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The map label for this gene is narK [H]

Identifier: 78221555

GI number: 78221555

Start: 373138

End: 374565

Strand: Direct

Name: narK [H]

Synonym: Gmet_0334

Alternate gene names: 78221555

Gene position: 373138-374565 (Clockwise)

Preceding gene: 78221554

Following gene: 78221556

Centisome position: 9.33

GC content: 63.73

Gene sequence:

>1428_bases
ATGCCAACGGACGTATTGACAGCAACGGAAACGAAAAAGAGTGCATCATCGCACGTTCTGACGGAGTGGACCCCGGAAGA
CCAGCATTTCTGGGAAACCAGGGGGAAGGCCATTGCCACCCGCAACCTCTGGATCTCGATCCCGGCCCTCTTCCTGGCCT
TTGCGGTCTGGATGGTCTGGAGCGTGGTCTGCGTGAACCTCCCCAACATCGGCTTCAAGTACGACACCAACAAGCTCTTC
TGGCTGACCGCCCTCCCCGCGCTTGCGGGGGCGACGCTCCGCATCTTCTACTCCTTCATGGTCCCCATCTTCGGGGGACG
CCGGTGGACCGCCATCAGCACGGCATCGCTCCTGATTCCCGCCATGGGGATCGGCTTTGCGGTACGGGACCCCGCGACCA
GCTACTCGACCATGCTGATCCTGGCCGTCCTCTGCGGGTTCGGCGGCGGCAACTTCGCCTCCAGCATGGCCAACATCAGC
TTCTTCTATCCCAAGGCCCTGAAGGGGACCGCCCTGGGGCTGAACGCCGGCCTGGGGAACCTGGGGGTGAGCGCCATGCA
GTTCCTCGTGCCGCTGGTTATCTCCACCGGCATGTTCGCCGCCCTCTGCGGCGATCCCCAGCAGTGCGTCATCAACGGGG
TGACCAAGCCTATGTGGCTCCAGAACGCCGGCTTCGTCTGGGTGCCGTTCATCGCCCTCTCCGCCCTGGTCGCCTGGTTC
GGCATGAACGACATCGCCAGCGCCAAGGCATCCTTCAGTGACCAGGCCATCATCTTCCGGCGCAAGCACAACTGGCTCAT
GTGCATCCTCTACCTCGGCACCTTCGGTTCGTTCATCGGCTACTCGGCGGGGCTCCCGCTCCTGACCAAGTCCCAGTTCC
CCGGCATCAACCCGGTCAAGTACGCCTTCCTCGGCCCCCTCGTGGGCGCACTGATCCGTCCCGTCGGTGGGTGGCTGGCC
GATAAGCTCGGTGGTGCGCGGGTCACCTTCTGGAACTTCATCATCATGGCCGGGGCCGTGTTCGGCGTGCTGGCGTTCCT
CCCCCACGGCGGAGTCGGCGGCAACTTCTGGGGATTCCTCGCCATGTTCATCCTCCTCTTCGCCACCACCGGCATCGGCA
ACGGCTCCACCTTCCGCATGATCCCGGTGATCTTCCTGACCGAGCGGCAGCGCGATGCGGCGGGAAGGGGGAAGGAGGCG
GAGGAGCAGGCAATCAAGGACGCCAACAAGGAGGCGGCCGCCGTCCTGGGCTTCACCTCGGCATTTGCGGCCTACGGCGG
GTTTTTCATCCCCAAGAGCTTCGGCACCTCCATTGCCGCCACCGGTGGCCCCCAGGTGGCCCTCTACGGGTTCATCGCCT
TCTACGCGAGCTGTATCATCCTCACCTGGTGGTATTATTCGCGTAAGAACGCTGAAGTCCCCTGCTAA

Upstream 100 bases:

>100_bases
CCTGGCCCTGAAGAAAGAAAACGTTCATCGCAAGCTGAAGACCGCCGACATTCTCGAATAACAGCCAATAACAGCCAATT
CCCATGGAAGGAGAATAACG

Downstream 100 bases:

>100_bases
TGCCGTTTCCCAGCAGGCATCTAACCAACAAAAGGAGAACAATGTGAAAAAAACAGCTATCACCATCGCCTTCGTCGCCA
CTTCTGCCTTTGCCGCACAT

Product: nitrate transporter

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MPTDVLTATETKKSASSHVLTEWTPEDQHFWETRGKAIATRNLWISIPALFLAFAVWMVWSVVCVNLPNIGFKYDTNKLF
WLTALPALAGATLRIFYSFMVPIFGGRRWTAISTASLLIPAMGIGFAVRDPATSYSTMLILAVLCGFGGGNFASSMANIS
FFYPKALKGTALGLNAGLGNLGVSAMQFLVPLVISTGMFAALCGDPQQCVINGVTKPMWLQNAGFVWVPFIALSALVAWF
GMNDIASAKASFSDQAIIFRRKHNWLMCILYLGTFGSFIGYSAGLPLLTKSQFPGINPVKYAFLGPLVGALIRPVGGWLA
DKLGGARVTFWNFIIMAGAVFGVLAFLPHGGVGGNFWGFLAMFILLFATTGIGNGSTFRMIPVIFLTERQRDAAGRGKEA
EEQAIKDANKEAAAVLGFTSAFAAYGGFFIPKSFGTSIAATGGPQVALYGFIAFYASCIILTWWYYSRKNAEVPC

Sequences:

>Translated_475_residues
MPTDVLTATETKKSASSHVLTEWTPEDQHFWETRGKAIATRNLWISIPALFLAFAVWMVWSVVCVNLPNIGFKYDTNKLF
WLTALPALAGATLRIFYSFMVPIFGGRRWTAISTASLLIPAMGIGFAVRDPATSYSTMLILAVLCGFGGGNFASSMANIS
FFYPKALKGTALGLNAGLGNLGVSAMQFLVPLVISTGMFAALCGDPQQCVINGVTKPMWLQNAGFVWVPFIALSALVAWF
GMNDIASAKASFSDQAIIFRRKHNWLMCILYLGTFGSFIGYSAGLPLLTKSQFPGINPVKYAFLGPLVGALIRPVGGWLA
DKLGGARVTFWNFIIMAGAVFGVLAFLPHGGVGGNFWGFLAMFILLFATTGIGNGSTFRMIPVIFLTERQRDAAGRGKEA
EEQAIKDANKEAAAVLGFTSAFAAYGGFFIPKSFGTSIAATGGPQVALYGFIAFYASCIILTWWYYSRKNAEVPC
>Mature_474_residues
PTDVLTATETKKSASSHVLTEWTPEDQHFWETRGKAIATRNLWISIPALFLAFAVWMVWSVVCVNLPNIGFKYDTNKLFW
LTALPALAGATLRIFYSFMVPIFGGRRWTAISTASLLIPAMGIGFAVRDPATSYSTMLILAVLCGFGGGNFASSMANISF
FYPKALKGTALGLNAGLGNLGVSAMQFLVPLVISTGMFAALCGDPQQCVINGVTKPMWLQNAGFVWVPFIALSALVAWFG
MNDIASAKASFSDQAIIFRRKHNWLMCILYLGTFGSFIGYSAGLPLLTKSQFPGINPVKYAFLGPLVGALIRPVGGWLAD
KLGGARVTFWNFIIMAGAVFGVLAFLPHGGVGGNFWGFLAMFILLFATTGIGNGSTFRMIPVIFLTERQRDAAGRGKEAE
EQAIKDANKEAAAVLGFTSAFAAYGGFFIPKSFGTSIAATGGPQVALYGFIAFYASCIILTWWYYSRKNAEVPC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=475, Percent_Identity=52.4210526315789, Blast_Score=463, Evalue=1e-131,
Organism=Escherichia coli, GI1787743, Length=468, Percent_Identity=52.5641025641026, Blast_Score=422, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 51230; Mature: 51099

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTDVLTATETKKSASSHVLTEWTPEDQHFWETRGKAIATRNLWISIPALFLAFAVWMVW
CCCCCEECCCCHHCCCCCEEEECCCCHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHH
SVVCVNLPNIGFKYDTNKLFWLTALPALAGATLRIFYSFMVPIFGGRRWTAISTASLLIP
HHHHHCCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
AMGIGFAVRDPATSYSTMLILAVLCGFGGGNFASSMANISFFYPKALKGTALGLNAGLGN
HHCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCEEECCCCCCCCEEEECCCCCH
LGVSAMQFLVPLVISTGMFAALCGDPQQCVINGVTKPMWLQNAGFVWVPFIALSALVAWF
HHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHH
GMNDIASAKASFSDQAIIFRRKHNWLMCILYLGTFGSFIGYSAGLPLLTKSQFPGINPVK
CCCHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHH
YAFLGPLVGALIRPVGGWLADKLGGARVTFWNFIIMAGAVFGVLAFLPHGGVGGNFWGFL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AMFILLFATTGIGNGSTFRMIPVIFLTERQRDAAGRGKEAEEQAIKDANKEAAAVLGFTS
HHHHHHHHHCCCCCCCCEEEEEEEEEECCCHHCCCCCCHHHHHHHHHCCCHHHHHHHHHH
AFAAYGGFFIPKSFGTSIAATGGPQVALYGFIAFYASCIILTWWYYSRKNAEVPC
HHHHHCCEECCHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
PTDVLTATETKKSASSHVLTEWTPEDQHFWETRGKAIATRNLWISIPALFLAFAVWMVW
CCCCEECCCCHHCCCCCEEEECCCCHHHHHHHCCCEEEECCCEEEHHHHHHHHHHHHHH
SVVCVNLPNIGFKYDTNKLFWLTALPALAGATLRIFYSFMVPIFGGRRWTAISTASLLIP
HHHHHCCCCCCEEECCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
AMGIGFAVRDPATSYSTMLILAVLCGFGGGNFASSMANISFFYPKALKGTALGLNAGLGN
HHCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCEEECCCCCCCCEEEECCCCCH
LGVSAMQFLVPLVISTGMFAALCGDPQQCVINGVTKPMWLQNAGFVWVPFIALSALVAWF
HHHHHHHHHHHHHHHHCHHHHHCCCHHHHHHCCCCCCCEECCCCCHHHHHHHHHHHHHHH
GMNDIASAKASFSDQAIIFRRKHNWLMCILYLGTFGSFIGYSAGLPLLTKSQFPGINPVK
CCCHHHHHHCCCCCCEEEEEECCCCEEHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHH
YAFLGPLVGALIRPVGGWLADKLGGARVTFWNFIIMAGAVFGVLAFLPHGGVGGNFWGFL
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AMFILLFATTGIGNGSTFRMIPVIFLTERQRDAAGRGKEAEEQAIKDANKEAAAVLGFTS
HHHHHHHHHCCCCCCCCEEEEEEEEEECCCHHCCCCCCHHHHHHHHHCCCHHHHHHHHHH
AFAAYGGFFIPKSFGTSIAATGGPQVALYGFIAFYASCIILTWWYYSRKNAEVPC
HHHHHCCEECCHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]