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The map label for this gene is moeA [H]

Identifier: 78221557

GI number: 78221557

Start: 374973

End: 376181

Strand: Direct

Name: moeA [H]

Synonym: Gmet_0336

Alternate gene names: 78221557

Gene position: 374973-376181 (Clockwise)

Preceding gene: 78221556

Following gene: 78221558

Centisome position: 9.38

GC content: 66.58

Gene sequence:

>1209_bases
ATGATCTCCATTGAAGAAGCGCAACAGATCATCCTTTCCCACACGGGGCTTCTGGGAACCGAGGAGGTTCCCCTGTTGCA
CGCGCTTCACCGGGTGATCGCGGAGGATGTCCACGCGCCGTGGGATATCCCGGGGGCCGACCATTCGGCCATGGATGGCT
ACACCTTTGCCTACGACGACGTGCAGGGGGGAAGCCTCGAGGTCTGCGGCTTCCTTCCTGCCGGCGTGGCACCGACGGAG
CCGGTTTCCCCCGGCTGCGCCATGAAAATCATGACCGGCGCTCCGATTCCCCCCGGTTGCGATACCGTTGTTCCCGTGGA
GGATGTGCTGGAGGAAGGTACAACCATTCGCCTGCGGGGGATGGTCAAGCGGGGCGACCATATCCGCCGCCGTGGCGAGG
AGGTGAACGCCGGTGATCTGGTCGTTGCCTCCGGAAGCCTCCTGCGCCCTCCGGAGATCGGCATCCTCTCTTCCCTGGGG
AAGACGACAGCCACCGTGTTCCGCAAGGCCCGCGTCGCGGTTCTCTCCACCGGCGACGAGCTGTTGCAACCCGGCTCCAT
CCCGGAGCCGGGCAAGATCATCAACAGCAACAGTACCAGCATTGCCGCCCAGCTTCTGGAGGCGGGGGCCGAGCCGCTCA
TGCTCGGCATCGCCCGGGATGATCTGGAGACTACCCGGCGCAAGATCAGCGACGGGCTCCAGGCCGATCTCCTCGTCACC
ACCGGCGGCGTGTCGGTGGGGGACAGGGACTTTGTCCGCGATGCGCTCCTGGACCTGGGGGGAGAGATCAAATTCTGGAA
GGTGGCCATGAAGCCGGGGAAGCCGGTGGCCTTTGCCATGGTCCGGGGGAAACCGGTCTTTGCCCTGCCGGGGAACCCGG
TGGCGGCCATGGTCGCCTTCGAGCAGTTCGTCCGTCCAGCCCTCCTGACGATGATGGGGCATGCCCGCATCCTTCGGCCC
GTGGTCCGGGGAACCCTGGAGGAGGCCATCAGGAACCGGGGAGGGAGGCCCCACTTTGTACGGGTGTCGGTCCGCCTCGA
GGCGGGGCGCTACCGGCTGGCCACCACCGGCGACCAGGGGTCGGCGCGGCTTTCATCCCTCACCAGCGGCAACGGGCTCG
TGAAGCTCCCTCCCGACGCCGCCATCGTCGCTGGCAGCGAGGTGGAGGTGCAGCTCCTGGACCGGCACTTCGAGATGGGT
CACCCCTGA

Upstream 100 bases:

>100_bases
TGCCACAAGAAGTAAATATGGGAACCTGCGGTAGGGGCGCTGCTTGCCGCGCCGCCCCTACCGTTTAGCCACCGAATCGA
TGGCCTAAAGAGGTGATGCC

Downstream 100 bases:

>100_bases
GATGGAACTGATCGATACCTACGGACGGCGAATCACTTACCTGCGCCTCTCGGTGACCGACCGCTGCAATCTCCGCTGCC
GTTACTGCATGCCGGCCGCG

Product: molybdopterin biosynthesis protein MoeA

Products: NA

Alternate protein names: MPT Mo-transferase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MISIEEAQQIILSHTGLLGTEEVPLLHALHRVIAEDVHAPWDIPGADHSAMDGYTFAYDDVQGGSLEVCGFLPAGVAPTE
PVSPGCAMKIMTGAPIPPGCDTVVPVEDVLEEGTTIRLRGMVKRGDHIRRRGEEVNAGDLVVASGSLLRPPEIGILSSLG
KTTATVFRKARVAVLSTGDELLQPGSIPEPGKIINSNSTSIAAQLLEAGAEPLMLGIARDDLETTRRKISDGLQADLLVT
TGGVSVGDRDFVRDALLDLGGEIKFWKVAMKPGKPVAFAMVRGKPVFALPGNPVAAMVAFEQFVRPALLTMMGHARILRP
VVRGTLEEAIRNRGGRPHFVRVSVRLEAGRYRLATTGDQGSARLSSLTSGNGLVKLPPDAAIVAGSEVEVQLLDRHFEMG
HP

Sequences:

>Translated_402_residues
MISIEEAQQIILSHTGLLGTEEVPLLHALHRVIAEDVHAPWDIPGADHSAMDGYTFAYDDVQGGSLEVCGFLPAGVAPTE
PVSPGCAMKIMTGAPIPPGCDTVVPVEDVLEEGTTIRLRGMVKRGDHIRRRGEEVNAGDLVVASGSLLRPPEIGILSSLG
KTTATVFRKARVAVLSTGDELLQPGSIPEPGKIINSNSTSIAAQLLEAGAEPLMLGIARDDLETTRRKISDGLQADLLVT
TGGVSVGDRDFVRDALLDLGGEIKFWKVAMKPGKPVAFAMVRGKPVFALPGNPVAAMVAFEQFVRPALLTMMGHARILRP
VVRGTLEEAIRNRGGRPHFVRVSVRLEAGRYRLATTGDQGSARLSSLTSGNGLVKLPPDAAIVAGSEVEVQLLDRHFEMG
HP
>Mature_402_residues
MISIEEAQQIILSHTGLLGTEEVPLLHALHRVIAEDVHAPWDIPGADHSAMDGYTFAYDDVQGGSLEVCGFLPAGVAPTE
PVSPGCAMKIMTGAPIPPGCDTVVPVEDVLEEGTTIRLRGMVKRGDHIRRRGEEVNAGDLVVASGSLLRPPEIGILSSLG
KTTATVFRKARVAVLSTGDELLQPGSIPEPGKIINSNSTSIAAQLLEAGAEPLMLGIARDDLETTRRKISDGLQADLLVT
TGGVSVGDRDFVRDALLDLGGEIKFWKVAMKPGKPVAFAMVRGKPVFALPGNPVAAMVAFEQFVRPALLTMMGHARILRP
VVRGTLEEAIRNRGGRPHFVRVSVRLEAGRYRLATTGDQGSARLSSLTSGNGLVKLPPDAAIVAGSEVEVQLLDRHFEMG
HP

Specific function: Catalyzes the insertion of molybdate into adenylated molybdopterin with the concomitant release of AMP [H]

COG id: COG0303

COG function: function code H; Molybdopterin biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the moeA family [H]

Homologues:

Organism=Homo sapiens, GI10880983, Length=397, Percent_Identity=37.0277078085642, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI66932975, Length=397, Percent_Identity=37.0277078085642, Blast_Score=212, Evalue=6e-55,
Organism=Escherichia coli, GI1787049, Length=384, Percent_Identity=39.3229166666667, Blast_Score=236, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI25147659, Length=374, Percent_Identity=33.6898395721925, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17562606, Length=338, Percent_Identity=28.4023668639053, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17136978, Length=362, Percent_Identity=32.3204419889503, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI24638841, Length=362, Percent_Identity=32.3204419889503, Blast_Score=160, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020817
- InterPro:   IPR008284
- InterPro:   IPR005111
- InterPro:   IPR005110
- InterPro:   IPR001453 [H]

Pfam domain/function: PF00994 MoCF_biosynth; PF03454 MoeA_C; PF03453 MoeA_N [H]

EC number: NA

Molecular weight: Translated: 42700; Mature: 42700

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS01079 MOCF_BIOSYNTHESIS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISIEEAQQIILSHTGLLGTEEVPLLHALHRVIAEDVHAPWDIPGADHSAMDGYTFAYDD
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECC
VQGGSLEVCGFLPAGVAPTEPVSPGCAMKIMTGAPIPPGCDTVVPVEDVLEEGTTIRLRG
CCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCEEEEEE
MVKRGDHIRRRGEEVNAGDLVVASGSLLRPPEIGILSSLGKTTATVFRKARVAVLSTGDE
EHHCCHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHEEEEECCHH
LLQPGSIPEPGKIINSNSTSIAAQLLEAGAEPLMLGIARDDLETTRRKISDGLQADLLVT
HHCCCCCCCCCCEECCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCEEEEEE
TGGVSVGDRDFVRDALLDLGGEIKFWKVAMKPGKPVAFAMVRGKPVFALPGNPVAAMVAF
ECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCEEEEECCCCHHHHHHH
EQFVRPALLTMMGHARILRPVVRGTLEEAIRNRGGRPHFVRVSVRLEAGRYRLATTGDQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEECCCCC
SARLSSLTSGNGLVKLPPDAAIVAGSEVEVQLLDRHFEMGHP
CHHHHHHCCCCCEEECCCCCEEEECCCEEEHHHHHHHCCCCC
>Mature Secondary Structure
MISIEEAQQIILSHTGLLGTEEVPLLHALHRVIAEDVHAPWDIPGADHSAMDGYTFAYDD
CCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECC
VQGGSLEVCGFLPAGVAPTEPVSPGCAMKIMTGAPIPPGCDTVVPVEDVLEEGTTIRLRG
CCCCCEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHCCCCEEEEEE
MVKRGDHIRRRGEEVNAGDLVVASGSLLRPPEIGILSSLGKTTATVFRKARVAVLSTGDE
EHHCCHHHHHCCCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHHHHHEEEEECCHH
LLQPGSIPEPGKIINSNSTSIAAQLLEAGAEPLMLGIARDDLETTRRKISDGLQADLLVT
HHCCCCCCCCCCEECCCCHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHCCCCEEEEEE
TGGVSVGDRDFVRDALLDLGGEIKFWKVAMKPGKPVAFAMVRGKPVFALPGNPVAAMVAF
ECCCCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCEEEEEECCCEEEEECCCCHHHHHHH
EQFVRPALLTMMGHARILRPVVRGTLEEAIRNRGGRPHFVRVSVRLEAGRYRLATTGDQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCEEEEECCCCC
SARLSSLTSGNGLVKLPPDAAIVAGSEVEVQLLDRHFEMGHP
CHHHHHHCCCCCEEECCCCCEEEECCCEEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503; 9515915 [H]