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The map label for this gene is moaA [H]

Identifier: 78221558

GI number: 78221558

Start: 376183

End: 377163

Strand: Direct

Name: moaA [H]

Synonym: Gmet_0337

Alternate gene names: 78221558

Gene position: 376183-377163 (Clockwise)

Preceding gene: 78221557

Following gene: 78221559

Centisome position: 9.41

GC content: 65.95

Gene sequence:

>981_bases
ATGGAACTGATCGATACCTACGGACGGCGAATCACTTACCTGCGCCTCTCGGTGACCGACCGCTGCAATCTCCGCTGCCG
TTACTGCATGCCGGCCGCGGGGGTGGAGAAGCTCGGCCACGGCGACATCCTGAGCTACGAGGATCTCTTTCGGATCGCCC
GGGCCGCCGTTGCCGTGGGGATCGAGAAGATCCGGGTGACGGGCGGGGAGCCTCTGGTGCGGAAGGGGATCGTCCCGTTC
CTTGCCCGGCTCGCAAAACTCCCGGGGCTTCGGGAACTGGTCCTCACCACCAACGGGTTCCTCCTGCCGGAGATGGCGGG
GGAACTGCGCCGGGCCGGGGTGCAGCGGCTCAACGTGAGCCTCGATTCGTTCCGTCCCGGCACGTTCCGGGCCATCACAC
GAGGTGGCGACGTGTGGCAGGTGCTTGCCGGGATCGCCGCCGCGGAGGCGGCCGGATTTCCGCCCCCCAAGATCAACATG
GTGGTAATGCGGGGGGTGAATGATGACGAGGTGCTTGACTTCGCGCGGCTCACCATCGAGCATCCCTACGCGGTCCGCTT
CATCGAGTCCATGCCGGCCACGAGGGAGAAGAACTGGCAGAATCTGACCATTGCGGGGGAGGAAATCCTTGAACGGATAG
GGACGCAGTTCTCCCTGGAACCGGTGGGAAAGGGGAGCCTGGCGGGGCCGTCCCGGGATTTCCGCCTGAGGGGGGCTGCC
GGCACCATCGGAATCATCACGGCCCTCTCCGGTCACTTCTGCGCCGACTGCAACCGGATTCGGGTCACCTCCACCGGCAT
GGCAAAGTCGTGCCTCTTTTCCGACGGCGGAATCGACCTCAAGCCCTTTCTCAGGTGCGAAGGTTCGAAGCCCCTGGCCG
AGGCTCTCCGAAACCTGGCCTGCGCCAAGCCGGCGTGCCACGGCATGTCGATGGAGAGGGCCGAGCACCGGGCCTTTGCC
ATGGCGGCGGTCGGGGGGTAG

Upstream 100 bases:

>100_bases
AACGGGCTCGTGAAGCTCCCTCCCGACGCCGCCATCGTCGCTGGCAGCGAGGTGGAGGTGCAGCTCCTGGACCGGCACTT
CGAGATGGGTCACCCCTGAG

Downstream 100 bases:

>100_bases
GATCACCCCTTTCGCCCTTTCGTCACGTTTCCGTTCTATTTCCTGAATTCATTCACAAGCCGTGTATAATGAAAAATATA
GTAATCTCGGGAAACGTCAG

Product: radical SAM:molybdenum cofactor synthesis-like

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MELIDTYGRRITYLRLSVTDRCNLRCRYCMPAAGVEKLGHGDILSYEDLFRIARAAVAVGIEKIRVTGGEPLVRKGIVPF
LARLAKLPGLRELVLTTNGFLLPEMAGELRRAGVQRLNVSLDSFRPGTFRAITRGGDVWQVLAGIAAAEAAGFPPPKINM
VVMRGVNDDEVLDFARLTIEHPYAVRFIESMPATREKNWQNLTIAGEEILERIGTQFSLEPVGKGSLAGPSRDFRLRGAA
GTIGIITALSGHFCADCNRIRVTSTGMAKSCLFSDGGIDLKPFLRCEGSKPLAEALRNLACAKPACHGMSMERAEHRAFA
MAAVGG

Sequences:

>Translated_326_residues
MELIDTYGRRITYLRLSVTDRCNLRCRYCMPAAGVEKLGHGDILSYEDLFRIARAAVAVGIEKIRVTGGEPLVRKGIVPF
LARLAKLPGLRELVLTTNGFLLPEMAGELRRAGVQRLNVSLDSFRPGTFRAITRGGDVWQVLAGIAAAEAAGFPPPKINM
VVMRGVNDDEVLDFARLTIEHPYAVRFIESMPATREKNWQNLTIAGEEILERIGTQFSLEPVGKGSLAGPSRDFRLRGAA
GTIGIITALSGHFCADCNRIRVTSTGMAKSCLFSDGGIDLKPFLRCEGSKPLAEALRNLACAKPACHGMSMERAEHRAFA
MAAVGG
>Mature_326_residues
MELIDTYGRRITYLRLSVTDRCNLRCRYCMPAAGVEKLGHGDILSYEDLFRIARAAVAVGIEKIRVTGGEPLVRKGIVPF
LARLAKLPGLRELVLTTNGFLLPEMAGELRRAGVQRLNVSLDSFRPGTFRAITRGGDVWQVLAGIAAAEAAGFPPPKINM
VVMRGVNDDEVLDFARLTIEHPYAVRFIESMPATREKNWQNLTIAGEEILERIGTQFSLEPVGKGSLAGPSRDFRLRGAA
GTIGIITALSGHFCADCNRIRVTSTGMAKSCLFSDGGIDLKPFLRCEGSKPLAEALRNLACAKPACHGMSMERAEHRAFA
MAAVGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z [H]

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family [H]

Homologues:

Organism=Homo sapiens, GI116734668, Length=329, Percent_Identity=39.8176291793313, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI22027536, Length=328, Percent_Identity=39.6341463414634, Blast_Score=234, Evalue=7e-62,
Organism=Escherichia coli, GI1786999, Length=299, Percent_Identity=35.7859531772575, Blast_Score=184, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI32566331, Length=314, Percent_Identity=34.7133757961783, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI32566329, Length=313, Percent_Identity=34.8242811501597, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17561210, Length=310, Percent_Identity=32.9032258064516, Blast_Score=196, Evalue=1e-50,
Organism=Drosophila melanogaster, GI28574490, Length=289, Percent_Identity=41.522491349481, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI28574488, Length=329, Percent_Identity=38.2978723404255, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 35402; Mature: 35402

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELIDTYGRRITYLRLSVTDRCNLRCRYCMPAAGVEKLGHGDILSYEDLFRIARAAVAVG
CCCHHHHCCEEEEEEEEECCCCCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHH
IEKIRVTGGEPLVRKGIVPFLARLAKLPGLRELVLTTNGFLLPEMAGELRRAGVQRLNVS
HEEEEECCCCHHHHHCHHHHHHHHHHCCCCEEEEEECCCEECHHHHHHHHHHHHHHEECC
LDSFRPGTFRAITRGGDVWQVLAGIAAAEAAGFPPPKINMVVMRGVNDDEVLDFARLTIE
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCC
HPYAVRFIESMPATREKNWQNLTIAGEEILERIGTQFSLEPVGKGSLAGPSRDFRLRGAA
CCHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCC
GTIGIITALSGHFCADCNRIRVTSTGMAKSCLFSDGGIDLKPFLRCEGSKPLAEALRNLA
CHHHHHHHHCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCHHEEECCCCHHHHHHHHHH
CAKPACHGMSMERAEHRAFAMAAVGG
HCCHHHCCCCHHHHHHHHEEEEECCC
>Mature Secondary Structure
MELIDTYGRRITYLRLSVTDRCNLRCRYCMPAAGVEKLGHGDILSYEDLFRIARAAVAVG
CCCHHHHCCEEEEEEEEECCCCCCEEEEECCCCCHHHCCCCCCCCHHHHHHHHHHHHHHH
IEKIRVTGGEPLVRKGIVPFLARLAKLPGLRELVLTTNGFLLPEMAGELRRAGVQRLNVS
HEEEEECCCCHHHHHCHHHHHHHHHHCCCCEEEEEECCCEECHHHHHHHHHHHHHHEECC
LDSFRPGTFRAITRGGDVWQVLAGIAAAEAAGFPPPKINMVVMRGVNDDEVLDFARLTIE
HHCCCCCCEEEEECCCHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHCC
HPYAVRFIESMPATREKNWQNLTIAGEEILERIGTQFSLEPVGKGSLAGPSRDFRLRGAA
CCHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEECCC
GTIGIITALSGHFCADCNRIRVTSTGMAKSCLFSDGGIDLKPFLRCEGSKPLAEALRNLA
CHHHHHHHHCCCCCCCCCEEEEECCCCHHHHEECCCCCCCCHHEEECCCCHHHHHHHHHH
CAKPACHGMSMERAEHRAFAMAAVGG
HCCHHHCCCCHHHHHHHHEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA