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The map label for this gene is irlS [H]

Identifier: 78221748

GI number: 78221748

Start: 565591

End: 567024

Strand: Reverse

Name: irlS [H]

Synonym: Gmet_0527

Alternate gene names: 78221748

Gene position: 567024-565591 (Counterclockwise)

Preceding gene: 78221749

Following gene: 78221747

Centisome position: 14.18

GC content: 64.09

Gene sequence:

>1434_bases
ATGTCCTCGACGGAGCGTGAAGTGACCCACCCGGGGGCTCTCTCCCTCACCGCACGGCTCACCATCCTCTCCACCCTGGC
CACCACGTGTGTTCTCCTCTTTGTCATCGTCTTCCAGTTTCTCGCCCTGGCAAGCGACCTGGAGTTCGAGGACAACAACT
TCATCATCGAGAAAATACGGGTGATCGAGGCGATCATTGCCCGCTACCCCGACGACTCCGCCCACCTTGATCAGGAAGTC
AACTGGGAAGGGGCCCTGCGGCAGGACACCCGTTACCTGGTTCGAATCATGGGGCGGCAGGGAAATGTGGTCATGGAGAC
ACGGGGAATGGACCGCATTGTTCCTGCCCGCCAGTTCCCCCCGCCAGCCCGGGATTCCCGCGCCATCGGCCAAGGCAAAA
AGCTCCACGTGCAAGACGGGCGGATCTATCTGCTGAACTCCGCCTGGGGAATGTCGGAGAAGGGGCTTTCATCCCGCTTT
ATCCAGGTGGCCCTCGACGTGACCGACGACGAGGATATCCTTCAGGGATACAAGCAGAAGATGGCTTTTGTGTTTCTGGC
CGGCATTTGCCTTTCGGCCGCTCTCAGTGCCGTGGTGGTGCGCCGGGGCCTCCGCCCCCTGGCAGAGATTACCGGTGCGG
CCGGACGCATCGATGTGACGACCCTTGATGAACGCCTCGGTGCCCGGGTATGGCCCCGGGAACTGGCCCGTTTCGCCGCC
GCCTTCGACGGCATGCTCGACCGCCTTGAAACGTCTTTCGACCGGCTCGAAGCATCCTCCGCCAACCTGGCCCACGAAAT
CCGCACCCCCATCAACATCCTTCGGGGCGAGGCCGAGGTGGCTATCTCCCGGGCCCGCTCCGCCGAAGAGTACCGGCGGG
TCATCGAGTCGAGCCTTGAGGAGTACGAGCGCCTCTCGCGCCTGATCGACAACATCCTCTTCCTGGCCAGGGCCGAGCAA
CGGATCGAGCCGATCCCCCTGGACATGCGCGGCGAACTGGAACTGCTCCTGGACTTCTACGGCACCCTGGCGGAAGAGAA
GGAGATAACCATTTCCTGCTCCGGCCGGGGTGAGATACATGCTGACCCGCTCCTCTTCCAGCGGGCCGTCGGCAACCTGC
TCTCCAACGCCATCCGCTACACCCCCACGGGAGGGGCGATCACCGTCTCCTTCACCCGGGACAACGACGGCGCCGCCCAT
CTCACCGTTACCGACACCGGCATCGGCATCGCGCCGGACGATCTCCTCCGGGTCTTCGACCGCTTCTACCGCAGCAGCGA
GGCCCGCGCCCTCAATGCCCAGGGGACCGGCCTCGGCCTCGCCATCGTCCGCTCCATCATGGACCTCCACGGGGGAAGCG
TTTCGGTCTCCAGCGAACCGGGGCGGGGAACCGCCGTGACGCTCCACTTTCCCGCAGCTCCGTCACCATTGTAA

Upstream 100 bases:

>100_bases
AGCGACACCAATATCGTCGACGTGGCAATCCGCCGCCTGCGGCGCAAGGTCGACGACCCCTTCGAGCGAAAGCTGATAAG
TACCGTCCGCGGAGCAGGCT

Downstream 100 bases:

>100_bases
CTTTTGAAGATGACGAAATTGTCATCTTCCCGTCATGGTCCGGTCAGGTTGTCTCTGGTAAAATTTTCCGCTGAAGTGCT
ATCTCCTGTCAGCGGAGCCC

Product: heavy metal sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MSSTEREVTHPGALSLTARLTILSTLATTCVLLFVIVFQFLALASDLEFEDNNFIIEKIRVIEAIIARYPDDSAHLDQEV
NWEGALRQDTRYLVRIMGRQGNVVMETRGMDRIVPARQFPPPARDSRAIGQGKKLHVQDGRIYLLNSAWGMSEKGLSSRF
IQVALDVTDDEDILQGYKQKMAFVFLAGICLSAALSAVVVRRGLRPLAEITGAAGRIDVTTLDERLGARVWPRELARFAA
AFDGMLDRLETSFDRLEASSANLAHEIRTPINILRGEAEVAISRARSAEEYRRVIESSLEEYERLSRLIDNILFLARAEQ
RIEPIPLDMRGELELLLDFYGTLAEEKEITISCSGRGEIHADPLLFQRAVGNLLSNAIRYTPTGGAITVSFTRDNDGAAH
LTVTDTGIGIAPDDLLRVFDRFYRSSEARALNAQGTGLGLAIVRSIMDLHGGSVSVSSEPGRGTAVTLHFPAAPSPL

Sequences:

