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The map label for this gene is cobB [H]

Identifier: 78221691

GI number: 78221691

Start: 516914

End: 518302

Strand: Direct

Name: cobB [H]

Synonym: Gmet_0470

Alternate gene names: 78221691

Gene position: 516914-518302 (Clockwise)

Preceding gene: 78221690

Following gene: 78221692

Centisome position: 12.93

GC content: 66.02

Gene sequence:

>1389_bases
ATGAAATCAATCATCATCGCAGCGCCCCACAGCGGGTCGGGCAAGACGACCATCACCCTCGGCATTATGGAGTGCCTGAA
GCGGCGCGGACTCACGGTGGCCCCCTTCAAGGTGGGGCCCGACTTCATCGATCCCGGTTATCACCGTCTCATCTGCGGCC
GTCCCTCCGTCAACCTGGACGGCTGGATGTGCGGCGGCGACTTCGTGCAGGAGACCTTCTTTCACCACGCCGCCGGCGCC
GACATCGCTGTCGTCGAGGGGGTCATGGGGCTCTTTGACGGCATCGGCGGCGTGTCGGACGAGGGGAGCACCGCGGAGGT
GGCGAAGCTCCTGGGGGGGCCGGTGGTCCTTGTGGCGGACGCCAAGGGGCAGGCCCGCAGCGTCGCCGCCCTGGTGAAGG
GGTTTGCCGATTTCGACCCCGAGGTGAGGGTCGTGGGGGTCATCTTCAATAACGTGGCGAGCGATGCCCACGGCCGCATC
CTGCGGGAGGCCGTGGAAAGTCATCTCCCCGACGTGCGGGTTTTGGGGTGCATCCCCCGCGACGAGAGCCTCTCCATCCC
CTCGCGGCACCTGGGGCTCTTTACCGTTGATGAGAACCCCCTCCCGGCGGAGTTTCTCGATCACCTCGTGGAGGTGGTCC
GGGAGCATGTGGATCTGGGGATGCTCTGGAGCGTGGCCACCCTTCAGGGGGCGTCTGATGTAAAGAGGCGGCAGGATGCG
CCGCGCCGGGAACCGGTCCGGATCGGCGTTGCCCGCGATGCCGCCTTCTGCTTCGTCTACGAGGACAATCTGCGGCTCCT
GCGTGAGGCCGGCGCGGAGCTGGTGGAGTTCTCTCCCCTGGCCGACAGGGAGCTTCCCGAAGGGGTTGCGGGGCTCTACC
TCCCCGGCGGGTACCCGGAGGTTTTCGCCGACGTCCTGGCAGACAACACCCCCATGAAGGATGCGGTGCGCGGGGCGGTG
GTCGGGGAGATGCCGGTCTATGCCGAGTGCGGCGGGTTCATCTACCTTTCCCGGGGGGTGACTGGCATGCGGGATGATCT
GGAATCGTTCAACGAATTTGTCGGCATCTTCCCCGTCGCCACCCTGATGCTCCCCCGCCGCAAGGCCCTGGGGTATCGGG
AGGTGGAGCTTGCCGCAGACTCCATCATCGGCCCCGTTGGCGCCATGGCCCGGGGGCACGAGTTCCACTACTCGGAGATG
GAGGCGATGCCCCCCGAGGTGGAGCGCCTCTACCGGGTGCGCCGGGCCGGCATCGACTTGGGGACGGAAGGGTATCGCCA
CCGGAACTGTCTTGCCTCCTACGTCCATATCCACTTCGGGAGTTGCCCGGATATGGCGGAGGCGTTCGTGAACCATTGCC
GAAACTACGCACAAAGGAGCCATTCATGA

Upstream 100 bases:

>100_bases
TCGAGCCTTTTTGCCATCGAGCAGACCTACTGTTGCCTGAACATCATTGACTACTTCGCCGACGGCAACACCGTCGTGAA
GCTGGTGAACGGCTGACGTC

Downstream 100 bases:

>100_bases
AAACCCAGATCGAAATCGCCCGCGAAGGGACCATTTCTTCCCAGATGATGGCCGTGGCAGCCGAAGAGCACGTTTCCCCC
GACTATGTCCGGCAGATGGT

Product: cobyrinic acid a,c-diamide synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKSIIIAAPHSGSGKTTITLGIMECLKRRGLTVAPFKVGPDFIDPGYHRLICGRPSVNLDGWMCGGDFVQETFFHHAAGA
DIAVVEGVMGLFDGIGGVSDEGSTAEVAKLLGGPVVLVADAKGQARSVAALVKGFADFDPEVRVVGVIFNNVASDAHGRI
LREAVESHLPDVRVLGCIPRDESLSIPSRHLGLFTVDENPLPAEFLDHLVEVVREHVDLGMLWSVATLQGASDVKRRQDA
PRREPVRIGVARDAAFCFVYEDNLRLLREAGAELVEFSPLADRELPEGVAGLYLPGGYPEVFADVLADNTPMKDAVRGAV
VGEMPVYAECGGFIYLSRGVTGMRDDLESFNEFVGIFPVATLMLPRRKALGYREVELAADSIIGPVGAMARGHEFHYSEM
EAMPPEVERLYRVRRAGIDLGTEGYRHRNCLASYVHIHFGSCPDMAEAFVNHCRNYAQRSHS

Sequences:

>Translated_462_residues
MKSIIIAAPHSGSGKTTITLGIMECLKRRGLTVAPFKVGPDFIDPGYHRLICGRPSVNLDGWMCGGDFVQETFFHHAAGA
DIAVVEGVMGLFDGIGGVSDEGSTAEVAKLLGGPVVLVADAKGQARSVAALVKGFADFDPEVRVVGVIFNNVASDAHGRI
LREAVESHLPDVRVLGCIPRDESLSIPSRHLGLFTVDENPLPAEFLDHLVEVVREHVDLGMLWSVATLQGASDVKRRQDA
PRREPVRIGVARDAAFCFVYEDNLRLLREAGAELVEFSPLADRELPEGVAGLYLPGGYPEVFADVLADNTPMKDAVRGAV
VGEMPVYAECGGFIYLSRGVTGMRDDLESFNEFVGIFPVATLMLPRRKALGYREVELAADSIIGPVGAMARGHEFHYSEM
EAMPPEVERLYRVRRAGIDLGTEGYRHRNCLASYVHIHFGSCPDMAEAFVNHCRNYAQRSHS
>Mature_462_residues
MKSIIIAAPHSGSGKTTITLGIMECLKRRGLTVAPFKVGPDFIDPGYHRLICGRPSVNLDGWMCGGDFVQETFFHHAAGA
DIAVVEGVMGLFDGIGGVSDEGSTAEVAKLLGGPVVLVADAKGQARSVAALVKGFADFDPEVRVVGVIFNNVASDAHGRI
LREAVESHLPDVRVLGCIPRDESLSIPSRHLGLFTVDENPLPAEFLDHLVEVVREHVDLGMLWSVATLQGASDVKRRQDA
PRREPVRIGVARDAAFCFVYEDNLRLLREAGAELVEFSPLADRELPEGVAGLYLPGGYPEVFADVLADNTPMKDAVRGAV
VGEMPVYAECGGFIYLSRGVTGMRDDLESFNEFVGIFPVATLMLPRRKALGYREVELAADSIIGPVGAMARGHEFHYSEM
EAMPPEVERLYRVRRAGIDLGTEGYRHRNCLASYVHIHFGSCPDMAEAFVNHCRNYAQRSHS

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: NA

Molecular weight: Translated: 50225; Mature: 50225

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIIIAAPHSGSGKTTITLGIMECLKRRGLTVAPFKVGPDFIDPGYHRLICGRPSVNLD
CCCEEEECCCCCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEECCCCCCCC
GWMCGGDFVQETFFHHAAGADIAVVEGVMGLFDGIGGVSDEGSTAEVAKLLGGPVVLVAD
CEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEC
AKGQARSVAALVKGFADFDPEVRVVGVIFNNVASDAHGRILREAVESHLPDVRVLGCIPR
CCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCC
DESLSIPSRHLGLFTVDENPLPAEFLDHLVEVVREHVDLGMLWSVATLQGASDVKRRQDA
CCCCCCCCHHCEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHCC
PRREPVRIGVARDAAFCFVYEDNLRLLREAGAELVEFSPLADRELPEGVAGLYLPGGYPE
CCCCCEEEEEECCCEEEEEEECCHHHHHHCCCHHEECCCCCCCCCCCCCCEEECCCCCHH
VFADVLADNTPMKDAVRGAVVGEMPVYAECGGFIYLSRGVTGMRDDLESFNEFVGIFPVA
HHHHHHCCCCCHHHHHHHHHHCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
TLMLPRRKALGYREVELAADSIIGPVGAMARGHEFHYSEMEAMPPEVERLYRVRRAGIDL
HHHHHHHHHCCCHHHHHHHHHHHCCHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
GTEGYRHRNCLASYVHIHFGSCPDMAEAFVNHCRNYAQRSHS
CCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSIIIAAPHSGSGKTTITLGIMECLKRRGLTVAPFKVGPDFIDPGYHRLICGRPSVNLD
CCCEEEECCCCCCCCEEEEHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEECCCCCCCC
GWMCGGDFVQETFFHHAAGADIAVVEGVMGLFDGIGGVSDEGSTAEVAKLLGGPVVLVAD
CEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEEC
AKGQARSVAALVKGFADFDPEVRVVGVIFNNVASDAHGRILREAVESHLPDVRVLGCIPR
CCCCHHHHHHHHHHHHCCCCCEEEEEEEHHHHHCHHHHHHHHHHHHHHCCCEEEEEECCC
DESLSIPSRHLGLFTVDENPLPAEFLDHLVEVVREHVDLGMLWSVATLQGASDVKRRQDA
CCCCCCCCHHCEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHCC
PRREPVRIGVARDAAFCFVYEDNLRLLREAGAELVEFSPLADRELPEGVAGLYLPGGYPE
CCCCCEEEEEECCCEEEEEEECCHHHHHHCCCHHEECCCCCCCCCCCCCCEEECCCCCHH
VFADVLADNTPMKDAVRGAVVGEMPVYAECGGFIYLSRGVTGMRDDLESFNEFVGIFPVA
HHHHHHCCCCCHHHHHHHHHHCCCCCEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
TLMLPRRKALGYREVELAADSIIGPVGAMARGHEFHYSEMEAMPPEVERLYRVRRAGIDL
HHHHHHHHHCCCHHHHHHHHHHHCCHHHHHCCCCCCHHHHHCCCHHHHHHHHHHHHCCCC
GTEGYRHRNCLASYVHIHFGSCPDMAEAFVNHCRNYAQRSHS
CCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA