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The map label for this gene is hyfI [H]

Identifier: 78221590

GI number: 78221590

Start: 412399

End: 413187

Strand: Reverse

Name: hyfI [H]

Synonym: Gmet_0369

Alternate gene names: 78221590

Gene position: 413187-412399 (Counterclockwise)

Preceding gene: 78221591

Following gene: 78221583

Centisome position: 10.34

GC content: 67.43

Gene sequence:

>789_bases
ATGCTCAAGGCACTCTTTGCCAGACGTCACCAGGGACACCGAACCATCGCCTACCCCGCTGGGCCGGCGCAACTGCCGGA
ACTCTTCCGGGGGGCCCCCTTCCTGGATCCGGAAATGTGTCCCGGCGGGTGCGCCGACTGTGCCAACGCCTGTCCTCATG
GCGCCATAACGGCCGGGACCGGGGACCAGGGACCGGGGACCGGGAAAACCCTCGCCCTCGACATGGGGAAATGCCTCTTC
TGCCCTGAATGCGCCGGCGCCTGCCCCCACGGCGCCATCTCTTTCACTGGCGATCACCGGCTGGCCGTTAACCGGCGGGA
GGACCTCATCGTCCGGTCCGGAGAGGTCCGGAGGCTCGCCCCCCCCCTGGACGCGACGCGACAATTCCTCTTCAGCCACT
CCCTCAAGCTCCGCGAGGTCTCGGCAGGGGGGTGCAACGCCTGCGAGGCCGACACCAACGTCCTCTCCACCGTCGGCTTT
GACCTGGGGCGCTTCGGCATCCAGTTCGTTGCCTCCCCCCGCCATGCCGACGGCATCTTCATCACCGGCCCGGTTACCGA
GAACATGCACCTCGCCCTCCTCAAGACCTGGGAGGCGATCCCCGAGCCGAAAATCGCCATCGCCGCCGGGGCCTGCGCCA
TCAACGGTGGTCCCTTCATCGACTCCCCCGATGTTCACAACGGCGCCGACGGCCTCATCCCCATCGACCTCTACATCCCC
GGCTGTCCACCCCACCCCCTCACCATCCTCGACGGTCTCCTGCGGATCTTGGGGAGGCTGGAGGAGTAG

Upstream 100 bases:

>100_bases
AACTGGTCCCCCTGGCCCAGAAAGCCGCCGAAACCATCTCCGTAAAACTCGGCTATAGCGGGGCCGCAGCGGAATAGGTC
CCCCCCAGGGGAGGCGCACC

Downstream 100 bases:

>100_bases
CATCTATCCGGCTGCCAGCATAAAAAAACGCGACGGGAAAGCCCGCCGCGTTCAAGTATTCGAGTCTTTTCCCATATTTT
TGCCCCTGCCTACCCCTTCC

Product: NADH ubiquinone oxidoreductase, 20 kDa subunit

Products: H2; oxidized ferredoxin [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MLKALFARRHQGHRTIAYPAGPAQLPELFRGAPFLDPEMCPGGCADCANACPHGAITAGTGDQGPGTGKTLALDMGKCLF
CPECAGACPHGAISFTGDHRLAVNRREDLIVRSGEVRRLAPPLDATRQFLFSHSLKLREVSAGGCNACEADTNVLSTVGF
DLGRFGIQFVASPRHADGIFITGPVTENMHLALLKTWEAIPEPKIAIAAGACAINGGPFIDSPDVHNGADGLIPIDLYIP
GCPPHPLTILDGLLRILGRLEE

Sequences:

