Click here to switch to the map view.

The map label for this gene is thiC1

Identifier: 78221692

GI number: 78221692

Start: 518299

End: 519606

Strand: Direct

Name: thiC1

Synonym: Gmet_0471

Alternate gene names: 78221692

Gene position: 518299-519606 (Clockwise)

Preceding gene: 78221691

Following gene: 161723103

Centisome position: 12.97

GC content: 65.6

Gene sequence:

>1308_bases
ATGAAAACCCAGATCGAAATCGCCCGCGAAGGGACCATTTCTTCCCAGATGATGGCCGTGGCAGCCGAAGAGCACGTTTC
CCCCGACTATGTCCGGCAGATGGTCGCCGAAGGGAAGATCGTCATCCCCGGCAACCACAGCCGCAAGCCCCGGGCCGTCG
GCATCGGCAAGGGACTGCGCACCAAGGTGAACGCCTCCATCGGTACCTCCTCCGACATCATCGACTATGGGGCCGAGGTG
CGGAAGGCCCTGGCCGCCCAGGAGGCGGGGGCCGACACCCTCATGGAGCTCTCCGTGGGGGGCGATCTGGACCGGGTGCG
GCGGGAGGTGATCGCCGCCGTGGATCTCCCCGTGGGGAACGTCCCCCTCTACCAGGCCTTCTGCGAGGCGGCCCGCAAGT
ACGGTGATCCCAATAAACTGGATCCGGAGATGCTCTTCGACCTGATCGAAAAGCAGTGCGAGGACGGCATGGCCTTCATG
GCGGTCCACTGCGGCATCAACCTCTATACCATCGAGCGCCTGAAGCGGCAGGGGTACCGCTACGGCGGCCTCGTCTCCAA
GGGGGGGGTCAGCATGGTGGCCTGGATGATGGCCAACAGGCGGGAAAATCCCCTCTACGAGCAATTCGACCGGGTAACTT
CGATCCTCAGGAAATACGACACGGTCCTCTCGCTGGGGAACGGGCTGCGGGCTGGCGCCATCCACGACTCCTCGGACCGG
GCCCAGATCCAGGAGCTCCTCATTAACTGCGAGCTGGCTGAACTGGGGCGCGAGATGGGGTGCCAGATGCTCGTGGAGGG
GCCGGGGCACGTGCCGCTCGACGAGGTGGAGGGGAACATCCAGCTCCAGAAGCGGATGAGCGGCGGCGCCCCCTACTACA
TGCTGGGTCCCATCTCCACCGACGTGGCCCCCGGCTTCGACCACATCACCGCCGCCATCGGCGCGGCCCAGTCCTCCCGC
TACGGCGCCGACCTCATCTGCTACATCACTCCGGCCGAGCACCTGGCCCTCCCCAACGAGGAGGATGTCCGCCAGGGGGT
GAAGGCAGCGAAGATCGCGGCCTACATCGGCGACATGAACAAGTACCCGGAACGGGGCCGGGAGCGGGACAAGGAGATGT
CCAAGGCCCGCCGCGACCTGGATTGGAAGAAGCAGTTCGAGCTGGCCCTCTTCCCGGAGGATGCCAAAGCCATCCGTGCC
AGCCGCACCCCCGAGGACGAGGCCACCTGCACCATGTGCGGCGACTTCTGCGCCTCGCGCGGGGCGGGGAAGCTTTTTGC
GGCGGATCTGCGGGGGGATAAGATTTAA

Upstream 100 bases:

>100_bases
ACTGTCTTGCCTCCTACGTCCATATCCACTTCGGGAGTTGCCCGGATATGGCGGAGGCGTTCGTGAACCATTGCCGAAAC
TACGCACAAAGGAGCCATTC

Downstream 100 bases:

>100_bases
GTCGCGAATAACACGGAGAGACGGAGAGCACAGAGAAAAGAGCCGTGCTATGTGAATTATCCAAGGTGCTACCTGCAATT
CTCGCATGTGTAAAATATGT

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 1; HMP-P synthase 1; HMP-phosphate synthase 1; HMPP synthase 1; Thiamine biosynthesis protein thiC 1

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MKTQIEIAREGTISSQMMAVAAEEHVSPDYVRQMVAEGKIVIPGNHSRKPRAVGIGKGLRTKVNASIGTSSDIIDYGAEV
RKALAAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGNVPLYQAFCEAARKYGDPNKLDPEMLFDLIEKQCEDGMAFM
AVHCGINLYTIERLKRQGYRYGGLVSKGGVSMVAWMMANRRENPLYEQFDRVTSILRKYDTVLSLGNGLRAGAIHDSSDR
AQIQELLINCELAELGREMGCQMLVEGPGHVPLDEVEGNIQLQKRMSGGAPYYMLGPISTDVAPGFDHITAAIGAAQSSR
YGADLICYITPAEHLALPNEEDVRQGVKAAKIAAYIGDMNKYPERGRERDKEMSKARRDLDWKKQFELALFPEDAKAIRA
SRTPEDEATCTMCGDFCASRGAGKLFAADLRGDKI

Sequences:

>Translated_435_residues
MKTQIEIAREGTISSQMMAVAAEEHVSPDYVRQMVAEGKIVIPGNHSRKPRAVGIGKGLRTKVNASIGTSSDIIDYGAEV
RKALAAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGNVPLYQAFCEAARKYGDPNKLDPEMLFDLIEKQCEDGMAFM
AVHCGINLYTIERLKRQGYRYGGLVSKGGVSMVAWMMANRRENPLYEQFDRVTSILRKYDTVLSLGNGLRAGAIHDSSDR
AQIQELLINCELAELGREMGCQMLVEGPGHVPLDEVEGNIQLQKRMSGGAPYYMLGPISTDVAPGFDHITAAIGAAQSSR
YGADLICYITPAEHLALPNEEDVRQGVKAAKIAAYIGDMNKYPERGRERDKEMSKARRDLDWKKQFELALFPEDAKAIRA
SRTPEDEATCTMCGDFCASRGAGKLFAADLRGDKI
>Mature_435_residues
MKTQIEIAREGTISSQMMAVAAEEHVSPDYVRQMVAEGKIVIPGNHSRKPRAVGIGKGLRTKVNASIGTSSDIIDYGAEV
RKALAAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGNVPLYQAFCEAARKYGDPNKLDPEMLFDLIEKQCEDGMAFM
AVHCGINLYTIERLKRQGYRYGGLVSKGGVSMVAWMMANRRENPLYEQFDRVTSILRKYDTVLSLGNGLRAGAIHDSSDR
AQIQELLINCELAELGREMGCQMLVEGPGHVPLDEVEGNIQLQKRMSGGAPYYMLGPISTDVAPGFDHITAAIGAAQSSR
YGADLICYITPAEHLALPNEEDVRQGVKAAKIAAYIGDMNKYPERGRERDKEMSKARRDLDWKKQFELALFPEDAKAIRA
SRTPEDEATCTMCGDFCASRGAGKLFAADLRGDKI

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=451, Percent_Identity=35.6984478935698, Blast_Score=291, Evalue=7e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC1_GEOMG (Q39YG0)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383439.1
- STRING:   Q39YG0
- GeneID:   3739838
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0471
- NMPDR:   fig|269799.3.peg.499
- eggNOG:   COG0422
- HOGENOM:   HBG285234
- OMA:   VPLYQAF
- ProtClustDB:   PRK13352
- BioCyc:   GMET269799:GMET_0471-MONOMER
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 47671; Mature: 47671

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: BINDING 95-95 BINDING 124-124 BINDING 163-163 BINDING 266-266 BINDING 293-293

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTQIEIAREGTISSQMMAVAAEEHVSPDYVRQMVAEGKIVIPGNHSRKPRAVGIGKGLR
CCCCEEEHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCCCCCCCEEECCCCCC
TKVNASIGTSSDIIDYGAEVRKALAAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGN
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHHHHCCCCCC
VPLYQAFCEAARKYGDPNKLDPEMLFDLIEKQCEDGMAFMAVHCGINLYTIERLKRQGYR
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHCCCCHHHHHHHHHCCCC
YGGLVSKGGVSMVAWMMANRRENPLYEQFDRVTSILRKYDTVLSLGNGLRAGAIHDSSDR
CCCEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHH
AQIQELLINCELAELGREMGCQMLVEGPGHVPLDEVEGNIQLQKRMSGGAPYYMLGPIST
HHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCHHHCCCCEEEEEHHCCCCCEEEECCCCC
DVAPGFDHITAAIGAAQSSRYGADLICYITPAEHLALPNEEDVRQGVKAAKIAAYIGDMN
CCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KYPERGRERDKEMSKARRDLDWKKQFELALFPEDAKAIRASRTPEDEATCTMCGDFCASR
HCHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHCC
GAGKLFAADLRGDKI
CCCCEEEECCCCCCC
>Mature Secondary Structure
MKTQIEIAREGTISSQMMAVAAEEHVSPDYVRQMVAEGKIVIPGNHSRKPRAVGIGKGLR
CCCCEEEHHCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCEEECCCCCCCCCEEECCCCCC
TKVNASIGTSSDIIDYGAEVRKALAAQEAGADTLMELSVGGDLDRVRREVIAAVDLPVGN
EEECCCCCCCHHHHHHHHHHHHHHHHHHCCCHHEEEEECCCCHHHHHHHHHHHHCCCCCC
VPLYQAFCEAARKYGDPNKLDPEMLFDLIEKQCEDGMAFMAVHCGINLYTIERLKRQGYR
CHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCEEEHHHCCCCHHHHHHHHHCCCC
YGGLVSKGGVSMVAWMMANRRENPLYEQFDRVTSILRKYDTVLSLGNGLRAGAIHDSSDR
CCCEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHH
AQIQELLINCELAELGREMGCQMLVEGPGHVPLDEVEGNIQLQKRMSGGAPYYMLGPIST
HHHHHHHHCCCHHHHHHHCCCEEEECCCCCCCHHHCCCCEEEEEHHCCCCCEEEECCCCC
DVAPGFDHITAAIGAAQSSRYGADLICYITPAEHLALPNEEDVRQGVKAAKIAAYIGDMN
CCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
KYPERGRERDKEMSKARRDLDWKKQFELALFPEDAKAIRASRTPEDEATCTMCGDFCASR
HCHHHHHHHHHHHHHHHHCCCHHHCCEEEECCCHHHHHHCCCCCCCCCHHHHHHHHHHCC
GAGKLFAADLRGDKI
CCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA