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The map label for this gene is prfA

Identifier: 78221601

GI number: 78221601

Start: 424463

End: 425530

Strand: Direct

Name: prfA

Synonym: Gmet_0380

Alternate gene names: 78221601

Gene position: 424463-425530 (Clockwise)

Preceding gene: 78221600

Following gene: 78221602

Centisome position: 10.62

GC content: 63.11

Gene sequence:

>1068_bases
ATGTTCGACAAGATAGAAGAACTTGAAATCCGGTACCAGGAGCTGGAATCGCTCCTGGCCGTCCCGACGGTGATCGCCAA
TCAGCCCGAGTTCCGTAAACTCTCCCGGGAGCACAACGACCTGACGGGCTTGGTCGAGGCCTATCGCCGCTACAAGAAGG
TCCTTGCCGAGATCGAGGGGAACCGGGAACTTCTGGCCGATCCGGAGATGAAGGAGATGGCCGAGGCTGAGCTGGAGGAT
CTGGAGCGGCAACAAGAGGAACTGGAGGGGGAGATCAAGCTGCTCCTTTTGCCGAAGGATCCCAATGACGACCGCAACGT
CATCCTGGAGATCCGCGCCGGCACCGGCGGCGACGAGTCCGCCCTCTTTGCCGGTGACCTCTTCCGGATGTACTCCCGCT
TTGCCGAGCGCAACCGTTGGAAGGTGGAGGTCATGTCCGCCTCCGAGTCGGAACGGGGGGGCTTCAAGGAGGTCGTGGCC
CTTATTGAAGGGCAGGCGGTCTTTGCCAAGCTCAAGTACGAGTCGGGGACCCATCGGGTACAGCGGGTGCCGGAGACCGA
GGCCCAGGGACGGATCCACACCAGTGCCTGCACCGTTGCGGTCCTCCCCGAGGCCGAGGATATCGAGATTGACATCAACC
CGGTGGACCTCAAGATCGACGTCTACCGGGCCTCAGGGGCAGGCGGCCAGCACGTCAACAAGACCGAGTCGGCGGTCCGG
ATCACCCACCTTCCCACCGGCATCGTGGTGGAGTGCCAGGACGAACGGAGCCAGATCAAGAACCGGTCCAAGGCCATGAA
GGTCCTCAAATCCAGGATTCTGGACGGCCTCCAGCAGGAGCAGAACGCCCGCATCGCCGCCGACCGCAAGCAGCAGGTGG
GAAGCGGTGACCGGAGCGAGCGGATCCGCACCTACAACTTCCCCCAGGGGCGCATGACCGACCACCGGATCGGCCTCACC
CTCTACCGTCTCGATGCCCTCATGGAGGGGGACATTGCCGAGGTGGTGGATTCGCTTCGGGCCCATTACCAGATGGAGGC
CCTCAAGGCCCAGGCCGAAGCAGCCTGA

Upstream 100 bases:

>100_bases
CATGACCGAGGCCCTGGAAGAGAATGGCGGCTACAAGGACGACCGGCTGGTGGTGTGATGTCCCGCCGGCCCTGATTTTC
GTTTCTGAAGGATTGCGTTG

Downstream 100 bases:

>100_bases
TCCGCACGGACGGCGGAGGCGGCACAATGACTGAAAAGCCTGAGACCTGGACTATTCGCAGGGTTCTTGACTGGACCAAA
GGATACCTGGCGGAGAAAGG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MFDKIEELEIRYQELESLLAVPTVIANQPEFRKLSREHNDLTGLVEAYRRYKKVLAEIEGNRELLADPEMKEMAEAELED
LERQQEELEGEIKLLLLPKDPNDDRNVILEIRAGTGGDESALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEVVA
LIEGQAVFAKLKYESGTHRVQRVPETEAQGRIHTSACTVAVLPEAEDIEIDINPVDLKIDVYRASGAGGQHVNKTESAVR
ITHLPTGIVVECQDERSQIKNRSKAMKVLKSRILDGLQQEQNARIAADRKQQVGSGDRSERIRTYNFPQGRMTDHRIGLT
LYRLDALMEGDIAEVVDSLRAHYQMEALKAQAEAA

Sequences:

