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The map label for this gene is cobS [H]

Identifier: 78221689

GI number: 78221689

Start: 515487

End: 516293

Strand: Direct

Name: cobS [H]

Synonym: Gmet_0468

Alternate gene names: 78221689

Gene position: 515487-516293 (Clockwise)

Preceding gene: 78221688

Following gene: 78221690

Centisome position: 12.9

GC content: 65.18

Gene sequence:

>807_bases
ATGAATATGCTGAAGTCGCATGAGCAGAAGAGGTTTTTTACCGGTCCCCGGTCCCCGGTCCCCAGTCCCCGCCTTTTCTT
TATCGCCTTCCAGTTCCTGACCATCATCCCCCTGCCGTTCACCCTCCGTTGGGAGGAGAACGACCTGGGGCGCTCCATGG
CGTTCTTTCCCCTGGCGGGGCTCACCATAGGCGGGCTCCTGGCCGGGGCTGACTTTCTCCTGGCGGAGGTTCTGCCCCGC
CCGATTGAGGACCTCCTCCTGGTGGCGCTCCTGGCGGCCATGACCGGGGCGCTCCACCACGACGGGCTCGCCGATGTCTG
CGACGGCCTGGCTGCCCGGGGGAGCCGGGACCGTTTCCTGGCGGTGATGAAGGATTCGCGCATCGGCGCCGTGGGGGTGG
TGGGGCTCGTGCTGGGGCTTCTCCTCAAGTACCAGGCCCTCCTGGCCCTTTCCCCCGAGGTGAAGCGCCAGGTCCTCTTC
TTCTTCCCGGCCGTGGCCCGCTTCGCCCAGGTGCAGATGACCGTCGGTTCCCGGCGGGCTCGGGAGGACGGCCTCGGCGC
CGCCTGTATCGCCGGTGCCGGTGTGACACAGCTTCTGGTGGCTGGGGCCATAACTCTTGCTGCGACGTATTTTCTTCTCG
GCATGAAGGGGATCGGTTGCTGGGCGGTCCTCTCCCTCTTCACGGCCGGGACAAAGGCCTGGTCCCACCGGCGCCTCGGC
GGGGTGACCGGCGATGTCATCGGCTGTGCCAGTGAGTTGAACGAAATTATCTGCCTGCTGATTATTGTGGGCATGCAGAA
GATGTAA

Upstream 100 bases:

>100_bases
GCCACCTTCGCGGAGGCCGGCGTGGAAAAAGGCCGGGACCAGTAACCGGGGACCAGGGACTGGGAAAACCTTTATCCAAC
CGATGAACGGGATCAGGAAC

Downstream 100 bases:

>100_bases
GGAAAGCGACATGACACCAAAAACACGGATTCATCTCATCCGCCACGGCGAAGTTGAAGGGGCCGGGCCGGTGCGTCGTT
ATAACGGCCATACCGACGTG

Product: cobalamin-5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNMLKSHEQKRFFTGPRSPVPSPRLFFIAFQFLTIIPLPFTLRWEENDLGRSMAFFPLAGLTIGGLLAGADFLLAEVLPR
PIEDLLLVALLAAMTGALHHDGLADVCDGLAARGSRDRFLAVMKDSRIGAVGVVGLVLGLLLKYQALLALSPEVKRQVLF
FFPAVARFAQVQMTVGSRRAREDGLGAACIAGAGVTQLLVAGAITLAATYFLLGMKGIGCWAVLSLFTAGTKAWSHRRLG
GVTGDVIGCASELNEIICLLIIVGMQKM

Sequences:

>Translated_268_residues
MNMLKSHEQKRFFTGPRSPVPSPRLFFIAFQFLTIIPLPFTLRWEENDLGRSMAFFPLAGLTIGGLLAGADFLLAEVLPR
PIEDLLLVALLAAMTGALHHDGLADVCDGLAARGSRDRFLAVMKDSRIGAVGVVGLVLGLLLKYQALLALSPEVKRQVLF
FFPAVARFAQVQMTVGSRRAREDGLGAACIAGAGVTQLLVAGAITLAATYFLLGMKGIGCWAVLSLFTAGTKAWSHRRLG
GVTGDVIGCASELNEIICLLIIVGMQKM
>Mature_268_residues
MNMLKSHEQKRFFTGPRSPVPSPRLFFIAFQFLTIIPLPFTLRWEENDLGRSMAFFPLAGLTIGGLLAGADFLLAEVLPR
PIEDLLLVALLAAMTGALHHDGLADVCDGLAARGSRDRFLAVMKDSRIGAVGVVGLVLGLLLKYQALLALSPEVKRQVLF
FFPAVARFAQVQMTVGSRRAREDGLGAACIAGAGVTQLLVAGAITLAATYFLLGMKGIGCWAVLSLFTAGTKAWSHRRLG
GVTGDVIGCASELNEIICLLIIVGMQKM

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=247, Percent_Identity=35.6275303643725, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 28684; Mature: 28684

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMLKSHEQKRFFTGPRSPVPSPRLFFIAFQFLTIIPLPFTLRWEENDLGRSMAFFPLAG
CCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
LTIGGLLAGADFLLAEVLPRPIEDLLLVALLAAMTGALHHDGLADVCDGLAARGSRDRFL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHEE
AVMKDSRIGAVGVVGLVLGLLLKYQALLALSPEVKRQVLFFFPAVARFAQVQMTVGSRRA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REDGLGAACIAGAGVTQLLVAGAITLAATYFLLGMKGIGCWAVLSLFTAGTKAWSHRRLG
HHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
GVTGDVIGCASELNEIICLLIIVGMQKM
CCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNMLKSHEQKRFFTGPRSPVPSPRLFFIAFQFLTIIPLPFTLRWEENDLGRSMAFFPLAG
CCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHH
LTIGGLLAGADFLLAEVLPRPIEDLLLVALLAAMTGALHHDGLADVCDGLAARGSRDRFL
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHEE
AVMKDSRIGAVGVVGLVLGLLLKYQALLALSPEVKRQVLFFFPAVARFAQVQMTVGSRRA
EEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REDGLGAACIAGAGVTQLLVAGAITLAATYFLLGMKGIGCWAVLSLFTAGTKAWSHRRLG
HHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
GVTGDVIGCASELNEIICLLIIVGMQKM
CCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA