]

Specific activity: NA

Km value (mM): 0.017 {S-adenosyl-L-methionine}} 0.01 {S-adenosyl-L-methionine}} [C]

Substrates: S-Adenosyl-L-methionine; protein L-glutamate; Tsrglu; Trgglu; Targlu; Tapglu [C]

Specific reaction: S-Adenosyl-L-methionine + protein L-glutamate =S-adenosyl-L-homocysteine + protein L-glutamate methyl ester S-adenosyl-L-methionine + Tsrglu = S-adenosyl-homocysteine + Tsrglu-Me S-adenosyl-L-methionine + Trgglu = S-adenosyl-homocysteine + Trgglu-Me S-ade

General reaction: Methyl group transfer [C]

Inhibitor: A9145C; Ca2+; Methylated receptor protein; S-Adenosyl -L-homocysteine; Sinefungin [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497190 [H]