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The map label for this gene is oprF [H]

Identifier: 78221995

GI number: 78221995

Start: 850874

End: 852247

Strand: Direct

Name: oprF [H]

Synonym: Gmet_0775

Alternate gene names: 78221995

Gene position: 850874-852247 (Clockwise)

Preceding gene: 78221987

Following gene: 78222001

Centisome position: 21.29

GC content: 60.19

Gene sequence:

>1374_bases
ATGAAGAGACTATTGGCTGCAGGATGCGCACTTTTCGCCCTTGCCTTTGCCACGACGGCGATGGCGGAGGTGAAGCCGGG
TTCATTTGCCATCACTCCCTACGTGGGAGGATACACCTTCGATGGCGAGCAGCACATCAAGACGGGCCCCACCTTCGGTA
TCCGTGGTGGGTACAATTTTACGAAAAACTGTGGGCTTGAAGGGGTCTTCGGCTATACCCCCACCGAGCAGACAAAGCGC
GAGTCCGATGTTGATGCCATCACCTACCGTCTTGAGGGGCTCTATCACTTCATGCCGGAAAAGGATCTGGTGCCGTTTCT
GGCCGTTGGCGGCGGCGGAATCACCATCGACAGTGAGGGCGCCGGGGTGAAGAGCCGTACCGACGGCATCGTCACGGCCG
GTGGTGGCCTCAAGTATTTCATCAACGAGGCGCTTGCGGTGCGGACCGATGGCCGCTACATCCTCGACCTGAGCCGGACG
CTCAGCAACTGGGAATACACCGTCGGCCTCAGTTTCCTGTTTGGCGGCGAGAAGAAGGCTGTCGCTCCCGTAGCCCCCGC
GGCTGCTCCTGCTGAAGCGACCCCGGCACCCGAGGCCCCTCCCGCAGTCATGGCTGCCGAGCCGCGCCCTGGCCTCATGA
AGTACTGCACCGATCTCAAGATCGAGTTCGACATCGACAAGGCCGACATCCGGCCCCAGTACAAGGACGAGGTGAAGCGT
GTCGCCGACTACATGAAGCAGTATCCGAACACCACGGCGGTCATCGAAGGGTATACCGACGACGTGGGGAGCGACGACTA
CAACATGAAGCTCTCCCAGCGGCGGGCCGAAGCCGTTGTGAATTCCCTCGTGGATGATTTCGGGATTGACCGCTCACGCC
TCTCCGCCAAGGGGTATGGGAAGACCCAGCCCCTTACCTCCAACGCCACCGAGGCGGGAAAACAGAAGAACCGCAGGATC
CAGGCGGTCATCGACTGCACCCTGGAGGATCCCAAGGTTATCGCCAAGCTTCCGGACCGGCTCTGTGTGAGCCTCAATAT
CCAGTTCGACACCGACAAGTACACCATCAAGCCCCAGTACCATGACGAAATAGCCAAGGTGGGCGAGTTCATGAAGCAAA
ATCCCACGACAACCGCAGTTATCGAAGGGCACACCGACCGCGTGGGGAGCTACGACTACAACATGAAGCTCTCCCTGCGC
CGCGCCGAGAGTGTCGTGACCTATCTGGCCGACAAGTTCGGCATCGAGAGATCCCGCCTCTCCGCCAAGGGGTACGGCTA
TACCCGCCCCATCGGCTACAACTCCGACCCGAAGGGACGCGCCATGAACCGGCGGATCAACGCCATCATCGACTGCGTGT
TGCAGCAGAAGTAA

Upstream 100 bases:

>100_bases
AGCTTCGCGCATTTGCATCCGTTGACAAGCGGTAATGCCGGTCTATAGTTTTTCCGAGATTGACTTGAGGCTGCCTTACA
CAGAACAAGGAGGTTCCGCG

Downstream 100 bases:

>100_bases
GAAATAAACTCTCTTCTTCAAGAGAAAAGGCCGTCTCCCTAGTGGGGGACGGCCTTTTCTCTTGGTGGGATACCCAAAGC
TCATTTCCTGGCGTCGATCC

Product: OmpA/MotB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MKRLLAAGCALFALAFATTAMAEVKPGSFAITPYVGGYTFDGEQHIKTGPTFGIRGGYNFTKNCGLEGVFGYTPTEQTKR
ESDVDAITYRLEGLYHFMPEKDLVPFLAVGGGGITIDSEGAGVKSRTDGIVTAGGGLKYFINEALAVRTDGRYILDLSRT
LSNWEYTVGLSFLFGGEKKAVAPVAPAAAPAEATPAPEAPPAVMAAEPRPGLMKYCTDLKIEFDIDKADIRPQYKDEVKR
VADYMKQYPNTTAVIEGYTDDVGSDDYNMKLSQRRAEAVVNSLVDDFGIDRSRLSAKGYGKTQPLTSNATEAGKQKNRRI
QAVIDCTLEDPKVIAKLPDRLCVSLNIQFDTDKYTIKPQYHDEIAKVGEFMKQNPTTTAVIEGHTDRVGSYDYNMKLSLR
RAESVVTYLADKFGIERSRLSAKGYGYTRPIGYNSDPKGRAMNRRINAIIDCVLQQK

Sequences:

