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The map label for this gene is hyfR [H]

Identifier: 78221560

GI number: 78221560

Start: 378250

End: 379629

Strand: Direct

Name: hyfR [H]

Synonym: Gmet_0339

Alternate gene names: 78221560

Gene position: 378250-379629 (Clockwise)

Preceding gene: 78221559

Following gene: 78221561

Centisome position: 9.46

GC content: 61.59

Gene sequence:

>1380_bases
ATGGGGAGTGAAAAAGGAACTATTGATCCCGCTGTGTACCGGGCGATTCTGGCAAGTATGGGGGATGGAATCGTCTTCCT
GGATACGGAGGACTGTTTCGCCTACATCAACGCGGCCGCAGAGAAGATCCGCGGCATCCGGGCGGAAAACTACCTGGGGC
GCCATATCCTTTCCATCTTCGGCACCCATTCCAACCATACGTCCGAGCGGGTGCGCAAGCTGCTGGCCGCCCTCAGGAGC
GGCGTCCTCCCCTTTCATGTCCATATCATCGAGACGAGGGGGAGGGTTTTCGAGAACAGCTATTACCCCATCCGCGATAA
GGGAGGCAACTATCTCGGAACCCTGATGATGAGCCGCGACATCACCGAGAAGGAGCGGCTCAAGGCGGAAAACCTCACCC
TTCGCGAGCAGGTGCTGTCCGAATTCGGCTGCTGCGACCTGGTGGGCTCCAGCGATGTCATGCGCCCCATCTTCCAGATC
ATCAATGCCGCCGCTGCCGTCGACTCCACCATCCTGATCACCGGCGAAAGCGGCACCGGCAAGGAGCTGGTGGCCCGGGC
CGTCCACCAGCACAGCGCGCGCAAGGATCTCCCCATGATCAAGGTCAACTGCGCGGCCCTCCCCGATACCCTCCTGGAAT
CGGAACTCTTCGGCCATGAAAAGGGGGCGTTTACCGGTGCCATCAAGGAGCGCAAGGGGAAATTCGAGCAGGCCCACGGG
GGAACCATCTTCCTCGATGAGATCGCGGAGATGCCCCTGGCGGCCCAGGCCAAACTGCTCAGAGTGCTGCAGGAGAGGAC
CGTGGAGCGCCTGGGGAGCACCCGGGAGATCAGGGTGGACGTGCGGATCATCGCCGCCACCAACCGCGACCTGCGCCGGG
AGGTGACGGCGGGACGGTTTCGGGAAGACCTCTTCTATCGCCTCAACGTCATCCCGATCCATATCCCCCCCCTGCGGGAG
CGGACCGAGGATATCCTCGCCCTGGCCCGGCAGTTCCTCAACCGGTTCGCCGAGAAGATGCACAAGCCGGTCATGGAAAT
CGCCCCGGAGGCCCAGAAGGCCCTCCTGGAATACAACTATCCGGGCAATGTCAGGGAACTGGAAAATGCTGTCGAGCGGG
CCGTTGCCCTCTCCCTTGGCAGCAATCTGCGCCTCGGAGATCTCCCGTCGGAGTTCACCGCTCCGCACCCAGCCGGTGCT
CAGCCGCTTTCCCCGTCCGCCGGTCCCGCTTCCCTGGCCGATAGTGTTCATTCCATGGAAAAAAACGCTATCGAAGAGGC
CTTGCGCTCCACTGGCAACAAGAAGGGAGATGCCGCTCGACTCCTCGGTATCTCCCGGAAAACCTTGTGGGAAAAAATGA
AACAGCTGGGGGTCACGTAG

Upstream 100 bases:

>100_bases
ATACTACCTGGTCGCCCGAAGCCAGCAGATATCGCCCATACGCTGAGTGGGGAGCGGGACTTATGCTACTCTTTGAAATA
CAATGACGTGAGGGTGAGCC

Downstream 100 bases:

>100_bases
CGGAAGCAGATGGTATGGGTTCCGGAGGCTTCCCTGGCATCGGGAAATATTTTTCCCCTGTGAAAATTATTCGTGGACTT
TTGGGTGGAAAAGCTGGTAG

Product: sigma-54 dependent trancsriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MGSEKGTIDPAVYRAILASMGDGIVFLDTEDCFAYINAAAEKIRGIRAENYLGRHILSIFGTHSNHTSERVRKLLAALRS
GVLPFHVHIIETRGRVFENSYYPIRDKGGNYLGTLMMSRDITEKERLKAENLTLREQVLSEFGCCDLVGSSDVMRPIFQI
INAAAAVDSTILITGESGTGKELVARAVHQHSARKDLPMIKVNCAALPDTLLESELFGHEKGAFTGAIKERKGKFEQAHG
GTIFLDEIAEMPLAAQAKLLRVLQERTVERLGSTREIRVDVRIIAATNRDLRREVTAGRFREDLFYRLNVIPIHIPPLRE
RTEDILALARQFLNRFAEKMHKPVMEIAPEAQKALLEYNYPGNVRELENAVERAVALSLGSNLRLGDLPSEFTAPHPAGA
QPLSPSAGPASLADSVHSMEKNAIEEALRSTGNKKGDAARLLGISRKTLWEKMKQLGVT

Sequences:

>Translated_459_residues
MGSEKGTIDPAVYRAILASMGDGIVFLDTEDCFAYINAAAEKIRGIRAENYLGRHILSIFGTHSNHTSERVRKLLAALRS
GVLPFHVHIIETRGRVFENSYYPIRDKGGNYLGTLMMSRDITEKERLKAENLTLREQVLSEFGCCDLVGSSDVMRPIFQI
INAAAAVDSTILITGESGTGKELVARAVHQHSARKDLPMIKVNCAALPDTLLESELFGHEKGAFTGAIKERKGKFEQAHG
GTIFLDEIAEMPLAAQAKLLRVLQERTVERLGSTREIRVDVRIIAATNRDLRREVTAGRFREDLFYRLNVIPIHIPPLRE
RTEDILALARQFLNRFAEKMHKPVMEIAPEAQKALLEYNYPGNVRELENAVERAVALSLGSNLRLGDLPSEFTAPHPAGA
QPLSPSAGPASLADSVHSMEKNAIEEALRSTGNKKGDAARLLGISRKTLWEKMKQLGVT
>Mature_458_residues
GSEKGTIDPAVYRAILASMGDGIVFLDTEDCFAYINAAAEKIRGIRAENYLGRHILSIFGTHSNHTSERVRKLLAALRSG
VLPFHVHIIETRGRVFENSYYPIRDKGGNYLGTLMMSRDITEKERLKAENLTLREQVLSEFGCCDLVGSSDVMRPIFQII
NAAAAVDSTILITGESGTGKELVARAVHQHSARKDLPMIKVNCAALPDTLLESELFGHEKGAFTGAIKERKGKFEQAHGG
TIFLDEIAEMPLAAQAKLLRVLQERTVERLGSTREIRVDVRIIAATNRDLRREVTAGRFREDLFYRLNVIPIHIPPLRER
TEDILALARQFLNRFAEKMHKPVMEIAPEAQKALLEYNYPGNVRELENAVERAVALSLGSNLRLGDLPSEFTAPHPAGAQ
PLSPSAGPASLADSVHSMEKNAIEEALRSTGNKKGDAARLLGISRKTLWEKMKQLGVT

Specific function: Required for induction of expression of the hydrogenase- 4 structural genes [H]

COG id: COG3604

COG function: function code KT; Transcriptional regulator containing GAF, AAA-type ATPase, and DNA binding domains

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=370, Percent_Identity=47.2972972972973, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1790437, Length=318, Percent_Identity=51.8867924528302, Blast_Score=304, Evalue=1e-83,
Organism=Escherichia coli, GI1788550, Length=334, Percent_Identity=46.4071856287425, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1789087, Length=339, Percent_Identity=48.6725663716814, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1790299, Length=332, Percent_Identity=46.6867469879518, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=45.5108359133127, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789233, Length=451, Percent_Identity=36.5853658536585, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI1788905, Length=306, Percent_Identity=44.7712418300654, Blast_Score=241, Evalue=8e-65,
Organism=Escherichia coli, GI1786524, Length=333, Percent_Identity=41.1411411411411, Blast_Score=228, Evalue=8e-61,
Organism=Escherichia coli, GI87081872, Length=306, Percent_Identity=40.1960784313725, Blast_Score=214, Evalue=9e-57,
Organism=Escherichia coli, GI1787583, Length=314, Percent_Identity=38.2165605095541, Blast_Score=212, Evalue=5e-56,
Organism=Escherichia coli, GI87081858, Length=464, Percent_Identity=29.5258620689655, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1789828, Length=244, Percent_Identity=38.1147540983607, Blast_Score=142, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50780; Mature: 50649

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSEKGTIDPAVYRAILASMGDGIVFLDTEDCFAYINAAAEKIRGIRAENYLGRHILSIF
CCCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GTHSNHTSERVRKLLAALRSGVLPFHVHIIETRGRVFENSYYPIRDKGGNYLGTLMMSRD
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHCCCCCCCCCCCCCEEEHHHHHCC
ITEKERLKAENLTLREQVLSEFGCCDLVGSSDVMRPIFQIINAAAAVDSTILITGESGTG
CCHHHHHHHHCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
KELVARAVHQHSARKDLPMIKVNCAALPDTLLESELFGHEKGAFTGAIKERKGKFEQAHG
HHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCHHCCC
GTIFLDEIAEMPLAAQAKLLRVLQERTVERLGSTREIRVDVRIIAATNRDLRREVTAGRF
CEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCHHHHHHHHHHHH
REDLFYRLNVIPIHIPPLRERTEDILALARQFLNRFAEKMHKPVMEIAPEAQKALLEYNY
HHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHCCC
PGNVRELENAVERAVALSLGSNLRLGDLPSEFTAPHPAGAQPLSPSAGPASLADSVHSME
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KNAIEEALRSTGNKKGDAARLLGISRKTLWEKMKQLGVT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GSEKGTIDPAVYRAILASMGDGIVFLDTEDCFAYINAAAEKIRGIRAENYLGRHILSIF
CCCCCCCCHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
GTHSNHTSERVRKLLAALRSGVLPFHVHIIETRGRVFENSYYPIRDKGGNYLGTLMMSRD
CCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHCCCCCCCCCCCCCEEEHHHHHCC
ITEKERLKAENLTLREQVLSEFGCCDLVGSSDVMRPIFQIINAAAAVDSTILITGESGTG
CCHHHHHHHHCCHHHHHHHHHCCCEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCC
KELVARAVHQHSARKDLPMIKVNCAALPDTLLESELFGHEKGAFTGAIKERKGKFEQAHG
HHHHHHHHHHHHHHCCCCEEEEEHHHCCHHHHHHHHHCCCCCCHHHHHHHHCCCCHHCCC
GTIFLDEIAEMPLAAQAKLLRVLQERTVERLGSTREIRVDVRIIAATNRDLRREVTAGRF
CEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECCHHHHHHHHHHHH
REDLFYRLNVIPIHIPPLRERTEDILALARQFLNRFAEKMHKPVMEIAPEAQKALLEYNY
HHHHHHEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHHHHCCC
PGNVRELENAVERAVALSLGSNLRLGDLPSEFTAPHPAGAQPLSPSAGPASLADSVHSME
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KNAIEEALRSTGNKKGDAARLLGISRKTLWEKMKQLGVT
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]