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The map label for this gene is yagI [H]

Identifier: 78221591

GI number: 78221591

Start: 413211

End: 414008

Strand: Reverse

Name: yagI [H]

Synonym: Gmet_0370

Alternate gene names: 78221591

Gene position: 414008-413211 (Counterclockwise)

Preceding gene: 78221592

Following gene: 78221590

Centisome position: 10.36

GC content: 61.65

Gene sequence:

>798_bases
GTGCAGAAAAAAGACAAATCCGATTACATGATTCTTGCCGTCTCCCACGCCCTCGATCTCCTGGAGCAGTTCCACGGAGA
AGGGGTGTCCGAGCTCGGCGTAACCGAACTGGCAAAGCGGCTCAGGCTCCACAAGAACAACGTATTCCGGCTCCTGGCCA
CCCTGGAGGCCCGGGGGTACGTGGAACAGAACCGGGTCACCGGCAACTACCGGCTCGGACTCAAAACCCTTGAGATCCGC
CAGACCATGATCCGCCAGATGGCGCTCCTCCCCTACGCAAAACCGGTTCTTGAGGAATTGGTCAGGGAATGCGATGAAAC
CGCTTACGTTTCCATCCTTAAGGAAAACCTGAGCATCTACCTCTACGGGGTCGAAAGCCGTGCCACGGTCCGGGTCGTCT
CCCGACTCGGTTCCCGGCTGCCCGCTTACTGCACCGCCGCCGGGAAGGTGATGCTCGCTTCCCTCTCCGAACCGAAGCTT
GAGCGTTACCTGGCCCGCAAGGAACTGGTCCCCTTCACTCCCAACACCATCACCGACGCCACCCGGCTCCGGGAGCACCT
GAAGGAAATCGCCGCCGCCGGCTACGCCATCGACAACGAGGAGCTTGAAATCGGAGTCCGGGGGGTGGCTGCCCCCATCC
GCGACTACCGGACTGAAACGGTCGGGGCGGTCATCATCTCAGGGCCGACCATGAGGTTCTCCGACGAGCGCGTCGCCGCA
GAACTGGTCCCCCTGGCCCAGAAAGCCGCCGAAACCATCTCCGTAAAACTCGGCTATAGCGGGGCCGCAGCGGAATAG

Upstream 100 bases:

>100_bases
TCCCCCTCTGCAACAAGAGTTTTAATCTCTCCTACTGCGGGTTCGATTTGTGATATAAGAGACGGGTTTGCTTTTTCACT
CCACCCGACAGAGGCTCACC

Downstream 100 bases:

>100_bases
GTCCCCCCCAGGGGAGGCGCACCATGCTCAAGGCACTCTTTGCCAGACGTCACCAGGGACACCGAACCATCGCCTACCCC
GCTGGGCCGGCGCAACTGCC

Product: IclR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MQKKDKSDYMILAVSHALDLLEQFHGEGVSELGVTELAKRLRLHKNNVFRLLATLEARGYVEQNRVTGNYRLGLKTLEIR
QTMIRQMALLPYAKPVLEELVRECDETAYVSILKENLSIYLYGVESRATVRVVSRLGSRLPAYCTAAGKVMLASLSEPKL
ERYLARKELVPFTPNTITDATRLREHLKEIAAAGYAIDNEELEIGVRGVAAPIRDYRTETVGAVIISGPTMRFSDERVAA
ELVPLAQKAAETISVKLGYSGAAAE

Sequences:

>Translated_265_residues
MQKKDKSDYMILAVSHALDLLEQFHGEGVSELGVTELAKRLRLHKNNVFRLLATLEARGYVEQNRVTGNYRLGLKTLEIR
QTMIRQMALLPYAKPVLEELVRECDETAYVSILKENLSIYLYGVESRATVRVVSRLGSRLPAYCTAAGKVMLASLSEPKL
ERYLARKELVPFTPNTITDATRLREHLKEIAAAGYAIDNEELEIGVRGVAAPIRDYRTETVGAVIISGPTMRFSDERVAA
ELVPLAQKAAETISVKLGYSGAAAE
>Mature_265_residues
MQKKDKSDYMILAVSHALDLLEQFHGEGVSELGVTELAKRLRLHKNNVFRLLATLEARGYVEQNRVTGNYRLGLKTLEIR
QTMIRQMALLPYAKPVLEELVRECDETAYVSILKENLSIYLYGVESRATVRVVSRLGSRLPAYCTAAGKVMLASLSEPKL
ERYLARKELVPFTPNTITDATRLREHLKEIAAAGYAIDNEELEIGVRGVAAPIRDYRTETVGAVIISGPTMRFSDERVAA
ELVPLAQKAAETISVKLGYSGAAAE

Specific function: Unknown

COG id: COG1414

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 iclR-ED (iclR effector binding) domain [H]

Homologues:

Organism=Escherichia coli, GI1786468, Length=250, Percent_Identity=34.8, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1788131, Length=229, Percent_Identity=35.8078602620087, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI87082359, Length=250, Percent_Identity=30.8, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786715, Length=225, Percent_Identity=31.5555555555556, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1789999, Length=221, Percent_Identity=32.1266968325792, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790752, Length=225, Percent_Identity=28.8888888888889, Blast_Score=91, Evalue=6e-20,
Organism=Escherichia coli, GI1788581, Length=168, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014757
- InterPro:   IPR005471
- InterPro:   IPR011991 [H]

Pfam domain/function: PF09339 HTH_IclR; PF01614 IclR [H]

EC number: NA

Molecular weight: Translated: 29438; Mature: 29438

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS51077 HTH_ICLR ; PS51078 ICLR_ED

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKDKSDYMILAVSHALDLLEQFHGEGVSELGVTELAKRLRLHKNNVFRLLATLEARGY
CCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEQNRVTGNYRLGLKTLEIRQTMIRQMALLPYAKPVLEELVRECDETAYVSILKENLSIY
HHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCCEEE
LYGVESRATVRVVSRLGSRLPAYCTAAGKVMLASLSEPKLERYLARKELVPFTPNTITDA
EEEECCCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCHHHH
TRLREHLKEIAAAGYAIDNEELEIGVRGVAAPIRDYRTETVGAVIISGPTMRFSDERVAA
HHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHCEEEEECCCEEECCHHHHH
ELVPLAQKAAETISVKLGYSGAAAE
HHHHHHHHHHHHEEEEECCCCCCCC
>Mature Secondary Structure
MQKKDKSDYMILAVSHALDLLEQFHGEGVSELGVTELAKRLRLHKNNVFRLLATLEARGY
CCCCCCCCEEEEEHHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
VEQNRVTGNYRLGLKTLEIRQTMIRQMALLPYAKPVLEELVRECDETAYVSILKENLSIY
HHHCCCCCEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHCCCEEE
LYGVESRATVRVVSRLGSRLPAYCTAAGKVMLASLSEPKLERYLARKELVPFTPNTITDA
EEEECCCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCHHHH
TRLREHLKEIAAAGYAIDNEELEIGVRGVAAPIRDYRTETVGAVIISGPTMRFSDERVAA
HHHHHHHHHHHHHCCCCCCCCCEECHHHHHHHHHHHHHHHHCEEEEECCCEEECCHHHHH
ELVPLAQKAAETISVKLGYSGAAAE
HHHHHHHHHHHHEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]