>Translated_457_residues
MKRLLAAGCALFALAFATTAMAEVKPGSFAITPYVGGYTFDGEQHIKTGPTFGIRGGYNFTKNCGLEGVFGYTPTEQTKR
ESDVDAITYRLEGLYHFMPEKDLVPFLAVGGGGITIDSEGAGVKSRTDGIVTAGGGLKYFINEALAVRTDGRYILDLSRT
LSNWEYTVGLSFLFGGEKKAVAPVAPAAAPAEATPAPEAPPAVMAAEPRPGLMKYCTDLKIEFDIDKADIRPQYKDEVKR
VADYMKQYPNTTAVIEGYTDDVGSDDYNMKLSQRRAEAVVNSLVDDFGIDRSRLSAKGYGKTQPLTSNATEAGKQKNRRI
QAVIDCTLEDPKVIAKLPDRLCVSLNIQFDTDKYTIKPQYHDEIAKVGEFMKQNPTTTAVIEGHTDRVGSYDYNMKLSLR
RAESVVTYLADKFGIERSRLSAKGYGYTRPIGYNSDPKGRAMNRRINAIIDCVLQQK
>Mature_457_residues
MKRLLAAGCALFALAFATTAMAEVKPGSFAITPYVGGYTFDGEQHIKTGPTFGIRGGYNFTKNCGLEGVFGYTPTEQTKR
ESDVDAITYRLEGLYHFMPEKDLVPFLAVGGGGITIDSEGAGVKSRTDGIVTAGGGLKYFINEALAVRTDGRYILDLSRT
LSNWEYTVGLSFLFGGEKKAVAPVAPAAAPAEATPAPEAPPAVMAAEPRPGLMKYCTDLKIEFDIDKADIRPQYKDEVKR
VADYMKQYPNTTAVIEGYTDDVGSDDYNMKLSQRRAEAVVNSLVDDFGIDRSRLSAKGYGKTQPLTSNATEAGKQKNRRI
QAVIDCTLEDPKVIAKLPDRLCVSLNIQFDTDKYTIKPQYHDEIAKVGEFMKQNPTTTAVIEGHTDRVGSYDYNMKLSLR
RAESVVTYLADKFGIERSRLSAKGYGYTRPIGYNSDPKGRAMNRRINAIIDCVLQQK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI48994946, Length=106, Percent_Identity=37.7358490566038, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1787191, Length=355, Percent_Identity=24.5070422535211, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788957, Length=110, Percent_Identity=35.4545454545455, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786962, Length=99, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 50138; Mature: 50138

Theoretical pI: Translated: 8.06; Mature: 8.06

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLLAAGCALFALAFATTAMAEVKPGSFAITPYVGGYTFDGEQHIKTGPTFGIRGGYNF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCEEECCCCCC
TKNCGLEGVFGYTPTEQTKRESDVDAITYRLEGLYHFMPEKDLVPFLAVGGGGITIDSEG
CCCCCCCEECCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEECCCC
AGVKSRTDGIVTAGGGLKYFINEALAVRTDGRYILDLSRTLSNWEYTVGLSFLFGGEKKA
CCCCCCCCCEEECCCCHHHHHCCEEEEECCCCEEEEEHHHHCCCEEEEEEEEEECCCCCC
VAPVAPAAAPAEATPAPEAPPAVMAAEPRPGLMKYCTDLKIEFDIDKADIRPQYKDEVKR
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCEEEEECCHHCCCCCHHHHHHH
VADYMKQYPNTTAVIEGYTDDVGSDDYNMKLSQRRAEAVVNSLVDDFGIDRSRLSAKGYG
HHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
KTQPLTSNATEAGKQKNRRIQAVIDCTLEDPKVIAKLPDRLCVSLNIQFDTDKYTIKPQY
CCCCCCCCHHHHHHHHCCEEEEEEEECCCCCHHHHHCCCEEEEEEEEEECCCCEEECCCH
HDEIAKVGEFMKQNPTTTAVIEGHTDRVGSYDYNMKLSLRRAESVVTYLADKFGIERSRL
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHH
SAKGYGYTRPIGYNSDPKGRAMNRRINAIIDCVLQQK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKRLLAAGCALFALAFATTAMAEVKPGSFAITPYVGGYTFDGEQHIKTGPTFGIRGGYNF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCEEECCCCCCCCCCCEEECCCCCC
TKNCGLEGVFGYTPTEQTKRESDVDAITYRLEGLYHFMPEKDLVPFLAVGGGGITIDSEG
CCCCCCCEECCCCCCHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEECCCC
AGVKSRTDGIVTAGGGLKYFINEALAVRTDGRYILDLSRTLSNWEYTVGLSFLFGGEKKA
CCCCCCCCCEEECCCCHHHHHCCEEEEECCCCEEEEEHHHHCCCEEEEEEEEEECCCCCC
VAPVAPAAAPAEATPAPEAPPAVMAAEPRPGLMKYCTDLKIEFDIDKADIRPQYKDEVKR
CCCCCCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCEEEEECCHHCCCCCHHHHHHH
VADYMKQYPNTTAVIEGYTDDVGSDDYNMKLSQRRAEAVVNSLVDDFGIDRSRLSAKGYG
HHHHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCC
KTQPLTSNATEAGKQKNRRIQAVIDCTLEDPKVIAKLPDRLCVSLNIQFDTDKYTIKPQY
CCCCCCCCHHHHHHHHCCEEEEEEEECCCCCHHHHHCCCEEEEEEEEEECCCCEEECCCH
HDEIAKVGEFMKQNPTTTAVIEGHTDRVGSYDYNMKLSLRRAESVVTYLADKFGIERSRL
HHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHCCCHHHH
SAKGYGYTRPIGYNSDPKGRAMNRRINAIIDCVLQQK
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]