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The map label for this gene is cooH [H]

Identifier: 78221592

GI number: 78221592

Start: 414056

End: 415564

Strand: Reverse

Name: cooH [H]

Synonym: Gmet_0371

Alternate gene names: 78221592

Gene position: 415564-414056 (Counterclockwise)

Preceding gene: 78221593

Following gene: 78221591

Centisome position: 10.4

GC content: 66.8

Gene sequence:

>1509_bases
GTGACCCGCACCTTCTCCCTCACCTGCCTGCGCAACGGAGAGGCGGTGCCGGTGGCCTCGGTGCCGCTCCTTCCCCTCGA
ACAGTTCCTCCAGGATATTATCGACGCCACCGACCGTGGATGGCGGGTCGCCTCCTGTTTCGGGGTCCCCACCGACGCCG
GCGCCACCCTCTGGTGCCTCATGGCCGCCCCCAACGACGGGCTTCTGGGGCTCATGCGAACCGAGGCGGGAATGGCCTTT
CCCTCCATCTGCCGTCTCGTCCCCCAACTCCACCTTTTCGAGCGGGAGATCGCCGAACAGTGCGGCATCACCCCCGAGGC
CCACCCCTGGCCCAAGCCGGTCCGGTTCCACCACCCCTTCATGCCGTCACGTAAAGGTTTAGGCGGGAAAGTGGCGGGTG
ACACCGACTACTACCGGGTGGAGGGGGAGGAGATCCACGAGGTGGCGGTGGGACCGGTCCACGCAGGGATCATCGAGCCG
GGGCATTTCCGCTTCCAGTGCCACGGCGAAGAGGTCTTCAGCCTGGAGATTGCCCTCGGCTACCAGCACCGGGGGGTTGA
GGAGGGCATCCTGAAGGGCCCCCACCCGAAAACCCTCTTCCAGATGGAGACCATCGCCGGCGACACCACCATCGGCCACA
CCCTGGCCTATGCCATGGTCATGGAGGCGCTCGGGGGGACGGTTCCGTCGGCCCGGGCCGAGATCATCCGGGCCATCTCC
CTGGAACTGGAACGGCTCGCCAACCACACCGGCGACCTGGGGGCACTGGCCGGGGATGTGGCCTATCTTCCCACCGCCTC
CTTCTGCGGCCGGATCAGGGGGGATTTCCTCAACATGACGGCCCATATCTGTGGAAGCCGTTTCGGCAGGGGTATCGTAC
GCCCCGGCGGGGTCGGTTTCGACGTGGGCGAACGGCAGGCGGGCGAGCTCCTCGATCGGCTCGCCGCCGCGAGCGACGAT
CTGGAAAATGCGGTGGGGCTCCTCTGGGACACCCAGTCGGTCATGGCCCGCTTCGAGGGGACAGGAAAGGTGAGTCAGGA
GACGGCCCGGGAGATCGGCCTCGTGGGTCCGGCGGCCCGGGCATGCGGTATCGACCGGGACGTGCGGCGGGATACCCCCT
TCGGCATCTACGGCGTGAGTCCGGTGGCCGTGGCTGTTGAGGAAAACGGTGACGTCTTTGCCCGGGCCCATGTCCGCTGG
CGCGAGGCGGAGGCCTCCCTCCACTTCGTAGCGGAGCAACTCCGCCAGTTGCCGGCCGGCCCTCCTGTGACGAATACCGC
CACCCCTGCGCCGGACAGCGTGGCCGTGGCTCTCACCGAGGGGTGGCGGGGTGAGATCTGCCACGTTGCCATCACCGACG
GCACCGGCCGTTTTGCCCGCTACAAGGTGGTCGACCCGTCGTTCCACAACTGGACCGGACTCGCCCTGGCCCTGCGGAAT
CAGCAGATTTCCGACTTCCCCCTCTGCAACAAGAGTTTTAATCTCTCCTACTGCGGGTTCGATTTGTGA

Upstream 100 bases:

>100_bases
TCATTCTTATGGGAGTCGTCCTCATGCTCGGTATCTGGATACCGGAACCACTCAGCCGCCTCATTACGGAAGGAGCGGCC
CTATTAGGAGGAAACGTGCC

Downstream 100 bases:

>100_bases
TATAAGAGACGGGTTTGCTTTTTCACTCCACCCGACAGAGGCTCACCGTGCAGAAAAAAGACAAATCCGATTACATGATT
CTTGCCGTCTCCCACGCCCT

Product: NADH-ubiquinone oxidoreductase, chain 49kDa

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: NA

Number of amino acids: Translated: 502; Mature: 501

Protein sequence:

>502_residues
MTRTFSLTCLRNGEAVPVASVPLLPLEQFLQDIIDATDRGWRVASCFGVPTDAGATLWCLMAAPNDGLLGLMRTEAGMAF
PSICRLVPQLHLFEREIAEQCGITPEAHPWPKPVRFHHPFMPSRKGLGGKVAGDTDYYRVEGEEIHEVAVGPVHAGIIEP
GHFRFQCHGEEVFSLEIALGYQHRGVEEGILKGPHPKTLFQMETIAGDTTIGHTLAYAMVMEALGGTVPSARAEIIRAIS
LELERLANHTGDLGALAGDVAYLPTASFCGRIRGDFLNMTAHICGSRFGRGIVRPGGVGFDVGERQAGELLDRLAAASDD
LENAVGLLWDTQSVMARFEGTGKVSQETAREIGLVGPAARACGIDRDVRRDTPFGIYGVSPVAVAVEENGDVFARAHVRW
REAEASLHFVAEQLRQLPAGPPVTNTATPAPDSVAVALTEGWRGEICHVAITDGTGRFARYKVVDPSFHNWTGLALALRN
QQISDFPLCNKSFNLSYCGFDL

Sequences:

>Translated_502_residues
MTRTFSLTCLRNGEAVPVASVPLLPLEQFLQDIIDATDRGWRVASCFGVPTDAGATLWCLMAAPNDGLLGLMRTEAGMAF
PSICRLVPQLHLFEREIAEQCGITPEAHPWPKPVRFHHPFMPSRKGLGGKVAGDTDYYRVEGEEIHEVAVGPVHAGIIEP
GHFRFQCHGEEVFSLEIALGYQHRGVEEGILKGPHPKTLFQMETIAGDTTIGHTLAYAMVMEALGGTVPSARAEIIRAIS
LELERLANHTGDLGALAGDVAYLPTASFCGRIRGDFLNMTAHICGSRFGRGIVRPGGVGFDVGERQAGELLDRLAAASDD
LENAVGLLWDTQSVMARFEGTGKVSQETAREIGLVGPAARACGIDRDVRRDTPFGIYGVSPVAVAVEENGDVFARAHVRW
REAEASLHFVAEQLRQLPAGPPVTNTATPAPDSVAVALTEGWRGEICHVAITDGTGRFARYKVVDPSFHNWTGLALALRN
QQISDFPLCNKSFNLSYCGFDL
>Mature_501_residues
TRTFSLTCLRNGEAVPVASVPLLPLEQFLQDIIDATDRGWRVASCFGVPTDAGATLWCLMAAPNDGLLGLMRTEAGMAFP
SICRLVPQLHLFEREIAEQCGITPEAHPWPKPVRFHHPFMPSRKGLGGKVAGDTDYYRVEGEEIHEVAVGPVHAGIIEPG
HFRFQCHGEEVFSLEIALGYQHRGVEEGILKGPHPKTLFQMETIAGDTTIGHTLAYAMVMEALGGTVPSARAEIIRAISL
ELERLANHTGDLGALAGDVAYLPTASFCGRIRGDFLNMTAHICGSRFGRGIVRPGGVGFDVGERQAGELLDRLAAASDDL
ENAVGLLWDTQSVMARFEGTGKVSQETAREIGLVGPAARACGIDRDVRRDTPFGIYGVSPVAVAVEENGDVFARAHVRWR
EAEASLHFVAEQLRQLPAGPPVTNTATPAPDSVAVALTEGWRGEICHVAITDGTGRFARYKVVDPSFHNWTGLALALRNQ
QISDFPLCNKSFNLSYCGFDL

Specific function: The carbon monoxide dehydrogenase (CODH) oxidizes carbon monoxide coupled, via CooF, to the reduction of a hydrogen cation by a hydrogenase (probably CooH) [H]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli formate hydrogenlyase hydrogenase isozyme 3 and to bovine mitochondrial NADH-ubiquinone oxidoreductase [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=371, Percent_Identity=23.1805929919137, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI260898743, Length=371, Percent_Identity=23.1805929919137, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1789076, Length=440, Percent_Identity=29.0909090909091, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788832, Length=439, Percent_Identity=28.7015945330296, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI145693162, Length=519, Percent_Identity=23.5067437379576, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17555284, Length=348, Percent_Identity=24.4252873563218, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17568379, Length=270, Percent_Identity=25.1851851851852, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24638644, Length=362, Percent_Identity=24.0331491712707, Blast_Score=105, Evalue=5e-23,
Organism=Drosophila melanogaster, GI221459469, Length=273, Percent_Identity=25.2747252747253, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001501 [H]

Pfam domain/function: PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 54286; Mature: 54155

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTFSLTCLRNGEAVPVASVPLLPLEQFLQDIIDATDRGWRVASCFGVPTDAGATLWCL
CCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEE
MAAPNDGLLGLMRTEAGMAFPSICRLVPQLHLFEREIAEQCGITPEAHPWPKPVRFHHPF
EECCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
MPSRKGLGGKVAGDTDYYRVEGEEIHEVAVGPVHAGIIEPGHFRFQCHGEEVFSLEIALG
CCCCCCCCCCCCCCCCEEEECCCCEEHEECCCEEECCCCCCCEEEEECCCEEEEEEEEEC
YQHRGVEEGILKGPHPKTLFQMETIAGDTTIGHTLAYAMVMEALGGTVPSARAEIIRAIS
CCCCCCHHHCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LELERLANHTGDLGALAGDVAYLPTASFCGRIRGDFLNMTAHICGSRFGRGIVRPGGVGF
HHHHHHHCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DVGERQAGELLDRLAAASDDLENAVGLLWDTQSVMARFEGTGKVSQETAREIGLVGPAAR
CCCCHHHHHHHHHHHHCCCCHHHHHHEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCHHH
ACGIDRDVRRDTPFGIYGVSPVAVAVEENGDVFARAHVRWREAEASLHFVAEQLRQLPAG
HCCCCCHHCCCCCCCEEECCEEEEEEECCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCC
PPVTNTATPAPDSVAVALTEGWRGEICHVAITDGTGRFARYKVVDPSFHNWTGLALALRN
CCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEC
QQISDFPLCNKSFNLSYCGFDL
CCCCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure 
TRTFSLTCLRNGEAVPVASVPLLPLEQFLQDIIDATDRGWRVASCFGVPTDAGATLWCL
CCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEEEE
MAAPNDGLLGLMRTEAGMAFPSICRLVPQLHLFEREIAEQCGITPEAHPWPKPVRFHHPF
EECCCCCCEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
MPSRKGLGGKVAGDTDYYRVEGEEIHEVAVGPVHAGIIEPGHFRFQCHGEEVFSLEIALG
CCCCCCCCCCCCCCCCEEEECCCCEEHEECCCEEECCCCCCCEEEEECCCEEEEEEEEEC
YQHRGVEEGILKGPHPKTLFQMETIAGDTTIGHTLAYAMVMEALGGTVPSARAEIIRAIS
CCCCCCHHHCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LELERLANHTGDLGALAGDVAYLPTASFCGRIRGDFLNMTAHICGSRFGRGIVRPGGVGF
HHHHHHHCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
DVGERQAGELLDRLAAASDDLENAVGLLWDTQSVMARFEGTGKVSQETAREIGLVGPAAR
CCCCHHHHHHHHHHHHCCCCHHHHHHEEECCHHHHHHHCCCCCCHHHHHHHHCCCCCHHH
ACGIDRDVRRDTPFGIYGVSPVAVAVEENGDVFARAHVRWREAEASLHFVAEQLRQLPAG
HCCCCCHHCCCCCCCEEECCEEEEEEECCCCEEEEEEEEEHHHHHHHHHHHHHHHHCCCC
PPVTNTATPAPDSVAVALTEGWRGEICHVAITDGTGRFARYKVVDPSFHNWTGLALALRN
CCCCCCCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEEEEEEC
QQISDFPLCNKSFNLSYCGFDL
CCCCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626276; 1644755 [H]