>Translated_477_residues
MSSTEREVTHPGALSLTARLTILSTLATTCVLLFVIVFQFLALASDLEFEDNNFIIEKIRVIEAIIARYPDDSAHLDQEV
NWEGALRQDTRYLVRIMGRQGNVVMETRGMDRIVPARQFPPPARDSRAIGQGKKLHVQDGRIYLLNSAWGMSEKGLSSRF
IQVALDVTDDEDILQGYKQKMAFVFLAGICLSAALSAVVVRRGLRPLAEITGAAGRIDVTTLDERLGARVWPRELARFAA
AFDGMLDRLETSFDRLEASSANLAHEIRTPINILRGEAEVAISRARSAEEYRRVIESSLEEYERLSRLIDNILFLARAEQ
RIEPIPLDMRGELELLLDFYGTLAEEKEITISCSGRGEIHADPLLFQRAVGNLLSNAIRYTPTGGAITVSFTRDNDGAAH
LTVTDTGIGIAPDDLLRVFDRFYRSSEARALNAQGTGLGLAIVRSIMDLHGGSVSVSSEPGRGTAVTLHFPAAPSPL
>Mature_476_residues
SSTEREVTHPGALSLTARLTILSTLATTCVLLFVIVFQFLALASDLEFEDNNFIIEKIRVIEAIIARYPDDSAHLDQEVN
WEGALRQDTRYLVRIMGRQGNVVMETRGMDRIVPARQFPPPARDSRAIGQGKKLHVQDGRIYLLNSAWGMSEKGLSSRFI
QVALDVTDDEDILQGYKQKMAFVFLAGICLSAALSAVVVRRGLRPLAEITGAAGRIDVTTLDERLGARVWPRELARFAAA
FDGMLDRLETSFDRLEASSANLAHEIRTPINILRGEAEVAISRARSAEEYRRVIESSLEEYERLSRLIDNILFLARAEQR
IEPIPLDMRGELELLLDFYGTLAEEKEITISCSGRGEIHADPLLFQRAVGNLLSNAIRYTPTGGAITVSFTRDNDGAAHL
TVTDTGIGIAPDDLLRVFDRFYRSSEARALNAQGTGLGLAIVRSIMDLHGGSVSVSSEPGRGTAVTLHFPAAPSPL

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=485, Percent_Identity=29.6907216494845, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI1788279, Length=288, Percent_Identity=35.4166666666667, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1790346, Length=275, Percent_Identity=30.5454545454545, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786600, Length=239, Percent_Identity=29.7071129707113, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1790436, Length=215, Percent_Identity=31.1627906976744, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI145693157, Length=218, Percent_Identity=30.7339449541284, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788713, Length=221, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790861, Length=241, Percent_Identity=32.7800829875519, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788393, Length=247, Percent_Identity=28.3400809716599, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1786912, Length=231, Percent_Identity=29.4372294372294, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789403, Length=297, Percent_Identity=28.2828282828283, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI48994928, Length=224, Percent_Identity=28.125, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1789149, Length=222, Percent_Identity=26.5765765765766, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788549, Length=221, Percent_Identity=28.5067873303167, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789808, Length=317, Percent_Identity=27.4447949526814, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1787894, Length=307, Percent_Identity=28.6644951140065, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87081816, Length=233, Percent_Identity=30.4721030042918, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790551, Length=335, Percent_Identity=25.3731343283582, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 52549; Mature: 52417

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN ; PS01088 CAP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTEREVTHPGALSLTARLTILSTLATTCVLLFVIVFQFLALASDLEFEDNNFIIEKIR
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
VIEAIIARYPDDSAHLDQEVNWEGALRQDTRYLVRIMGRQGNVVMETRGMDRIVPARQFP
HHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
PPARDSRAIGQGKKLHVQDGRIYLLNSAWGMSEKGLSSRFIQVALDVTDDEDILQGYKQK
CCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHH
MAFVFLAGICLSAALSAVVVRRGLRPLAEITGAAGRIDVTTLDERLGARVWPRELARFAA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHH
AFDGMLDRLETSFDRLEASSANLAHEIRTPINILRGEAEVAISRARSAEEYRRVIESSLE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHH
EYERLSRLIDNILFLARAEQRIEPIPLDMRGELELLLDFYGTLAEEKEITISCSGRGEIH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
ADPLLFQRAVGNLLSNAIRYTPTGGAITVSFTRDNDGAAHLTVTDTGIGIAPDDLLRVFD
CCHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHH
RFYRSSEARALNAQGTGLGLAIVRSIMDLHGGSVSVSSEPGRGTAVTLHFPAAPSPL
HHHCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure 
SSTEREVTHPGALSLTARLTILSTLATTCVLLFVIVFQFLALASDLEFEDNNFIIEKIR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
VIEAIIARYPDDSAHLDQEVNWEGALRQDTRYLVRIMGRQGNVVMETRGMDRIVPARQFP
HHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCC
PPARDSRAIGQGKKLHVQDGRIYLLNSAWGMSEKGLSSRFIQVALDVTDDEDILQGYKQK
CCCCCCCCCCCCCEEEEECCEEEEEECCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHH
MAFVFLAGICLSAALSAVVVRRGLRPLAEITGAAGRIDVTTLDERLGARVWPRELARFAA
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEEHHHHHCCCCCHHHHHHHHH
AFDGMLDRLETSFDRLEASSANLAHEIRTPINILRGEAEVAISRARSAEEYRRVIESSLE
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHHH
EYERLSRLIDNILFLARAEQRIEPIPLDMRGELELLLDFYGTLAEEKEITISCSGRGEIH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCC
ADPLLFQRAVGNLLSNAIRYTPTGGAITVSFTRDNDGAAHLTVTDTGIGIAPDDLLRVFD
CCHHHHHHHHHHHHHHHEEECCCCCEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHH
RFYRSSEARALNAQGTGLGLAIVRSIMDLHGGSVSVSSEPGRGTAVTLHFPAAPSPL
HHHCCCCCCEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]