>Translated_262_residues
MLKALFARRHQGHRTIAYPAGPAQLPELFRGAPFLDPEMCPGGCADCANACPHGAITAGTGDQGPGTGKTLALDMGKCLF
CPECAGACPHGAISFTGDHRLAVNRREDLIVRSGEVRRLAPPLDATRQFLFSHSLKLREVSAGGCNACEADTNVLSTVGF
DLGRFGIQFVASPRHADGIFITGPVTENMHLALLKTWEAIPEPKIAIAAGACAINGGPFIDSPDVHNGADGLIPIDLYIP
GCPPHPLTILDGLLRILGRLEE
>Mature_262_residues
MLKALFARRHQGHRTIAYPAGPAQLPELFRGAPFLDPEMCPGGCADCANACPHGAITAGTGDQGPGTGKTLALDMGKCLF
CPECAGACPHGAISFTGDHRLAVNRREDLIVRSGEVRRLAPPLDATRQFLFSHSLKLREVSAGGCNACEADTNVLSTVGF
DLGRFGIQFVASPRHADGIFITGPVTENMHLALLKTWEAIPEPKIAIAAGACAINGGPFIDSPDVHNGADGLIPIDLYIP
GCPPHPLTILDGLLRILGRLEE

Specific function: Unknown

COG id: COG3260

COG function: function code C; Ni,Fe-hydrogenase III small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI187281616, Length=119, Percent_Identity=35.2941176470588, Blast_Score=95, Evalue=5e-20,
Organism=Escherichia coli, GI1788834, Length=147, Percent_Identity=41.4965986394558, Blast_Score=115, Evalue=2e-27,
Organism=Escherichia coli, GI1789074, Length=132, Percent_Identity=44.6969696969697, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1788624, Length=112, Percent_Identity=36.6071428571429, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789075, Length=103, Percent_Identity=37.8640776699029, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17509685, Length=119, Percent_Identity=36.9747899159664, Blast_Score=95, Evalue=4e-20,
Organism=Drosophila melanogaster, GI18859983, Length=119, Percent_Identity=36.1344537815126, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24642371, Length=119, Percent_Identity=36.1344537815126, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI24651058, Length=119, Percent_Identity=33.6134453781513, Blast_Score=92, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 27615; Mature: 27615

Theoretical pI: Translated: 6.49; Mature: 6.49

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKALFARRHQGHRTIAYPAGPAQLPELFRGAPFLDPEMCPGGCADCANACPHGAITAGT
CCHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCEECCC
GDQGPGTGKTLALDMGKCLFCPECAGACPHGAISFTGDHRLAVNRREDLIVRSGEVRRLA
CCCCCCCCCEEEEECCCEEECHHHCCCCCCCCEEECCCCEEEECCCCCEEEECCCCEECC
PPLDATRQFLFSHSLKLREVSAGGCNACEADTNVLSTVGFDLGRFGIQFVASPRHADGIF
CCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCEE
ITGPVTENMHLALLKTWEAIPEPKIAIAAGACAINGGPFIDSPDVHNGADGLIPIDLYIP
EECCCCCCEEEEEEHHHHHCCCCCEEEEECEEEECCCCCCCCCCCCCCCCCEEEEEEEEC
GCPPHPLTILDGLLRILGRLEE
CCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKALFARRHQGHRTIAYPAGPAQLPELFRGAPFLDPEMCPGGCADCANACPHGAITAGT
CCHHHHHHHHCCCEEEEECCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHCCCCCCEECCC
GDQGPGTGKTLALDMGKCLFCPECAGACPHGAISFTGDHRLAVNRREDLIVRSGEVRRLA
CCCCCCCCCEEEEECCCEEECHHHCCCCCCCCEEECCCCEEEECCCCCEEEECCCCEECC
PPLDATRQFLFSHSLKLREVSAGGCNACEADTNVLSTVGFDLGRFGIQFVASPRHADGIF
CCHHHHHHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCEEEEECCCCCCCEE
ITGPVTENMHLALLKTWEAIPEPKIAIAAGACAINGGPFIDSPDVHNGADGLIPIDLYIP
EECCCCCCEEEEEEHHHHHCCCCCEEEEECEEEECCCCCCCCCCCCCCCCCEEEEEEEEC
GCPPHPLTILDGLLRILGRLEE
CCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton; reduced ferredoxin [C]

Specific reaction: Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]