>Translated_355_residues
MFDKIEELEIRYQELESLLAVPTVIANQPEFRKLSREHNDLTGLVEAYRRYKKVLAEIEGNRELLADPEMKEMAEAELED
LERQQEELEGEIKLLLLPKDPNDDRNVILEIRAGTGGDESALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEVVA
LIEGQAVFAKLKYESGTHRVQRVPETEAQGRIHTSACTVAVLPEAEDIEIDINPVDLKIDVYRASGAGGQHVNKTESAVR
ITHLPTGIVVECQDERSQIKNRSKAMKVLKSRILDGLQQEQNARIAADRKQQVGSGDRSERIRTYNFPQGRMTDHRIGLT
LYRLDALMEGDIAEVVDSLRAHYQMEALKAQAEAA
>Mature_355_residues
MFDKIEELEIRYQELESLLAVPTVIANQPEFRKLSREHNDLTGLVEAYRRYKKVLAEIEGNRELLADPEMKEMAEAELED
LERQQEELEGEIKLLLLPKDPNDDRNVILEIRAGTGGDESALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEVVA
LIEGQAVFAKLKYESGTHRVQRVPETEAQGRIHTSACTVAVLPEAEDIEIDINPVDLKIDVYRASGAGGQHVNKTESAVR
ITHLPTGIVVECQDERSQIKNRSKAMKVLKSRILDGLQQEQNARIAADRKQQVGSGDRSERIRTYNFPQGRMTDHRIGLT
LYRLDALMEGDIAEVVDSLRAHYQMEALKAQAEAA

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=283, Percent_Identity=49.1166077738516, Blast_Score=280, Evalue=1e-75,
Organism=Homo sapiens, GI34577120, Length=337, Percent_Identity=39.1691394658754, Blast_Score=241, Evalue=6e-64,
Organism=Homo sapiens, GI166795305, Length=178, Percent_Identity=48.314606741573, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1787462, Length=351, Percent_Identity=57.5498575498575, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI2367172, Length=339, Percent_Identity=39.5280235988201, Blast_Score=211, Evalue=4e-56,
Organism=Caenorhabditis elegans, GI17542784, Length=274, Percent_Identity=39.051094890511, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=39.3939393939394, Blast_Score=243, Evalue=5e-65,
Organism=Drosophila melanogaster, GI19921226, Length=283, Percent_Identity=43.4628975265018, Blast_Score=229, Evalue=2e-60,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_GEOMG (Q39YQ1)

Other databases:

- EMBL:   CP000148
- RefSeq:   YP_383348.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q39YQ1
- SMR:   Q39YQ1
- STRING:   Q39YQ1
- GeneID:   3739746
- GenomeReviews:   CP000148_GR
- KEGG:   gme:Gmet_0380
- NMPDR:   fig|269799.3.peg.392
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q39YQ1
- ProtClustDB:   PRK00591
- BioCyc:   GMET269799:GMET_0380-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40175; Mature: 40175

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDKIEELEIRYQELESLLAVPTVIANQPEFRKLSREHNDLTGLVEAYRRYKKVLAEIEG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
NRELLADPEMKEMAEAELEDLERQQEELEGEIKLLLLPKDPNDDRNVILEIRAGTGGDES
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
ALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEVVALIEGQAVFAKLKYESGTHRV
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHH
QRVPETEAQGRIHTSACTVAVLPEAEDIEIDINPVDLKIDVYRASGAGGQHVNKTESAVR
HCCCCCCCCCEEEECEEEEEECCCCCCCEEECCEEEEEEEEEEECCCCCCCCCCCCCEEE
ITHLPTGIVVECQDERSQIKNRSKAMKVLKSRILDGLQQEQNARIAADRKQQVGSGDRSE
EEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
RIRTYNFPQGRMTDHRIGLTLYRLDALMEGDIAEVVDSLRAHYQMEALKAQAEAA
CCEEECCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MFDKIEELEIRYQELESLLAVPTVIANQPEFRKLSREHNDLTGLVEAYRRYKKVLAEIEG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
NRELLADPEMKEMAEAELEDLERQQEELEGEIKLLLLPKDPNDDRNVILEIRAGTGGDES
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCCCCCCC
ALFAGDLFRMYSRFAERNRWKVEVMSASESERGGFKEVVALIEGQAVFAKLKYESGTHRV
HHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHCCCEEEEEEECCCCCHHH
QRVPETEAQGRIHTSACTVAVLPEAEDIEIDINPVDLKIDVYRASGAGGQHVNKTESAVR
HCCCCCCCCCEEEECEEEEEECCCCCCCEEECCEEEEEEEEEEECCCCCCCCCCCCCEEE
ITHLPTGIVVECQDERSQIKNRSKAMKVLKSRILDGLQQEQNARIAADRKQQVGSGDRSE
EEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCC
RIRTYNFPQGRMTDHRIGLTLYRLDALMEGDIAEVVDSLRAHYQMEALKAQAEAA
CCEEECCCCCCCCCHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA