Click here to switch to the map view.

The map label for this gene is hemK [H]

Identifier: 78221602

GI number: 78221602

Start: 425557

End: 426411

Strand: Direct

Name: hemK [H]

Synonym: Gmet_0381

Alternate gene names: 78221602

Gene position: 425557-426411 (Clockwise)

Preceding gene: 78221601

Following gene: 78221603

Centisome position: 10.65

GC content: 65.03

Gene sequence:

>855_bases
ATGACTGAAAAGCCTGAGACCTGGACTATTCGCAGGGTTCTTGACTGGACCAAAGGATACCTGGCGGAGAAAGGCGTTGA
GAACGCCCGTCTGGAGACAGAGTGGCTTCTTTCCGCGGCTCTGGGTCTCGACCGGGTGGGACTCTATGTTAACTTCGACA
AGCCCTTGAACGCCGAAGAACTGTCGGCGGTCCGGGGACTCGTGGCGCGACGGGCCAAGCGGGAACCACTCCAGTACGTC
CTCGGTACCCAGGAGTTCTGCGGCCTCGACTTCGCCGTGACGCCGGCGGTGCTCATCCCCCGGCACGACACCGAGGTGCT
CGTGGAGGAGGCGCTCCGCCGTGCCCCCCACGCCGCCACGGTCCTCGACATCGGCGTCGGGAGCGGCTGCATCGCCGTGG
CTCTGGCAAAGAACCTTCCCGATGCCCAGGTGTGGGGGGTGGAGCAGTCCGCCGGAGCCATCACCCTGGCCCGGCAAAAC
GTTGAGCGCCACGGCGTCCGGGTGATCCTTTGCGCAGGGTCCCTCTTCGAGCCCTTCGCAGACCAGCGGTTCGACCTGAT
CGTCTCCAATCCCCCCTACATCCCCACTGCCGACCTGGATACGCTCCAGCCGGAGGTGCGGGAGTACGAGCCCCGCGCGG
CTCTTGACGGTGGCGCCGACGGTCTCGATTTCTACCGGATCATCGTCCCGGCCGCTTTGGAGCATTTGAACGCGGGCGGG
TGGCTCATGGTCGAACTGGGTATCGGCCAGGCAGAGGCGGTGCTCGGGATGTTTAAGCAGGCCGGTTTCACCGGCTGCTT
TACGGCCAAGGATCTCAACGGCATCGACCGGGTGGTCGGCGGACGGATTCCGTAG

Upstream 100 bases:

>100_bases
TTGCCGAGGTGGTGGATTCGCTTCGGGCCCATTACCAGATGGAGGCCCTCAAGGCCCAGGCCGAAGCAGCCTGATCCGCA
CGGACGGCGGAGGCGGCACA

Downstream 100 bases:

>100_bases
TGGCGTGATTCATCACGTCCCGGGTGCAACGAATTGCACCCCCACATCAATTTCAGAGGCATTCCATTGGACAAGCTGAT
AATCAAAGGGGGAAAGAAAC

Product: modification methylase HemK

Products: NA

Alternate protein names: M.XfaHemK2P [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTEKPETWTIRRVLDWTKGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNAEELSAVRGLVARRAKREPLQYV
LGTQEFCGLDFAVTPAVLIPRHDTEVLVEEALRRAPHAATVLDIGVGSGCIAVALAKNLPDAQVWGVEQSAGAITLARQN
VERHGVRVILCAGSLFEPFADQRFDLIVSNPPYIPTADLDTLQPEVREYEPRAALDGGADGLDFYRIIVPAALEHLNAGG
WLMVELGIGQAEAVLGMFKQAGFTGCFTAKDLNGIDRVVGGRIP

Sequences:

>Translated_284_residues
MTEKPETWTIRRVLDWTKGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNAEELSAVRGLVARRAKREPLQYV
LGTQEFCGLDFAVTPAVLIPRHDTEVLVEEALRRAPHAATVLDIGVGSGCIAVALAKNLPDAQVWGVEQSAGAITLARQN
VERHGVRVILCAGSLFEPFADQRFDLIVSNPPYIPTADLDTLQPEVREYEPRAALDGGADGLDFYRIIVPAALEHLNAGG
WLMVELGIGQAEAVLGMFKQAGFTGCFTAKDLNGIDRVVGGRIP
>Mature_283_residues
TEKPETWTIRRVLDWTKGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNAEELSAVRGLVARRAKREPLQYVL
GTQEFCGLDFAVTPAVLIPRHDTEVLVEEALRRAPHAATVLDIGVGSGCIAVALAKNLPDAQVWGVEQSAGAITLARQNV
ERHGVRVILCAGSLFEPFADQRFDLIVSNPPYIPTADLDTLQPEVREYEPRAALDGGADGLDFYRIIVPAALEHLNAGGW
LMVELGIGQAEAVLGMFKQAGFTGCFTAKDLNGIDRVVGGRIP

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=266, Percent_Identity=33.0827067669173, Blast_Score=128, Evalue=5e-30,
Organism=Escherichia coli, GI1787463, Length=270, Percent_Identity=35.1851851851852, Blast_Score=147, Evalue=5e-37,
Organism=Escherichia coli, GI87082085, Length=257, Percent_Identity=32.295719844358, Blast_Score=115, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324266, Length=211, Percent_Identity=29.3838862559242, Blast_Score=83, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24582226, Length=239, Percent_Identity=33.0543933054393, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30900; Mature: 30769

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKPETWTIRRVLDWTKGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNAEE
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
LSAVRGLVARRAKREPLQYVLGTQEFCGLDFAVTPAVLIPRHDTEVLVEEALRRAPHAAT
HHHHHHHHHHHHHCCCHHHHHCCHHHHCCCEECCCEEEECCCCHHHHHHHHHHHCCCCEE
VLDIGVGSGCIAVALAKNLPDAQVWGVEQSAGAITLARQNVERHGVRVILCAGSLFEPFA
EEEECCCCCHHHHHHHCCCCCCCEECCCCCCCEEEEEHHHHHHCCCEEEEECCCHHHHHH
DQRFDLIVSNPPYIPTADLDTLQPEVREYEPRAALDGGADGLDFYRIIVPAALEHLNAGG
CCCEEEEECCCCCCCCCCCHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
WLMVELGIGQAEAVLGMFKQAGFTGCFTAKDLNGIDRVVGGRIP
EEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCC
>Mature Secondary Structure 
TEKPETWTIRRVLDWTKGYLAEKGVENARLETEWLLSAALGLDRVGLYVNFDKPLNAEE
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHH
LSAVRGLVARRAKREPLQYVLGTQEFCGLDFAVTPAVLIPRHDTEVLVEEALRRAPHAAT
HHHHHHHHHHHHHCCCHHHHHCCHHHHCCCEECCCEEEECCCCHHHHHHHHHHHCCCCEE
VLDIGVGSGCIAVALAKNLPDAQVWGVEQSAGAITLARQNVERHGVRVILCAGSLFEPFA
EEEECCCCCHHHHHHHCCCCCCCEECCCCCCCEEEEEHHHHHHCCCEEEEECCCHHHHHH
DQRFDLIVSNPPYIPTADLDTLQPEVREYEPRAALDGGADGLDFYRIIVPAALEHLNAGG
CCCEEEEECCCCCCCCCCCHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
WLMVELGIGQAEAVLGMFKQAGFTGCFTAKDLNGIDRVVGGRIP
EEEEEECCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]