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The map label for this gene is kdsB [C]

Identifier: 78221675

GI number: 78221675

Start: 500679

End: 502460

Strand: Reverse

Name: kdsB [C]

Synonym: Gmet_0454

Alternate gene names: 78221675

Gene position: 502460-500679 (Counterclockwise)

Preceding gene: 78221676

Following gene: 78221674

Centisome position: 12.57

GC content: 54.43

Gene sequence:

>1782_bases
ATGGCTAAAAAGAAAATTGTAGCTGTCATTCAAGCCCGCATGGGTTCTTCGCGCCTGCCGGGAAAGGTGCTCATGACAGT
GGCGGGCAAACCGCTGCTTGAGCATATTATTGACCGCCTTCGCCGCAGCCGCACTCTCGATGACATAGTTATCGCCACAT
CGGACAAGCCCTGCGACGACCCTCTGGTTGACTTCGCCCAAACGCGAGGGGTTACCCTGGTGAGAGGCTCCGAGGACAAT
GTCCTCTTGCGCTTCAACAAGGCGGCAGAAGAGGTTGATGCCGATGTCATCATTCGTGTTACTGGCGATGCTCCCTTGGT
GGATCCCGAGGTAATGGACCGGTTGGTGCGAACCCTCTTGCAGGAAGGGGCTGAGTACTGCACTATCGAAGCAGGGATTA
ATTCCATTCACGAAGGGTTCTGCACCTTCACGCGTGGAGCCCTGAAGAGGCTCATGACCGAAGCCCCCGATGATCCGGTG
GCCATTGAGCATGTAACGGCCTACTTCATTGCACACCCGGACAAATTTCATATTGCCCGGATTCCAGTCGGCAACGAACA
CCGCTTCAAAGGCGCCCGGCTGTCTGTCGATACTCCGGCGGATGTGCAATTTTTCGATGAACTTTACCGGCTTCTCGGCG
TTCCTGCGGGGGATGCCGATATTGCCGACGTCGTACGACTTCTCAGGAACAATCCTCACCTGCTTCAAATCAATCAACAT
GTCTATCAGAAAAAAGCAACCGACCGGAGCCACAAGGTTCTCATTCGTTGCGATGGCGATAGTCGTACCGGCCTCGGGCA
TGTGGTTCGTTGCCTGGCTCTTGCAGGAGAACTGCGAGAGAAGCAGGGCTGCGGTATAATCTTCGCCATGGCCAGCGGCG
AACCCGGCATGAAAATGGTGCGCCAAGCGGATTTTCCGCTCGAACGGCAGGAAGATGCAGACGAAGGGGAATGGATGGAT
GGACTGATTTCGCGCCATCACCCCGACGTCCTGATACTCGATATACGGAACGATTTGCCCGTGGAAAAAGTCGATTCCTG
GCGCGAGGATGGCATTCTGGTCGTTGGCATAGATGATCCTACGCCTCGACGCCTTGCAGCCGATCTCCTTTTCTATCCTC
CCATCCCTCAGATTTCAGAACTGAATTGGAATAATTTCAACGGTGAGATCTGCAGCGGTTGGGAGTGGGTTTTACTCCGC
AAACAATTCTGCGCGCCCTGCCCCCCGCGCAACAACAATCCTCCAGCACTCCTTCTCACCATGGGAGGGAGTGACCCTGC
GAATTTCACTGTTAGGGTTCTCCGCGTCCTTGACGAATTGCAGAATGATTTTGAAACGATCGTGGTTATAGGGGCAGGTT
TTGTCCATGAAGAGGACCTGCAGCAGTTTCTCTCCCAAGCCCGACGCCACTACCGTATCGAACGGGAAGTAAGCGACATG
GCATCACTCATGCGACAAGCCGATCTGGCCATCGCGTCTTTTGGTGTAACAGCTTACGAGTTGGCAGCATCCGGCGTACC
GGCAGTGTATTTATGCCTGACTCCGGATCACGCCCGTTCGGCCTCAAAGTTTGTCGATGACCGGCTTGCTTTCAGTTGCG
GCGTACTCGAAGAATGCAGCAATGAGAGGATTATCGAATCGACATTATCACTTCTTGACTCTCGGGAACTCAGACACGAA
GTGGGACGCCGCGCCTGCGCCACTGTCGATGGGTACGGTGCGACGCGGATAGCCACGAAGATCGTTGAAAAACTGGAAAG
GATGCATTCTTCTCATGGCTGA

Upstream 100 bases:

>100_bases
GGAGAAAAGGTGCCCACCCCCGTTGAGTTCCCTGACCGCGAATGGCGTGCCGACCCTTCCGACGGCTTGAGACCTTTTCT
GCATGTAAGGAACGAATGGA

Downstream 100 bases:

>100_bases
AAAGAAAACCTGGTGTGATCATGAAGGTGAAGTCTGCGACTCAGTCAAGGGATTTGATGTCATCGACTGCAGCAAATGCG
GTTTTAAGCATATCGTTCCG

Product: acylneuraminate cytidylyltransferase

Products: CMP-3-deoxy-D-manno-octulosonate; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MAKKKIVAVIQARMGSSRLPGKVLMTVAGKPLLEHIIDRLRRSRTLDDIVIATSDKPCDDPLVDFAQTRGVTLVRGSEDN
VLLRFNKAAEEVDADVIIRVTGDAPLVDPEVMDRLVRTLLQEGAEYCTIEAGINSIHEGFCTFTRGALKRLMTEAPDDPV
AIEHVTAYFIAHPDKFHIARIPVGNEHRFKGARLSVDTPADVQFFDELYRLLGVPAGDADIADVVRLLRNNPHLLQINQH
VYQKKATDRSHKVLIRCDGDSRTGLGHVVRCLALAGELREKQGCGIIFAMASGEPGMKMVRQADFPLERQEDADEGEWMD
GLISRHHPDVLILDIRNDLPVEKVDSWREDGILVVGIDDPTPRRLAADLLFYPPIPQISELNWNNFNGEICSGWEWVLLR
KQFCAPCPPRNNNPPALLLTMGGSDPANFTVRVLRVLDELQNDFETIVVIGAGFVHEEDLQQFLSQARRHYRIEREVSDM
ASLMRQADLAIASFGVTAYELAASGVPAVYLCLTPDHARSASKFVDDRLAFSCGVLEECSNERIIESTLSLLDSRELRHE
VGRRACATVDGYGATRIATKIVEKLERMHSSHG

Sequences:

>Translated_593_residues
MAKKKIVAVIQARMGSSRLPGKVLMTVAGKPLLEHIIDRLRRSRTLDDIVIATSDKPCDDPLVDFAQTRGVTLVRGSEDN
VLLRFNKAAEEVDADVIIRVTGDAPLVDPEVMDRLVRTLLQEGAEYCTIEAGINSIHEGFCTFTRGALKRLMTEAPDDPV
AIEHVTAYFIAHPDKFHIARIPVGNEHRFKGARLSVDTPADVQFFDELYRLLGVPAGDADIADVVRLLRNNPHLLQINQH
VYQKKATDRSHKVLIRCDGDSRTGLGHVVRCLALAGELREKQGCGIIFAMASGEPGMKMVRQADFPLERQEDADEGEWMD
GLISRHHPDVLILDIRNDLPVEKVDSWREDGILVVGIDDPTPRRLAADLLFYPPIPQISELNWNNFNGEICSGWEWVLLR
KQFCAPCPPRNNNPPALLLTMGGSDPANFTVRVLRVLDELQNDFETIVVIGAGFVHEEDLQQFLSQARRHYRIEREVSDM
ASLMRQADLAIASFGVTAYELAASGVPAVYLCLTPDHARSASKFVDDRLAFSCGVLEECSNERIIESTLSLLDSRELRHE
VGRRACATVDGYGATRIATKIVEKLERMHSSHG
>Mature_592_residues
AKKKIVAVIQARMGSSRLPGKVLMTVAGKPLLEHIIDRLRRSRTLDDIVIATSDKPCDDPLVDFAQTRGVTLVRGSEDNV
LLRFNKAAEEVDADVIIRVTGDAPLVDPEVMDRLVRTLLQEGAEYCTIEAGINSIHEGFCTFTRGALKRLMTEAPDDPVA
IEHVTAYFIAHPDKFHIARIPVGNEHRFKGARLSVDTPADVQFFDELYRLLGVPAGDADIADVVRLLRNNPHLLQINQHV
YQKKATDRSHKVLIRCDGDSRTGLGHVVRCLALAGELREKQGCGIIFAMASGEPGMKMVRQADFPLERQEDADEGEWMDG
LISRHHPDVLILDIRNDLPVEKVDSWREDGILVVGIDDPTPRRLAADLLFYPPIPQISELNWNNFNGEICSGWEWVLLRK
QFCAPCPPRNNNPPALLLTMGGSDPANFTVRVLRVLDELQNDFETIVVIGAGFVHEEDLQQFLSQARRHYRIEREVSDMA
SLMRQADLAIASFGVTAYELAASGVPAVYLCLTPDHARSASKFVDDRLAFSCGVLEECSNERIIESTLSLLDSRELRHEV
GRRACATVDGYGATRIATKIVEKLERMHSSHG

Specific function: Activates Kdo (A Required 8-Carbon Sugar) For Incorporation Into Bacterial Lipopolysaccharide In Gram-Negative Bacteria (By Similarity). [C]

COG id: COG1861

COG function: function code M; Spore coat polysaccharide biosynthesis protein F, CMP-KDO synthetase homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003329 [H]

Pfam domain/function: PF02348 CTP_transf_3 [H]

EC number: 2.7.7.38 [C]

Molecular weight: Translated: 66039; Mature: 65908

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKIVAVIQARMGSSRLPGKVLMTVAGKPLLEHIIDRLRRSRTLDDIVIATSDKPCDD
CCCHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
PLVDFAQTRGVTLVRGSEDNVLLRFNKAAEEVDADVIIRVTGDAPLVDPEVMDRLVRTLL
HHHHHHHHCCEEEEECCCCCEEEEECCHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHH
QEGAEYCTIEAGINSIHEGFCTFTRGALKRLMTEAPDDPVAIEHVTAYFIAHPDKFHIAR
HCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHEEEEEECCCCEEEEE
IPVGNEHRFKGARLSVDTPADVQFFDELYRLLGVPAGDADIADVVRLLRNNPHLLQINQH
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEHHH
VYQKKATDRSHKVLIRCDGDSRTGLGHVVRCLALAGELREKQGCGIIFAMASGEPGMKMV
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
RQADFPLERQEDADEGEWMDGLISRHHPDVLILDIRNDLPVEKVDSWREDGILVVGIDDP
HHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHCHHHCCEEEEECCCC
TPRRLAADLLFYPPIPQISELNWNNFNGEICSGWEWVLLRKQFCAPCPPRNNNPPALLLT
CHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEE
MGGSDPANFTVRVLRVLDELQNDFETIVVIGAGFVHEEDLQQFLSQARRHYRIEREVSDM
ECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASLMRQADLAIASFGVTAYELAASGVPAVYLCLTPDHARSASKFVDDRLAFSCGVLEECS
HHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHCC
NERIIESTLSLLDSRELRHEVGRRACATVDGYGATRIATKIVEKLERMHSSHG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKKIVAVIQARMGSSRLPGKVLMTVAGKPLLEHIIDRLRRSRTLDDIVIATSDKPCDD
CCHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCC
PLVDFAQTRGVTLVRGSEDNVLLRFNKAAEEVDADVIIRVTGDAPLVDPEVMDRLVRTLL
HHHHHHHHCCEEEEECCCCCEEEEECCHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHH
QEGAEYCTIEAGINSIHEGFCTFTRGALKRLMTEAPDDPVAIEHVTAYFIAHPDKFHIAR
HCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHEEEEEECCCCEEEEE
IPVGNEHRFKGARLSVDTPADVQFFDELYRLLGVPAGDADIADVVRLLRNNPHLLQINQH
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEEHHH
VYQKKATDRSHKVLIRCDGDSRTGLGHVVRCLALAGELREKQGCGIIFAMASGEPGMKMV
HHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHH
RQADFPLERQEDADEGEWMDGLISRHHPDVLILDIRNDLPVEKVDSWREDGILVVGIDDP
HHCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHCHHHCCEEEEECCCC
TPRRLAADLLFYPPIPQISELNWNNFNGEICSGWEWVLLRKQFCAPCPPRNNNPPALLLT
CHHHHHHHHHCCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEE
MGGSDPANFTVRVLRVLDELQNDFETIVVIGAGFVHEEDLQQFLSQARRHYRIEREVSDM
ECCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHH
ASLMRQADLAIASFGVTAYELAASGVPAVYLCLTPDHARSASKFVDDRLAFSCGVLEECS
HHHHHHHHHHHHHCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCHHHHCC
NERIIESTLSLLDSRELRHEVGRRACATVDGYGATRIATKIVEKLERMHSSHG
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: GSH [C]

Metal ions: Ba2+; Ca2+; Cd2+; Co2+; Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.34 {dCTP}} 0.88 {UTP}} 0.8 {3-deoxy-manno-octulosonate}} 0.39 {3-deoxy-manno-octulosonate}} 0.29 {3-deoxy-manno-octulosonate}} 0.22 {CTP}} 0.2 {CTP}} [C]

Substrates: CTP; 3-Deoxy-D-manno-2-octulosonate [C]

Specific reaction: CTP + 3-Deoxy-D-manno-2-octulosonate --> CMP-3-deoxy-D-manno-octulosonate + Diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 2,6-Anhydro-3-deoxy-D-glycero-D-talo-octanoate; Diphosphate; Hg2+; 3-deoxy-manno-octulosonate 8-phosphate; N-acetyl neuraminate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]