Click here to switch to the map view.

The map label for this gene is narT [H]

Identifier: 78221554

GI number: 78221554

Start: 371779

End: 373098

Strand: Direct

Name: narT [H]

Synonym: Gmet_0333

Alternate gene names: 78221554

Gene position: 371779-373098 (Clockwise)

Preceding gene: 78221553

Following gene: 78221555

Centisome position: 9.3

GC content: 62.35

Gene sequence:

>1320_bases
ATGGAAAGGCACGAGCAGAAGACGGCAACGGCGGCAACGTGGGGGACCCAGGTCAGGACGCGCACCATGGCGACCCACAG
GTACAAGCAGATCTCGGTCCTGACGATGAACACCTTCGCCTTCACTGTCTGCTTCGCGGTCTGGGTGATGTTCTCCATCA
TCGGCATCCCGATCAAGGGCGCCCTAGGCCTGAACGAGACCCAGTTCGGCATCCTGGCCGCAACCCCCATTCTCTCCGGC
TCATTGATTCGGCTGCCGCTCGGGATGTGGACCGACAAGTACGGCGGACGCATTGTCTTCTTCATTCTGATGCTCTGCAC
CATCGTCCCCATCTACCTGGTGAGCGCCGCCACGGCGTACTGGCACTTTCTCCTCCTGGGGCTCTTCATCGGTCTTGCCG
GCGGCGCCTTCTCGGTGGGGATCAGCTACACCGCCCGCTGGTTCACCAAGGCCCGTCAGGGATTCGCCATGGGGATCTTT
GGCGCCGGGAACGCGGGGGCCGCGGTCACCAAGTTCCTGGCCCCCTCCCTGGTGGTGGCCTACGGCTGGCAGATGGTCCC
CAAGGTCTATGCCGTGGCCATGCTCGTGACGGCCATCCTGTTTTGGGCATTCACCTTCACCGAGCACAGCCACAAAGTGA
GCTCCACCGTGACCATCAAAGACCAGCTGGCGGCCCTCAGGGACCCGAAGGTCTGGCGCTACTGCCAGTACTACGCCATC
GTCTTCGGGGGCTACGTGGGGCTCTCCCTCTGGATGACCAAGTACTACATCAACGAGTACGGCTTCGACATCCGGACCGC
GGCCCTGGTGGCCGCCATCTTCGTGCTACCCTCCGGCGTGATCCGCGCCCTGGGAGGGTGGCTGTCGGACCGTTTCGGCG
CCCACACCGTCACCTGCTGGGTCATGTGGGTCTCCGGCGGGTGCCTCCTGCTCCTCTCGATCCCCCACTTCGATGTGGTT
GTCACCACGGTGAACGGTCCCGTCGCGCGCCACGTGGGGCTCAACGTATGGGTGTTCACCTCCCTTCTCTTCGTTGTCGG
CATTGCGTGGGGATTCGGCAAGGCATCGGTGTTCAAGCACATCTCCGATGAATACCCCCACAACATCGGCGTGATCTCCG
GCATCGTGGGGCTCATTGGCGGGCTCGGCGGGTTTGTCCTCCCCATCATGTTCGGCGCCCTGGTGGACCTGACCGGTGTC
CGGAGCACCGCTTTCATGCTCCTGTACGGTTCCACGTGCGTTTCCCTGTTCTGGATGTACCTGGCCCTGAAGAAAGAAAA
CGTTCATCGCAAGCTGAAGACCGCCGACATTCTCGAATAA

Upstream 100 bases:

>100_bases
TCTACTGGCCTCTCGATTTTCTCAAGGATCCACCCATCCTCTACCGCTGGCGCTCCCGGCCCGAGGGGAAGGTGGAGTAA
CGGCATTCAAGGAGAACGCC

Downstream 100 bases:

>100_bases
CAGCCAATAACAGCCAATTCCCATGGAAGGAGAATAACGATGCCAACGGACGTATTGACAGCAACGGAAACGAAAAAGAG
TGCATCATCGCACGTTCTGA

Product: major facilitator transporter

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MERHEQKTATAATWGTQVRTRTMATHRYKQISVLTMNTFAFTVCFAVWVMFSIIGIPIKGALGLNETQFGILAATPILSG
SLIRLPLGMWTDKYGGRIVFFILMLCTIVPIYLVSAATAYWHFLLLGLFIGLAGGAFSVGISYTARWFTKARQGFAMGIF
GAGNAGAAVTKFLAPSLVVAYGWQMVPKVYAVAMLVTAILFWAFTFTEHSHKVSSTVTIKDQLAALRDPKVWRYCQYYAI
VFGGYVGLSLWMTKYYINEYGFDIRTAALVAAIFVLPSGVIRALGGWLSDRFGAHTVTCWVMWVSGGCLLLLSIPHFDVV
VTTVNGPVARHVGLNVWVFTSLLFVVGIAWGFGKASVFKHISDEYPHNIGVISGIVGLIGGLGGFVLPIMFGALVDLTGV
RSTAFMLLYGSTCVSLFWMYLALKKENVHRKLKTADILE

Sequences:

>Translated_439_residues
MERHEQKTATAATWGTQVRTRTMATHRYKQISVLTMNTFAFTVCFAVWVMFSIIGIPIKGALGLNETQFGILAATPILSG
SLIRLPLGMWTDKYGGRIVFFILMLCTIVPIYLVSAATAYWHFLLLGLFIGLAGGAFSVGISYTARWFTKARQGFAMGIF
GAGNAGAAVTKFLAPSLVVAYGWQMVPKVYAVAMLVTAILFWAFTFTEHSHKVSSTVTIKDQLAALRDPKVWRYCQYYAI
VFGGYVGLSLWMTKYYINEYGFDIRTAALVAAIFVLPSGVIRALGGWLSDRFGAHTVTCWVMWVSGGCLLLLSIPHFDVV
VTTVNGPVARHVGLNVWVFTSLLFVVGIAWGFGKASVFKHISDEYPHNIGVISGIVGLIGGLGGFVLPIMFGALVDLTGV
RSTAFMLLYGSTCVSLFWMYLALKKENVHRKLKTADILE
>Mature_439_residues
MERHEQKTATAATWGTQVRTRTMATHRYKQISVLTMNTFAFTVCFAVWVMFSIIGIPIKGALGLNETQFGILAATPILSG
SLIRLPLGMWTDKYGGRIVFFILMLCTIVPIYLVSAATAYWHFLLLGLFIGLAGGAFSVGISYTARWFTKARQGFAMGIF
GAGNAGAAVTKFLAPSLVVAYGWQMVPKVYAVAMLVTAILFWAFTFTEHSHKVSSTVTIKDQLAALRDPKVWRYCQYYAI
VFGGYVGLSLWMTKYYINEYGFDIRTAALVAAIFVLPSGVIRALGGWLSDRFGAHTVTCWVMWVSGGCLLLLSIPHFDVV
VTTVNGPVARHVGLNVWVFTSLLFVVGIAWGFGKASVFKHISDEYPHNIGVISGIVGLIGGLGGFVLPIMFGALVDLTGV
RSTAFMLLYGSTCVSLFWMYLALKKENVHRKLKTADILE

Specific function: Probably required for nitrate uptake under anoxic conditions. Also possibly involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=433, Percent_Identity=25.8660508083141, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI1787475, Length=425, Percent_Identity=24, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 48167; Mature: 48167

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERHEQKTATAATWGTQVRTRTMATHRYKQISVLTMNTFAFTVCFAVWVMFSIIGIPIKG
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCC
ALGLNETQFGILAATPILSGSLIRLPLGMWTDKYGGRIVFFILMLCTIVPIYLVSAATAY
CCCCCCCCCHHEEECHHHCCCHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHFLLLGLFIGLAGGAFSVGISYTARWFTKARQGFAMGIFGAGNAGAAVTKFLAPSLVVA
HHHHHHHHHHHHCCCHHEECHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHH
YGWQMVPKVYAVAMLVTAILFWAFTFTEHSHKVSSTVTIKDQLAALRDPKVWRYCQYYAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEHHHHHHHCCCHHHHHHHHHHH
VFGGYVGLSLWMTKYYINEYGFDIRTAALVAAIFVLPSGVIRALGGWLSDRFGAHTVTCW
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VMWVSGGCLLLLSIPHFDVVVTTVNGPVARHVGLNVWVFTSLLFVVGIAWGFGKASVFKH
HHHHCCCEEEEEECCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
ISDEYPHNIGVISGIVGLIGGLGGFVLPIMFGALVDLTGVRSTAFMLLYGSTCVSLFWMY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LALKKENVHRKLKTADILE
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MERHEQKTATAATWGTQVRTRTMATHRYKQISVLTMNTFAFTVCFAVWVMFSIIGIPIKG
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCC
ALGLNETQFGILAATPILSGSLIRLPLGMWTDKYGGRIVFFILMLCTIVPIYLVSAATAY
CCCCCCCCCHHEEECHHHCCCHHEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHFLLLGLFIGLAGGAFSVGISYTARWFTKARQGFAMGIFGAGNAGAAVTKFLAPSLVVA
HHHHHHHHHHHHCCCHHEECHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHH
YGWQMVPKVYAVAMLVTAILFWAFTFTEHSHKVSSTVTIKDQLAALRDPKVWRYCQYYAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCEEEEHHHHHHHCCCHHHHHHHHHHH
VFGGYVGLSLWMTKYYINEYGFDIRTAALVAAIFVLPSGVIRALGGWLSDRFGAHTVTCW
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
VMWVSGGCLLLLSIPHFDVVVTTVNGPVARHVGLNVWVFTSLLFVVGIAWGFGKASVFKH
HHHHCCCEEEEEECCCCEEEEEECCCCCHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
ISDEYPHNIGVISGIVGLIGGLGGFVLPIMFGALVDLTGVRSTAFMLLYGSTCVSLFWMY
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
LALKKENVHRKLKTADILE
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA