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The map label for this gene is leuB

Identifier: 78221823

GI number: 78221823

Start: 672920

End: 674008

Strand: Direct

Name: leuB

Synonym: Gmet_0602

Alternate gene names: 78221823

Gene position: 672920-674008 (Clockwise)

Preceding gene: 78221822

Following gene: 78221824

Centisome position: 16.83

GC content: 62.72

Gene sequence:

>1089_bases
ATGTCTCAGGTGTTCAAGGTGGCGGTTCTTCCCGGTGACGGCATCGGTCCCGAGGTTATGGCCGAGGCGCTCCGGGTGCT
GGACGCCATAGAGAAGAAGTACGACGTGAAATTCGAGCGGACCCACGCCAATGTCGGGGGCGCCGGTATTGACCTTGAAG
GGAAGGCCCTCCCCGAAACTACCATTACTATCTGCAAGACGTCCGATGCCATCCTCTTCGGCTCCGTGGGCGGCCCCAAG
TGGGAGACGCTTCCCCCGGATGAGCAGCCCGAGCGGGGCGCGCTCCTTCCCCTCAGAAAGATCTTCGGTCTCTACGCCAA
CCTGCGGCCGGCCATCATTTTCCCGTCCCTGACGGGCGCTTCTTCCCTCAAGGAGGAGGTCATCGCCGGCGGTTTCAACG
TGCTGGTTATCCGCGAGCTGACCGGCGGCATCTACTTTGCCCAGCCCAAGGGGATCGAAGGCGAGGGGCGCGACCGGGTC
GGCTTCGACACCATGCGCTACAGTGTCCCCGAGATCGAACGGATCACCCACGTTGCCTTCCAGGCGGCTCGCAAGCGGGG
CAAAAAGGTCTGCTCCATCGACAAGGCCAACGTTCTCTCCTCGTCGGTCCTCTGGCGCGAGGTGGTCACCGGCATTGCCA
AGGAATACCCGGACGTGGAGCTCTCCCACATGTACGTGGACAATGCTGCCATGCAGCTGGTCCGCTGGCCCAAGCAGTTC
GATGTCATCCTCTGCGAGAACATGTTCGGCGATATCCTCTCCGATGAGGCAGCCATGCTGACCGGCTCCCTGGGGATGCT
CCCCTCCGCGTCGCTTGCCGAGGGGACCTTCGGCATGTACGAGCCCTCCGGCGGCAGCGCCCCGGACATTGCGGGGCAAG
GGATTGCGAACCCCATCGCCCAGATCCTCTCCATGGGGATGATGCTCCGCTTCTCCTTCGGCATGGTGGACGCGGCCGAT
GCCATTGACAACGCCGTGGCCAAGGTGCTCGACCAGGGATGTCGGACCCGTGACATCTTTCAGGAGAAGCCGGGCGAAAA
GCTCGTCAACACCAAAGAGATGGGTGACGCGATCATCGTGGCCCTGTAA

Upstream 100 bases:

>100_bases
CGTTTGGGCTGATTTCACTTTGCCGGTTTTTTGTTGACAACAGAGACTTTGCTCAATAGCATTAAGTACTTTTGTATATT
TCTTGTGCGGGGGTGTAGGG

Downstream 100 bases:

>100_bases
CGGATTTACCTTATTCCCGAAAAAGGAGTTCTGCCCATGAAAGTAGGAATCGTCGGTTGGCGCGGTATGGTCGGTTCGGT
TCTCCTCCAGCGGATGGTGG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSQVFKVAVLPGDGIGPEVMAEALRVLDAIEKKYDVKFERTHANVGGAGIDLEGKALPETTITICKTSDAILFGSVGGPK
WETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRV
GFDTMRYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIAQILSMGMMLRFSFGMVDAAD
AIDNAVAKVLDQGCRTRDIFQEKPGEKLVNTKEMGDAIIVAL

Sequences:

>Translated_362_residues
MSQVFKVAVLPGDGIGPEVMAEALRVLDAIEKKYDVKFERTHANVGGAGIDLEGKALPETTITICKTSDAILFGSVGGPK
WETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRV
GFDTMRYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQF
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIAQILSMGMMLRFSFGMVDAAD
AIDNAVAKVLDQGCRTRDIFQEKPGEKLVNTKEMGDAIIVAL
>Mature_361_residues
SQVFKVAVLPGDGIGPEVMAEALRVLDAIEKKYDVKFERTHANVGGAGIDLEGKALPETTITICKTSDAILFGSVGGPKW
ETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGASSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRVG
FDTMRYSVPEIERITHVAFQAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQFD
VILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIAQILSMGMMLRFSFGMVDAADA
IDNAVAKVLDQGCRTRDIFQEKPGEKLVNTKEMGDAIIVAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=340, Percent_Identity=33.5294117647059, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI4758582, Length=337, Percent_Identity=29.080118694362, Blast_Score=111, Evalue=9e-25,
Organism=Homo sapiens, GI28178838, Length=329, Percent_Identity=28.8753799392097, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28178821, Length=342, Percent_Identity=28.3625730994152, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI28178816, Length=313, Percent_Identity=29.3929712460064, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI28178819, Length=171, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI87081683, Length=358, Percent_Identity=60.6145251396648, Blast_Score=455, Evalue=1e-129,
Organism=Escherichia coli, GI1788101, Length=350, Percent_Identity=34.8571428571429, Blast_Score=174, Evalue=7e-45,
Organism=Escherichia coli, GI1787381, Length=400, Percent_Identity=26.5, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=332, Percent_Identity=32.8313253012048, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI25144293, Length=351, Percent_Identity=33.048433048433, Blast_Score=138, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17505779, Length=348, Percent_Identity=29.8850574712644, Blast_Score=120, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17550882, Length=356, Percent_Identity=30.0561797752809, Blast_Score=118, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=369, Percent_Identity=45.5284552845528, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6322097, Length=360, Percent_Identity=34.1666666666667, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6324291, Length=341, Percent_Identity=32.5513196480938, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6324709, Length=339, Percent_Identity=32.4483775811209, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24643268, Length=333, Percent_Identity=34.8348348348348, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24643270, Length=333, Percent_Identity=34.8348348348348, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24661184, Length=345, Percent_Identity=30.4347826086957, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078637, Length=341, Percent_Identity=29.0322580645161, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI161078635, Length=341, Percent_Identity=29.0322580645161, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI161078633, Length=341, Percent_Identity=29.0322580645161, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24650122, Length=341, Percent_Identity=29.0322580645161, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI161078639, Length=339, Percent_Identity=28.9085545722714, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI281362242, Length=336, Percent_Identity=27.3809523809524, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648872, Length=336, Percent_Identity=27.3809523809524, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_GEOMG (Q39Y29)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383570.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q39Y29
- SMR:   Q39Y29
- STRING:   Q39Y29
- GeneID:   3738949
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0602
- NMPDR:   fig|269799.3.peg.630
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   MSYQIAV
- PhylomeDB:   Q39Y29
- ProtClustDB:   PRK00772
- BioCyc:   GMET269799:GMET_0602-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39001; Mature: 38869

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 99-99 BINDING 109-109 BINDING 138-138 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVFKVAVLPGDGIGPEVMAEALRVLDAIEKKYDVKFERTHANVGGAGIDLEGKALPET
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCC
TITICKTSDAILFGSVGGPKWETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGA
EEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRVGFDTMRYSVPEIERITHVAF
HHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQF
HHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSMGMMLRFSFGMVDAADAIDNAVAKVLDQGCRTRDIFQEKPGEKLVNTKEMGDAIIV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCCHHHCCCEEEE
AL
EC
>Mature Secondary Structure 
SQVFKVAVLPGDGIGPEVMAEALRVLDAIEKKYDVKFERTHANVGGAGIDLEGKALPET
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCCC
TITICKTSDAILFGSVGGPKWETLPPDEQPERGALLPLRKIFGLYANLRPAIIFPSLTGA
EEEEEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCCCCH
SSLKEEVIAGGFNVLVIRELTGGIYFAQPKGIEGEGRDRVGFDTMRYSVPEIERITHVAF
HHHHHHHHHCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHH
QAARKRGKKVCSIDKANVLSSSVLWREVVTGIAKEYPDVELSHMYVDNAAMQLVRWPKQF
HHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHCCHHH
DVILCENMFGDILSDEAAMLTGSLGMLPSASLAEGTFGMYEPSGGSAPDIAGQGIANPIA
CEEEHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
QILSMGMMLRFSFGMVDAADAIDNAVAKVLDQGCRTRDIFQEKPGEKLVNTKEMGDAIIV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHCCHHHCCCEEEE
AL
EC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA